Status
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Gene information
General information
TC034652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3195011..3197570
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034652
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09871

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034652 RA
      MNSIVLNLQ IACYVLTFL SNLFANWLN SRRYLECLK 
      RLGTLDECL YKFGYQINY KKMNRVFYW SLLLSVGLF 
      LYVALADCV FSVFLNQNI SFINWLSSY VPLTTNYFS 
      SYFIVMSLY FLHVRYSYL SQCFKSYYP TGDLTTLRN 
      VVKMLTDIK DISNSLISQ FALQICALF ILTFLTVTN 
      NLFLTAKGT FHSFQYFIL INIACLHVV ESVSVVLAH 
      YQIHLKLAE ITDLAPKVM SLRTLLPNH SNEIMEFYL 
      LMCNTRLDV QVFGMFTVT PSALLGISA WIGSYLVLT 
      VVFASVLFY TGTESMCRH SYMTFCDNL SDLQNYNVE 
      NWTELVVGT ESHDDSQLQ AVDSDSLSL DRLEKVVTF 
      IIIGQIRDI KTYTFTLDP FFNRLNYLQ LYGNDIRRI 
      RFTTFDTMK LTKLSLVNN KIEYIHAGA FYNCEIKTI 
      DLSHNKLET IETDVFSEE FFYNSTKEI VIRYNEIGA 
      IQSDSFPSS LEILNLDHN NLKVLNADV FDNLDNLQE 
      LTLSHNQLS SLGFVKNLE KLKLLDVSH NKIVALTSQ 
      HFNNLTQLQ VLDLSHNQL AYPQIFQKF NIPERHPPL 
      QISLAFNKL THLVIEENN FRQHIVFLY GNPWNCKCW 
      QMMEKFMLD NKVKRNVCD LKFFGNGDV PYCIEFDKS 
      DCRSGDFGK DSNISQRDI QNFVKVVEG SLDMIRCRL 
      TPRIL
    CDS sequence
    • >TC034652 RA
      atgaactca atcgtgctt aacttacaa attgcctgc 
      tacgttctc acatttctg tcgaattta ttcgcgaat 
      tggttgaac tcgcggaga tatctggag tgtttaaaa 
      cgcctggga acgttggac gagtgtttg tacaagttt 
      ggctaccaa atcaactac aagaagatg aaccgagtg 
      ttttactgg tctttgctt ctctcggtt ggtttattc 
      ttgtatgtt gctctagcc gactgtgtt ttcagcgtg 
      tttctgaac cagaatatt tcgtttatc aattggttg 
      tcttcttac gtgccgctt accactaat tacttctcc 
      tcgtatttt atcgtaatg tctttgtac tttctccac 
      gttagatat tcttatttg agtcagtgt ttcaagagt 
      tattaccct actggtgac ctcacaact ttacgaaac 
      gttgttaaa atgttgact gatattaag gatatcagt 
      aactcgttg atttcgcag tttgctctc caaatctgt 
      gctttattc attttaaca tttctcaca gtgactaac 
      aatctgttc ctaacagct aaaggcaca tttcattcg 
      ttccaatac tttatcctg attaatatc gcgtgcttg 
      catgttgtt gaaagtgtg tcggttgtt ttggcccat 
      tatcagatt cacttaaag cttgcagag ataacagat 
      ttggctccg aaagttatg tctttacga actttgtta 
      ccaaatcac agcaacgaa atcatggaa ttttactta 
      ctaatgtgc aatactaga cttgatgtt caagtgttt 
      ggaatgttt acagtaaca ccatcagca ctgcttggt 
      atatcggca tggattggg tcttatttg gtcctcacc 
      gtcgtcttt gcaagcgtg ctgttttac actgggacc 
      gaatcaatg tgccggcac tcttatatg acgttttgt 
      gataatttg agcgacctt cagaactac aacgttgaa 
      aattggacg gaacttgtg gtaggaaca gagtcacat 
      gatgattcg caacttcag gcagtcgat tccgactcg 
      ctttcatta gacaggttg gaaaaagtg gttactttc 
      attattatc gggcaaatt agagacatc aagacgtac 
      acgttcacc ctcgatccg tttttcaac cgcttgaac 
      tatttacag ttgtatgga aatgatata aggcgcata 
      agatttacg acttttgac acgatgaag ttaacaaag 
      ttaagttta gtgaacaac aagattgag tatattcat 
      gcgggggcg ttctataat tgcgaaatc aaaactata 
      gatttgtcg cacaataag ctggagacg attgaaaca 
      gacgtgttt tcggaagag ttcttctat aattcaacc 
      aaagaaata gtgataaga tataacgaa atcggcgct 
      attcagtcc gatagcttc ccgagcagc ctggagatc 
      ctcaattta gaccacaac aatctaaag gtgttaaat 
      gctgatgtt ttcgacaat ttggataac ttacaagaa 
      ctaacactc agccacaac caattatca tcgttagga 
      ttcgtaaaa aacctcgaa aaactcaaa ctacttgac 
      gtttcgcac aacaaaatt gtcgctctc accagtcaa 
      cacttcaac aatctcact caactacaa gttttagac 
      ctcagccac aatcaacta gcatatcct caaattttc 
      cagaagttc aatattccg gaaaggcac cctccgtta 
      caaatatca ctcgctttt aataaattg acgcacttg 
      gtaattgaa gaaaacaac tttcggcaa catattgtg 
      tttttgtat gggaatccc tggaactgc aagtgttgg 
      caaatgatg gaaaagttc atgcttgac aataaagtg 
      aaacgaaat gtgtgtgat ttgaagttt ttcggtaat 
      ggtgacgtt ccctattgt attgaattt gacaaaagt 
      gactgccgg agcggcgat ttcggcaaa gattcgaac 
      atttctcaa cgcgatatt caaaatttt gtcaaagtc 
      gtagaaggc agcttggat atgataagg tgtaggttg 
      acaccgaga atattgtag
    mRNA sequence
    • >TC034652 RA
      cgactcaag agaacttaa attcgatga actcaatcg 
      tgcttaact tacaaattg cctgctacg ttctcacat 
      ttctgtcga atttattcg cgaattggt tgaactcgc 
      ggagatatc tggagtgtt taaaacgcc tgggaacgt 
      tggacgagt gtttgtaca agtttggct accaaatca 
      actacaaga agatgaacc gagtgtttt actggtctt 
      tgcttctct cggttggtt tattcttgt atgttgctc 
      tagccgact gtgttttca gcgtgtttc tgaaccaga 
      atatttcgt ttatcaatt ggttgtctt cttacgtgc 
      cgcttacca ctaattact tctcctcgt attttatcg 
      taatgtctt tgtactttc tccacgtta gatattctt 
      atttgagtc agtgtttca agagttatt accctactg 
      gtgacctca caactttac gaaacgttg ttaaaatgt 
      tgactgata ttaaggata tcagtaact cgttgattt 
      cgcagtttg ctctccaaa tctgtgctt tattcattt 
      taacatttc tcacagtga ctaacaatc tgttcctaa 
      cagctaaag gcacatttc attcgttcc aatacttta 
      tcctgatta atatcgcgt gcttgcatg ttgttgaaa 
      gtgtgtcgg ttgttttgg cccattatc agattcact 
      taaagcttg cagagataa cagatttgg ctccgaaag 
      ttatgtctt tacgaactt tgttaccaa atcacagca 
      acgaaatca tggaatttt acttactaa tgtgcaata 
      ctagacttg atgttcaag tgtttggaa tgtttacag 
      taacaccat cagcactgc ttggtatat cggcatgga 
      ttgggtctt atttggtcc tcaccgtcg tctttgcaa 
      gcgtgctgt tttacactg ggaccgaat caatgtgcc 
      ggcactctt atatgacgt tttgtgata atttgagcg 
      accttcaga actacaacg ttgaaaatt ggacggaac 
      ttgtggtag gaacagagt cacatgatg attcgcaac 
      ttcaggcag tcgattccg actcgcttt cattagaca 
      ggttggaaa aagtggtta ctttcatta ttatcgggc 
      aaattagag acatcaaga cgtacacgt tcaccctcg 
      atccgtttt tcaaccgct tgaactatt tacagttgt 
      atggaaatg atataaggc gcataagat ttacgactt 
      ttgacacga tgaagttaa caaagttaa gtttagtga 
      acaacaaga ttgagtata ttcatgcgg gggcgttct 
      ataattgcg aaatcaaaa ctatagatt tgtcgcaca 
      ataagctgg agacgattg aaacagacg tgttttcgg 
      aagagttct tctataatt caaccaaag aaatagtga 
      taagatata acgaaatcg gcgctattc agtccgata 
      gcttcccga gcagcctgg agatcctca atttagacc 
      acaacaatc taaaggtgt taaatgctg atgttttcg 
      acaatttgg ataacttac aagaactaa cactcagcc 
      acaaccaat tatcatcgt taggattcg taaaaaacc 
      tcgaaaaac tcaaactac ttgacgttt cgcacaaca 
      aaattgtcg ctctcacca gtcaacact tcaacaatc 
      tcactcaac tacaagttt tagacctca gccacaatc 
      aactagcat atcctcaaa ttttccaga agttcaata 
      ttccggaaa ggcaccctc cgttacaaa tatcactcg 
      cttttaata aattgacgc acttggtaa ttgaagaaa 
      acaactttc ggcaacata ttgtgtttt tgtatggga 
      atccctgga actgcaagt gttggcaaa tgatggaaa 
      agttcatgc ttgacaata aagtgaaac gaaatgtgt 
      gtgatttga agtttttcg gtaatggtg acgttccct 
      attgtattg aatttgaca aaagtgact gccggagcg 
      gcgatttcg gcaaagatt cgaacattt ctcaacgcg 
      atattcaaa attttgtca aagtcgtag aaggcagct 
      tggatatga taaggtgta ggttgacac cgagaatat 
      tgtagattt tctaagtga taattaagt attactcgg 
      ttataagtt tgttgtatg atgtattta ataaaactc 
      agatcgctt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY