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Gene information
General information
TC007970 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3292460..3294913
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007970
    iBeetle screen iB_09874

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007970 RA
      MQSSCPPLI SNKLNGAPN ASTNKSKHI VMAERRNES 
      CANKNNDNN YSNIRPIVT PRIPRLPNN WRQSQNRTE 
      ESIYPSVGN VVQFNHFTV GPPLWQPLL PHTYNNLVY 
      PQFVHAVLQ PNLQPCFHR PNQENRRRV HNAANTAGQ 
      TSLNGLRFW EKISPNNAS SGFVFSVMT YNVLAQDLV 
      NQHPYLYAL HRKDSLKWD TRWNNLLAE IRNLNPDIL 
      CLQEVQNTH LDQYFSTLD TLGYQGLYK QRTGPRTDG 
      CAIYYKPHL LTLLEHETV EYNQPTTRL LDRDNVAII 
      AKFASKSRP SHPFVVATT HLLYNPKRQ DVRLAQTQL 
      LLAEIDRIA FNSSTYLPV ILTGDLNST PDSALYKFI 
      SSGFLRRED LALIRSPRS QTGFYGLPP SLRITNECQ 
      HADLLVKRQ ENNVLPRQE ELKLIELRH SGRQMEGAK 
      NKEKVDEKL FNSGGLSHK FFLDSVYKH RNKGDMEAT 
      TFQDMWVSV DYIFFSKKR KHKNGLVLL ERYRLPTSR 
      ELSDLKIPN GELGSDHLS LMARFKLEM
    CDS sequence
    • >TC007970 RA
      atgcagtct agttgcccc cccctaatc agtaacaag 
      ttaaatggg gctccgaat gcgagtact aacaaatcg 
      aagcacatt gtaatggca gaacgtcgc aatgaatcg 
      tgtgccaat aagaacaac gacaataat tacagtaac 
      attcgccct attgtcact cctcgtata ccgaggcta 
      ccgaacaat tggaggcaa agtcagaac cgaaccgag 
      gaaagtatc taccccagt gtcggaaat gtggtgcaa 
      ttcaatcat tttacggtg gggccgccc ttgtggcag 
      cctttacta ccacacacg tacaataat ttagtttat 
      ccgcaattt gtacacgca gtattacaa ccaaattta 
      caaccgtgc tttcacaga cctaaccag gaaaaccgg 
      aggcgggta cacaacgct gctaacact gcggggcaa 
      acgtcactt aacggtttg cgtttctgg gaaaaaatc 
      tcgccaaat aatgccagc tcagggttt gttttctct 
      gtgatgact tacaatgtc ctggcacag gacctcgtc 
      aaccaacac ccttactta tacgctctc caccggaaa 
      gattccctc aaatgggac actcgctgg aataatctc 
      ttggccgaa atacgcaat ttaaaccca gacattttg 
      tgtctccaa gaagtacaa aacacgcat ttagatcaa 
      tatttttca acacttgac actttgggg tatcaaggg 
      ctgtacaag caacgtacg ggcccccgg accgacggc 
      tgcgccatc tactacaag ccccacctc ctcaccctc 
      ctcgagcac gaaacagtt gaatacaac cagcccacc 
      acccgcctc cttgaccgc gacaacgtt gccataatc 
      gcaaaattc gcctctaag tcccgcccc tcgcatccc 
      tttgtcgtc gccaccacc cacctcctc tacaacccc 
      aaacggcaa gacgtacgc ctcgcccaa acgcaactc 
      ctattggcc gaaatcgac cgaattgcc ttcaattcc 
      tctacgtac ctcccggtt atcctcacc ggcgaccta 
      aactccacc cctgactcc gccctctac aaattcata 
      agtagcgga tttttgcgc cgtgaggat ttggctttg 
      attcgttcg ccgagaagt cagaccggg ttctatggc 
      ctaccccca tcgttgcgt ataacaaat gagtgccag 
      catgccgat ttgttagtg aagagacag gagaataat 
      gttctgcca agacaggag gagctcaag ttgattgag 
      ttgaggcat agtgggaga cagatggaa ggggcgaaa 
      aataaggag aaggtcgac gagaagttg ttcaattcg 
      gggggtttg agccataag tttttccta gattcggtg 
      tataagcac aggaataag ggggatatg gaagccacg 
      acctttcag gacatgtgg gtttccgtc gattatatt 
      tttttcagt aagaagcgg aaacataag aatggtctc 
      gtgcttttg gagcgttac agattgcca acgagccga 
      gaattgagc gatctgaag atcccgaat ggggaattg 
      gggtcggat catttgtcg ttgatggcg agatttaag 
      ttggaaatg tga
    mRNA sequence
    • >TC007970 RA
      ttttttaca aaaaaatac aaaatcaca tcgaaacag 
      ctcaaaata cattgatat ttttaactt tgtaatttt 
      ggacttaaa tatcgaatt gttttgggc agttccatg 
      tggtaattt taattgaat aatccttaa cgtcacctg 
      tcaaatgtc cgcaaactt aaccaaaat ttgcaaaaa 
      taattgtga atggacata gggcgcatg cagtctagt 
      tgccccccc ctaatcagt aacaagtta aatggggct 
      ccgaatgcg agtactaac aaatcgaag cacattgta 
      atggcagaa cgtcgcaat gaatcgtgt gccaataag 
      aacaacgac aataattac agtaacatt cgccctatt 
      gtcactcct cgtataccg aggctaccg aacaattgg 
      aggcaaagt cagaaccga accgaggaa agtatctac 
      cccagtgtc ggaaatgtg gtgcaattc aatcatttt 
      acggtgggg ccgcccttg tggcagcct ttactacca 
      cacacgtac aataattta gtttatccg caatttgta 
      cacgcagta ttacaacca aatttacaa ccgtgcttt 
      cacagacct aaccaggaa aaccggagg cgggtacac 
      aacgctgct aacactgcg gggcaaacg tcacttaac 
      ggtttgcgt ttctgggaa aaaatctcg ccaaataat 
      gccagctca gggtttgtt ttctctgtg atgacttac 
      aatgtcctg gcacaggac ctcgtcaac caacaccct 
      tacttatac gctctccac cggaaagat tccctcaaa 
      tgggacact cgctggaat aatctcttg gccgaaata 
      cgcaattta aacccagac attttgtgt ctccaagaa 
      gtacaaaac acgcattta gatcaatat ttttcaaca 
      cttgacact ttggggtat caagggctg tacaagcaa 
      cgtacgggc ccccggacc gacggctgc gccatctac 
      tacaagccc cacctcctc accctcctc gagcacgaa 
      acagttgaa tacaaccag cccaccacc cgcctcctt 
      gaccgcgac aacgttgcc ataatcgca aaattcgcc 
      tctaagtcc cgcccctcg catcccttt gtcgtcgcc 
      accacccac ctcctctac aaccccaaa cggcaagac 
      gtacgcctc gcccaaacg caactccta ttggccgaa 
      atcgaccga attgccttc aattcctct acgtacctc 
      ccggttatc ctcaccggc gacctaaac tccacccct 
      gactccgcc ctctacaaa ttcataagt agcggattt 
      ttgcgccgt gaggatttg gctttgatt cgttcgccg 
      agaagtcag accgggttc tatggccta cccccatcg 
      ttgcgtata acaaatgag tgccagcat gccgatttg 
      ttagtgaag agacaggag aataatgtt ctgccaaga 
      caggaggag ctcaagttg attgagttg aggcatagt 
      gggagacag atggaaggg gcgaaaaat aaggagaag 
      gtcgacgag aagttgttc aattcgggg ggtttgagc 
      cataagttt ttcctagat tcggtgtat aagcacagg 
      aataagggg gatatggaa gccacgacc tttcaggac 
      atgtgggtt tccgtcgat tatattttt ttcagtaag 
      aagcggaaa cataagaat ggtctcgtg cttttggag 
      cgttacaga ttgccaacg agccgagaa ttgagcgat 
      ctgaagatc ccgaatggg gaattgggg tcggatcat 
      ttgtcgttg atggcgaga tttaagttg gaaatgtga 
      ttgtatgtt aaacgttga ctctatttt gtaaaaata 
      aagtttgtt taaggag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY