Status
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Gene information
General information
TC031101
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007990,TC007991
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3535528..3538039
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031101
    iBeetle screen iB_09877

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031101 RA
      MGRPRKTAR QRFKAPAGS KWPPTFKLG WFTAGKICL 
      EEVAINDVV LRVPLKVHT GREPKRKRG RRPLLGKRT 
      EKSATKSIV KRDTQRPRE RAIKVLRKR RRRPLLKKR 
      LVQSVGRRR RPKQVQEFP VNGKKDLIV SEVGILDWK 
      QQPVGLRRL VTNLKSSER RNSESVFRN GERRTYLPA 
      VKKLGSAKL KNVEFSFSE LAHSLDQIK FPSFDWKVS 
      MISNRDVQT ELRFYNTVK PKSVSFSQQ NLDFKIAID 
      KVPVALLGC PETVESQRD IEILLEIVN RLGLHDCMV 
      AKILIPPTL ILGLTIFSY YSYVLHFCV HIIDSVIKK 
      TSYLFVYHV TILMFLWSF LATALKKHH SIPDEYRLS 
      LSEHSRLLN YTEDEANAI LKKLVRLRN LELYTCGPH 
      GRPRYCKTC MLIKPDRAH HCTNCQRCI LKMDHHCPW 
      VDNCIGFSN YKQFILMLF YTTLWCAFY AGTVAEYII 
      DLWKDIHTN VSKLIVGIG FLCAAFLGM VILFLFVYH 
      LKLVFKNET TLEALRDTT YYQDNTTFD LGQWSNFTE 
      VFGDNVCCW LFPVTSGKG NGYEFRVNS LI
    CDS sequence
    • >TC031101 RA
      atgggtaga ccaagaaaa accgcacga cagcggttc 
      aaggcgccg gctgggagc aaatggcct cctacgttc 
      aagctgggg tggtttaca gccggtaaa atttgtctg 
      gaggaggtg gcaatcaac gacgttgtt ttgagggtg 
      cccctgaag gtccacact ggtcgagaa ccgaagaga 
      aagcggggg cggcggccg ctgcttggg aaacgtaca 
      gaaaaaagt gctacaaag tcgattgtt aaaagagat 
      actcaaaga ccgcgcgaa agagctata aaggtgctc 
      cggaaacga aggcggcga cccctgctt aagaaacgc 
      ctagtacaa agtgttgga cgaagaagg cggccaaag 
      caggttcag gaatttcct gttaatgga aagaaagat 
      ctcatagtc agcgaagtt ggtattctt gactggaag 
      cagcaacct gtgggttta aggcgtttg gtgacgaat 
      ctcaagtct agtgaaagg agaaatagt gagtctgtg 
      tttcgaaac ggggaaaga cgaacgtat ttgccagcg 
      gttaaaaaa ctgggcagt gcaaagttg aaaaatgtg 
      gagtttagt tttagtgaa ctggctcat agtttggat 
      caaattaag tttccgtcg tttgattgg aaagtctca 
      atgattagc aatcgcgac gtccagact gagcttaga 
      ttttacaat acagtgaaa ccaaaaagt gttagtttt 
      tcccaacaa aacctcgac tttaagatt gcaatagat 
      aaagtaccc gttgcgtta ctaggctgc cctgaaact 
      gttgaatct caaagagat atcgaaatt ttattagaa 
      atcgttaat agattaggc ctacatgac tgcatggtt 
      gcaaagatc ctaatcccg ccaaccctc attttaggt 
      ctaacaatt ttttcctac tactcgtac gtcctccac 
      ttttgcgtc cacatcata gacagtgta atcaaaaaa 
      acaagttac ctcttcgtc tatcacgtg actatcctc 
      atgttcctg tggtctttc cttgcaacg gcgctcaaa 
      aaacaccac tcgatccca gacgagtac cgcctgagt 
      ctgtccgaa cattcccga cttctcaac tacaccgaa 
      gacgaagct aacgctatt ttgaaaaag ttggtgcgc 
      ttgcgcaat ctagaactg tacacttgt ggcccccac 
      ggccgacct cgttactgc aagacttgt atgcttatc 
      aagcccgac agagcccat cattgtaca aattgccag 
      agatgtatt ctcaagatg gaccatcat tgcccatgg 
      gtggataac tgtattggg tttagtaat tacaaacag 
      ttcattttg atgctgttt tacacaact ctatggtgc 
      gctttttat gctggaacc gtggctgaa tacattata 
      gatctctgg aaagacatt cacacaaat gtcagcaag 
      ctcattgtc ggaatcgga tttttatgt gcggcattt 
      ttggggatg gtgattttg ttcttgttt gtttatcac 
      ttgaagtta gtttttaaa aacgagact acgcttgaa 
      gcgctacgt gatacaaca tactatcaa gacaatacg 
      acttttgac ttgggtcaa tggtcgaat tttacggaa 
      gttttcggc gataatgtg tgttgttgg ttgtttccg 
      gtgacaagt gggaaagga aatggttac gagtttcgg 
      gtgaacagc ctgatttaa
    mRNA sequence
    • >TC031101 RA
      tgacaaaaa atacttaaa cagaattaa caaatacgc 
      aaaaatcca cttttgata atcattata tcaataata 
      catccgaga gacaaacca aatttgcaa atggcgcgt 
      catctggcg gaagcttgg tggcactag tgcaaattt 
      ccaatcgcg ggaatgctt tttgtggaa gtaatcata 
      ttaaaacta atagttact cagccacac tttgcgtct 
      tttctcaaa taaaaaggt tatgtggtt tatttacta 
      aaattaaac catgggtag accaagaaa aaccgcacg 
      acagcggtt caaggcgcc ggctgggag caaatggcc 
      tcctacgtt caagctggg gtggtttac agccggtaa 
      aatttgtct ggaggaggt ggcaatcaa cgacgttgt 
      tttgagggt gcccctgaa ggtccacac tggtcgaga 
      accgaagag aaagcgggg gcggcggcc gctgcttgg 
      gaaacgtac agaaaaaag tgctacaaa gtcgattgt 
      taaaagaga tactcaaag accgcgcga aagagctat 
      aaaggtgct ccggaaacg aaggcggcg acccctgct 
      taagaaacg cctagtaca aagtgttgg acgaagaag 
      gcggccaaa gcaggttca ggaatttcc tgttaatgg 
      aaagaaaga tctcatagt cagcgaagt tggtattct 
      tgactggaa gcagcaacc tgtgggttt aaggcgttt 
      ggtgacgaa tctcaagtc tagtgaaag gagaaatag 
      tgagtctgt gtttcgaaa cggggaaag acgaacgta 
      tttgccagc ggttaaaaa actgggcag tgcaaagtt 
      gaaaaatgt ggagtttag ttttagtga actggctca 
      tagtttgga tcaaattaa gtttccgtc gtttgattg 
      gaaagtctc aatgattag caatcgcga cgtccagac 
      tgagcttag attttacaa tacagtgaa accaaaaag 
      tgttagttt ttcccaaca aaacctcga ctttaagat 
      tgcaataga taaagtacc cgttgcgtt actaggctg 
      ccctgaaac tgttgaatc tcaaagaga tatcgaaat 
      tttattaga aatcgttaa tagattagg cctacatga 
      ctgcatggt tgcaaagat cctaatccc gccaaccct 
      cattttagg tctaacaat tttttccta ctactcgta 
      cgtcctcca cttttgcgt ccacatcat agacagtgt 
      aatcaaaaa aacaagtta cctcttcgt ctatcacgt 
      gactatcct catgttcct gtggtcttt ccttgcaac 
      ggcgctcaa aaaacacca ctcgatccc agacgagta 
      ccgcctgag tctgtccga acattcccg acttctcaa 
      ctacaccga agacgaagc taacgctat tttgaaaaa 
      gttggtgcg cttgcgcaa tctagaact gtacacttg 
      tggccccca cggccgacc tcgttactg caagacttg 
      tatgcttat caagcccga cagagccca tcattgtac 
      aaattgcca gagatgtat tctcaagat ggaccatca 
      ttgcccatg ggtggataa ctgtattgg gtttagtaa 
      ttacaaaca gttcatttt gatgctgtt ttacacaac 
      tctatggtg cgcttttta tgctggaac cgtggctga 
      atacattat agatctctg gaaagacat tcacacaaa 
      tgtcagcaa gctcattgt cggaatcgg atttttatg 
      tgcggcatt tttggggat ggtgatttt gttcttgtt 
      tgtttatca cttgaagtt agtttttaa aaacgagac 
      tacgcttga agcgctacg tgatacaac atactatca 
      agacaatac gacttttga cttgggtca atggtcgaa 
      ttttacgga agttttcgg cgataatgt gtgttgttg 
      gttgtttcc ggtgacaag tgggaaagg aaatggtta 
      cgagtttcg ggtgaacag cctgattta aagaagtgg 
      cggtgatgc tttaaattt tattcacat ttaaacaat 
      aataaaatc gtaaaaccc acggataca gctcgatat 
      ttgacatgg gaaatatgg aaacctatc tatataact 
      ataatagta attataata aaatccttt atataacat 
      ctcatataa tattgtgaa aagaaaaac acttgaatg 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY