Status
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Gene information
General information
TC008067 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4498992..4506166
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008067
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09890

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008067 RA
      MVSIIKNQL LKHLSRFTK NLSADKINL STFKGEGEL 
      SNLELNEIV LTDLLELPS WLRLTKAWC NKVTFRIPW 
      TKLKSVPIH LSLDEVNIT VETCEELRS MSAQGGLSS 
      YAVPGKYSF IHKVIDGIT VTVNTVNIL FNSPAFTAS 
      VQLSRIVVE SKSPKWAKS DLRLTRLKD GEKGQLLIF 
      KELEWQTVR IEAKSTIDK NLTPLRLLT NQARCRIVI 
      KKRLSDCFI MGSRLVIIL DDLLWVLTD SQLKAALHF 
      LDSLAGLVQ KATQISRKA KAARKLEEF PEYQAQLAQ 
      EARSKGSAK PNIFTYYDV VETSYHFLS NQIVLHLCD 
      DPGAGRSAH PNLKDGGAL QIAVKRFQI DFYPYHLAK 
      GNRKHWPKY NDTAVPHTI WQEQALQAF KTKFFDLID 
      RNKMQHTPL SRARKMRNE SDGRVEFES GVPGRAYAP 
      ESPVDLRKP PSPSQKEMK QRIEFQLNK MMTTCVIMR 
      IEDFTLYKV TTSGRKQAL KEFIAGDRD HMTLPKDAS 
      IVHAEFIYY YYPSDIPFP LPPPKFYVH LNPVQIVFD 
      VDSCLWLNS FGLNLYQSL MSSKQPTGA SNYTYVDVK 
      IEAILPRLN FESTIDYPN QRDRPKTLS FQVTRVNIT 
      NVRSLEQSS RADLAKCVD SFHMGSLFF GTDFPSQPA 
      DFYVVTQKF LDHISANDN IRSVPNELD VSSLEALVE 
      QLSRELLWT EAKDVWCVN LDPVWGDFL GARAVGAAK 
      PVPFLDAVP VTVWLHTHM DPNSTIKIE KNMANADIH 
      ALAHISNLV SVQLNHYQY LFLLRLAED ASEMVTFLS 
      IDSNRILKV ESSGSVAVG ALLPQLEVT FVMPSQCPG 
      KESSGGDVE SFVPDSSSI ADDVNVGST ATVWQTSTL 
      SIHQDGAIR KNMANGSGQ IELNRSYSM EFTQSSPEI 
      KKSNQFANI NIQNNVNAG LSSMKKGFA NFMSSLDSA 
      LKPSPDDVS DTASIRSDA SSDSENYVM ISMETSDAA 
      ISDLMFNVK EFSLENTGP VEMASEVME DESTITTTS 
      DHSLTSSCR RKDLISVAT FKLNKVEFL QQSMGYSSS 
      IKVQVGNIT CEDCSSIPW DEFQSKFNS RARAWTDNP 
      TSNTTSPKV KLRLDHTLT LPSSKSMMQ LDFRDRDNL 
      KKLFRDLLT IKVSDLYLD LNMSTVTGL ADLAEDEII 
      PIPIPMHIS LDNVQVHLN EDRPPVNIT SPGPIPIDL 
      NVTQLYIMR STDGVFNIL PHKPNELSS SSSLASDQE 
      QKLGRLTSD NEELRRRLA AFERVSEEN RSLRKSEEE 
      TSVLRSCLA SAQDEVARL LDEKNKLLE EVKQLKSQM 
      ANRQLSGKR
    CDS sequence
    • >TC008067 RA
      atggtgtct atcataaaa aaccaactg ttgaaacat 
      ctttcacgg tttacgaaa aacctatcg gctgacaaa 
      attaacctg agcaccttt aaaggtgaa ggggaacta 
      tcaaacttg gaattgaac gaaatcgtt ttgactgat 
      ttactggaa ttgccttcc tggttgcgc ctgacgaag 
      gcctggtgc aacaaagtc accttccgc atcccttgg 
      acgaagctg aaaagcgtc ccaatccac ctgagcctt 
      gatgaggtg aacataacg gtggaaacg tgcgaggag 
      ctgcgctcg atgtcggcc caagggggg ctgtcgtcg 
      tacgcggtt cccggcaaa tacagcttc atccataaa 
      gtcatagac gggattaca gtgacggta aacaccgtt 
      aatatttta ttcaacagc cctgccttc acggctagt 
      gttcagttg tcccgaatc gtcgtggag tcaaagagc 
      cccaaatgg gcgaaaagc gacttacga ttgacgaga 
      ctcaaggac ggggagaaa ggccagttg ttgattttc 
      aaagagttg gagtggcag acggttcga atcgaggct 
      aaatcaacg attgataaa aacttgacg cccttgaga 
      ctcctcaca aaccaggcg cggtgtcgg atcgttatc 
      aagaaacga ctttcagac tgcttcata atgggctca 
      cgccttgtc ataatcctc gatgacttg ctctgggtc 
      ttaaccgac tcccagctg aaagcggcg cttcatttc 
      ctcgattcc ttggctgga cttgtccag aaagcgacc 
      caaatctca cgcaaggcc aaagccgcg cgaaaactc 
      gaagaattc ccggaatat caagcgcag ttggctcag 
      gaagcgcgc tctaaaggc tccgccaaa cccaacatt 
      ttcacttat tatgatgtg gttgaaacg tcttatcat 
      ttcttatca aatcagatt gttttgcac ttgtgtgac 
      gatccgggc gcgggccgc tccgcacac cccaatttg 
      aaagatggg ggcgctctc caaatcgca gtgaagagg 
      tttcaaatc gacttctac ccttatcat ttagccaag 
      ggcaaccgc aaacactgg cccaaatat aacgacact 
      gcggtgccc cacaccata tggcaggag caagcgctc 
      caagccttc aaaacgaag tttttcgac ctgattgat 
      cggaataaa atgcagcat acgccgtta agtcgggcc 
      aggaaaatg agaaacgag agcgatggt cgtgtggag 
      tttgagagt ggggtcccg ggccgagcg tacgccccg 
      gagtcgccc gtcgacctc aggaagccg cccagcccg 
      agtcagaag gaaatgaag caaaggatt gagttccaa 
      ttgaataag atgatgacg acttgtgtt ataatgcgg 
      atcgaagat tttacgctg tataaggtg acaacctct 
      gggagaaaa caggctttg aaagagttt attgccggt 
      gatcgtgat catatgacg ttacctaaa gatgcgagc 
      attgttcat gccgagttt atttactat tattatccg 
      agcgatata ccctttccg ttgcctccc ccgaaattc 
      tacgttcac ttaaacccc gtccaaatc gtcttcgac 
      gtcgactcc tgtctctgg ctcaactct ttcggtcta 
      aacctctac caatctctc atgtcgtcc aaacagcca 
      actggcgct tctaactac acttacgta gacgtgaaa 
      attgaagca attcttcca cggctcaac ttcgagagc 
      accattgac taccccaac caacgagac cgccccaaa 
      accctcagt tttcaagtc acaagagtc aacatcacg 
      aacgtgaga agtctcgag cagtcatcc agggccgac 
      ctagccaaa tgcgtcgat tcgttccac atggggtcc 
      ctatttttc ggcacggat tttccctca caaccggcc 
      gatttctac gtcgtgacc cagaaattt ctagaccat 
      atttcggcc aacgataac atacggagt gtccccaac 
      gagcttgac gtctcatcg cttgaagct ctggttgaa 
      caattgagc cgcgaattg ttatggacc gaggctaag 
      gacgtctgg tgcgtgaac cttgacccc gtttggggc 
      gacttcctg ggggcccgt gccgtgggg gcggccaaa 
      cctgtccca ttcctggac gctgtgccc gtcactgtc 
      tggctacac acccatatg gaccccaat tcgaccatc 
      aaaattgag aaaaatatg gcaaatgcc gacatacac 
      gccctggcc cacatctcg aatctagtc agcgtgcaa 
      ctcaaccac taccaatac ttgtttttg ttaagactg 
      gccgaagat gcctccgaa atggttact ttcctatca 
      attgactcg aaccggatt ttgaaagtt gagagcagt 
      gggagcgtt gccgtgggg gcgctctta ccccagctg 
      gaggtgacc ttcgttatg ccgtcgcag tgcccaggt 
      aaggagtct tcagggggc gacgtggag tctttcgtt 
      cctgattct tcgagtatc gctgatgac gtaaatgtg 
      ggctcgact gcgacagta tggcagact agcacactt 
      tcgatacat caagacggc gcgattcgg aaaaacatg 
      gcgaatggc tcgggtcag atcgagtta aatcgcagt 
      tattcgatg gaatttacc caaagtagc cccgaaatt 
      aaaaaatcg aaccagttc gccaacatt aacatacaa 
      aataatgtg aatgcgggg ttgtcttca atgaagaaa 
      ggtttcgcc aattttatg tcatcgcta gacagcgcg 
      cttaagccg agtcctgat gatgttagc gatacggcg 
      tcgatacgt agcgacgcg tctagtgat agtgaaaat 
      tacgtcatg ataagcatg gaaacgtca gacgcggcc 
      atttccgat ttgatgttt aatgtgaaa gaattttca 
      ctggaaaat acgggtcca gtggaaatg gccagcgaa 
      gtgatggaa gatgagagc acgatcacc acaactagt 
      gatcactcg ttgactagc agttgtagg cgaaaagat 
      ttgatttca gttgccaca tttaaatta aacaaagtc 
      gaatttttg caacaatca atgggttat tcgtcttcc 
      attaaagtg caagtcggg aatatcact tgtgaggat 
      tgtagttcg attccttgg gacgagttt cagagcaaa 
      ttcaactcg cgagcccgc gcctggacc gacaacccc 
      acaagcaac acaacatca ccgaaagtg aagctgcga 
      ctcgaccac actttaact ctccccagc tccaaatca 
      atgatgcag ttagatttc cgagaccga gacaacttg 
      aaaaaactc ttccgagat ttgctaaca attaaagtc 
      tccgatttg tacttggac ctaaacatg agtacggta 
      accgggttg gcggacttg gccgaggat gaaataatc 
      ccgatcccg atccctatg cacatctca ctggacaac 
      gtccaagtg cacctcaac gaagaccgc cccccggtt 
      aacataacg tccccaggc cccatcccg atcgattta 
      aacgtaact cagttgtat ataatgagg tctacggac 
      ggggttttc aatattttg ccccacaaa ccgaacgaa 
      ctgtcttcg agttcgtcc ctggcctcg gaccaggag 
      cagaaattg ggtcgcttg acctctgat aacgaggaa 
      ttgaggcgc cgtttggcg gcgttcgag cgagtttcg 
      gaggagaat cgctcgttg aggaaatct gaggaggaa 
      acgtcggtt ttgaggtcg tgcttggcc tcggcgcag 
      gacgaagtc gcgcggctt ttagacgag aaaaataag 
      ttactggag gaggtgaaa cagttgaaa agtcagatg 
      gcgaatcgg caactgagt ggcaagaga tag
    mRNA sequence
    • >TC008067 RA
      gtgtttttc taattaaat tactttatt aattaatta 
      accgtcgaa tccttctag tcatgtaaa aaacaccca 
      ataatcgta tccttgtag ttgtagttg tagttattt 
      aaaatggtg tctatcata aaaaaccaa ctgttgaaa 
      catctttca cggtttacg aaaaaccta tcggctgac 
      aaaattaac ctgagcacc tttaaaggt gaaggggaa 
      ctatcaaac ttggaattg aacgaaatc gttttgact 
      gatttactg gaattgcct tcctggttg cgcctgacg 
      aaggcctgg tgcaacaaa gtcaccttc cgcatccct 
      tggacgaag ctgaaaagc gtcccaatc cacctgagc 
      cttgatgag gtgaacata acggtggaa acgtgcgag 
      gagctgcgc tcgatgtcg gcccaaggg gggctgtcg 
      tcgtacgcg gttcccggc aaatacagc ttcatccat 
      aaagtcata gacgggatt acagtgacg gtaaacacc 
      gttaatatt ttattcaac agccctgcc ttcacggct 
      agtgttcag ttgtcccga atcgtcgtg gagtcaaag 
      agccccaaa tgggcgaaa agcgactta cgattgacg 
      agactcaag gacggggag aaaggccag ttgttgatt 
      ttcaaagag ttggagtgg cagacggtt cgaatcgag 
      gctaaatca acgattgat aaaaacttg acgcccttg 
      agactcctc acaaaccag gcgcggtgt cggatcgtt 
      atcaagaaa cgactttca gactgcttc ataatgggc 
      tcacgcctt gtcataatc ctcgatgac ttgctctgg 
      gtcttaacc gactcccag ctgaaagcg gcgcttcat 
      ttcctcgat tccttggct ggacttgtc cagaaagcg 
      acccaaatc tcacgcaag gccaaagcc gcgcgaaaa 
      ctcgaagaa ttcccggaa tatcaagcg cagttggct 
      caggaagcg cgctctaaa ggctccgcc aaacccaac 
      attttcact tattatgat gtggttgaa acgtcttat 
      catttctta tcaaatcag attgttttg cacttgtgt 
      gacgatccg ggcgcgggc cgctccgca caccccaat 
      ttgaaagat gggggcgct ctccaaatc gcagtgaag 
      aggtttcaa atcgacttc tacccttat catttagcc 
      aagggcaac cgcaaacac tggcccaaa tataacgac 
      actgcggtg ccccacacc atatggcag gagcaagcg 
      ctccaagcc ttcaaaacg aagtttttc gacctgatt 
      gatcggaat aaaatgcag catacgccg ttaagtcgg 
      gccaggaaa atgagaaac gagagcgat ggtcgtgtg 
      gagtttgag agtggggtc ccgggccga gcgtacgcc 
      ccggagtcg cccgtcgac ctcaggaag ccgcccagc 
      ccgagtcag aaggaaatg aagcaaagg attgagttc 
      caattgaat aagatgatg acgacttgt gttataatg 
      cggatcgaa gattttacg ctgtataag gtgacaacc 
      tctgggaga aaacaggct ttgaaagag tttattgcc 
      ggtgatcgt gatcatatg acgttacct aaagatgcg 
      agcattgtt catgccgag tttatttac tattattat 
      ccgagcgat atacccttt ccgttgcct cccccgaaa 
      ttctacgtt cacttaaac cccgtccaa atcgtcttc 
      gacgtcgac tcctgtctc tggctcaac tctttcggt 
      ctaaacctc taccaatct ctcatgtcg tccaaacag 
      ccaactggc gcttctaac tacacttac gtagacgtg 
      aaaattgaa gcaattctt ccacggctc aacttcgag 
      agcaccatt gactacccc aaccaacga gaccgcccc 
      aaaaccctc agttttcaa gtcacaaga gtcaacatc 
      acgaacgtg agaagtctc gagcagtca tccagggcc 
      gacctagcc aaatgcgtc gattcgttc cacatgggg 
      tccctattt ttcggcacg gattttccc tcacaaccg 
      gccgatttc tacgtcgtg acccagaaa tttctagac 
      catatttcg gccaacgat aacatacgg agtgtcccc 
      aacgagctt gacgtctca tcgcttgaa gctctggtt 
      gaacaattg agccgcgaa ttgttatgg accgaggct 
      aaggacgtc tggtgcgtg aaccttgac cccgtttgg 
      ggcgacttc ctgggggcc cgtgccgtg ggggcggcc 
      aaacctgtc ccattcctg gacgctgtg cccgtcact 
      gtctggcta cacacccat atggacccc aattcgacc 
      atcaaaatt gagaaaaat atggcaaat gccgacata 
      cacgccctg gcccacatc tcgaatcta gtcagcgtg 
      caactcaac cactaccaa tacttgttt ttgttaaga 
      ctggccgaa gatgcctcc gaaatggtt actttccta 
      tcaattgac tcgaaccgg attttgaaa gttgagagc 
      agtgggagc gttgccgtg ggggcgctc ttaccccag 
      ctggaggtg accttcgtt atgccgtcg cagtgccca 
      ggtaaggag tcttcaggg ggcgacgtg gagtctttc 
      gttcctgat tcttcgagt atcgctgat gacgtaaat 
      gtgggctcg actgcgaca gtatggcag actagcaca 
      ctttcgata catcaagac ggcgcgatt cggaaaaac 
      atggcgaat ggctcgggt cagatcgag ttaaatcgc 
      agttattcg atggaattt acccaaagt agccccgaa 
      attaaaaaa tcgaaccag ttcgccaac attaacata 
      caaaataat gtgaatgcg gggttgtct tcaatgaag 
      aaaggtttc gccaatttt atgtcatcg ctagacagc 
      gcgcttaag ccgagtcct gatgatgtt agcgatacg 
      gcgtcgata cgtagcgac gcgtctagt gatagtgaa 
      aattacgtc atgataagc atggaaacg tcagacgcg 
      gccatttcc gatttgatg tttaatgtg aaagaattt 
      tcactggaa aatacgggt ccagtggaa atggccagc 
      gaagtgatg gaagatgag agcacgatc accacaact 
      agtgatcac tcgttgact agcagttgt aggcgaaaa 
      gatttgatt tcagttgcc acatttaaa ttaaacaaa 
      gtcgaattt ttgcaacaa tcaatgggt tattcgtct 
      tccattaaa gtgcaagtc gggaatatc acttgtgag 
      gattgtagt tcgattcct tgggacgag tttcagagc 
      aaattcaac tcgcgagcc cgcgcctgg accgacaac 
      cccacaagc aacacaaca tcaccgaaa gtgaagctg 
      cgactcgac cacacttta actctcccc agctccaaa 
      tcaatgatg cagttagat ttccgagac cgagacaac 
      ttgaaaaaa ctcttccga gatttgcta acaattaaa 
      gtctccgat ttgtacttg gacctaaac atgagtacg 
      gtaaccggg ttggcggac ttggccgag gatgaaata 
      atcccgatc ccgatccct atgcacatc tcactggac 
      aacgtccaa gtgcacctc aacgaagac cgccccccg 
      gttaacata acgtcccca ggccccatc ccgatcgat 
      ttaaacgta actcagttg tatataatg aggtctacg 
      gacggggtt ttcaatatt ttgccccac aaaccgaac 
      gaactgtct tcgagttcg tccctggcc tcggaccag 
      gagcagaaa ttgggtcgc ttgacctct gataacgag 
      gaattgagg cgccgtttg gcggcgttc gagcgagtt 
      tcggaggag aatcgctcg ttgaggaaa tctgaggag 
      gaaacgtcg gttttgagg tcgtgcttg gcctcggcg 
      caggacgaa gtcgcgcgg cttttagac gagaaaaat 
      aagttactg gaggaggtg aaacagttg aaaagtcag 
      atggcgaat cggcaactg agtggcaag agatagcaa 
      tgctgtttt gactgcaca aactgctgc tctaacgtt 
      aaaaaaact taggcgtag tttagtcag tcgttttag 
      ttaggtgtg cgtagttta gcgctttat tgaaggctt 
      gatgtttat tcaaacttg tttttggca cagagaatc 
      ggggtaaac ttgcaataa ttacgttga tatttactt 
      tattttgtg ctgtgacgt aattttaag tgtaaatat 
      atgtctggt catgtaaat aaaggcgct tggattgat 
      aatgccaaa gaacgtttg aataattgt tcttcactc 
      atgtatttc ttccatcca ctttaatta ggatcgtga 
      cacttttta gagactggg tttcattac tgttacata 
      aagac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY