Status
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Gene information
General information
TC007971 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3297640..3301434
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007971
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09875

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007971 RA
      MAAMSVIGI DLGNESCYV AVAKAGGIE TIANDYSLR 
      ATPSFIAFS DKNRILGVA AKNQQITNM KNTVYGLKR 
      LIGRKYRDP HVQRELQML PFNVIEVAQ GNIGIKVNY 
      LNEEHIFSP EQCLAMLLT KLKDISSTA LQTPINDCV 
      ISVPSYFTN NERKALMDS AAIAGLNVL RLFNETTAT 
      ALSYGIYKQ DLPGPDDKS RNVVFVDCG HSSLQVFAC 
      AFNKDKLRM IATASDPYL GGRDFDLAL ADHFCKEFQ 
      TKYHIDAKS NARAFSRLL GEVEKVKKQ MSANSTSLP 
      LNIECFMDD KDVHSSIKR TDMEQLCAP LFQRVETTL 
      KQCLDASGL KLDEIYSVE IVGGSSRIP AIKQLIEKV 
      FKKVPSTTL NQDEAVSRG CALQCAMLS PAVRVREFG 
      VKDVQNYAV SVSWDASTD GEAAGEVEA FPVNHQVPY 
      SKMLTFYRQ EPFSIKAMY SGNVPYPDK NIGTWIVKD 
      IRPNAEGKP QKVKVKVRI NLHGIMTVS SASLFEAKE 
      SSEAENEET QKVQQQEEQ EKPTNQNST DQQNDVDAP 
      MADGVNNAA PEDGDKDKK KKQVLKSIE LPIESLTAG 
      FSQVEINQY TEQEFKMIA ADRQEKERA DARNAFEEY 
      VYELRGKIS SDDELGAFI LENDRSALM QQLDDMENW 
      LYEDGAECN RQVYQDKLA ELKSKGEPI QTRKVEFEL 
      RPHVIEDFA KSLQLTMKA LEAIKGNDP KFAHLTEDE 
      VKKVDNAFK NSFQWLEQT RAKLVNAPK HVAPPVTVS 
      QIRQEKNDF ECTVSPILN KPPPKAPSP PKEEKSPTD 
      EKAQNAQTE QNQQNQQNQ NQQENMDWS SAN
    CDS sequence
    • >TC007971 RA
      atggcggca atgtcagtc ataggaatt gacctgggg 
      aacgagtct tgctatgtc gccgtcgcc aaagccggt 
      ggcatcgag accatcgcc aacgattac agtttacga 
      gcgactcca tcattcatc gccttttcg gacaaaaac 
      cgaatcttg ggggtcgcc gccaaaaac caacaaatc 
      accaacatg aaaaacacg gtttatgga ctcaaaagg 
      ctcattggg cggaagtat agggacccc cacgtgcag 
      cgggagcta caaatgttg ccttttaac gttatagaa 
      gtagcacaa ggaaatatc ggtattaag gttaactat 
      ttaaatgag gaacacata ttctcgccg gaacagtgt 
      ctagctatg ttactaacc aaattaaag gatatttcg 
      tcgactgct ctacaaacc cctattaac gactgtgtt 
      atctcagtt ccctcatac tttacaaat aatgagaga 
      aaagcactc atggattct gcggctatt gctggtttg 
      aatgtactc agattattc aacgagacg accgctacg 
      gccttgtct tacggcatt tacaagcag gacttgccg 
      gggcccgat gacaagtct cgtaatgtt gttttcgtt 
      gattgcggt cattcctct cttcaagtt ttcgcttgc 
      gcgttcaat aaagacaaa cttcgcatg attgcgacc 
      gcttctgat ccatacctc ggcggtaga gacttcgac 
      cttgctttg gccgaccat ttctgcaaa gaattccaa 
      acgaaatat cacatcgac gctaaatct aacgctcgc 
      gccttcagt cgccttctc ggcgaagtg gaaaaagtc 
      aaaaagcag atgtcggcc aactccacg tcattaccc 
      ctcaatatc gagtgtttc atggacgat aaggacgta 
      cacagcagc ataaagcga accgatatg gagcagttg 
      tgtgctcct ttgtttcag agagtcgaa acgacctta 
      aaacaatgc ctggacgcg tcaggctta aaattagac 
      gaaatttac tcagtcgag atagtcggc gggtcgagc 
      aggattcct gctatcaaa caattgata gagaaggtt 
      tttaaaaaa gtgccaagc actactctg aatcaggac 
      gaggctgta agcaggggt tgcgctcta cagtgcgct 
      atgctttca ccggcggtc cgggttagg gaatttggc 
      gttaaagac gtacagaat tatgcagtt tcggtgtca 
      tgggatgcc agcactgat ggggaagcc gcaggggag 
      gttgaggcc ttcccggtc aaccaccag gtcccctat 
      tccaaaatg ttgacgttc tacagacaa gaacccttc 
      tccatcaaa gcgatgtat tcgggaaat gtcccctac 
      cctgataaa aacattggc acttggatc gtgaaagac 
      attcgtccg aacgctgag ggcaagccg cagaaggtc 
      aaggtcaaa gtcaggatc aacctgcac ggcatcatg 
      acagtgtcc agtgcgtcg ttgtttgaa gcgaaggag 
      tcaagcgag gctgaaaat gaggaaacg caaaaggtg 
      cagcaacaa gaggagcag gaaaaaccg acgaatcag 
      aattccact gatcagcag aacgacgtt gatgctccg 
      atggctgat ggcgtgaac aatgcggct cctgaggac 
      ggcgataaa gacaaaaag aagaagcaa gtgttgaag 
      agcatcgaa ttaccgatt gaatctctg accgctggc 
      ttctcccaa gtcgaaatc aatcaatac accgagcaa 
      gaattcaaa atgattgct gccgaccgc caggagaag 
      gaacgagcc gatgcaaga aacgcgttt gaagaatac 
      gtttacgaa ttgcgcggg aagatatca tccgacgac 
      gaacttggt gcttttatt ctcgaaaat gatcgttcg 
      gctctaatg caacaatta gacgatatg gaaaattgg 
      ttgtacgaa gatggcgct gagtgcaac cgacaggtt 
      tatcaagac aaattagct gaattaaaa tcaaaagga 
      gagccaatc cagactaga aaagtcgaa ttcgagctg 
      cgaccacac gttattgaa gatttcgcc aaatcgctc 
      caacttacg atgaaagct ttagaggcg attaagggt 
      aacgacccg aaatttgcg catttgact gaagacgag 
      gtcaagaag gtggataat gcgttcaag aattcgttc 
      cagtggttg gagcaaaca agagcgaag cttgtcaac 
      gcacctaag cacgtagcc ccgcctgtt actgtctct 
      cagatccga caagagaag aatgatttt gaatgcaca 
      gtcagtcct attttgaat aagccaccg ccaaaggca 
      ccctcgccc ccgaaagag gaaaagagc ccgacagat 
      gagaaggct caaaatgcg caaactgag cagaaccaa 
      cagaatcag cagaaccag aaccagcag gagaacatg 
      gactggtcg tcggcaaat tag
    mRNA sequence
    • >TC007971 RA
      cagttggca acagttagt ctaaaatca gaaagcacg 
      agctgcgag tgaccgagc gaaaaaacc ggtaccttc 
      caactccca gtgcaaata atcgcaaaa tttgtgtta 
      ttcccactc gaagagagc cccacacag tgtttttgt 
      gtttaatta tcgccattt gaacatccg gcgtcaaaa 
      taaactcgc attatggcg gcaatgtca gtcatagga 
      attgacctg gggaacgag tcttgctat gtcgccgtc 
      gccaaagcc ggtggcatc gagaccatc gccaacgat 
      tacagttta cgagcgact ccatcattc atcgccttt 
      tcggacaaa aaccgaatc ttgggggtc gccgccaaa 
      aaccaacaa atcaccaac atgaaaaac acggtttat 
      ggactcaaa aggctcatt gggcggaag tatagggac 
      ccccacgtg cagcgggag ctacaaatg ttgcctttt 
      aacgttata gaagtagca caaggaaat atcggtatt 
      aaggttaac tatttaaat gaggaacac atattctcg 
      ccggaacag tgtctagct atgttacta accaaatta 
      aaggatatt tcgtcgact gctctacaa acccctatt 
      aacgactgt gttatctca gttccctca tactttaca 
      aataatgag agaaaagca ctcatggat tctgcggct 
      attgctggt ttgaatgta ctcagatta ttcaacgag 
      acgaccgct acggccttg tcttacggc atttacaag 
      caggacttg ccggggccc gatgacaag tctcgtaat 
      gttgttttc gttgattgc ggtcattcc tctcttcaa 
      gttttcgct tgcgcgttc aataaagac aaacttcgc 
      atgattgcg accgcttct gatccatac ctcggcggt 
      agagacttc gaccttgct ttggccgac catttctgc 
      aaagaattc caaacgaaa tatcacatc gacgctaaa 
      tctaacgct cgcgccttc agtcgcctt ctcggcgaa 
      gtggaaaaa gtcaaaaag cagatgtcg gccaactcc 
      acgtcatta cccctcaat atcgagtgt ttcatggac 
      gataaggac gtacacagc agcataaag cgaaccgat 
      atggagcag ttgtgtgct cctttgttt cagagagtc 
      gaaacgacc ttaaaacaa tgcctggac gcgtcaggc 
      ttaaaatta gacgaaatt tactcagtc gagatagtc 
      ggcgggtcg agcaggatt cctgctatc aaacaattg 
      atagagaag gtttttaaa aaagtgcca agcactact 
      ctgaatcag gacgaggct gtaagcagg ggttgcgct 
      ctacagtgc gctatgctt tcaccggcg gtccgggtt 
      agggaattt ggcgttaaa gacgtacag aattatgca 
      gtttcggtg tcatgggat gccagcact gatggggaa 
      gccgcaggg gaggttgag gccttcccg gtcaaccac 
      caggtcccc tattccaaa atgttgacg ttctacaga 
      caagaaccc ttctccatc aaagcgatg tattcggga 
      aatgtcccc taccctgat aaaaacatt ggcacttgg 
      atcgtgaaa gacattcgt ccgaacgct gagggcaag 
      ccgcagaag gtcaaggtc aaagtcagg atcaacctg 
      cacggcatc atgacagtg tccagtgcg tcgttgttt 
      gaagcgaag gagtcaagc gaggctgaa aatgaggaa 
      acgcaaaag gtgcagcaa caagaggag caggaaaaa 
      ccgacgaat cagaattcc actgatcag cagaacgac 
      gttgatgct ccgatggct gatggcgtg aacaatgcg 
      gctcctgag gacggcgat aaagacaaa aagaagaag 
      caagtgttg aagagcatc gaattaccg attgaatct 
      ctgaccgct ggcttctcc caagtcgaa atcaatcaa 
      tacaccgag caagaattc aaaatgatt gctgccgac 
      cgccaggag aaggaacga gccgatgca agaaacgcg 
      tttgaagaa tacgtttac gaattgcgc gggaagata 
      tcatccgac gacgaactt ggtgctttt attctcgaa 
      aatgatcgt tcggctcta atgcaacaa ttagacgat 
      atggaaaat tggttgtac gaagatggc gctgagtgc 
      aaccgacag gtttatcaa gacaaatta gctgaatta 
      aaatcaaaa ggagagcca atccagact agaaaagtc 
      gaattcgag ctgcgacca cacgttatt gaagatttc 
      gccaaatcg ctccaactt acgatgaaa gctttagag 
      gcgattaag ggtaacgac ccgaaattt gcgcatttg 
      actgaagac gaggtcaag aaggtggat aatgcgttc 
      aagaattcg ttccagtgg ttggagcaa acaagagcg 
      aagcttgtc aacgcacct aagcacgta gccccgcct 
      gttactgtc tctcagatc cgacaagag aagaatgat 
      tttgaatgc acagtcagt cctattttg aataagcca 
      ccgccaaag gcaccctcg cccccgaaa gaggaaaag 
      agcccgaca gatgagaag gctcaaaat gcgcaaact 
      gagcagaac caacagaat cagcagaac cagaaccag 
      caggagaac atggactgg tcgtcggca aattaggtg 
      taattgata tttataatg cttggacac aattttcag 
      agcgaggca cgtcgtaat cgttccctt atactgtat 
      tactagaaa attttctat ttatatttt acttaaacc 
      atcgcagtt tttactaca ttttgttgg aagaaattg 
      tctcaagtc agtttgcac cgatgtgtt ttactgcat 
      tttaaaggg agtttaaat tgtagttct gatcgtagc 
      ttcattcca tttaagtta tttaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY