Status
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Gene information
General information
TC007573 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2910104..2912502
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007573
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09864

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007573 RA
      MGANISQLE RDIGSEQFP SNEHYFGLV NFGNTCYSN 
      SVLQALYFC KPFRDRVLE YKAKNKRTK ETLLTCLAD 
      LFHSIATQK KKVGSIAPK KFIARLRKE KEEFDNYMQ 
      QDAHEFLNF LINHISEII LAERQQNST TNNIKSKAT 
      GENGTAHTT PEPTWVHEI FQGILTSET RCLNCENVS 
      SKDEDFFDL QVDIEQNTS ITHCLRCFS NTETLCSDN 
      KFKCDNCSS YQEAQKRMR VKKLPMILA LHLKRFKYM 
      EQYNRHIKV SHRVVFPLE LRLFNTSDD AVNPDRLYD 
      LVAVVIHCG SGPNRGHYI SIVKSHGFW LLFDDDQVD 
      KIDASTIED FYGLTSDTQ KSSETGYIL FYQSRESL
    CDS sequence
    • >TC007573 RA
      atgggtgcg aatatatcc cagctggaa cgagatatc 
      ggctctgaa cagtttcct tctaatgaa cattacttt 
      ggtttagta aattttgga aatacgtgt tatagtaac 
      tcggttctt caggctcta tacttctgc aaacctttt 
      agagataga gttttagag tacaaagcg aaaaataag 
      aggactaaa gaaactttg cttacatgt cttgcagat 
      ttgttccac agcattgcg acgcagaaa aagaaagta 
      ggctctatt gcaccaaaa aaatttata gcaaggcta 
      cgaaaagaa aaagaggag tttgataat tacatgcaa 
      caggatgca catgaattt ttaaatttt ttaatcaac 
      catattagt gaaataatt ctggctgaa agacaacaa 
      aactcaacc acaaacaac ataaaatca aaagcaacg 
      ggagaaaat ggaactgca cacacaact cctgagccc 
      acttgggta cacgagatt tttcagggt atattgaca 
      agtgaaact aggtgtttg aattgtgag aatgtgagc 
      agtaaagat gaagacttt tttgattta caagtggac 
      atcgaacag aacactagt ataactcat tgtttgagg 
      tgttttagt aatacagag actctgtgt agcgataat 
      aaatttaag tgtgataac tgcagtagt taccaggaa 
      gctcaaaaa cgaatgagg gtgaagaaa ctgccgatg 
      attcttgca ttgcatctc aagaggttc aagtacatg 
      gaacagtac aacaggcac attaaggtc tcacacaga 
      gttgtgttt cctttggaa ctgagactt ttcaacaca 
      tcggacgat gcagttaat cccgaccga ttatacgat 
      ttagtagct gttgtgata cattgcgga agcggacct 
      aacagaggc cactacatc agtatagtc aagagccac 
      ggtttctgg ctgctgttt gacgacgac caagttgac 
      aaaatagac gcttcaaca atagaagat ttctacggc 
      ctcacgtct gatactcaa aagtcttca gaaacaggc 
      tacatcctt ttttaccag tctcgagag agtttatga
    mRNA sequence
    • >TC007573 RA
      gttgtttta cagctgtca aaatgcaat aatggggaa 
      gttttgtga tttaaaagt attgttttt ggacgtttt 
      taacataaa cgtgatgga ttagttatt aaaatatgg 
      gtgcgaata tatcccagc tggaacgag atatcggct 
      ctgaacagt ttccttcta atgaacatt actttggtt 
      tagtaaatt ttggaaata cgtgttata gtaactcgg 
      ttcttcagg ctctatact tctgcaaac cttttagag 
      atagagttt tagagtaca aagcgaaaa ataagagga 
      ctaaagaaa ctttgctta catgtcttg cagatttgt 
      tccacagca ttgcgacgc agaaaaaga aagtaggct 
      ctattgcac caaaaaaat ttatagcaa ggctacgaa 
      aagaaaaag aggagtttg ataattaca tgcaacagg 
      atgcacatg aatttttaa attttttaa tcaaccata 
      ttagtgaaa taattctgg ctgaaagac aacaaaact 
      caaccacaa acaacataa aatcaaaag caacgggag 
      aaaatggaa ctgcacaca caactcctg agcccactt 
      gggtacacg agatttttc agggtatat tgacaagtg 
      aaactaggt gtttgaatt gtgagaatg tgagcagta 
      aagatgaag acttttttg atttacaag tggacatcg 
      aacagaaca ctagtataa ctcattgtt tgaggtgtt 
      ttagtaata cagagactc tgtgtagcg ataataaat 
      ttaagtgtg ataactgca gtagttacc aggaagctc 
      aaaaacgaa tgagggtga agaaactgc cgatgattc 
      ttgcattgc atctcaaga ggttcaagt acatggaac 
      agtacaaca ggcacatta aggtctcac acagagttg 
      tgtttcctt tggaactga gacttttca acacatcgg 
      acgatgcag ttaatcccg accgattat acgatttag 
      tagctgttg tgatacatt gcggaagcg gacctaaca 
      gaggccact acatcagta tagtcaaga gccacggtt 
      tctggctgc tgtttgacg acgaccaag ttgacaaaa 
      tagacgctt caacaatag aagatttct acggcctca 
      cgtctgata ctcaaaagt cttcagaaa caggctaca 
      tcctttttt accagtctc gagagagtt tatgaactc 
      tgctgctcc ttgccttaa tcattgaga ggatttaat 
      acaagtaca aagtcacct ctcattgca ttgggacgg 
      gtaatgttt atgaaaaaa gtctgtaaa tatgatttg 
      aaactgaaa attgttgga tttatattt tatttattg 
      catattgta gtttccggc cgtactttt ttcctaaaa 
      tttttcttt caaagcgat ttacccgta cttttcgaa 
      acgaatcga cgttgattt tgttgttct gttgcaata 
      aatagcgat atgttgaaa acaagattt actttattg 
      tggtggaac agcatagga ataattgac tgtatggtg 
      aattttact tgtttactt aatttgttt tttacaaac 
      cttatattt attccaatt agcaataat tcttaggtg 
      gtaaccaaa ccgtgcatc taatttagc gagtattat 
      gtcccctac acggtaaaa aagtaaaat tcactcttc 
      ttattttgt taacaaata ctatacaac ttttaaata 
      ttatatgag aaactagaa catactgta taaatgctg 
      ctaagcaga ttttatttt tattctgta aatattcgt 
      tagatataa atatatttg aaaaaatgc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY