Status
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Gene information
General information
TC032780
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007540,TC030406
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3173926..3181506
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032780
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09870

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032780 RA
      MSAKIIPVP KTSIKRLQV SSFWSKYFP TDPETCNLR 
      MTVTGLVLS FLAVAVIFD KFGDLRVFD SIGVFLVCG 
      YIVLVAWVS YEFYNGLKI ADPRRYTTW IIVKVFEIF 
      LIVASCVYG IYDGSLPVI LVFGVIVLM LDGFLTFVV 
      LKLWQRQLD RQGTYTSIK RSMTHGISM RVYMVRLIE 
      YKILLFTVS VTSLTVCSL VISIGGLEI YVDLKNLSA 
      GDGSASFMT HKLVSFAIL CTANILIFV NMAYMSVHA 
      LRAERYNWY VSCHYFLYI VCELASALV IFTEFDEFY 
      HPIGLASLV AVQFVLLFM ITCFAELIF DRVEDLLAE 
      ENESLLHKR TVATIRRRT KKRMPCVRI DLHNTKGAG 
      QEAHSRIKS EIRITTVLS VLNFIITLY ASYWYVYPI 
      EGDKEIYYA LKFFEEYCP RHKMVLSVV YRATFPLLS 
      YAMIVQAYQ VIYTTQHIR FQAILFIEF VLNIGHQTK 
      NLSEEKLFY DTDYQKIVG ERFKFCIMR HHEFIAFRR 
      LKLNEMSNL IVGFSILGC LLLFSFGLF VLTGKLHRE 
      HFWRFGLGS LAAVCTFGS VIWAGQSIE IESENVVNS 
      LNSVKWYTF DENNKRNYI IMLVNTMQP YKLKFSENF 
      SINYSLGVS TTIHNHITG YRNESDPCV SFSANVKLL 
      RQGRAKSAG ETQVKMLSQ NIVTILVLL MLQVALGVD 
      PQVYETVDE KSNTRIVRD PFNQPRARL YKPQTFNYQ 
      SALQQNQRM FRYAKQQRV DMQNIVKRA QTQAIDPAV 
      ASSTKHENY KLPQEKTNT TSLYSMWHK ISQLSTTRK 
      PVTSRKMKP STYYEDFDG KIKRQDNNQ LVYIKNQRS 
      EHDLNALSA LVGQPPSNQ LEGLKRLLE STQSSLVLP 
      PIKGPLPTP VIEHSVDIP NPRTHPNVD QNLSLEALQ 
      SQLDENAKA QLAKALADA QQQAFAHVE AQHQAIAKA 
      QVEAQKKAL AQIALHNQG IKGERLPQP LPLKQHISQ 
      PLTARNETV IKPKTSAKP RQSPKAYQQ VVLETQSQS 
      QKVLPEINH QLKLDGVDD HTYAARYAF GYKIRDVKM 
      GNEFGHEEK REGENAKGH YHVLLPDGR MQKVEYFAG 
      PSGYHAKVT YENLAHH
    CDS sequence
    • >TC032780 RA
      atgtcggca aaaataatt ccagtgccc aaaacctct 
      ataaagcgc cttcaagtc tcgtctttc tggagcaag 
      tacttcccc actgaccca gaaacgtgt aacttaaga 
      atgacggtt actggtttg gttctgtca tttttagca 
      gtggctgtt attttcgac aaattcggt gatttgcgt 
      gtttttgac tcaattgga gttttccta gtttgtgga 
      tacattgta ctagttgcg tgggttagc tatgagttc 
      tacaatggt ttaaaaatt gccgatccg aggcgatat 
      accacatgg attatcgtc aaggtgttt gaaattttt 
      ttgatagta gcttcttgt gtatatggg atttatgat 
      ggttctttg ccggtgata cttgtcttc ggtgtcatc 
      gttttaatg cttgatggt tttcttact tttgtcgtc 
      ctcaaatta tggcaaagg caattggat cgacaaggg 
      acctacact agtattaag aggagcatg acccacggt 
      atatccatg agggtctac atggtacgt ctgattgag 
      tacaaaatc ctcctattc acggtttcc gttacgtct 
      ttgacagtt tgttcttta gtaatttca attggaggt 
      cttgagatc tacgtggac ctaaagaat cttagtgca 
      ggtgacggt tctgcctcc ttcatgacg cataaacta 
      gtctcgttt gcgattctc tgcactgca aatatttta 
      atctttgta aatatggca tacatgtca gttcatgct 
      ttacgtgcg gaaaggtac aactggtac gtcagttgc 
      cactacttt ttatacatt gtgtgtgaa ttagcctct 
      gctttagtc atttttacc gaatttgat gaattttac 
      catccgata ggattagcg agcttggtc gctgtacag 
      tttgtattg ttatttatg atcacctgt tttgctgag 
      ttgattttt gacagagtt gaggatctg cttgccgaa 
      gaaaatgag agtctactt cataaaaga accgtagct 
      actatcaga agacgcacc aaaaagcgt atgccgtgt 
      gttaggatc gacttgcat aacaccaaa ggtgccgga 
      caggaagct cactcgcgc attaaatcc gaaataagg 
      attacaact gtactgtct gtgttaaat tttataatc 
      actctgtat gcgtcttac tggtacgtt tacccaatc 
      gagggtgat aaggaaatc tactatgcg ttaaaattt 
      ttcgaggag tattgccca cgtcacaaa atggttctc 
      agtgtcgtt tacagggca acatttccg ttgttgtcg 
      tacgctatg attgtgcag gcgtatcaa gtcatttac 
      accacccaa cacatacga tttcaggca attttattt 
      attgaattt gttttaaat atcggacac caaacgaaa 
      aatctctcc gaggagaag cttttttat gatacagat 
      tatcaaaaa attgttggg gaaagattc aagttttgc 
      ataatgagg caccacgaa tttatagcg tttcgtcga 
      ttgaaatta aatgaaatg agcaatcta atagttggg 
      ttttcgata cttggatgt ttgctatta ttttctttt 
      gggcttttt gttttaact ggcaaactt caccgcgag 
      cacttttgg cgttttggt ttgggatca ttggctgct 
      gtgtgcaca tttgggtct gtaatatgg gccggacag 
      tctatcgaa attgagtcc gagaatgtt gtaaattca 
      ttgaattca gtcaaatgg tatacattt gatgaaaat 
      aataagagg aactacata atcatgttg gttaatacg 
      atgcagcct tacaaactt aaattttcg gaaaatttt 
      tcgattaat tactcctta ggagtttcc acgaccatc 
      cataatcac attacgggg tatagaaat gaaagtgat 
      ccgtgtgtt tccttcagt gccaatgtt aagctcttg 
      aggcaaggt cgagcaaaa agtgccggt gaaacccaa 
      gtcaaaatg ctaagtcaa aatattgtc acaatttta 
      gttctgctt atgttgcaa gtggcgtta ggagttgac 
      cctcaagta tatgaaact gtggacgaa aaatccaat 
      actagaata gtacgagat ccctttaat caaccaagg 
      gctaggctg tacaaaccc caaaccttc aattatcaa 
      agtgcactt caacaaaac caacgaatg tttcgctac 
      gcgaaacaa caacgcgtc gatatgcaa aatattgta 
      aaaagggcc caaacacaa gccatcgac cccgcagtc 
      gcttcgtcg acaaaacac gaaaattac aagcttccg 
      caagaaaaa accaacacg acatcgctc tattcgatg 
      tggcataag atctcgcaa ttatcaacc acgagaaaa 
      ccggtaacg agtcgcaaa atgaaacct agcacttac 
      tacgaagac tttgatggc aaaatcaaa cggcaagac 
      aacaaccaa cttgtctac atcaaaaac caacgctct 
      gaacacgat ttaaacgca ctgagtgcc ttggtggga 
      caaccaccc agcaatcag ttggaaggt ttgaaacgt 
      ctccttgaa tcaactcag tctagtcta gtcctgcct 
      ccaatcaaa ggcccttta ccaacaccc gtcatcgaa 
      cacagtgtt gatatacca aacccccga actcaccca 
      aacgtcgac cagaacctc tcccttgaa gcccttcaa 
      tcccaactg gacgaaaac gcgaaagct caactcgcc 
      aaagctcta gctgatgcc caacaacaa gcttttgcc 
      cacgtcgaa gcccagcac caggccata gccaaagcc 
      caggtcgaa gcccagaaa aaggcctta gcccaaatt 
      gcgctccac aaccaaggg atcaaagga gaacgactt 
      ccacaaccc ctacccctc aaacaacac atatcacaa 
      cccctaact gcccgaaac gaaactgta attaaaccg 
      aagacaagt gcgaaacct cgccagagt cctaaagct 
      tatcaacaa gtggtttta gagactcag agtcaaagc 
      caaaaagtc cttcccgag atcaaccat cagttgaaa 
      cttgacggt gttgacgat catacatat gccgcacga 
      tacgctttc ggctacaag atcagagac gtcaaaatg 
      gggaacgag ttcggacat gaggaaaaa agggaagga 
      gagaacgcc aaaggacac taccatgtc ctgctgccg 
      gacggcagg atgcagaaa gtggagtat tttgccgga 
      ccaagcgga tatcacgcg aaagtcact tacgaaaat 
      ctcgcacat cattaa
    mRNA sequence
    • >TC032780 RA
      agactcgta aaaatgggt gaatcactt cgtacatac 
      gtaaattgt aaacaatca tgttcgttc gttaagtac 
      gtgtgaatg gccagaatt aatgtaaaa tgtcggcaa 
      aaataattc cagtgccca aaacctcta taaagcgcc 
      ttcaagtct cgtctttct ggagcaagt acttcccca 
      ctgacccag aaacgtgta acttaagaa tgacggtta 
      ctggtttgg ttctgtcat ttttagcag tggctgtta 
      ttttcgaca aattcggtg atttgcgtg tttttgact 
      caattggag ttttcctag tttgtggat acattgtac 
      tagttgcgt gggttagct atgagttct acaatggtt 
      taaaaattg ccgatccga ggcgatata ccacatgga 
      ttatcgtca aggtgtttg aaatttttt tgatagtag 
      cttcttgtg tatatggga tttatgatg gttctttgc 
      cggtgatac ttgtcttcg gtgtcatcg ttttaatgc 
      ttgatggtt ttcttactt ttgtcgtcc tcaaattat 
      ggcaaaggc aattggatc gacaaggga cctacacta 
      gtattaaga ggagcatga cccacggta tatccatga 
      gggtctaca tggtacgtc tgattgagt acaaaatcc 
      tcctattca cggtttccg ttacgtctt tgacagttt 
      gttctttag taatttcaa ttggaggtc ttgagatct 
      acgtggacc taaagaatc ttagtgcag gtgacggtt 
      ctgcctcct tcatgacgc ataaactag tctcgtttg 
      cgattctct gcactgcaa atattttaa tctttgtaa 
      atatggcat acatgtcag ttcatgctt tacgtgcgg 
      aaaggtaca actggtacg tcagttgcc actactttt 
      tatacattg tgtgtgaat tagcctctg ctttagtca 
      tttttaccg aatttgatg aattttacc atccgatag 
      gattagcga gcttggtcg ctgtacagt ttgtattgt 
      tatttatga tcacctgtt ttgctgagt tgatttttg 
      acagagttg aggatctgc ttgccgaag aaaatgaga 
      gtctacttc ataaaagaa ccgtagcta ctatcagaa 
      gacgcacca aaaagcgta tgccgtgtg ttaggatcg 
      acttgcata acaccaaag gtgccggac aggaagctc 
      actcgcgca ttaaatccg aaataagga ttacaactg 
      tactgtctg tgttaaatt ttataatca ctctgtatg 
      cgtcttact ggtacgttt acccaatcg agggtgata 
      aggaaatct actatgcgt taaaatttt tcgaggagt 
      attgcccac gtcacaaaa tggttctca gtgtcgttt 
      acagggcaa catttccgt tgttgtcgt acgctatga 
      ttgtgcagg cgtatcaag tcatttaca ccacccaac 
      acatacgat ttcaggcaa ttttattta ttgaatttg 
      ttttaaata tcggacacc aaacgaaaa atctctccg 
      aggagaagc ttttttatg atacagatt atcaaaaaa 
      ttgttgggg aaagattca agttttgca taatgaggc 
      accacgaat ttatagcgt ttcgtcgat tgaaattaa 
      atgaaatga gcaatctaa tagttgggt tttcgatac 
      ttggatgtt tgctattat tttcttttg ggctttttg 
      ttttaactg gcaaacttc accgcgagc acttttggc 
      gttttggtt tgggatcat tggctgctg tgtgcacat 
      ttgggtctg taatatggg ccggacagt ctatcgaaa 
      ttgagtccg agaatgttg taaattcat tgaattcag 
      tcaaatggt atacatttg atgaaaata ataagagga 
      actacataa tcatgttgg ttaatacga tgcagcctt 
      acaaactta aattttcgg aaaattttt cgattaatt 
      actccttag gagtttcca cgaccatcc ataatcaca 
      ttacggggt atagaaatg aaagtgatc cgtgtgttt 
      ccttcagtg ccaatgtta agctcttga ggcaaggtc 
      gagcaaaaa gtgccggtg aaacccaag tcaaaatgc 
      taagtcaaa atattgtca caattttag ttctgctta 
      tgttgcaag tggcgttag gagttgacc ctcaagtat 
      atgaaactg tggacgaaa aatccaata ctagaatag 
      tacgagatc cctttaatc aaccaaggg ctaggctgt 
      acaaacccc aaaccttca attatcaaa gtgcacttc 
      aacaaaacc aacgaatgt ttcgctacg cgaaacaac 
      aacgcgtcg atatgcaaa atattgtaa aaagggccc 
      aaacacaag ccatcgacc ccgcagtcg cttcgtcga 
      caaaacacg aaaattaca agcttccgc aagaaaaaa 
      ccaacacga catcgctct attcgatgt ggcataaga 
      tctcgcaat tatcaacca cgagaaaac cggtaacga 
      gtcgcaaaa tgaaaccta gcacttact acgaagact 
      ttgatggca aaatcaaac ggcaagaca acaaccaac 
      ttgtctaca tcaaaaacc aacgctctg aacacgatt 
      taaacgcac tgagtgcct tggtgggac aaccaccca 
      gcaatcagt tggaaggtt tgaaacgtc tccttgaat 
      caactcagt ctagtctag tcctgcctc caatcaaag 
      gccctttac caacacccg tcatcgaac acagtgttg 
      atataccaa acccccgaa ctcacccaa acgtcgacc 
      agaacctct cccttgaag cccttcaat cccaactgg 
      acgaaaacg cgaaagctc aactcgcca aagctctag 
      ctgatgccc aacaacaag cttttgccc acgtcgaag 
      cccagcacc aggccatag ccaaagccc aggtcgaag 
      cccagaaaa aggccttag cccaaattg cgctccaca 
      accaaggga tcaaaggag aacgacttc cacaacccc 
      tacccctca aacaacaca tatcacaac ccctaactg 
      cccgaaacg aaactgtaa ttaaaccga agacaagtg 
      cgaaacctc gccagagtc ctaaagctt atcaacaag 
      tggttttag agactcaga gtcaaagcc aaaaagtcc 
      ttcccgaga tcaaccatc agttgaaac ttgacggtg 
      ttgacgatc atacatatg ccgcacgat acgctttcg 
      gctacaaga tcagagacg tcaaaatgg ggaacgagt 
      tcggacatg aggaaaaaa gggaaggag agaacgcca 
      aaggacact accatgtcc tgctgccgg acggcagga 
      tgcagaaag tggagtatt ttgccggac caagcggat 
      atcacgcga aagtcactt acgaaaatc tcgcacatc 
      attaactga gttcgttta cttagtctg aaaggtaaa 
      aagatgata aaaaactgt gtaaaaggt tcttgagtt 
      atcacaaga tacgtgact tttctcctg tttaaaaca 
      ctgtcagtg tctcatttt attttctgt aatttgctg 
      agaactaaa tctactcat attttattg gaatatcaa 
      gtttggaaa aaaaacgtc tgccatttg atgatttaa 
      tagaaagta gtgcctaga ctataaatc ctcggctac 
      aaaatactc acctaattg atcatctgc agcatctgg 
      gtgtgtttt taattgctc ctatttatc tcatagaca 
      cacaaatgc aacaggtgg tacaaatgg ctttaagcc 
      gaatttttt agggatttg tgcaataat gtttagact 
      gagctcgtc gaacattgt cttagttct agtatttat 
      gtttgattt atgttgttg tttgtaatt aaacctctt 
      taaacaaat taagtgcag tgcatttat ttaccactc 
      ctatcaatt ttttgacga ataaaattt ctatgaacc 
      t
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