Status
Please wait ...
Gene information
General information
TC007436 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4506891..4508510
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007436
    iBeetle screen iB_09891

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007436 RA
      MSSFNEKLK LFSVELFRI NAIKFGDFK TKVGLQTPV 
      YVDLRVIVS YPKLLNDLA DLILEYQKQ LPKFNIICG 
      VPYTALPIA TVVSVKSDI PMVMRRKEA KDYGTKKLI 
      EGQFNEGDK CLIIEDVVT SGSSILETV RDLKNSGIN 
      CSDAIVLLN REQGGEQLL EKNGIKMHA LLTLTQLMA 
      FLNEANCVN DSTVEKVRM YLNSTQVDP SAIKTNNRL 
      KLPFSERAK LAKNAVSQQ LFKIMASKK TTLCVAADV 
      TSCTVLLNL AEEVGPHIC LLKTHIDVL EDFHPNILK 
      PLQEIADRH NFLLFEDRK FADIGQTVQ LQYSKGLYQ 
      ISSWASLVT AHSLMGKGV LDGLKQSPG LTNRGVFLL 
      AQTSAAESL IDRSYVDNT VKLARQYGD LVTGVVCQS 
      VLFEDEPGL VQLTPGVHL KSVGDNLSQ QYNTPEVVV 
      MDRGADVAV VGRGVTQAA SPGDEARKY KEVLWAAYE 
      KRINV
    CDS sequence
    • >TC007436 RA
      atgagttcg tttaacgag aaattaaaa ttattttct 
      gttgaattg ttccgaata aatgccatt aagttcggt 
      gattttaaa actaaagtt gggctccaa acccccgtc 
      tatgtcgat ttgagggta atcgtgagt taccctaaa 
      ttactgaac gaccttgcc gatttaatt ctcgagtac 
      cagaaacag ttacccaaa tttaacatt atttgtggg 
      gtcccttac accgcttta ccgatagcc accgtagtt 
      tcggtaaaa agtgatatt cctatggta atgaggagg 
      aaggaagcg aaagattac ggaacgaaa aaactgatt 
      gaaggtcaa tttaacgaa ggcgataaa tgtttaatt 
      atcgaggat gtcgttaca tccgggagc agtatttta 
      gaaacagtc cgagattta aaaaactca ggaattaat 
      tgctcggat gctattgtg ttactgaat agggaacaa 
      gggggcgag cagttgtta gaaaaaaac ggtattaaa 
      atgcacgct ttgctcacc ctgactcaa ctgatggcc 
      tttctgaat gaagcgaat tgtgtcaac gacagtacg 
      gttgaaaaa gtccgcatg tacttaaac agtactcaa 
      gtggacccc agtgccatt aaaacgaac aataggctc 
      aaattaccc ttctcagag cgagccaaa ctcgccaaa 
      aacgctgtt tcgcaacag ttgttcaaa ataatggct 
      tccaaaaaa acaacatta tgcgtggcc gcagacgtg 
      acttcctgc accgttttg ctgaatttg gccgaagag 
      gtgggccct catatttgc ctcctcaaa acccacatt 
      gacgtcctt gaggacttc caccccaac atcctcaaa 
      cccctccaa gaaatcgcc gaccggcac aacttcctc 
      ctctttgag gaccgaaaa ttcgccgat attggccaa 
      accgtgcaa ttgcagtac agtaaaggc ttgtaccag 
      atctcctca tgggctagt ctggtaacc gcccatagc 
      ttaatgggc aaaggtgtg cttgatggg ttaaaacaa 
      tccccgggt ttgacaaac cggggagtt ttccttttg 
      gcccaaaca agcgcggct gaaagtctc attgaccgg 
      agttatgtt gataatacg gttaaactg gcgagacag 
      tatggggat ttggtgact ggggtcgtt tgccagagt 
      gtgctattt gaggacgag ccggggctg gttcagctg 
      accccgggg gtacacttg aagtcagtc ggggataat 
      ttgagccag cagtacaac acaccagag gttgtggtc 
      atggacagg ggggctgac gtggccgtc gtgggccgt 
      ggggtgacc caagccgcc agtcctgga gacgaggcc 
      aggaaatat aaggaggtt ttgtgggcc gcttacgag 
      aaaaggatc aacgtgtga
    mRNA sequence
    • >TC007436 RA
      ccaggaatt agcgcaacc tttaagcac aacacagtt 
      tgagcaaca cgtggttgt tactaagat gagttcgtt 
      taacgagaa attaaaatt attttctgt tgaattgtt 
      ccgaataaa tgccattaa gttcggtga ttttaaaac 
      taaagttgg gctccaaac ccccgtcta tgtcgattt 
      gagggtaat cgtgagtta ccctaaatt actgaacga 
      ccttgccga tttaattct cgagtacca gaaacagtt 
      acccaaatt taacattat ttgtggggt cccttacac 
      cgctttacc gatagccac cgtagtttc ggtaaaaag 
      tgatattcc tatggtaat gaggaggaa ggaagcgaa 
      agattacgg aacgaaaaa actgattga aggtcaatt 
      taacgaagg cgataaatg tttaattat cgaggatgt 
      cgttacatc cgggagcag tattttaga aacagtccg 
      agatttaaa aaactcagg aattaattg ctcggatgc 
      tattgtgtt actgaatag ggaacaagg gggcgagca 
      gttgttaga aaaaaacgg tattaaaat gcacgcttt 
      gctcaccct gactcaact gatggcctt tctgaatga 
      agcgaattg tgtcaacga cagtacggt tgaaaaagt 
      ccgcatgta cttaaacag tactcaagt ggaccccag 
      tgccattaa aacgaacaa taggctcaa attaccctt 
      ctcagagcg agccaaact cgccaaaaa cgctgtttc 
      gcaacagtt gttcaaaat aatggcttc caaaaaaac 
      aacattatg cgtggccgc agacgtgac ttcctgcac 
      cgttttgct gaatttggc cgaagaggt gggccctca 
      tatttgcct cctcaaaac ccacattga cgtccttga 
      ggacttcca ccccaacat cctcaaacc cctccaaga 
      aatcgccga ccggcacaa cttcctcct ctttgagga 
      ccgaaaatt cgccgatat tggccaaac cgtgcaatt 
      gcagtacag taaaggctt gtaccagat ctcctcatg 
      ggctagtct ggtaaccgc ccatagctt aatgggcaa 
      aggtgtgct tgatgggtt aaaacaatc cccgggttt 
      gacaaaccg gggagtttt ccttttggc ccaaacaag 
      cgcggctga aagtctcat tgaccggag ttatgttga 
      taatacggt taaactggc gagacagta tggggattt 
      ggtgactgg ggtcgtttg ccagagtgt gctatttga 
      ggacgagcc ggggctggt tcagctgac cccgggggt 
      acacttgaa gtcagtcgg ggataattt gagccagca 
      gtacaacac accagaggt tgtggtcat ggacagggg 
      ggctgacgt ggccgtcgt gggccgtgg ggtgaccca 
      agccgccag tcctggaga cgaggccag gaaatataa 
      ggaggtttt gtgggccgc ttacgagaa aaggatcaa 
      cgtgtgatt tgttattgt taataaagc tgtaatatt 
      gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY