Status
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Gene information
General information
TC008045 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4269882..4275726
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008045
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09887

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008045 RA
      MAWRFKASK YKNAAPIVP KPENYVRDI CVGSYQTYG 
      NNISASAKF MAFNWDHAG SSLAVLPLD DSGRKSKLM 
      PLLHAHSDT VTDLEFSPF HDGLLATGS QDCLVKIWH 
      IPETGLQES LSNPECTFS HKQRRVETV GFHPTADFL 
      LHSTSYTTL TLWDLISGQ EVFSNAEPS DVIQSVSWK 
      RDGTLVATS SKDKQVRIL DPRVEQACV KTANSHQSI 
      KDSRIVWLG DSNRVLTTG FDAQRLRQI IVRDLRNFG 
      EAEKSIELD CSTGILIPL YDADTGMLF LAGKGDTTI 
      GYMEVTDKE PYLTEGIRH SGEQTKGAC LVPKRALNV 
      MQGEVNRIL QLTSTCVIP ITYQVPRKT YRDFHADLY 
      PDTPGYKTE LTAAQWLNG KNIPLPKIS LDPSKRENG 
      EEPIIVHRG PLSKLREEY ENEKVIEPK PVPKKPFQP 
      KITATITKN DFHPVKEIL KDFEKPEIQ DNKVAENNK 
      INLEETAVP NVVPPKPLP RTSRTGSIC EPLEDQNNP 
      PKPVARPRT NSATPIVTS VNPNVPIAG GYKPRLGPK 
      PFTPPKLNE SESSFDKVF SVPAVPSQP QQSNGVHEP 
      KTPDSQPPT PSQPDKEIE EEAPEPPKT PSTAERRKL 
      FESNTKENE PEDNFDVID QSGNFERAS PQRSSIAER 
      RKMYENRSQ SVQETPTII EKAGGSPVM LRRKDSFKN 
      RKNTEDVLK EDNNRKSGQ VSKQMSLEQ SARKSENFA 
      TPTPKRTST VFGRVSKFR HLKGTPAHK SLHIENIRN 
      LSRQISGEC DGFHANPDR VAVPLSGPG GKIAIFELS 
      KTGKLPDGV IPALVHGSN IMDFAWDPF DNQRLAVAC 
      DDGVIKLWK IPETGLSEP TNEAEAEFN AHPDKIYFI 
      KFHPTARDV LASGSYDMT IKIWDLATL SEKIVLRGH 
      TDQMFSFAW SPCGAFCST VSKDGKIRI YKPRAGDLP 
      IREGKGPVG SRGARIVWA LDGGFIVVT GFDKVSERQ 
      IMVFKATDL SNPLNTIGL DVSPAILIP FYDEDSSTL 
      FVTGKGDLT IYAFEITEE SPYICPLSH HRCNNLHQG 
      LSFLPKNVC DVSNVEFAK ALRLTNNTI EPLSFTVPR 
      IKTELFQDD LFPPTKVTW KPSLSSTEW YNNKDKTPQ 
      RISLKPEGM DLLSESQGQ TNPERTINK SASTPFIGA 
      SQPYNRLAW NADLNEKTK MKQEELQKS VSNRMEVNL 
      KLEQDEMEG VDEKEWTD
    CDS sequence
    • >TC008045 RA
      atggcttgg cgcttcaaa gcttcgaaa tacaaaaac 
      gcggccccc attgtcccc aaaccagag aactacgtg 
      cgcgatatc tgcgtgggg tcttaccag acctatggg 
      aacaacatc tcagcttcg gccaaattc atggccttt 
      aattgggac cacgccggt tcgagtctc gccgtcttg 
      cccttggac gactcgggc cgcaagagc aagctcatg 
      cctctcttg cacgcgcat tcggacacc gtcaccgac 
      ttggagttc tcgccgttt cacgacggg ttactggcc 
      accgggtcg caggactgt ctcgtcaaa atctggcat 
      atccccgaa acggggctc caggagtcg ctcagtaac 
      cccgagtgc acgttctcg cacaagcag cgtcgagtg 
      gagaccgtg ggcttccac cccacggcc gatttcctc 
      ctccactcg acctcgtac accacgttg accctctgg 
      gacttgatc tcgggccag gaggtgttt tccaacgct 
      gaaccctcc gacgttatc caatcggtg agttggaaa 
      cgggacgga actctggtt gcgacctct tcgaaagac 
      aaacaagtg cgaattttg gacccgagg gtcgagcaa 
      gcgtgcgtt aaaacggcc aatagccac caaagtatc 
      aaagattcg aggattgtc tggcttggt gatagtaat 
      agggttttg acgacgggt ttcgacgcg caaagactg 
      cgacaaatc atcgtacgt gatttgaga aatttcggc 
      gaagcggaa aaaagcata gaacttgat tgttctacc 
      ggaattctt attccgctc tatgacgcc gataccggg 
      atgttgttc ctggccgga aaaggtgat accacgatt 
      gggtacatg gaagttacg gacaaggag ccgtatttg 
      accgaagga atacggcat tcaggggaa caaaccaag 
      ggggcttgt cttgtaccc aagcgagcc ctcaatgtt 
      atgcaaggc gaagtcaat aggattttg cagttgaca 
      agcacttgt gttattccg attacgtat caagtaccc 
      aggaaaaca tatagggac ttccacgct gacttgtat 
      cccgatacg cccggatac aagactgag ttgacggcc 
      gctcagtgg ttgaacggc aaaaacatt ccactcccg 
      aaaattagt ttggatcct tcaaagcgc gaaaatggc 
      gaagaacca atcatagta catagagga cccctttcg 
      aaactccga gaagagtac gaaaacgaa aaagtaatc 
      gaaccaaaa cccgtcccc aaaaaaccc ttccaacct 
      aaaatcacg gcgacgata acgaaaaac gatttccat 
      ccggtaaag gaaattcta aaagatttc gaaaagccg 
      gaaattcaa gataataaa gttgcggaa aataacaag 
      attaattta gaagaaaca gcagtaccg aatgtcgta 
      ccgccaaaa cccttaccg cgcacctct cgaaccggt 
      tccatttgc gaaccactg gaagaccaa aataacccc 
      cccaaacca gtcgctaga cctcggacg aacagcgcg 
      acacctatc gtcacatcg gttaacccc aatgtgccc 
      atagcagga ggatacaag cctcgtttg ggtccaaag 
      cccttcact cctccaaaa ctcaacgag tctgaaagt 
      agcttcgac aaagttttt tcggtgcca gccgtccca 
      agccaaccg caacaaagt aacggagtg cacgaaccg 
      aaaacaccc gactcacaa cccccaact ccttcacaa 
      cccgacaaa gaaatcgag gaggaggca cccgaaccc 
      cccaaaacg ccaagtacg gccgaacga aggaaactc 
      ttcgaaagc aacactaaa gagaacgaa ccggaagat 
      aacttcgac gttatcgac cagagcgga aactttgaa 
      agggcctcg ccacaaagg agtagcatc gctgagagg 
      aggaaaatg tacgaaaat cgatcgcaa tcggtgcag 
      gaaacaccg accattatt gagaaggcc ggggggtca 
      ccggtgatg ctgcggcgg aaggattcg ttcaaaaat 
      aggaaaaat acagaggat gtcctcaaa gaggataac 
      aataggaag agcggacag gtgtccaaa cagatgtcg 
      ctcgagcag agcgccagg aaaagcgag aattttgcg 
      actccgacg ccgaagagg acctccaca gtgtttggt 
      cgggtgtcg aagttccgc cacctgaaa ggcacgcct 
      gcccacaaa tcgctccac atcgagaac atccgtaac 
      ctcagccgc caaatctcg ggtgaatgt gacggcttc 
      cacgcaaac cctgaccgt gtggcggtg cctctgagt 
      ggccccggc ggtaaaatc gccattttc gagttgagc 
      aaaacgggt aaattaccc gatggggtg attccagcc 
      cttgtccat ggcagtaac atcatggac ttcgcttgg 
      gatcccttc gacaaccaa cgcctggct gtggcgtgc 
      gatgacggc gttatcaag ctgtggaaa atccccgaa 
      acggggctc agtgagccg acgaacgag gcggaagcc 
      gagtttaac gctcatcct gataagatt tactttatc 
      aagtttcat cccaccgct agggatgtc ttggcctcg 
      ggatcgtac gacatgacg atcaaaatt tgggatttg 
      gcgactttg agcgagaag attgtcttg agggggcac 
      acggatcag atgtttagt tttgcgtgg tcgccatgt 
      ggggcgttt tgttcgacg gtgagcaaa gacgggaag 
      atcaggatt tataagcca cgggccggg gatttaccc 
      attagggag gggaaaggg ccggttggg agtcgcggg 
      gcgaggatt gtttgggcc ctggatggc gggtttatc 
      gtcgttacg ggatttgat aaagtctcc gagagacaa 
      atcatggtt ttcaaagcc actgatttg tcaaatccc 
      ttaaatacg attggtctg gacgtgtcc ccggctatc 
      ttgattccg ttttacgat gaggacagc tcgactttg 
      ttcgtgacg gggaagggc gatttgacg atttatgcg 
      ttcgaaatt acggaggaa tcgccctat atatgcccc 
      ttaagccac cacaggtgc aataatcta caccaaggt 
      ctttcattt ttgccaaaa aatgtgtgt gatgtcagt 
      aatgtcgaa tttgcgaaa gcgctaaga ctcactaat 
      aatacgata gagccgttg tctttcacc gttccaagg 
      attaaaacg gaactcttc caagacgac ttgttcccc 
      ccgaccaag gtcacgtgg aaaccaagt ttgtcaagt 
      acagagtgg tacaataac aaagataaa accccacaa 
      aggatcagt ctcaagcca gaaggcatg gatttgtta 
      tccgagtct caaggccag actaatcct gaaaggacc 
      ataaacaag tctgcttcc actcctttc attggagcc 
      tctcagcct tataacagg ttagcgtgg aacgccgat 
      ttgaacgaa aaaactaaa atgaaacaa gaggagttg 
      cagaaatct gtgagtaac aggatggag gtcaacttg 
      aagttggaa caggacgag atggaaggc gttgatgaa 
      aaagaatgg accgattaa
    mRNA sequence
    • >TC008045 RA
      atgcattgt cacatctgt caatagtag tgttttggg 
      tgcgtttcg ggtgtagaa ccgctattt cgtgcgtaa 
      tctcctcct ttaagttaa atgcagtta aaattgcgt 
      taaacctgc gtaaaaacc cgtcgtttc attcaacgc 
      atagtcatg gcttggcgc ttcaaagct tcgaaatac 
      aaaaacgcg gcccccatt gtccccaaa ccagagaac 
      tacgtgcgc gatatctgc gtggggtct taccagacc 
      tatgggaac aacatctca gcttcggcc aaattcatg 
      gcctttaat tgggaccac gccggttcg agtctcgcc 
      gtcttgccc ttggacgac tcgggccgc aagagcaag 
      ctcatgcct ctcttgcac gcgcattcg gacaccgtc 
      accgacttg gagttctcg ccgtttcac gacgggtta 
      ctggccacc gggtcgcag gactgtctc gtcaaaatc 
      tggcatatc cccgaaacg gggctccag gagtcgctc 
      agtaacccc gagtgcacg ttctcgcac aagcagcgt 
      cgagtggag accgtgggc ttccacccc acggccgat 
      ttcctcctc cactcgacc tcgtacacc acgttgacc 
      ctctgggac ttgatctcg ggccaggag gtgttttcc 
      aacgctgaa ccctccgac gttatccaa tcggtgagt 
      tggaaacgg gacggaact ctggttgcg acctcttcg 
      aaagacaaa caagtgcga attttggac ccgagggtc 
      gagcaagcg tgcgttaaa acggccaat agccaccaa 
      agtatcaaa gattcgagg attgtctgg cttggtgat 
      agtaatagg gttttgacg acgggtttc gacgcgcaa 
      agactgcga caaatcatc gtacgtgat ttgagaaat 
      ttcggcgaa gcggaaaaa agcatagaa cttgattgt 
      tctaccgga attcttatt ccgctctat gacgccgat 
      accgggatg ttgttcctg gccggaaaa ggtgatacc 
      acgattggg tacatggaa gttacggac aaggagccg 
      tatttgacc gaaggaata cggcattca ggggaacaa 
      accaagggg gcttgtctt gtacccaag cgagccctc 
      aatgttatg caaggcgaa gtcaatagg attttgcag 
      ttgacaagc acttgtgtt attccgatt acgtatcaa 
      gtacccagg aaaacatat agggacttc cacgctgac 
      ttgtatccc gatacgccc ggatacaag actgagttg 
      acggccgct cagtggttg aacggcaaa aacattcca 
      ctcccgaaa attagtttg gatccttca aagcgcgaa 
      aatggcgaa gaaccaatc atagtacat agaggaccc 
      ctttcgaaa ctccgagaa gagtacgaa aacgaaaaa 
      gtaatcgaa ccaaaaccc gtccccaaa aaacccttc 
      caacctaaa atcacggcg acgataacg aaaaacgat 
      ttccatccg gtaaaggaa attctaaaa gatttcgaa 
      aagccggaa attcaagat aataaagtt gcggaaaat 
      aacaagatt aatttagaa gaaacagca gtaccgaat 
      gtcgtaccg ccaaaaccc ttaccgcgc acctctcga 
      accggttcc atttgcgaa ccactggaa gaccaaaat 
      aaccccccc aaaccagtc gctagacct cggacgaac 
      agcgcgaca cctatcgtc acatcggtt aaccccaat 
      gtgcccata gcaggagga tacaagcct cgtttgggt 
      ccaaagccc ttcactcct ccaaaactc aacgagtct 
      gaaagtagc ttcgacaaa gttttttcg gtgccagcc 
      gtcccaagc caaccgcaa caaagtaac ggagtgcac 
      gaaccgaaa acacccgac tcacaaccc ccaactcct 
      tcacaaccc gacaaagaa atcgaggag gaggcaccc 
      gaacccccc aaaacgcca agtacggcc gaacgaagg 
      aaactcttc gaaagcaac actaaagag aacgaaccg 
      gaagataac ttcgacgtt atcgaccag agcggaaac 
      tttgaaagg gcctcgcca caaaggagt agcatcgct 
      gagaggagg aaaatgtac gaaaatcga tcgcaatcg 
      gtgcaggaa acaccgacc attattgag aaggccggg 
      gggtcaccg gtgatgctg cggcggaag gattcgttc 
      aaaaatagg aaaaataca gaggatgtc ctcaaagag 
      gataacaat aggaagagc ggacaggtg tccaaacag 
      atgtcgctc gagcagagc gccaggaaa agcgagaat 
      tttgcgact ccgacgccg aagaggacc tccacagtg 
      tttggtcgg gtgtcgaag ttccgccac ctgaaaggc 
      acgcctgcc cacaaatcg ctccacatc gagaacatc 
      cgtaacctc agccgccaa atctcgggt gaatgtgac 
      ggcttccac gcaaaccct gaccgtgtg gcggtgcct 
      ctgagtggc cccggcggt aaaatcgcc attttcgag 
      ttgagcaaa acgggtaaa ttacccgat ggggtgatt 
      ccagccctt gtccatggc agtaacatc atggacttc 
      gcttgggat cccttcgac aaccaacgc ctggctgtg 
      gcgtgcgat gacggcgtt atcaagctg tggaaaatc 
      cccgaaacg gggctcagt gagccgacg aacgaggcg 
      gaagccgag tttaacgct catcctgat aagatttac 
      tttatcaag tttcatccc accgctagg gatgtcttg 
      gcctcggga tcgtacgac atgacgatc aaaatttgg 
      gatttggcg actttgagc gagaagatt gtcttgagg 
      gggcacacg gatcagatg tttagtttt gcgtggtcg 
      ccatgtggg gcgttttgt tcgacggtg agcaaagac 
      gggaagatc aggatttat aagccacgg gccggggat 
      ttacccatt agggagggg aaagggccg gttgggagt 
      cgcggggcg aggattgtt tgggccctg gatggcggg 
      tttatcgtc gttacggga tttgataaa gtctccgag 
      agacaaatc atggttttc aaagccact gatttgtca 
      aatccctta aatacgatt ggtctggac gtgtccccg 
      gctatcttg attccgttt tacgatgag gacagctcg 
      actttgttc gtgacgggg aagggcgat ttgacgatt 
      tatgcgttc gaaattacg gaggaatcg ccctatata 
      tgcccctta agccaccac aggtgcaat aatctacac 
      caaggtctt tcatttttg ccaaaaaat gtgtgtgat 
      gtcagtaat gtcgaattt gcgaaagcg ctaagactc 
      actaataat acgatagag ccgttgtct ttcaccgtt 
      ccaaggatt aaaacggaa ctcttccaa gacgacttg 
      ttccccccg accaaggtc acgtggaaa ccaagtttg 
      tcaagtaca gagtggtac aataacaaa gataaaacc 
      ccacaaagg atcagtctc aagccagaa ggcatggat 
      ttgttatcc gagtctcaa ggccagact aatcctgaa 
      aggaccata aacaagtct gcttccact cctttcatt 
      ggagcctct cagccttat aacaggtta gcgtggaac 
      gccgatttg aacgaaaaa actaaaatg aaacaagag 
      gagttgcag aaatctgtg agtaacagg atggaggtc 
      aacttgaag ttggaacag gacgagatg gaaggcgtt 
      gatgaaaaa gaatggacc gattaattt gacttgact 
      ctctagccg ccttaatca tgacatact ttttaattt 
      aattcctag tatcgctaa gatataatc atcgcttaa 
      tttcgatat tacgcaatt tacacaagt gattttttt 
      atattttta tagatttta cgctttgat atttttcta 
      cagttatta tgacatcta gtcagattt ttctatgtg 
      atctctgat agttctcca aacattccc gactctgca 
      tttaatctg ccatagcgg tgccaaaat taaacgacc 
      aaatttctt gctcactcc cggtaacca atcgctcaa 
      aacaatttt ttagtcatt taagtgcta taatttatt 
      tcgtattgt gtaatttag tgttgtgac agtgtagga 
      tgtagttca tgtaaggtt ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY