| General information | |||
| TC007557 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3031963..3039280 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC007557 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09869 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007557 RA
MENEIFCKR KGSTDKGKL YEDLVTADL ILKLLTEQN VNNFKLSSN NEEFGAFDD VVLKLDTGE IYALQLKHS DNNDKAVCE IDLTGGKNF KPRFDLKKY YESYKNIKV PCKVILFTN RTCDISDQE FPLNQNTTI KCLKCSTNP ISLLNHSEN GTWHTFEVV RTSPKQRDD YQAFFNNFY LFCGQANVE ELRAKIIAR FQETISCSK SLCDQFLQF VSSWSFREG EKEMLDKTT MQRVLVLFL LERSIQGFD FKPVNQERK LLQEVISLF HVTLIGEDS CEKVKQIWG DFGDEFENN GAEVAKIRA KYQIKSFDE LDSSDKAKI LWLMGKCPL VIAADNGTM SKVLQLCPN EKFVLLTSH ENVITLEGS FFRNLSDLD LSSDICQKI LRTFCCSIQ GKDKRSLKC FVDRNKNFR ENVTVSDLI NMTDGPRLI GEEKEKAPD PYITRFLTT NLINIKYLT TVDENTLVV LDCMNKIDK IKRHLGNFN LHKVATYLK DLSLYNQDS NQVTLFHSS PIIKQFVSG PDMYVSEAQ CTPVEFEEI CQRNKNKEK CHHFRVIDE MTLEWIRSR KSITTLEQF RIDGTYIKE SSLYGLEPE NNVNLVNGD PGMGKSELL KNIKNNSNP EILCIFFPS KDTTLLCSQ LEGKSDLYE AFVSFILNT KYRAFPSFD KKVIKFLID TDQVMYFWD ALDEVSTKN MKTTIDLIE ILKGKSLCQ WVSSRCHLK GHLEKKFNV LSRNINQFD EEEQSLYIH KRLGRLYSE DDINKVIEK IRSAVTLMK HNDILGIPL QVFMLTELF LQDKEKYFN LLKNMFSLA DLYHYIIEE KFNVYYKEK AGISSPNDA LERILMFHR KNMYENYEK AALKASITK EILKRFHIN CDNFLKELE TGDDNIGLI KEVTNSFPQ FQHNSYAEY FVATYFIAH YEEIPELRS LLFDKRHAN IRFFFDLLT AKDSPSLVA VLCKNIDLL KKHQEDLER SEDKRGRNA LQLACSWGQ RYPILEVEK DNGTWILDN TNDNTLCGE TPEYNEILQ YLLEHCNVT ETCKLFNLS CVRYAEESD CLMTKLKIL LKQNNKFSK LKNDKEGIS ILYYCTKFG YDEVIDLFE DLPFIRMKV NDFTLLHLA VECEQEKYL RRLLQVPGY QKTIDKKDK FGRTPLYLA CQLGLYKIV ELLIESGAN VNYSTPLHI ACLHAHDNI VKLLLKAGT DINATDSYN ITPLHTASW NGHHTTVQI LTANKNVEI DVQTSGGCT PLYVAARNK HDRVVELLI EAKANVNLA QKENVTPLM IASQEGHQE NVKLLIQAG SDVNQVATT GESPLYVAC QNGHDVVVE MLLEAGANT EAFADNGWS PVFVACQQG HVGVVQLLM KAHANLGIK DKTGRTPLM EKIVIKIKL KVPDRGKNV RLEQKILKT CVSLLIMDL DDLLGPHYL NLNFSDDFL NESAPKLSI ELPYPIHKP LKDLNLDII DEFHQNASL YSHKLSTLH KDFRFARDK VMNQTFTDS HATITRGLL DFCNTEKPG IQFPCDYNP YYTSGFLEY VILDNVEYL MRPDCDNNI RLTKLAKPW KNRKIKVEV PFEDNITGI SFSSGSSCL LVRHKSHIN LLKLTDLEG EIRQTKKFK KFLLDSQIC EGQFGVVLS DTKFRLYDV ETNKRISLD DKTVDSVTR CEFANEMII LLGRKKLKL FDKRSNDFE IFEPELQPC NFFCSVKAH GDSLYVGSR HCLMRTDVR FLKNFDFCT HLMKYGPCY MDLVEGFLC LSSQKVGEK VMFSGEPIS SLPFKVPSI VETFSECRL KKDVLLVDG IKERVAKSV GGVKIIHEN NDYFLYSAN SLGDIFKQK ITTNSDCDE TKPVDSFHY WVNKLPEKP KPLHLTSVE NASLARFVL NKGIDHEKS KKYSPRI
- >TC007557 RA
atggaaaac gaaattttt tgcaaacgc aagggatca acggacaaa ggaaaactt tacgaagat ttagtcacc gctgattta attctaaaa ttattgact gaacaaaat gtaaataat tttaaactc tcatccaat aatgaagaa ttcggagct ttcgacgat gtagtttta aaacttgat actggggag atctacgcc ttgcaactg aaacactca gataacaac gataaagct gtatgtgaa atagattta acaggaggg aagaatttc aaacctcgg ttcgacctc aaaaaatac tacgagtct tataaaaac atcaaagtg ccttgtaaa gtcatactc ttcactaat cgaacctgc gatattagt gaccaagaa tttcctttg aaccaaaac accacaata aaatgttta aagtgcagc accaatcca ataagctta ctgaaccat tctgaaaac ggcacttgg cacactttt gaggtcgtt agaacttca cctaaacaa cgtgacgat tatcaggcc ttcttcaac aatttttac ctcttttgt ggacaagca aacgtcgaa gaacttaga gctaaaatc atagcacga tttcaagaa accatctct tgtagcaaa tcgctttgc gaccaattt ctccaattc gtatcaagc tggagtttc cgcgaagga gagaaagaa atgttggac aaaaccaca atgcaacgt gtgttagtt cttttccta ctggaacgc tcaatccaa ggatttgac tttaaaccc gtcaaccaa gagagaaaa ctgttacaa gaagtgatc tctctgttt catgttaca ctcatcgga gaggattct tgcgaaaaa gttaaacaa atttgggga gattttggc gacgaattt gaaaataat ggagcagaa gtggcaaaa atacgagcc aaatatcaa ataaaatca ttcgatgaa ttagatagt agtgacaaa gcaaaaatt ttgtggttg atgggaaag tgtcctcta gttattgca gctgataat gggacaatg agtaaggta ttgcaactg tgtcccaac gaaaaattt gttttgctc acaagccat gagaatgtg atcacactg gagggttct ttttttcga aatttatca gacttggat ttaagttca gatatttgc caaaaaatt ctccgcacg ttttgctgc tccattcaa ggaaaagac aagaggagt ttaaaatgt ttcgttgat cggaataaa aattttcga gaaaacgta actgtcagt gatttaata aatatgacg gatggtcct cgcctcatt ggagaagaa aaagaaaaa gctccagac ccgtacatt accagattt ttgacaact aatttaata aacataaaa tatttaaca actgtcgat gaaaacaca ctcgtcgtt cttgactgc atgaataaa attgataag atcaaaagg cacttggga aattttaat ctgcacaaa gttgcaaca tatctgaag gatctttcg ctttataac caggattca aaccaagta acactgttc cattcaagc ccaatcata aaacaattt gtttcgggt cccgatatg tacgtaagc gaggcccaa tgtacaccg gtggaattt gaagagatt tgtcaaaga aacaaaaac aaggagaag tgccaccac ttcagagta atcgacgaa atgacatta gaatggata aggagcaga aaaagcatc acaacttta gagcaattc cgaattgat ggaacctac attaaagaa tcgagtctt tacggctta gagccggaa aacaacgta aatttagtg aacggagac cctggaatg ggaaaatcg gagttgctg aaaaatatc aagaacaat tcaaatccg gagattctg tgcatcttt ttcccttcg aaagataca actctactt tgctcacag cttgaaggc aaaagtgac ttgtacgag gcttttgtc agtttcatt cttaatacg aaatatcgg gcgttcccg agtttcgat aaaaaagtc ataaagttt ttaattgac acagatcaa gtgatgtac ttttgggac gccttggac gaagtttcc acaaaaaat atgaagaca actatagac cttattgag atattgaaa ggtaaaagt ttgtgtcag tgggtgtcc agtcgctgc cacctcaag ggtcatctg gagaagaaa ttcaacgtt ctttccaga aacataaac cagtttgac gaagaggaa caatctctc tacatacat aaacggctt ggacggtta tattcagag gacgacatc aacaaagtg attgaaaaa atccgatcc gctgtcact ttaatgaag cacaacgac attttgggc atccctttg caagtgttt atgctaacc gagctcttc cttcaagac aaggaaaag tacttcaat cttttgaaa aacatgttt tctttagcc gacctctac cactacatc atcgaggag aaattcaac gtctattac aaggagaaa gccgggatt tcttcacct aacgacgct ttagagcga atcctgatg ttccaccgc aaaaacatg tacgaaaat tacgaaaaa gccgccttg aaggcctca atcactaag gaaattctc aaacgtttc cacataaac tgcgacaat tttttaaaa gagcttgaa accggcgac gacaacatt ggcttgatc aaagaggtc actaacagt tttccccag tttcagcac aattcctat gctgagtat ttcgtcgcc acttacttt atcgcacat tatgaggaa atacccgag ttgcggagc cttctgttc gacaagcga cacgcaaat atccgattc ttcttcgac ctgctgacg gcgaaagac tcgccttcg cttgtggca gttttgtgc aaaaacatc gacttgttg aaaaaacac caagaggat ttggagcgc agcgaggac aaacgaggc agaaacgcc ctacaactg gcgtgctcg tggggacag aggtacccg attttagaa gttgaaaag gataacggg acttggatt ttggacaac accaatgat aacactctt tgtggcgaa acgccggag tataacgaa attttgcag tatctttta gagcactgt aacgtgacg gaaacttgc aagttgttt aatttgtcg tgtgtgagg tatgccgaa gagagcgat tgtcttatg acgaaactg aagattctc ttgaagcag aataacaag ttttcgaaa ttgaagaac gacaaggaa gggatcagc attctgtac tattgcacc aagtttggc tacgacgaa gttatcgac ttgtttgaa gatttgccc tttattagg atgaaagtc aacgatttt acgttatta cacctagct gttgagtgc gaacaagaa aaatatctg agacgactc cttcaagtt ccaggatat caaaaaacc atagataaa aaagataaa ttcgggaga acaccactt tatttagct tgtcagtta ggtttatac aaaattgtt gaattattg atcgaaagt ggagccaat gttaattac tcaacacct cttcatatc gcttgcctc cacgcccat gacaacatc gtcaaattg ctcctgaaa gccggaaca gacataaac gccaccgac agttacaac ataactcct ctgcacaca gcttcctgg aacggccac cacaccact gtccaaatc ctaactgca aacaaaaat gttgaaatc gatgttcaa acatcaggt ggttgcact cctctctac gtggctgca agaaacaag catgacaga gttgtggaa ttgttgatt gaagcaaag gccaatgtt aatctagcg caaaaagaa aatgttaca cctcttatg atagcatca caagaagga caccaagaa aatgttaag ctgctgatt caagctggt tctgatgtc aatcaagtg gcaacgacg ggggaatca ccactttac gtggcttgc cagaatggt cacgatgtt gtggttgaa atgcttcta gaagcggga gcaaatacc gaagcgttt gcagacaat ggatggagt cctgttttt gtagcgtgt cagcaagga catgttggg gttgttcag ttgttaatg aaagctcat gcaaatttg ggtattaaa gataagact ggaagaacc cctttgatg gaaaaaatt gtaattaaa attaaatta aaggttcct gaccgcggc aaaaatgta aggttagaa caaaaaatt ttgaagact tgtgtgagc cttttaatt atggattta gacgacctg ttaggcccc cattacttg aacttaaat ttcagtgac gatttttta aatgaaagt gcaccaaaa ctctcaatt gaactgcct tatcctatt cacaagccg ctgaaagac ctaaacctt gacataatt gatgagttt caccaaaat gcgagcctt tactctcac aaattatca accttacat aaagatttt cgttttgcg agagataaa gtcatgaat cagacgttt acagactca catgctaca attacaagg gggttgtta gacttttgt aatacggaa aagcccgga attcaattc ccctgcgat tacaaccct tactacacc tcggggttt cttgaatat gttattttg gacaatgtg gagtattta atgcgacca gattgcgac aataacatt cggttgaca aaattggca aagccttgg aaaaataga aaaatcaaa gttgaagtt ccatttgaa gataacata actggaatc tcatttagt agtggttcc agttgttta ttagtgaga cacaaaagt cacataaac ttgctcaaa ctgaccgat cttgaggga gaaataaga caaacaaaa aagttcaaa aagtttctc cttgattcc caaatctgc gaaggacaa tttggagtc gttctctcc gacacaaaa ttcagatta tacgacgtt gaaactaac aaaagaatt agtctcgac gataaaact gttgactcc gtcactcgt tgtgaattt gcgaatgag atgattatt cttttgggc cggaaaaaa ctcaaactt tttgataaa cgttcaaat gactttgaa atttttgag ccggaattg cagccttgt aatttcttc tgctctgtt aaagcccac ggggactca ctttatgtg ggctcaaga cattgttta atgcgaact gatgtcagg tttttgaag aatttcgat ttttgcacg catttgatg aaatacggg ccttgttac atggactta gttgaaggt ttcttgtgt ctttcgagt cagaaagtt ggggagaaa gttatgttc agtggagag cctattagt agtttgcct tttaaagtc ccgagtatc gttgaaact ttcagtgag tgtcggtta aaaaaggat gtattatta gtggatggt attaaggaa agagttgca aaatcagtt ggaggtgtt aaaataatt cacgaaaat aatgattat ttcctctac agtgctaac agtttgggc gatattttt aaacaaaaa atcacaacc aactcagac tgtgatgaa acaaaacca gtggactcg tttcactat tgggtgaat aaactacca gaaaagccc aagccgttg cacttgacc agtgttgaa aatgcaagc ttggcaagg tttgttcta aacaaaggt atcgaccat gagaaatcg aaaaaatac agccctcga atttga
- >TC007557 RA
tcaacacac tgaagtgcc aacaaaaat ttttatatt aatttaaaa tggaaaacg aaatttttt gcaaacgca agggatcaa cggacaaag gaaaacttt acgaagatt tagtcaccg ctgatttaa ttctaaaat tattgactg aacaaaatg taaataatt ttaaactct catccaata atgaagaat tcggagctt tcgacgatg tagttttaa aacttgata ctggggaga tctacgcct tgcaactga aacactcag ataacaacg ataaagctg tatgtgaaa tagatttaa caggaggga agaatttca aacctcggt tcgacctca aaaaatact acgagtctt ataaaaaca tcaaagtgc cttgtaaag tcatactct tcactaatc gaacctgcg atattagtg accaagaat ttcctttga accaaaaca ccacaataa aatgtttaa agtgcagca ccaatccaa taagcttac tgaaccatt ctgaaaacg gcacttggc acacttttg aggtcgtta gaacttcac ctaaacaac gtgacgatt atcaggcct tcttcaaca atttttacc tcttttgtg gacaagcaa acgtcgaag aacttagag ctaaaatca tagcacgat ttcaagaaa ccatctctt gtagcaaat cgctttgcg accaatttc tccaattcg tatcaagct ggagtttcc gcgaaggag agaaagaaa tgttggaca aaaccacaa tgcaacgtg tgttagttc ttttcctac tggaacgct caatccaag gatttgact ttaaacccg tcaaccaag agagaaaac tgttacaag aagtgatct ctctgtttc atgttacac tcatcggag aggattctt gcgaaaaag ttaaacaaa tttggggag attttggcg acgaatttg aaaataatg gagcagaag tggcaaaaa tacgagcca aatatcaaa taaaatcat tcgatgaat tagatagta gtgacaaag caaaaattt tgtggttga tgggaaagt gtcctctag ttattgcag ctgataatg ggacaatga gtaaggtat tgcaactgt gtcccaacg aaaaatttg ttttgctca caagccatg agaatgtga tcacactgg agggttctt tttttcgaa atttatcag acttggatt taagttcag atatttgcc aaaaaattc tccgcacgt tttgctgct ccattcaag gaaaagaca agaggagtt taaaatgtt tcgttgatc ggaataaaa attttcgag aaaacgtaa ctgtcagtg atttaataa atatgacgg atggtcctc gcctcattg gagaagaaa aagaaaaag ctccagacc cgtacatta ccagatttt tgacaacta atttaataa acataaaat atttaacaa ctgtcgatg aaaacacac tcgtcgttc ttgactgca tgaataaaa ttgataaga tcaaaaggc acttgggaa attttaatc tgcacaaag ttgcaacat atctgaagg atctttcgc tttataacc aggattcaa accaagtaa cactgttcc attcaagcc caatcataa aacaatttg tttcgggtc ccgatatgt acgtaagcg aggcccaat gtacaccgg tggaatttg aagagattt gtcaaagaa acaaaaaca aggagaagt gccaccact tcagagtaa tcgacgaaa tgacattag aatggataa ggagcagaa aaagcatca caactttag agcaattcc gaattgatg gaacctaca ttaaagaat cgagtcttt acggcttag agccggaaa acaacgtaa atttagtga acggagacc ctggaatgg gaaaatcgg agttgctga aaaatatca agaacaatt caaatccgg agattctgt gcatctttt tcccttcga aagatacaa ctctacttt gctcacagc ttgaaggca aaagtgact tgtacgagg cttttgtca gtttcattc ttaatacga aatatcggg cgttcccga gtttcgata aaaaagtca taaagtttt taattgaca cagatcaag tgatgtact tttgggacg ccttggacg aagtttcca caaaaaata tgaagacaa ctatagacc ttattgaga tattgaaag gtaaaagtt tgtgtcagt gggtgtcca gtcgctgcc acctcaagg gtcatctgg agaagaaat tcaacgttc tttccagaa acataaacc agtttgacg aagaggaac aatctctct acatacata aacggcttg gacggttat attcagagg acgacatca acaaagtga ttgaaaaaa tccgatccg ctgtcactt taatgaagc acaacgaca ttttgggca tccctttgc aagtgttta tgctaaccg agctcttcc ttcaagaca aggaaaagt acttcaatc ttttgaaaa acatgtttt ctttagccg acctctacc actacatca tcgaggaga aattcaacg tctattaca aggagaaag ccgggattt cttcaccta acgacgctt tagagcgaa tcctgatgt tccaccgca aaaacatgt acgaaaatt acgaaaaag ccgccttga aggcctcaa tcactaagg aaattctca aacgtttcc acataaact gcgacaatt ttttaaaag agcttgaaa ccggcgacg acaacattg gcttgatca aagaggtca ctaacagtt ttccccagt ttcagcaca attcctatg ctgagtatt tcgtcgcca cttacttta tcgcacatt atgaggaaa tacccgagt tgcggagcc ttctgttcg acaagcgac acgcaaata tccgattct tcttcgacc tgctgacgg cgaaagact cgccttcgc ttgtggcag ttttgtgca aaaacatcg acttgttga aaaaacacc aagaggatt tggagcgca gcgaggaca aacgaggca gaaacgccc tacaactgg cgtgctcgt ggggacaga ggtacccga ttttagaag ttgaaaagg ataacggga cttggattt tggacaaca ccaatgata acactcttt gtggcgaaa cgccggagt ataacgaaa ttttgcagt atcttttag agcactgta acgtgacgg aaacttgca agttgttta atttgtcgt gtgtgaggt atgccgaag agagcgatt gtcttatga cgaaactga agattctct tgaagcaga ataacaagt tttcgaaat tgaagaacg acaaggaag ggatcagca ttctgtact attgcacca agtttggct acgacgaag ttatcgact tgtttgaag atttgccct ttattagga tgaaagtca acgatttta cgttattac acctagctg ttgagtgcg aacaagaaa aatatctga gacgactcc ttcaagttc caggatatc aaaaaacca tagataaaa aagataaat tcgggagaa caccacttt atttagctt gtcagttag gtttataca aaattgttg aattattga tcgaaagtg gagccaatg ttaattact caacacctc ttcatatcg cttgcctcc acgcccatg acaacatcg tcaaattgc tcctgaaag ccggaacag acataaacg ccaccgaca gttacaaca taactcctc tgcacacag cttcctgga acggccacc acaccactg tccaaatcc taactgcaa acaaaaatg ttgaaatcg atgttcaaa catcaggtg gttgcactc ctctctacg tggctgcaa gaaacaagc atgacagag ttgtggaat tgttgattg aagcaaagg ccaatgtta atctagcgc aaaaagaaa atgttacac ctcttatga tagcatcac aagaaggac accaagaaa atgttaagc tgctgattc aagctggtt ctgatgtca atcaagtgg caacgacgg gggaatcac cactttacg tggcttgcc agaatggtc acgatgttg tggttgaaa tgcttctag aagcgggag caaataccg aagcgtttg cagacaatg gatggagtc ctgtttttg tagcgtgtc agcaaggac atgttgggg ttgttcagt tgttaatga aagctcatg caaatttgg gtattaaag ataagactg gaagaaccc ctttgatgg aaaaaattg taattaaaa ttaaattaa aggttcctg accgcggca aaaatgtaa ggttagaac aaaaaattt tgaagactt gtgtgagcc ttttaatta tggatttag acgacctgt taggccccc attacttga acttaaatt tcagtgacg attttttaa atgaaagtg caccaaaac tctcaattg aactgcctt atcctattc acaagccgc tgaaagacc taaaccttg acataattg atgagtttc accaaaatg cgagccttt actctcaca aattatcaa ccttacata aagattttc gttttgcga gagataaag tcatgaatc agacgttta cagactcac atgctacaa ttacaaggg ggttgttag acttttgta atacggaaa agcccggaa ttcaattcc cctgcgatt acaaccctt actacacct cggggtttc ttgaatatg ttattttgg acaatgtgg agtatttaa tgcgaccag attgcgaca ataacattc ggttgacaa aattggcaa agccttgga aaaatagaa aaatcaaag ttgaagttc catttgaag ataacataa ctggaatct catttagta gtggttcca gttgtttat tagtgagac acaaaagtc acataaact tgctcaaac tgaccgatc ttgagggag aaataagac aaacaaaaa agttcaaaa agtttctcc ttgattccc aaatctgcg aaggacaat ttggagtcg ttctctccg acacaaaat tcagattat acgacgttg aaactaaca aaagaatta gtctcgacg ataaaactg ttgactccg tcactcgtt gtgaatttg cgaatgaga tgattattc ttttgggcc ggaaaaaac tcaaacttt ttgataaac gttcaaatg actttgaaa tttttgagc cggaattgc agccttgta atttcttct gctctgtta aagcccacg gggactcac tttatgtgg gctcaagac attgtttaa tgcgaactg atgtcaggt ttttgaaga atttcgatt tttgcacgc atttgatga aatacgggc cttgttaca tggacttag ttgaaggtt tcttgtgtc tttcgagtc agaaagttg gggagaaag ttatgttca gtggagagc ctattagta gtttgcctt ttaaagtcc cgagtatcg ttgaaactt tcagtgagt gtcggttaa aaaaggatg tattattag tggatggta ttaaggaaa gagttgcaa aatcagttg gaggtgtta aaataattc acgaaaata atgattatt tcctctaca gtgctaaca gtttgggcg atattttta aacaaaaaa tcacaacca actcagact gtgatgaaa caaaaccag tggactcgt ttcactatt gggtgaata aactaccag aaaagccca agccgttgc acttgacca gtgttgaaa atgcaagct tggcaaggt ttgttctaa acaaaggta tcgaccatg agaaatcga aaaaataca gccctcgaa tttgagcga gttagctca aaatttctt atcggtttg ggacgacga agacgagga ggagcgcct tgaagtgg
