Status
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Gene information
General information
TC008018 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3950146..3993240
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008018
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09882

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008018 RA
      MASCPFRRA SSHALPNGA LKRSFTSRQ SSIQIPNDD 
      QDDEDKNTL NLKHLNDAI VTLTAPPNE ALQVALESL 
      LLKENETNP SRYSCLNRL PSDVSHCSH YAYLEDLYD 
      IISSEDEID EFEFLDKLG QELIVTSCT GRLERALKC 
      LGSDLKEFL TTLDGVHDV LKYQEEVEH EGEAFICTS 
      DEDHLRLDF TTERPAVAY LLVGSLKAI ARILYNTQA 
      YVEVAQNRS DSRNFSFKI RHVGGEVLE TPISTPTPD 
      VDLQMGVAT FCKAFPWHF VLDRKLDLV QLGSGFMRL 
      FGRLLGNLG KSVATYFEF HRPRSITLS FGEIIKRAN 
      TPFVLAIRN LPGVESFPA EGLELKGQM VYCPESDSI 
      LFIGSPFLD GLEGLTGSG LFISDIPMH DATRDVILV 
      GEQARAQDG LRRRMDKLK SSIEEGNRA VDKEREKNV 
      SLLHLIFPP DIAKRLWLG QTIEAKTHE DVTMLFSDI 
      VGFTSICST TTPMMVINM LQDLYNQFD VFCGQLDVY 
      KVETIGDAY CVAGGLHKE TDTHALQIA WMALKMIDT 
      CSHHHTHQG QPIRMRIGL HTGSVLAGV VGVKMPRYC 
      MFGHNVTIA NKFESGSEP LRINVSPTT YERLAAFEG 
      FRLEARERS CLPKDFPAN IPGTCHFLS DYKHPALPP 
      DAALTQHIE AGLKEIGLS SETS
    CDS sequence
    • >TC008018 RA
      atggcgtct tgtcctttc cggcgagcg tcctcgcac 
      gctctcccc aacggggcc ctgaagaga tctttcacg 
      tcgagacag tcctccatc caaatcccc aacgacgac 
      caagatgac gaagacaaa aatacgctc aacttgaag 
      catctgaac gatgccatc gtgacgctg acggcgccg 
      ccgaacgaa gcacttcaa gtcgccttg gagtctctc 
      ctgctgaag gaaaatgaa actaatccc agtaggtac 
      tcctgtcta aaccgatta ccgagcgac gtcagccat 
      tgtagccat tatgcctat ttggaggac ttatacgac 
      atcatttcc tccgaggac gaaatcgac gaatttgaa 
      tttctggac aagctgggc caggaactg atcgtaaca 
      tcatgcact ggacgcttg gaacgagcc ctcaaatgc 
      ctcgggagc gacctgaag gagttcctc accactctg 
      gacggcgtc cacgacgtg ctcaagtac caggaggag 
      gtggagcac gaaggcgaa gccttcatt tgcacgagt 
      gacgaagac cacctccgg ctcgacttc accacggag 
      aggcccgcc gtggcctac ctgctagtc ggtagcctc 
      aaggccata gcccggatt ctctacaac acccaagcc 
      tacgtagaa gtcgcccag aaccggagc gactcccgc 
      aatttcagc ttcaagatc cggcacgtc gggggcgag 
      gtcctggag acccccatc tcgacgccc acgcccgac 
      gtggacctc cagatgggc gtggccacc ttctgcaag 
      gccttcccg tggcacttt gtcctcgac cggaagctg 
      gacttggtg cagctcggt tccgggttc atgcggctt 
      ttcgggcga ctgttgggc aatctgggc aagtccgtg 
      gcgacctac ttcgagttc caccgcccc agaagtatt 
      actttgagc ttcggcgag atcattaaa agggcgaat 
      acgccgttc gtgctggcc ataaggaac cttccggga 
      gttgaatcg tttccagcg gaagggctg gagctgaag 
      ggccagatg gtgtattgc ccggaatcc gactcgatc 
      ctcttcatc ggctccccg ttcctcgac ggcctcgag 
      gggctgacc ggaagcggc ctcttcatc tcggacatt 
      cccatgcat gatgccacc agagatgtc atcctcgta 
      ggcgaacag gcccgagcg caggacggc ttacgccga 
      cgaatggat aaactcaag agctcgatc gaggagggc 
      aacagggcc gtcgataag gaacgagaa aagaacgtc 
      agcttattg catctgatt ttcccgcca gacatcgcc 
      aagcggctc tggctaggc cagaccatc gaggcgaaa 
      acccacgaa gacgtcacc atgctcttc agcgacatc 
      gttggcttc acgtccatc tgctccacg accactccc 
      atgatggtc attaatatg ctgcaggat ttgtacaat 
      caattcgac gtgttttgc ggccaactg gacgtctac 
      aaagtggag acaataggg gacgcctat tgcgtggcc 
      ggaggcctc cacaaagag accgacacc cacgccctc 
      cagatcgcc tggatggcc ctcaagatg atcgacact 
      tgctcgcac catcacacg caccaaggc cagccgata 
      aggatgcgg atcggcctg cacacgggg tcggtgctg 
      gcgggggtg gtgggggtg aaaatgccg cgctattgc 
      atgtttggc cacaacgtg accatcgcc aacaagttc 
      gaatccggg agcgagccg ctccggata aacgtcagc 
      ccgaccaca tacgagagg ctggcggcg ttcgagggc 
      ttcaggctg gaggcgcgc gagcgctcc tgcctcccc 
      aaggacttc cccgccaac attccgggc acttgccac 
      ttcctttcc gactacaag cacccggcc ttgccgccg 
      gacgccgcc ctcactcag cacatcgaa gccggcttg 
      aaagagatc ggactgagc tccgagact tcctaa
    mRNA sequence
    • >TC008018 RA
      acgtccaaa acgcgacga taagcacgt gatcatacc 
      gactgatag cggagggaa aaatttata atccgaata 
      aataatcgg cgatgaaac acaaatgag tcgatagag 
      tcgtaacga tggcgtctt gtcctttcc ggcgagcgt 
      cctcgcacg ctctcccca acggggccc tgaagagat 
      ctttcacgt cgagacagt cctccatcc aaatcccca 
      acgacgacc aagatgacg aagacaaaa atacgctca 
      acttgaagc atctgaacg atgccatcg tgacgctga 
      cggcgccgc cgaacgaag cacttcaag tcgccttgg 
      agtctctcc tgctgaagg aaaatgaaa ctaatccca 
      gtaggtact cctgtctaa accgattac cgagcgacg 
      tcagccatt gtagccatt atgcctatt tggaggact 
      tatacgaca tcatttcct ccgaggacg aaatcgacg 
      aatttgaat ttctggaca agctgggcc aggaactga 
      tcgtaacat catgcactg gacgcttgg aacgagccc 
      tcaaatgcc tcgggagcg acctgaagg agttcctca 
      ccactctgg acggcgtcc acgacgtgc tcaagtacc 
      aggaggagg tggagcacg aaggcgaag ccttcattt 
      gcacgagtg acgaagacc acctccggc tcgacttca 
      ccacggaga ggcccgccg tggcctacc tgctagtcg 
      gtagcctca aggccatag cccggattc tctacaaca 
      cccaagcct acgtagaag tcgcccaga accggagcg 
      actcccgca atttcagct tcaagatcc ggcacgtcg 
      ggggcgagg tcctggaga cccccatct cgacgccca 
      cgcccgacg tggacctcc agatgggcg tggccacct 
      tctgcaagg ccttcccgt ggcactttg tcctcgacc 
      ggaagctgg acttggtgc agctcggtt ccgggttca 
      tgcggcttt tcgggcgac tgttgggca atctgggca 
      agtccgtgg cgacctact tcgagttcc accgcccca 
      gaagtatta ctttgagct tcggcgaga tcattaaaa 
      gggcgaata cgccgttcg tgctggcca taaggaacc 
      ttccgggag ttgaatcgt ttccagcgg aagggctgg 
      agctgaagg gccagatgg tgtattgcc cggaatccg 
      actcgatcc tcttcatcg gctccccgt tcctcgacg 
      gcctcgagg ggctgaccg gaagcggcc tcttcatct 
      cggacattc ccatgcatg atgccacca gagatgtca 
      tcctcgtag gcgaacagg cccgagcgc aggacggct 
      tacgccgac gaatggata aactcaaga gctcgatcg 
      aggagggca acagggccg tcgataagg aacgagaaa 
      agaacgtca gcttattgc atctgattt tcccgccag 
      acatcgcca agcggctct ggctaggcc agaccatcg 
      aggcgaaaa cccacgaag acgtcacca tgctcttca 
      gcgacatcg ttggcttca cgtccatct gctccacga 
      ccactccca tgatggtca ttaatatgc tgcaggatt 
      tgtacaatc aattcgacg tgttttgcg gccaactgg 
      acgtctaca aagtggaga caatagggg acgcctatt 
      gcgtggccg gaggcctcc acaaagaga ccgacaccc 
      acgccctcc agatcgcct ggatggccc tcaagatga 
      tcgacactt gctcgcacc atcacacgc accaaggcc 
      agccgataa ggatgcgga tcggcctgc acacggggt 
      cggtgctgg cgggggtgg tgggggtga aaatgccgc 
      gctattgca tgtttggcc acaacgtga ccatcgcca 
      acaagttcg aatccggga gcgagccgc tccggataa 
      acgtcagcc cgaccacat acgagaggc tggcggcgt 
      tcgagggct tcaggctgg aggcgcgcg agcgctcct 
      gcctcccca aggacttcc ccgccaaca ttccgggca 
      cttgccact tcctttccg actacaagc acccggcct 
      tgccgccgg acgccgccc tcactcagc acatcgaag 
      ccggcttga aagagatcg gactgagct ccgagactt 
      cctaataac gccggagca atagcccgc ttgtaccat 
      agcgcaata atcgcacac tgtgtatat accgctgga 
      ccagacgac gcaataagt cacgatttc tgttctcac 
      cgcttttgt acatatgta aagacggga cgagctcac 
      ttttcaccg actttgtgg attcgatgt atgtgttac 
      tactgtgtt caacttcgg actcgtgcc cgggactgc 
      atgtataaa gaaaaccat ttctattta cctgaaagt 
      ataaagcgt ttgttctaa acttcggag ataggaatt 
      tgtaagatt gtagattga gtttcaaac aaaactgtc 
      tttccttct tgagcttta ttcgatcaa atcaaagac 
      tattctcat gttaatgta aagtagacg taactatta 
      tttctcatt gttttacgt tgagttgga agattgtgt 
      tgcattgaa taaattatt aaaattacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY