Status
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Gene information
General information
TC007557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3031963..3039280
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007557
    iBeetle screen iB_09869

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands potentially colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007557 RA
      MENEIFCKR KGSTDKGKL YEDLVTADL ILKLLTEQN 
      VNNFKLSSN NEEFGAFDD VVLKLDTGE IYALQLKHS 
      DNNDKAVCE IDLTGGKNF KPRFDLKKY YESYKNIKV 
      PCKVILFTN RTCDISDQE FPLNQNTTI KCLKCSTNP 
      ISLLNHSEN GTWHTFEVV RTSPKQRDD YQAFFNNFY 
      LFCGQANVE ELRAKIIAR FQETISCSK SLCDQFLQF 
      VSSWSFREG EKEMLDKTT MQRVLVLFL LERSIQGFD 
      FKPVNQERK LLQEVISLF HVTLIGEDS CEKVKQIWG 
      DFGDEFENN GAEVAKIRA KYQIKSFDE LDSSDKAKI 
      LWLMGKCPL VIAADNGTM SKVLQLCPN EKFVLLTSH 
      ENVITLEGS FFRNLSDLD LSSDICQKI LRTFCCSIQ 
      GKDKRSLKC FVDRNKNFR ENVTVSDLI NMTDGPRLI 
      GEEKEKAPD PYITRFLTT NLINIKYLT TVDENTLVV 
      LDCMNKIDK IKRHLGNFN LHKVATYLK DLSLYNQDS 
      NQVTLFHSS PIIKQFVSG PDMYVSEAQ CTPVEFEEI 
      CQRNKNKEK CHHFRVIDE MTLEWIRSR KSITTLEQF 
      RIDGTYIKE SSLYGLEPE NNVNLVNGD PGMGKSELL 
      KNIKNNSNP EILCIFFPS KDTTLLCSQ LEGKSDLYE 
      AFVSFILNT KYRAFPSFD KKVIKFLID TDQVMYFWD 
      ALDEVSTKN MKTTIDLIE ILKGKSLCQ WVSSRCHLK 
      GHLEKKFNV LSRNINQFD EEEQSLYIH KRLGRLYSE 
      DDINKVIEK IRSAVTLMK HNDILGIPL QVFMLTELF 
      LQDKEKYFN LLKNMFSLA DLYHYIIEE KFNVYYKEK 
      AGISSPNDA LERILMFHR KNMYENYEK AALKASITK 
      EILKRFHIN CDNFLKELE TGDDNIGLI KEVTNSFPQ 
      FQHNSYAEY FVATYFIAH YEEIPELRS LLFDKRHAN 
      IRFFFDLLT AKDSPSLVA VLCKNIDLL KKHQEDLER 
      SEDKRGRNA LQLACSWGQ RYPILEVEK DNGTWILDN 
      TNDNTLCGE TPEYNEILQ YLLEHCNVT ETCKLFNLS 
      CVRYAEESD CLMTKLKIL LKQNNKFSK LKNDKEGIS 
      ILYYCTKFG YDEVIDLFE DLPFIRMKV NDFTLLHLA 
      VECEQEKYL RRLLQVPGY QKTIDKKDK FGRTPLYLA 
      CQLGLYKIV ELLIESGAN VNYSTPLHI ACLHAHDNI 
      VKLLLKAGT DINATDSYN ITPLHTASW NGHHTTVQI 
      LTANKNVEI DVQTSGGCT PLYVAARNK HDRVVELLI 
      EAKANVNLA QKENVTPLM IASQEGHQE NVKLLIQAG 
      SDVNQVATT GESPLYVAC QNGHDVVVE MLLEAGANT 
      EAFADNGWS PVFVACQQG HVGVVQLLM KAHANLGIK 
      DKTGRTPLM EKIVIKIKL KVPDRGKNV RLEQKILKT 
      CVSLLIMDL DDLLGPHYL NLNFSDDFL NESAPKLSI 
      ELPYPIHKP LKDLNLDII DEFHQNASL YSHKLSTLH 
      KDFRFARDK VMNQTFTDS HATITRGLL DFCNTEKPG 
      IQFPCDYNP YYTSGFLEY VILDNVEYL MRPDCDNNI 
      RLTKLAKPW KNRKIKVEV PFEDNITGI SFSSGSSCL 
      LVRHKSHIN LLKLTDLEG EIRQTKKFK KFLLDSQIC 
      EGQFGVVLS DTKFRLYDV ETNKRISLD DKTVDSVTR 
      CEFANEMII LLGRKKLKL FDKRSNDFE IFEPELQPC 
      NFFCSVKAH GDSLYVGSR HCLMRTDVR FLKNFDFCT 
      HLMKYGPCY MDLVEGFLC LSSQKVGEK VMFSGEPIS 
      SLPFKVPSI VETFSECRL KKDVLLVDG IKERVAKSV 
      GGVKIIHEN NDYFLYSAN SLGDIFKQK ITTNSDCDE 
      TKPVDSFHY WVNKLPEKP KPLHLTSVE NASLARFVL 
      NKGIDHEKS KKYSPRI
    CDS sequence
    • >TC007557 RA
      atggaaaac gaaattttt tgcaaacgc aagggatca 
      acggacaaa ggaaaactt tacgaagat ttagtcacc 
      gctgattta attctaaaa ttattgact gaacaaaat 
      gtaaataat tttaaactc tcatccaat aatgaagaa 
      ttcggagct ttcgacgat gtagtttta aaacttgat 
      actggggag atctacgcc ttgcaactg aaacactca 
      gataacaac gataaagct gtatgtgaa atagattta 
      acaggaggg aagaatttc aaacctcgg ttcgacctc 
      aaaaaatac tacgagtct tataaaaac atcaaagtg 
      ccttgtaaa gtcatactc ttcactaat cgaacctgc 
      gatattagt gaccaagaa tttcctttg aaccaaaac 
      accacaata aaatgttta aagtgcagc accaatcca 
      ataagctta ctgaaccat tctgaaaac ggcacttgg 
      cacactttt gaggtcgtt agaacttca cctaaacaa 
      cgtgacgat tatcaggcc ttcttcaac aatttttac 
      ctcttttgt ggacaagca aacgtcgaa gaacttaga 
      gctaaaatc atagcacga tttcaagaa accatctct 
      tgtagcaaa tcgctttgc gaccaattt ctccaattc 
      gtatcaagc tggagtttc cgcgaagga gagaaagaa 
      atgttggac aaaaccaca atgcaacgt gtgttagtt 
      cttttccta ctggaacgc tcaatccaa ggatttgac 
      tttaaaccc gtcaaccaa gagagaaaa ctgttacaa 
      gaagtgatc tctctgttt catgttaca ctcatcgga 
      gaggattct tgcgaaaaa gttaaacaa atttgggga 
      gattttggc gacgaattt gaaaataat ggagcagaa 
      gtggcaaaa atacgagcc aaatatcaa ataaaatca 
      ttcgatgaa ttagatagt agtgacaaa gcaaaaatt 
      ttgtggttg atgggaaag tgtcctcta gttattgca 
      gctgataat gggacaatg agtaaggta ttgcaactg 
      tgtcccaac gaaaaattt gttttgctc acaagccat 
      gagaatgtg atcacactg gagggttct ttttttcga 
      aatttatca gacttggat ttaagttca gatatttgc 
      caaaaaatt ctccgcacg ttttgctgc tccattcaa 
      ggaaaagac aagaggagt ttaaaatgt ttcgttgat 
      cggaataaa aattttcga gaaaacgta actgtcagt 
      gatttaata aatatgacg gatggtcct cgcctcatt 
      ggagaagaa aaagaaaaa gctccagac ccgtacatt 
      accagattt ttgacaact aatttaata aacataaaa 
      tatttaaca actgtcgat gaaaacaca ctcgtcgtt 
      cttgactgc atgaataaa attgataag atcaaaagg 
      cacttggga aattttaat ctgcacaaa gttgcaaca 
      tatctgaag gatctttcg ctttataac caggattca 
      aaccaagta acactgttc cattcaagc ccaatcata 
      aaacaattt gtttcgggt cccgatatg tacgtaagc 
      gaggcccaa tgtacaccg gtggaattt gaagagatt 
      tgtcaaaga aacaaaaac aaggagaag tgccaccac 
      ttcagagta atcgacgaa atgacatta gaatggata 
      aggagcaga aaaagcatc acaacttta gagcaattc 
      cgaattgat ggaacctac attaaagaa tcgagtctt 
      tacggctta gagccggaa aacaacgta aatttagtg 
      aacggagac cctggaatg ggaaaatcg gagttgctg 
      aaaaatatc aagaacaat tcaaatccg gagattctg 
      tgcatcttt ttcccttcg aaagataca actctactt 
      tgctcacag cttgaaggc aaaagtgac ttgtacgag 
      gcttttgtc agtttcatt cttaatacg aaatatcgg 
      gcgttcccg agtttcgat aaaaaagtc ataaagttt 
      ttaattgac acagatcaa gtgatgtac ttttgggac 
      gccttggac gaagtttcc acaaaaaat atgaagaca 
      actatagac cttattgag atattgaaa ggtaaaagt 
      ttgtgtcag tgggtgtcc agtcgctgc cacctcaag 
      ggtcatctg gagaagaaa ttcaacgtt ctttccaga 
      aacataaac cagtttgac gaagaggaa caatctctc 
      tacatacat aaacggctt ggacggtta tattcagag 
      gacgacatc aacaaagtg attgaaaaa atccgatcc 
      gctgtcact ttaatgaag cacaacgac attttgggc 
      atccctttg caagtgttt atgctaacc gagctcttc 
      cttcaagac aaggaaaag tacttcaat cttttgaaa 
      aacatgttt tctttagcc gacctctac cactacatc 
      atcgaggag aaattcaac gtctattac aaggagaaa 
      gccgggatt tcttcacct aacgacgct ttagagcga 
      atcctgatg ttccaccgc aaaaacatg tacgaaaat 
      tacgaaaaa gccgccttg aaggcctca atcactaag 
      gaaattctc aaacgtttc cacataaac tgcgacaat 
      tttttaaaa gagcttgaa accggcgac gacaacatt 
      ggcttgatc aaagaggtc actaacagt tttccccag 
      tttcagcac aattcctat gctgagtat ttcgtcgcc 
      acttacttt atcgcacat tatgaggaa atacccgag 
      ttgcggagc cttctgttc gacaagcga cacgcaaat 
      atccgattc ttcttcgac ctgctgacg gcgaaagac 
      tcgccttcg cttgtggca gttttgtgc aaaaacatc 
      gacttgttg aaaaaacac caagaggat ttggagcgc 
      agcgaggac aaacgaggc agaaacgcc ctacaactg 
      gcgtgctcg tggggacag aggtacccg attttagaa 
      gttgaaaag gataacggg acttggatt ttggacaac 
      accaatgat aacactctt tgtggcgaa acgccggag 
      tataacgaa attttgcag tatctttta gagcactgt 
      aacgtgacg gaaacttgc aagttgttt aatttgtcg 
      tgtgtgagg tatgccgaa gagagcgat tgtcttatg 
      acgaaactg aagattctc ttgaagcag aataacaag 
      ttttcgaaa ttgaagaac gacaaggaa gggatcagc 
      attctgtac tattgcacc aagtttggc tacgacgaa 
      gttatcgac ttgtttgaa gatttgccc tttattagg 
      atgaaagtc aacgatttt acgttatta cacctagct 
      gttgagtgc gaacaagaa aaatatctg agacgactc 
      cttcaagtt ccaggatat caaaaaacc atagataaa 
      aaagataaa ttcgggaga acaccactt tatttagct 
      tgtcagtta ggtttatac aaaattgtt gaattattg 
      atcgaaagt ggagccaat gttaattac tcaacacct 
      cttcatatc gcttgcctc cacgcccat gacaacatc 
      gtcaaattg ctcctgaaa gccggaaca gacataaac 
      gccaccgac agttacaac ataactcct ctgcacaca 
      gcttcctgg aacggccac cacaccact gtccaaatc 
      ctaactgca aacaaaaat gttgaaatc gatgttcaa 
      acatcaggt ggttgcact cctctctac gtggctgca 
      agaaacaag catgacaga gttgtggaa ttgttgatt 
      gaagcaaag gccaatgtt aatctagcg caaaaagaa 
      aatgttaca cctcttatg atagcatca caagaagga 
      caccaagaa aatgttaag ctgctgatt caagctggt 
      tctgatgtc aatcaagtg gcaacgacg ggggaatca 
      ccactttac gtggcttgc cagaatggt cacgatgtt 
      gtggttgaa atgcttcta gaagcggga gcaaatacc 
      gaagcgttt gcagacaat ggatggagt cctgttttt 
      gtagcgtgt cagcaagga catgttggg gttgttcag 
      ttgttaatg aaagctcat gcaaatttg ggtattaaa 
      gataagact ggaagaacc cctttgatg gaaaaaatt 
      gtaattaaa attaaatta aaggttcct gaccgcggc 
      aaaaatgta aggttagaa caaaaaatt ttgaagact 
      tgtgtgagc cttttaatt atggattta gacgacctg 
      ttaggcccc cattacttg aacttaaat ttcagtgac 
      gatttttta aatgaaagt gcaccaaaa ctctcaatt 
      gaactgcct tatcctatt cacaagccg ctgaaagac 
      ctaaacctt gacataatt gatgagttt caccaaaat 
      gcgagcctt tactctcac aaattatca accttacat 
      aaagatttt cgttttgcg agagataaa gtcatgaat 
      cagacgttt acagactca catgctaca attacaagg 
      gggttgtta gacttttgt aatacggaa aagcccgga 
      attcaattc ccctgcgat tacaaccct tactacacc 
      tcggggttt cttgaatat gttattttg gacaatgtg 
      gagtattta atgcgacca gattgcgac aataacatt 
      cggttgaca aaattggca aagccttgg aaaaataga 
      aaaatcaaa gttgaagtt ccatttgaa gataacata 
      actggaatc tcatttagt agtggttcc agttgttta 
      ttagtgaga cacaaaagt cacataaac ttgctcaaa 
      ctgaccgat cttgaggga gaaataaga caaacaaaa 
      aagttcaaa aagtttctc cttgattcc caaatctgc 
      gaaggacaa tttggagtc gttctctcc gacacaaaa 
      ttcagatta tacgacgtt gaaactaac aaaagaatt 
      agtctcgac gataaaact gttgactcc gtcactcgt 
      tgtgaattt gcgaatgag atgattatt cttttgggc 
      cggaaaaaa ctcaaactt tttgataaa cgttcaaat 
      gactttgaa atttttgag ccggaattg cagccttgt 
      aatttcttc tgctctgtt aaagcccac ggggactca 
      ctttatgtg ggctcaaga cattgttta atgcgaact 
      gatgtcagg tttttgaag aatttcgat ttttgcacg 
      catttgatg aaatacggg ccttgttac atggactta 
      gttgaaggt ttcttgtgt ctttcgagt cagaaagtt 
      ggggagaaa gttatgttc agtggagag cctattagt 
      agtttgcct tttaaagtc ccgagtatc gttgaaact 
      ttcagtgag tgtcggtta aaaaaggat gtattatta 
      gtggatggt attaaggaa agagttgca aaatcagtt 
      ggaggtgtt aaaataatt cacgaaaat aatgattat 
      ttcctctac agtgctaac agtttgggc gatattttt 
      aaacaaaaa atcacaacc aactcagac tgtgatgaa 
      acaaaacca gtggactcg tttcactat tgggtgaat 
      aaactacca gaaaagccc aagccgttg cacttgacc 
      agtgttgaa aatgcaagc ttggcaagg tttgttcta 
      aacaaaggt atcgaccat gagaaatcg aaaaaatac 
      agccctcga atttga
    mRNA sequence
    • >TC007557 RA
      tcaacacac tgaagtgcc aacaaaaat ttttatatt 
      aatttaaaa tggaaaacg aaatttttt gcaaacgca 
      agggatcaa cggacaaag gaaaacttt acgaagatt 
      tagtcaccg ctgatttaa ttctaaaat tattgactg 
      aacaaaatg taaataatt ttaaactct catccaata 
      atgaagaat tcggagctt tcgacgatg tagttttaa 
      aacttgata ctggggaga tctacgcct tgcaactga 
      aacactcag ataacaacg ataaagctg tatgtgaaa 
      tagatttaa caggaggga agaatttca aacctcggt 
      tcgacctca aaaaatact acgagtctt ataaaaaca 
      tcaaagtgc cttgtaaag tcatactct tcactaatc 
      gaacctgcg atattagtg accaagaat ttcctttga 
      accaaaaca ccacaataa aatgtttaa agtgcagca 
      ccaatccaa taagcttac tgaaccatt ctgaaaacg 
      gcacttggc acacttttg aggtcgtta gaacttcac 
      ctaaacaac gtgacgatt atcaggcct tcttcaaca 
      atttttacc tcttttgtg gacaagcaa acgtcgaag 
      aacttagag ctaaaatca tagcacgat ttcaagaaa 
      ccatctctt gtagcaaat cgctttgcg accaatttc 
      tccaattcg tatcaagct ggagtttcc gcgaaggag 
      agaaagaaa tgttggaca aaaccacaa tgcaacgtg 
      tgttagttc ttttcctac tggaacgct caatccaag 
      gatttgact ttaaacccg tcaaccaag agagaaaac 
      tgttacaag aagtgatct ctctgtttc atgttacac 
      tcatcggag aggattctt gcgaaaaag ttaaacaaa 
      tttggggag attttggcg acgaatttg aaaataatg 
      gagcagaag tggcaaaaa tacgagcca aatatcaaa 
      taaaatcat tcgatgaat tagatagta gtgacaaag 
      caaaaattt tgtggttga tgggaaagt gtcctctag 
      ttattgcag ctgataatg ggacaatga gtaaggtat 
      tgcaactgt gtcccaacg aaaaatttg ttttgctca 
      caagccatg agaatgtga tcacactgg agggttctt 
      tttttcgaa atttatcag acttggatt taagttcag 
      atatttgcc aaaaaattc tccgcacgt tttgctgct 
      ccattcaag gaaaagaca agaggagtt taaaatgtt 
      tcgttgatc ggaataaaa attttcgag aaaacgtaa 
      ctgtcagtg atttaataa atatgacgg atggtcctc 
      gcctcattg gagaagaaa aagaaaaag ctccagacc 
      cgtacatta ccagatttt tgacaacta atttaataa 
      acataaaat atttaacaa ctgtcgatg aaaacacac 
      tcgtcgttc ttgactgca tgaataaaa ttgataaga 
      tcaaaaggc acttgggaa attttaatc tgcacaaag 
      ttgcaacat atctgaagg atctttcgc tttataacc 
      aggattcaa accaagtaa cactgttcc attcaagcc 
      caatcataa aacaatttg tttcgggtc ccgatatgt 
      acgtaagcg aggcccaat gtacaccgg tggaatttg 
      aagagattt gtcaaagaa acaaaaaca aggagaagt 
      gccaccact tcagagtaa tcgacgaaa tgacattag 
      aatggataa ggagcagaa aaagcatca caactttag 
      agcaattcc gaattgatg gaacctaca ttaaagaat 
      cgagtcttt acggcttag agccggaaa acaacgtaa 
      atttagtga acggagacc ctggaatgg gaaaatcgg 
      agttgctga aaaatatca agaacaatt caaatccgg 
      agattctgt gcatctttt tcccttcga aagatacaa 
      ctctacttt gctcacagc ttgaaggca aaagtgact 
      tgtacgagg cttttgtca gtttcattc ttaatacga 
      aatatcggg cgttcccga gtttcgata aaaaagtca 
      taaagtttt taattgaca cagatcaag tgatgtact 
      tttgggacg ccttggacg aagtttcca caaaaaata 
      tgaagacaa ctatagacc ttattgaga tattgaaag 
      gtaaaagtt tgtgtcagt gggtgtcca gtcgctgcc 
      acctcaagg gtcatctgg agaagaaat tcaacgttc 
      tttccagaa acataaacc agtttgacg aagaggaac 
      aatctctct acatacata aacggcttg gacggttat 
      attcagagg acgacatca acaaagtga ttgaaaaaa 
      tccgatccg ctgtcactt taatgaagc acaacgaca 
      ttttgggca tccctttgc aagtgttta tgctaaccg 
      agctcttcc ttcaagaca aggaaaagt acttcaatc 
      ttttgaaaa acatgtttt ctttagccg acctctacc 
      actacatca tcgaggaga aattcaacg tctattaca 
      aggagaaag ccgggattt cttcaccta acgacgctt 
      tagagcgaa tcctgatgt tccaccgca aaaacatgt 
      acgaaaatt acgaaaaag ccgccttga aggcctcaa 
      tcactaagg aaattctca aacgtttcc acataaact 
      gcgacaatt ttttaaaag agcttgaaa ccggcgacg 
      acaacattg gcttgatca aagaggtca ctaacagtt 
      ttccccagt ttcagcaca attcctatg ctgagtatt 
      tcgtcgcca cttacttta tcgcacatt atgaggaaa 
      tacccgagt tgcggagcc ttctgttcg acaagcgac 
      acgcaaata tccgattct tcttcgacc tgctgacgg 
      cgaaagact cgccttcgc ttgtggcag ttttgtgca 
      aaaacatcg acttgttga aaaaacacc aagaggatt 
      tggagcgca gcgaggaca aacgaggca gaaacgccc 
      tacaactgg cgtgctcgt ggggacaga ggtacccga 
      ttttagaag ttgaaaagg ataacggga cttggattt 
      tggacaaca ccaatgata acactcttt gtggcgaaa 
      cgccggagt ataacgaaa ttttgcagt atcttttag 
      agcactgta acgtgacgg aaacttgca agttgttta 
      atttgtcgt gtgtgaggt atgccgaag agagcgatt 
      gtcttatga cgaaactga agattctct tgaagcaga 
      ataacaagt tttcgaaat tgaagaacg acaaggaag 
      ggatcagca ttctgtact attgcacca agtttggct 
      acgacgaag ttatcgact tgtttgaag atttgccct 
      ttattagga tgaaagtca acgatttta cgttattac 
      acctagctg ttgagtgcg aacaagaaa aatatctga 
      gacgactcc ttcaagttc caggatatc aaaaaacca 
      tagataaaa aagataaat tcgggagaa caccacttt 
      atttagctt gtcagttag gtttataca aaattgttg 
      aattattga tcgaaagtg gagccaatg ttaattact 
      caacacctc ttcatatcg cttgcctcc acgcccatg 
      acaacatcg tcaaattgc tcctgaaag ccggaacag 
      acataaacg ccaccgaca gttacaaca taactcctc 
      tgcacacag cttcctgga acggccacc acaccactg 
      tccaaatcc taactgcaa acaaaaatg ttgaaatcg 
      atgttcaaa catcaggtg gttgcactc ctctctacg 
      tggctgcaa gaaacaagc atgacagag ttgtggaat 
      tgttgattg aagcaaagg ccaatgtta atctagcgc 
      aaaaagaaa atgttacac ctcttatga tagcatcac 
      aagaaggac accaagaaa atgttaagc tgctgattc 
      aagctggtt ctgatgtca atcaagtgg caacgacgg 
      gggaatcac cactttacg tggcttgcc agaatggtc 
      acgatgttg tggttgaaa tgcttctag aagcgggag 
      caaataccg aagcgtttg cagacaatg gatggagtc 
      ctgtttttg tagcgtgtc agcaaggac atgttgggg 
      ttgttcagt tgttaatga aagctcatg caaatttgg 
      gtattaaag ataagactg gaagaaccc ctttgatgg 
      aaaaaattg taattaaaa ttaaattaa aggttcctg 
      accgcggca aaaatgtaa ggttagaac aaaaaattt 
      tgaagactt gtgtgagcc ttttaatta tggatttag 
      acgacctgt taggccccc attacttga acttaaatt 
      tcagtgacg attttttaa atgaaagtg caccaaaac 
      tctcaattg aactgcctt atcctattc acaagccgc 
      tgaaagacc taaaccttg acataattg atgagtttc 
      accaaaatg cgagccttt actctcaca aattatcaa 
      ccttacata aagattttc gttttgcga gagataaag 
      tcatgaatc agacgttta cagactcac atgctacaa 
      ttacaaggg ggttgttag acttttgta atacggaaa 
      agcccggaa ttcaattcc cctgcgatt acaaccctt 
      actacacct cggggtttc ttgaatatg ttattttgg 
      acaatgtgg agtatttaa tgcgaccag attgcgaca 
      ataacattc ggttgacaa aattggcaa agccttgga 
      aaaatagaa aaatcaaag ttgaagttc catttgaag 
      ataacataa ctggaatct catttagta gtggttcca 
      gttgtttat tagtgagac acaaaagtc acataaact 
      tgctcaaac tgaccgatc ttgagggag aaataagac 
      aaacaaaaa agttcaaaa agtttctcc ttgattccc 
      aaatctgcg aaggacaat ttggagtcg ttctctccg 
      acacaaaat tcagattat acgacgttg aaactaaca 
      aaagaatta gtctcgacg ataaaactg ttgactccg 
      tcactcgtt gtgaatttg cgaatgaga tgattattc 
      ttttgggcc ggaaaaaac tcaaacttt ttgataaac 
      gttcaaatg actttgaaa tttttgagc cggaattgc 
      agccttgta atttcttct gctctgtta aagcccacg 
      gggactcac tttatgtgg gctcaagac attgtttaa 
      tgcgaactg atgtcaggt ttttgaaga atttcgatt 
      tttgcacgc atttgatga aatacgggc cttgttaca 
      tggacttag ttgaaggtt tcttgtgtc tttcgagtc 
      agaaagttg gggagaaag ttatgttca gtggagagc 
      ctattagta gtttgcctt ttaaagtcc cgagtatcg 
      ttgaaactt tcagtgagt gtcggttaa aaaaggatg 
      tattattag tggatggta ttaaggaaa gagttgcaa 
      aatcagttg gaggtgtta aaataattc acgaaaata 
      atgattatt tcctctaca gtgctaaca gtttgggcg 
      atattttta aacaaaaaa tcacaacca actcagact 
      gtgatgaaa caaaaccag tggactcgt ttcactatt 
      gggtgaata aactaccag aaaagccca agccgttgc 
      acttgacca gtgttgaaa atgcaagct tggcaaggt 
      ttgttctaa acaaaggta tcgaccatg agaaatcga 
      aaaaataca gccctcgaa tttgagcga gttagctca 
      aaatttctt atcggtttg ggacgacga agacgagga 
      ggagcgcct tgaagtgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY