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Gene information
General information
TC007914 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2633043..2635997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007914
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09859

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007914 RA
      MKPSANFLL IVVCVLPNC AADFWEYFI GPRLSTGNG 
      SERAAPKVT DQLVRSYGY RLDTHLVAS QTGHILTLH 
      RIPRGRKAA GTKPRPVAF IHHGLFGCS DMWLSRGPH 
      LDLPYILAD SGYDVWLFN TRGNVYSRK HKSLDPDRD 
      AEYWNFGIE EMGYYDLPV TIDYILNIT NQKDLFYLG 
      HSIGSSTGF ITCSLRPEY NSKIRLFMA LGPLAHIRH 
      PLNLLHKVL FSLLSPALS LVESMNIYE IWPRRFHIS 
      RLVEAACED GSPFQKLCL MLIFSVVGE DPTQLNTTT 
      FPNFVQYYP AGTSLKVVS NIVQYYVSG EFARFSGGK 
      TVPFIYDLA KVTAPVALY YGPGDLLVT QEDVDYLSH 
      RLGNVTGKF RIPYKHFNH LDFVLANNA RSLLYNNLL 
      SVMEKYK
    CDS sequence
    • >TC007914 RA
      atgaaaccg agcgcgaat ttcctactg attgtggtg 
      tgtgttttg ccaaattgc gcggccgat ttttgggaa 
      tacttcatc gggccccgg ttatccaca gggaatggc 
      agcgaacga gcggcgcca aaagttacg gatcagctt 
      gtgagaagc tacggttac cgtttggac acgcatctg 
      gtggcgtca cagacgggg cacattttg acactccac 
      cgaatacca agaggtcga aaggcagcg ggtactaag 
      cccagacca gttgccttc atccatcac ggcttgttc 
      ggctgctca gatatgtgg ctttcgaga ggaccgcac 
      ttggaccta ccctacatt ttggcagat tcgggctac 
      gacgtgtgg ctgtttaac acaagaggc aacgtttat 
      tcgcgcaaa cataaatct ctagatccg gaccgcgat 
      gccgaatac tggaatttt gggatcgaa gagatgggt 
      tactacgat ttgccggtc actatcgac tacattttg 
      aatataaca aaccagaag gatttgttt tatttgggt 
      cactctatc ggcagttcg actggattt attacttgc 
      tcgttacgg ccagagtat aacagcaaa attcgcctg 
      tttatggcc ttaggtcct ctagcgcac atcagacat 
      ccgctcaat ttattgcac aaagtttta ttctccttg 
      ctatcgccg gctttgtct ctggtggag tcaatgaac 
      atttacgaa atctggccg aggaggttc cacataagt 
      aggctggtg gaggcggct tgtgaggac ggctccccc 
      tttcagaag ctgtgtctg atgttgatt ttttccgtt 
      gttggagag gacccgact cagttgaat acaactacg 
      tttcctaat ttcgttcag tattatccg gccgggact 
      tcgctcaaa gtcgtgtcg aacatcgtt cagtattac 
      gtcagtggc gaatttgcg aggttttca ggcggtaaa 
      acagttcct tttatttat gatttagcg aaagtcacg 
      gccccggtg gccctctat tacggccct ggcgacttg 
      cttgtgacg caagaagat gtggattat ttgagccac 
      cggctgggg aatgtgacg gggaaattt cgaattccg 
      tacaaacac ttcaaccat ttggacttc gtgttggcc 
      aacaatgcg cgcagtttg ttgtataat aatttgttg 
      agtgtgatg gaaaagtat aaataa
    mRNA sequence
    • >TC007914 RA
      cgctaaaga ccagtgcgg cgcttgcaa aacgttttt 
      cacaattcg taaagtcga tcatcaatt atgtgcgtc 
      aatatgcat aaaatgata tattaataa ataacatgc 
      ggaaaagat ttagtaatc cctaagcgt gaagctcat 
      actctcctt aaaacgagc cttgtgtct gaccaaaca 
      catttaaac tttctcacc acgaacatt actgaaata 
      acaaaaccg agcttatga catgtcaaa ccgaaaatg 
      aaaccgagc gcgaatttc ctactgatt gtggtgtgt 
      gttttgcca aattgcgcg gccgatttt tgggaatac 
      ttcatcggg ccccggtta tccacaggg aatggcagc 
      gaacgagcg gcgccaaaa gttacggat cagcttgtg 
      agaagctac ggttaccgt ttggacacg catctggtg 
      gcgtcacag acggggcac attttgaca ctccaccga 
      ataccaaga ggtcgaaag gcagcgggt actaagccc 
      agaccagtt gccttcatc catcacggc ttgttcggc 
      tgctcagat atgtggctt tcgagagga ccgcacttg 
      gacctaccc tacattttg gcagattcg ggctacgac 
      gtgtggctg tttaacaca agaggcaac gtttattcg 
      cgcaaacat aaatctcta gatccggac cgcgatgcc 
      gaatactgg aattttggg atcgaagag atgggttac 
      tacgatttg ccggtcact atcgactac attttgaat 
      ataacaaac cagaaggat ttgttttat ttgggtcac 
      tctatcggc agttcgact ggatttatt acttgctcg 
      ttacggcca gagtataac agcaaaatt cgcctgttt 
      atggcctta ggtcctcta gcgcacatc agacatccg 
      ctcaattta ttgcacaaa gttttattc tccttgcta 
      tcgccggct ttgtctctg gtggagtca atgaacatt 
      tacgaaatc tggccgagg aggttccac ataagtagg 
      ctggtggag gcggcttgt gaggacggc tcccccttt 
      cagaagctg tgtctgatg ttgattttt tccgttgtt 
      ggagaggac ccgactcag ttgaataca actacgttt 
      cctaatttc gttcagtat tatccggcc gggacttcg 
      ctcaaagtc gtgtcgaac atcgttcag tattacgtc 
      agtggcgaa tttgcgagg ttttcaggc ggtaaaaca 
      gttcctttt atttatgat ttagcgaaa gtcacggcc 
      ccggtggcc ctctattac ggccctggc gacttgctt 
      gtgacgcaa gaagatgtg gattatttg agccaccgg 
      ctggggaat gtgacgggg aaatttcga attccgtac 
      aaacacttc aaccatttg gacttcgtg ttggccaac 
      aatgcgcgc agtttgttg tataataat ttgttgagt 
      gtgatggaa aagtataaa taaaacgct gaacaaaac 
      acatttatt tttaatatt gtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY