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Gene information
General information
TC032762 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2207712..2210069
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032762
    iBeetle screen iB_09854

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032762 RA
      MPARIAPSL PILLLFLTL GHSYRILGL FPHPAKSHV 
      NVFYPLMKG LALKGHDVT FVSHYKFND NIPTLKEMQ 
      IVNPGADLV NAIDMSQLL GNRLQMYLN NVLLAYWAV 
      NTCHDGLST PVFQELLNS DESYDLVIM EFFNTDCYA 
      PFAYKFKAH LIGLSSCTI MHWTNERFA NIYNPSYIP 
      ITHMDYSDR MNFFERVEN TALGVLHEI LYNYILRFN 
      DERIARMYF KEDFPSLTK IVHNASVFL VNTHFSLNL 
      PKPAVPAVI DIGGIHIGN VKKVPQVGI SQF
    CDS sequence
    • >TC032762 RA
      atgccggcg cgaattgct ccatcttta ccaattctc 
      ttattgttc ctcactctg ggccattcc taccgaatt 
      ctgggctta tttccccac ccagccaag agtcatgtg 
      aatgtgttc tatcccttg atgaagggc ctggcatta 
      aaaggccac gacgtgacg ttcgtaagt cattataaa 
      tttaacgac aacatcccg acactcaaa gaaatgcaa 
      atcgtaaac ccgggagca gatttggta aatgccatt 
      gacatgtcc caactgctg ggtaaccgg cttcagatg 
      tacctaaac aacgtactt ctggcatac tgggctgtg 
      aacacctgc catgacggg ctgtccacg cccgtgttc 
      caggagctg ctcaacagt gacgaatct tacgattta 
      gtcatcatg gagttcttc aatacggat tgttacgca 
      ccgttcgct tacaaattc aaagcgcat ttgatcggc 
      ctcagctcc tgcacgatc atgcattgg actaatgaa 
      agatttgcc aatatttac aatccgtct tatattccc 
      atcactcat atggattac agtgaccga atgaacttc 
      tttgagcga gtcgagaat acggctctc ggagtgctc 
      cacgaaatt ctctacaat tatattttg agatttaac 
      gatgaaaga atcgcgaga atgtacttc aaagaggat 
      tttccctcg cttacgaaa attgtacac aatgcaagt 
      gtcttccta gtcaatact catttcagt ctgaattta 
      ccaaaacct gcagttcct gccgttatc gacattgga 
      ggcatacac atcggaaac gtgaagaag gtgccgcag 
      gtgggtatt tcacaattt tag
    mRNA sequence
    • >TC032762 RA
      ctcggttat tattaccat cagtgagag gttattcct 
      ctagtttac atcaattac atgacgtag cagtagtgc 
      aacttcgta gattcacga gtgaatatc gatagtgtt 
      ttcgtgtac aacaaaccg agttgtttc cgtgttaca 
      taaccggca gataaaatg ccggcgcga attgctcca 
      tctttacca attctctta ttgttcctc actctgggc 
      cattcctac cgaattctg ggcttattt ccccaccca 
      gccaagagt catgtgaat gtgttctat cccttgatg 
      aagggcctg gcattaaaa ggccacgac gtgacgttc 
      gtaagtcat tataaattt aacgacaac atcccgaca 
      ctcaaagaa atgcaaatc gtaaacccg ggagcagat 
      ttggtaaat gccattgac atgtcccaa ctgctgggt 
      aaccggctt cagatgtac ctaaacaac gtacttctg 
      gcatactgg gctgtgaac acctgccat gacgggctg 
      tccacgccc gtgttccag gagctgctc aacagtgac 
      gaatcttac gatttagtc atcatggag ttcttcaat 
      acggattgt tacgcaccg ttcgcttac aaattcaaa 
      gcgcatttg atcggcctc agctcctgc acgatcatg 
      cattggact aatgaaaga tttgccaat atttacaat 
      ccgtcttat attcccatc actcatatg gattacagt 
      gaccgaatg aacttcttt gagcgagtc gagaatacg 
      gctctcgga gtgctccac gaaattctc tacaattat 
      attttgaga tttaacgat gaaagaatc gcgagaatg 
      tacttcaaa gaggatttt ccctcgctt acgaaaatt 
      gtacacaat gcaagtgtc ttcctagtc aatactcat 
      ttcagtctg aatttacca aaacctgca gttcctgcc 
      gttatcgac attggaggc atacacatc ggaaacgtg 
      aagaaggtg ccgcaggtg ggtatttca caattttag 
      ccgtaaata aaacgttag aagtatgta agtgtgaat 
      cgatagttt cgagaagct taaatacct gatgtttac 
      caggtcaag gatatattt caaccaaaa attttagtg 
      ataagaaaa ataaacatt gatcagtga tcacttcga 
      gcatgactt gacgaatcg agtgatagt gatacgaat 
      acttgttaa tgaataagc atttgggat caaatgtga 
      tacgtcagt gtaacgtta ctcgcagaa attatgctc 
      taataaatt tctcgagtt cgataggca attgcaaaa 
      ctagtaaaa acttatctc aagttgcat gattgtctc 
      aaagttgtt atcagaaaa cctaattcg gaatcgcta 
      cctttgatg ccacaaaca gtgaccctt tatctcaag 
      aaggcacac aaacagtaa aaataaatc attggctcg 
      ggtccaacc gtagtcgtt tgttagcag tcgcgcaat 
      atgtctctg cacgcgtta atagtgtta tcaaattta 
      ttcctcttg gtccattct tacaaaatc ctggcgttg 
      tttccccat cccgggaaa agtcacgta gacgtcttt 
      cttccttta acgaaagct ctagcaaga aaaggtcac 
      gaagttacc gtgatcagt cacttcccg ttgaaaaca 
      ccacaaatc aactacacg gacgtgagt ctcggcaac 
      gaaaccacc cctttaatt aacatattg gatatggac 
      aataaagcg cggacgaga tgggagaag tggggcatt 
      atacaccta ctgcaggaa ttcgcagcg tattcctgc 
      aaaattgga ttcgaatcg tcgcaattg cgagagttt 
      ctcagacat gagcgcact ttcgacgtg atcattgtc 
      gaatttttc aatagtgat tgtttcgct gggcttgtg 
      cagaaattc aaagcgccg gtggtcggg attagctcc 
      tgcaccatc atgcactgg actaatgag aggttcggg 
      aatcccaca catccggcc tacattccg aataatctc 
      atggagttt agcgaccga atgacgttt tttcagcga 
      attgaaaac ttgttaagc ggactgttt catcatttt 
      ttttacaac aaaatcata atgaacacg gatgaaacg 
      ctgattcgg aaatatctc ggatatgaa acaccaacg 
      ctcaaacaa attgttttt aatgccagt ttattgctg 
      gtaaacaca catttcagt ttaaatctg ccgagaccc 
      ctagttcct gctgtgatc gaagtcggg gggattcac 
      attgataag ccgaaaaaa ataccagaa gtaagtaaa 
      gtctctcat tcacgcgct cgcccgccc cgttataaa 
      taaaaaata gttggtagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY