Status
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Gene information
General information
TC008059 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4415862..4418070
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008059
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09889

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008059 RA
      MANVRSLIG ILCTKQLNI FVTPQVNKI SARFLHNCL 
      HCNKFSVNF NVTNSPVKN VLHISSRKI HLNPVLRAA 
      KDYYQILGV SRNASGAEI KKAYYKLAK KYHPDVNKD 
      DPNAAKKFQ EVSEAYEIL GDETKRKQF DTFGATSEQ 
      MGGMGSGGG PRPGAQGFA ESWQYQSTI NPEELFRKI 
      FGEAGFGKS GGFDDFAES NFGFGQAQE VVVKLSFLQ 
      AARGTNKDI TVNVVDTCP KCHGSRCEP GTKATRCTH 
      CDGTGMESV TRGPFIMRS TCRYCQGSR MFIKHKCVE 
      CEGKGQTVQ RKRITVPVP AGIEDGQTV RMSVGQKEL 
      FITFHIEKS RYFKRDGAD VHTEAEISV AQALLGGTI 
      RIEGLYEDH MIQVMPGTS SHTRIRLAG KGMKKVDGY 
      GHGDHYVTF KIAVPKQLS PKQKALAQA YAELETDTP 
      GQILGVTLK TDGSKIFVS KDKQELLEA LRLVLQGKK 
      VETAKENHS QKNTKKVVD RDVDNENVS DTEEAKRNK 
      MP
    CDS sequence
    • >TC008059 RA
      atggcgaat gtcagaagt cttattggc attttgtgt 
      acaaaacag ttaaatatc ttcgtgaca cctcaagta 
      aacaaaatt tcagcaaga tttctacac aattgtctc 
      cactgtaac aaattttcg gtcaatttc aatgtcaca 
      aactctccg gtaaaaaac gttttacac atttcatcc 
      cgaaaaatc cacctaaac ccggttttg cgtgcggcg 
      aaagactat taccaaatt ctgggggtt agtcgtaac 
      gcatctggg gccgaaata aagaaggct tattataaa 
      ctagcgaaa aagtatcat cccgatgtc aataaagat 
      gacccaaat gcggctaaa aaattccaa gaggtgtcc 
      gaagcgtac gagatactt ggcgacgaa accaaacgg 
      aagcaattt gacacgttt ggcgccacg tccgagcag 
      atgggcggc atggggagc ggcggtggc ccccgtccc 
      ggcgcccaa ggcttcgcc gaaagctgg caataccag 
      tcaaccatt aacccggaa gaactcttc cggaaaatt 
      ttcggagag gccggtttt ggcaaaagc gggggattt 
      gatgatttt gctgaatcg aattttggg tttggacaa 
      gcacaagag gttgtggtt aaattgagc ttcttgcaa 
      gccgcaagg gggactaat aaggatatt actgtcaat 
      gttgtggac acctgtccc aagtgtcac ggctcgagg 
      tgcgaacca ggcaccaag gccacacgg tgcacacat 
      tgtgatggg actgggatg gaaagtgtg acccggggg 
      ccctttatt atgcggtcc acttgtagg tattgccaa 
      ggaagtcgt atgtttatc aaacataag tgtgtcgag 
      tgtgaggga aagggacaa acggtgcaa aggaaaagg 
      atcacggtg ccagtacca gcaggtatt gaagacggt 
      caaactgtg agaatgtcg gtagggcaa aaagaattg 
      tttataacg tttcatatt gaaaaatcg cgctatttc 
      aaacgagac ggggctgat gtacacacg gaggccgaa 
      atttccgtg gcgcaggca cttttaggg ggcaccatc 
      cgtatagaa gggttgtac gaagaccac atgatacag 
      gtaatgccg ggaacgtcg tcacacacc cgaataaga 
      cttgccggc aaaggcatg aaaaaagta gatggttac 
      ggtcatggt gaccattat gtcactttc aaaatagct 
      gtaccaaaa caactttct cctaaacaa aaagcccta 
      gcccaggca tatgccgag ctggaaact gatactccg 
      ggacaaatt ttaggagtg acgttaaaa actgatggt 
      agcaaaatt tttgtctcg aaagacaaa caagaactt 
      ttagaagcc cttaggtta gtgctacaa ggtaagaaa 
      gtcgaaacc gccaaagaa aatcactca caaaaaaac 
      actaagaaa gttgtagat agagatgtt gataatgaa 
      aatgttagc gacactgaa gaagcaaaa agaaataaa 
      atgccataa
    mRNA sequence
    • >TC008059 RA
      attcattga ttgacagtg ttaccacca cgctctgcc 
      ttttcctaa aaaaatggc gaatgtcag aagtcttat 
      tggcatttt gtgtacaaa acagttaaa tatcttcgt 
      gacacctca agtaaacaa aatttcagc aagatttct 
      acacaattg tctccactg taacaaatt ttcggtcaa 
      tttcaatgt cacaaactc tccggtaaa aaacgtttt 
      acacatttc atcccgaaa aatccacct aaacccggt 
      tttgcgtgc ggcgaaaga ctattacca aattctggg 
      ggttagtcg taacgcatc tggggccga aataaagaa 
      ggcttatta taaactagc gaaaaagta tcatcccga 
      tgtcaataa agatgaccc aaatgcggc taaaaaatt 
      ccaagaggt gtccgaagc gtacgagat acttggcga 
      cgaaaccaa acggaagca atttgacac gtttggcgc 
      cacgtccga gcagatggg cggcatggg gagcggcgg 
      tggcccccg tcccggcgc ccaaggctt cgccgaaag 
      ctggcaata ccagtcaac cattaaccc ggaagaact 
      cttccggaa aattttcgg agaggccgg ttttggcaa 
      aagcggggg atttgatga ttttgctga atcgaattt 
      tgggtttgg acaagcaca agaggttgt ggttaaatt 
      gagcttctt gcaagccgc aagggggac taataagga 
      tattactgt caatgttgt ggacacctg tcccaagtg 
      tcacggctc gaggtgcga accaggcac caaggccac 
      acggtgcac acattgtga tgggactgg gatggaaag 
      tgtgacccg ggggccctt tattatgcg gtccacttg 
      taggtattg ccaaggaag tcgtatgtt tatcaaaca 
      taagtgtgt cgagtgtga gggaaaggg acaaacggt 
      gcaaaggaa aaggatcac ggtgccagt accagcagg 
      tattgaaga cggtcaaac tgtgagaat gtcggtagg 
      gcaaaaaga attgtttat aacgtttca tattgaaaa 
      atcgcgcta tttcaaacg agacggggc tgatgtaca 
      cacggaggc cgaaatttc cgtggcgca ggcactttt 
      agggggcac catccgtat agaagggtt gtacgaaga 
      ccacatgat acaggtaat gccgggaac gtcgtcaca 
      cacccgaat aagacttgc cggcaaagg catgaaaaa 
      agtagatgg ttacggtca tggtgacca ttatgtcac 
      tttcaaaat agctgtacc aaaacaact ttctcctaa 
      acaaaaagc cctagccca ggcatatgc cgagctgga 
      aactgatac tccgggaca aattttagg agtgacgtt 
      aaaaactga tggtagcaa aatttttgt ctcgaaaga 
      caaacaaga acttttaga agcccttag gttagtgct 
      acaaggtaa gaaagtcga aaccgccaa agaaaatca 
      ctcacaaaa aaacactaa gaaagttgt agatagaga 
      tgttgataa tgaaaatgt tagcgacac tgaagaagc 
      aaaaagaaa taaaatgcc ataactgtt ttgttaact 
      agttttata ttaaaatgt tattctaaa gaaagaata 
      aaccgacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY