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Gene information
General information
TC007992 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3542140..3548183
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007992
    iBeetle screen iB_09878

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007992 RA
      MDDSNTKKI ESLGKMTAM SQEEIVSAV RTVAQGLEA 
      LRSEHAGIL HGLHEAPDP VANERAGLV QQSAEMIEL 
      GLGEAQVIM ALANHLQLI EAEKQKLRT QVRRLCQEN 
      AWLRDELAS TQQRLQASE QTVAQLEEE KRHLEFMSS 
      VSKYDQDVN DDNTSEHSR SEKPDPVVD LFPDDDNED 
      RNNMSPTPP NQLQLSQQV NAGYEIPAR LRTLHNLVI 
      QYASQGRYE VAVPLCKQA LEDLEKTSG HDHPDVATM 
      LNILALVYR DQNKYKEAA NLLNDALAI REKTLGENH 
      PAVAATLNN LAVLYGKRG KYKEAEPLC KRALDIREK 
      VLGRDHPDV AKQLNNLAL LCQNQGKYE EVEKYYQRA 
      LEIYEKRLG PDDPNVSKT MNNLASCYL KQGKYKEAE 
      VLYKQILNR AHEREFGTI DGDNKPIWQ VAEEREENK 
      AKNRENAPY GEYGGWHKA AKVDSPTVT TTLKNLGAL 
      YRRQGKYEA AETLEDCAL RSRKEALDI VKHSKVANI 
      LGDMDRRKA GSVKDKTSR RGSRESLDM SYDGEEPNT 
      ARTPDMEKG GLKTKLFNA LGINSPVNK PPSS
    CDS sequence
    • >TC007992 RA
      atggatgat agcaacact aagaaaatt gaaagtttg 
      ggaaaaatg acggcgatg tcgcaggaa gagatcgtc 
      tcagctgtg cgaacggtg gctcagggg ttggaggcg 
      ctccgctca gagcacgcc ggcatcctc catggcctc 
      cacgaagcc cccgatccg gtggccaat gaacgcgcc 
      ggtctggtg caacaaagt gcagaaatg atcgaattg 
      ggactggga gaagcgcaa gtgataatg gccttggcc 
      aaccacctc caattaatc gaggccgaa aaacaaaaa 
      ctgcgaact caagtgcgt cgcttatgc caagaaaac 
      gcatggtta agagacgaa ttggcctcc acccaacaa 
      agactccaa gcaagcgaa cagaccgtg gcccaatta 
      gaagaagaa aaacgtcat ttggaattt atgagctcc 
      gttagtaaa tacgatcag gacgtcaac gacgacaac 
      actagcgag cactcacga agcgaaaaa ccagatccg 
      gtcgtcgat cttttccca gacgatgat aacgaggat 
      cgaaataat atgtcacca acgccaccc aatcaattg 
      caactttca caacaagtg aatgccgga tatgaaatt 
      ccggcacga cttcgcacg ctgcataac ttggtgatt 
      caatacgcc tctcaagga cggtacgaa gtggctgtg 
      ccgttatgc aagcaggca ctggaggat ttggaaaaa 
      acaagcgga catgaccat ccagacgtc gccacgatg 
      ctgaatatc ctagctttg gtctatcgg gatcaaaat 
      aagtacaaa gaggcggcg aatttgtta aacgatgct 
      ttggcaatt cgcgagaaa actttgggg gagaaccat 
      ccggccgtc gctgccact cttaacaat ttggctgtt 
      ttgtacggg aaaaggggc aagtacaag gaggccgag 
      cctctgtgc aaacgcgcc ttagacata cgagagaaa 
      gtgctaggg cgcgaccat cccgacgtc gccaaacag 
      ctgaacaac ctcgctttg ctctgccag aatcagggc 
      aaatacgag gaggtggag aagtattac cagcgcgcg 
      ctggagatt tacgagaag cggctgggc cccgacgac 
      cccaacgtc agcaaaaca atgaacaat ctggcttct 
      tgctatttg aagcagggc aagtacaaa gaagccgaa 
      gtcctatac aagcaaatc ctgaaccgg gcacacgag 
      cgcgagttc ggaacaatc gatggtgat aacaaaccg 
      atttggcaa gtggcggag gaacgcgaa gagaataaa 
      gccaagaac agggagaac gctccatat ggggagtat 
      ggtgggtgg cataaggcg gccaaagtt gattcgcca 
      acggtgacg accactttg aaaaatcta ggggcgctg 
      taccgcaga caggggaag tacgaagct gcggaaact 
      ttggaggat tgtgcgttg cgaagcagg aaagaggct 
      ttggacatc gtgaaacac tccaaagtg gccaacatc 
      cttggcgat atggaccgc cgtaaggct gggagcgta 
      aaagacaaa acctcgcgt cgtggttcc cgggaatcg 
      cttgacatg tcgtacgac ggtgaggag ccaaacact 
      gcaaggacc cccgacatg gaaaagggc ggattgaaa 
      acgaaactg tttaacgcc ctgggcatt aattcaccc 
      gtcaacaag ccgcctagc tcctaa
    mRNA sequence
    • >TC007992 RA
      acactgaga gaaaaagga atgagaaga gagttatca 
      aaagcgcag ccattaatg tcagaagaa agtgattgc 
      agtgtcaaa aatagccca atttgagtg ttttaacgc 
      ttggctgcc aaataaagg ttacgctat ggtctactg 
      attgccata gcaggtgag cagtgcatt cccgtggca 
      tggatgata gcaacacta agaaaattg aaagtttgg 
      gaaaaatga cggcgatgt cgcaggaag agatcgtct 
      cagctgtgc gaacggtgg ctcaggggt tggaggcgc 
      tccgctcag agcacgccg gcatcctcc atggcctcc 
      acgaagccc ccgatccgg tggccaatg aacgcgccg 
      gtctggtgc aacaaagtg cagaaatga tcgaattgg 
      gactgggag aagcgcaag tgataatgg ccttggcca 
      accacctcc aattaatcg aggccgaaa aacaaaaac 
      tgcgaactc aagtgcgtc gcttatgcc aagaaaacg 
      catggttaa gagacgaat tggcctcca cccaacaaa 
      gactccaag caagcgaac agaccgtgg cccaattag 
      aagaagaaa aacgtcatt tggaattta tgagctccg 
      ttagtaaat acgatcagg acgtcaacg acgacaaca 
      ctagcgagc actcacgaa gcgaaaaac cagatccgg 
      tcgtcgatc ttttcccag acgatgata acgaggatc 
      gaaataata tgtcaccaa cgccaccca atcaattgc 
      aactttcac aacaagtga atgccggat atgaaattc 
      cggcacgac ttcgcacgc tgcataact tggtgattc 
      aatacgcct ctcaaggac ggtacgaag tggctgtgc 
      cgttatgca agcaggcac tggaggatt tggaaaaaa 
      caagcggac atgaccatc cagacgtcg ccacgatgc 
      tgaatatcc tagctttgg tctatcggg atcaaaata 
      agtacaaag aggcggcga atttgttaa acgatgctt 
      tggcaattc gcgagaaaa ctttggggg agaaccatc 
      cggccgtcg ctgccactc ttaacaatt tggctgttt 
      tgtacggga aaaggggca agtacaagg aggccgagc 
      ctctgtgca aacgcgcct tagacatac gagagaaag 
      tgctagggc gcgaccatc ccgacgtcg ccaaacagc 
      tgaacaacc tcgctttgc tctgccaga atcagggca 
      aatacgagg aggtggaga agtattacc agcgcgcgc 
      tggagattt acgagaagc ggctgggcc ccgacgacc 
      ccaacgtca gcaaaacaa tgaacaatc tggcttctt 
      gctatttga agcagggca agtacaaag aagccgaag 
      tcctataca agcaaatcc tgaaccggg cacacgagc 
      gcgagttcg gaacaatcg atggtgata acaaaccga 
      tttggcaag tggcggagg aacgcgaag agaataaag 
      ccaagaaca gggagaacg ctccatatg gggagtatg 
      gtgggtggc ataaggcgg ccaaagttg attcgccaa 
      cggtgacga ccactttga aaaatctag gggcgctgt 
      accgcagac aggggaagt acgaagctg cggaaactt 
      tggaggatt gtgcgttgc gaagcagga aagaggctt 
      tggacatcg tgaaacact ccaaagtgg ccaacatcc 
      ttggcgata tggaccgcc gtaaggctg ggagcgtaa 
      aagacaaaa cctcgcgtc gtggttccc gggaatcgc 
      ttgacatgt cgtacgacg gtgaggagc caaacactg 
      caaggaccc ccgacatgg aaaagggcg gattgaaaa 
      cgaaactgt ttaacgccc tgggcatta attcacccg 
      tcaacaagc cgcctagct cctaaacat ctccgatac 
      attttaagc tttgccgaa attttaaat aacattcca 
      ccgatttat ctaagcctt ttctagagt tattaccgt 
      gcatggcgc agtgtctag gcaagtaca agcttaaaa 
      tgtcaaact tactgtaga taaataact taaaatgaa 
      atgaaaaag tatttaatt attaattga tagtattat 
      tttattctt ttttgttac gcattattt tttaattgt 
      aacgaaagt gataaaagt gggtggtct tattgtaca 
      cgacgcttc ctcaggtac taatgcact gggttgacg 
      atttttaat tgcaaaatc cgcctaata aatacgtaa 
      agcattttt tgtcttccc ttcacgttc ctgattttc 
      ggatcttcc agaatttgt caattatta cgttagtga 
      atctctttt tgtgaaatt cttttttgt ataattttg 
      cacatagtt tattgtact ataaaacga gctaaattg 
      tgttaattt aattgaatt ctagtgttt gttaacatt 
      gtttataaa ataaattca tatgtttat c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY