Status
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Gene information
General information
TC007651 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1580055..1586241
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007651
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09848

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages potentially partiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax potentially partiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Potentially mixed identity
    abdomen potentially partiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Potentially mixed identity
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007651 RA
      MPGVADMGM VWMGAGQPN PDMSMELMS GDNSDMLLE 
      HENPYFTPS IHSMSNQSM DDIHTIHSS QMDAMENLD 
      MMQYIDMKH ESNDSSYAE DDSQNPVYM INSATQTLP 
      CSTRKIKPI KHPGLVLKT PIAYQSNTD PSVIPIQKD 
      GIAVCEKCG AIGVKHAFY TRERRFCSM ACARGYSGL 
      IPEPLPLSQ QQTPEHHHF AKHRFTMKT EDDFANTVV 
      DQEFPQLPP PPVLPPIDE TVPLIRRKP SELANSHDW 
      DSQLNDQYF VAAPVTCFK HAPMSDIWE NIMVGMKVE 
      VENTDCDNV SEAFPDSFW VATVLKIVG YKALLRYEG 
      FGSNDSKDF WVSLCSNQV HPVGWCATR GKPLIPPKT 
      IEDKYNDWK DFLSKRLTG ARTLPSNFS NKASDSLKS 
      RFQCGLNLE VVDKNRISQ VKVAIIHKI VGKRLNVKY 
      FNMPSDDAG FWCHEDSPL LHPVGWAKK VGHHLVAPV 
      NYLERVNHC IYDDDDATD ELFTPFQVG SKEPTADGG 
      FCIGMKLEA IDPLNLSSI CVATVMNVL RHGYIMIRI 
      DTYESDMTG ADWFCYHVK SPCIFPVGF CEKYDIPLT 
      PPKGYDQDT FTWRNYLLE TNNVAASPD LFASYIPMH 
      GFVPGMKIE AADLMDPRL VCVATIAKV AGRLLKVHF 
      DGWEEEYDQ WLDCESPDI YPVGWCQSV GHKLEGPPV 
      IPPKPTTPV AKLAKGMKK RGRKKKIKE VSPKAATKV 
      IKAEVEPEI EREEMEVAD DNQGPEAGQ EPAGPDQSL 
      PVATDANPP PERKATSYI NATNPTSTK LIPRLVDST 
      GTSSETGEL CPDEWNIYD VAQFLRVND CANYCDSFS 
      KQKVDGKTL MNLSKEDIL EYTGGKVGP SLKIFDLIQ 
      QLKIKVNPA QIRHMKANI KKFL
    CDS sequence
    • >TC007651 RA
      atgcccgga gtggccgac atgggcatg gtctggatg 
      ggtgctggg cagcccaac cccgacatg tccatggaa 
      ctcatgtcc ggggacaac tcggacatg ctgctggag 
      cacgagaac ccatatttc accccatcc atacacagc 
      atgtccaac cagtccatg gacgatatc cacactatc 
      cacagctcg caaatggac gctatggaa aacctagat 
      atgatgcaa tatatcgac atgaagcac gaaagtaac 
      gactcaagt tatgccgaa gatgatagt cagaaccct 
      gtatatatg ataaactct gccacgcaa acattgccc 
      tgctctacg cggaaaatc aaaccaatc aaacaccct 
      gggttggtt ttgaaaaca ccaatagcc taccaaagc 
      aatacggac ccctcagtc ataccaatt caaaaagat 
      ggaattgct gtttgcgag aagtgcggc gccatcggc 
      gtcaagcac gccttttac acccgagag cgccgcttc 
      tgcagcatg gcttgcgcc cgcggctac agcggcctg 
      atccccgag cccttgccc ctgtcccag cagcaaacc 
      cccgaacac caccatttc gccaaacac cgcttcacc 
      atgaaaacc gaagacgac tttgccaac acagtcgtc 
      gaccaggaa ttcccccag ctgccccca ccgccagtg 
      ctgcccccc atcgacgaa acagtcccg ctaatccgt 
      cgcaagccc tccgaactg gcgaactcg cacgactgg 
      gattcgcaa ttaaacgac cagtatttc gtggcagcc 
      cctgtcacg tgtttcaaa cacgcgccc atgtccgac 
      atttgggag aatatcatg gtcgggatg aaagtcgaa 
      gtggaaaat accgactgt gataacgtc tcagaggcg 
      tttcccgac tcgttttgg gtggctact gttttaaaa 
      atcgtgggg tataaggcg ttgctgcgg tacgagggg 
      ttcgggtcg aatgattcc aaagatttt tgggtctcg 
      ttgtgttcg aatcaggtg catccggtc gggtggtgc 
      gccacgagg gggaagccc ctgattccg ccgaaaacg 
      atcgaggac aagtataac gattggaag gattttttg 
      agcaagagg ttgacgggg gcacgcacg ttaccgtcg 
      aattttagc aataaagcg agtgatagt cttaagtct 
      agatttcaa tgcggcctt aatttagaa gttgtcgac 
      aaaaataga atttcgcaa gttaaggtt gccattatt 
      cataaaatc gtggggaag aggctgaat gtgaagtat 
      tttaatatg ccgtcggat gatgcgggg ttttggtgt 
      cacgaggac tcgccgctt cttcatccc gttggctgg 
      gcaaagaag gtcggccac cacctagtg gcccctgtc 
      aactacctg gagcgcgtc aaccactgc atttacgac 
      gacgacgac gccactgac gagcttttc acccctttc 
      caagtcggt tcaaaagaa cccactgcc gacggcggc 
      ttctgcatc ggaatgaaa ctcgaagca atcgatcct 
      ctcaacctg tcctcaata tgcgtcgcc accgtaatg 
      aacgtccta cgccacggc tacatcatg atccggata 
      gacacctac gaatcggat atgaccgga gccgattgg 
      ttctgctac cacgtcaaa tccccgtgc attttcccc 
      gtcggtttt tgcgaaaaa tacgacatt cccctaacc 
      cctccaaaa ggctacgac caggacacc ttcacctgg 
      cggaactac ctcctggaa acgaacaac gtggcagct 
      agtcccgac ctcttcgcc tcctacatc cccatgcac 
      gggttcgtc cccgggatg aaaatcgag gcggctgac 
      ttgatggac cctagatta gtttgtgtg gcgacgatt 
      gccaaagtt gcagggagg ttgctcaag gtgcacttt 
      gacggttgg gaggaggag tatgaccag tggttggat 
      tgcgaaagt cccgatatt tatcccgtg ggatggtgt 
      caaagtgtg ggacacaag ctggaagga ccgccggtt 
      atccctcca aaacccaca actcccgtg gcgaagctc 
      gccaaaggg atgaagaaa agagggcgt aaaaagaaa 
      atcaaagaa gtgtcgccc aaagcggcc acgaaagtt 
      attaaagcc gaagtggaa ccggaaatt gagagggag 
      gaaatggaa gttgctgat gacaatcaa ggaccggaa 
      gcgggacaa gaacctgca ggacctgac caaagtctg 
      ccggtggcg accgacgcc aatccgcct ccggagcgc 
      aaagcgaca tcatatatt aatgcaaca aacccaaca 
      agtacaaaa ttaatacct cgtttagtg gatagtaca 
      ggaacaagt agcgaaact ggcgaactg tgtccagac 
      gaatggaat atttacgac gttgcgcaa tttttaagg 
      gttaatgat tgtgcgaat tattgcgac tctttcagt 
      aaacagaaa gtggacggt aaaacgttg atgaatctc 
      agtaaggag gatatttta gaatacaca gggggtaaa 
      gtgggccca tctctaaaa atattcgat ctcatacaa 
      cagttaaaa ataaaagtg aatccagcg caaattcgc 
      catatgaaa gcaaatatc aaaaagttt ttatag
    mRNA sequence
    • >TC007651 RA
      gcagcaggt gttctcaaa aaattcaca aatgatgcg 
      ttcggtgga caggctgag tgtaaattt taccacatt 
      ttataaatg aaaattatc acagttcgg ctgtcgcat 
      tagttagga ataaatttg gccatcgga aatgaacgt 
      gtacacaat gcccggagt ggccgacat gggcatggt 
      ctggatggg tgctgggca gcccaaccc cgacatgtc 
      catggaact catgtccgg ggacaactc ggacatgct 
      gctggagca cgagaaccc atatttcac cccatccat 
      acacagcat gtccaacca gtccatgga cgatatcca 
      cactatcca cagctcgca aatggacgc tatggaaaa 
      cctagatat gatgcaata tatcgacat gaagcacga 
      aagtaacga ctcaagtta tgccgaaga tgatagtca 
      gaaccctgt atatatgat aaactctgc cacgcaaac 
      attgccctg ctctacgcg gaaaatcaa accaatcaa 
      acaccctgg gttggtttt gaaaacacc aatagccta 
      ccaaagcaa tacggaccc ctcagtcat accaattca 
      aaaagatgg aattgctgt ttgcgagaa gtgcggcgc 
      catcggcgt caagcacgc cttttacac ccgagagcg 
      ccgcttctg cagcatggc ttgcgcccg cggctacag 
      cggcctgat ccccgagcc cttgcccct gtcccagca 
      gcaaacccc cgaacacca ccatttcgc caaacaccg 
      cttcaccat gaaaaccga agacgactt tgccaacac 
      agtcgtcga ccaggaatt cccccagct gcccccacc 
      gccagtgct gccccccat cgacgaaac agtcccgct 
      aatccgtcg caagccctc cgaactggc gaactcgca 
      cgactggga ttcgcaatt aaacgacca gtatttcgt 
      ggcagcccc tgtcacgtg tttcaaaca cgcgcccat 
      gtccgacat ttgggagaa tatcatggt cgggatgaa 
      agtcgaagt ggaaaatac cgactgtga taacgtctc 
      agaggcgtt tcccgactc gttttgggt ggctactgt 
      tttaaaaat cgtggggta taaggcgtt gctgcggta 
      cgaggggtt cgggtcgaa tgattccaa agatttttg 
      ggtctcgtt gtgttcgaa tcaggtgca tccggtcgg 
      gtggtgcgc cacgagggg gaagcccct gattccgcc 
      gaaaacgat cgaggacaa gtataacga ttggaagga 
      ttttttgag caagaggtt gacgggggc acgcacgtt 
      accgtcgaa ttttagcaa taaagcgag tgatagtct 
      taagtctag atttcaatg cggccttaa tttagaagt 
      tgtcgacaa aaatagaat ttcgcaagt taaggttgc 
      cattattca taaaatcgt ggggaagag gctgaatgt 
      gaagtattt taatatgcc gtcggatga tgcggggtt 
      ttggtgtca cgaggactc gccgcttct tcatcccgt 
      tggctgggc aaagaaggt cggccacca cctagtggc 
      ccctgtcaa ctacctgga gcgcgtcaa ccactgcat 
      ttacgacga cgacgacgc cactgacga gcttttcac 
      ccctttcca agtcggttc aaaagaacc cactgccga 
      cggcggctt ctgcatcgg aatgaaact cgaagcaat 
      cgatcctct caacctgtc ctcaatatg cgtcgccac 
      cgtaatgaa cgtcctacg ccacggcta catcatgat 
      ccggataga cacctacga atcggatat gaccggagc 
      cgattggtt ctgctacca cgtcaaatc cccgtgcat 
      tttccccgt cggtttttg cgaaaaata cgacattcc 
      cctaacccc tccaaaagg ctacgacca ggacacctt 
      cacctggcg gaactacct cctggaaac gaacaacgt 
      ggcagctag tcccgacct cttcgcctc ctacatccc 
      catgcacgg gttcgtccc cgggatgaa aatcgaggc 
      ggctgactt gatggaccc tagattagt ttgtgtggc 
      gacgattgc caaagttgc agggaggtt gctcaaggt 
      gcactttga cggttggga ggaggagta tgaccagtg 
      gttggattg cgaaagtcc cgatattta tcccgtggg 
      atggtgtca aagtgtggg acacaagct ggaaggacc 
      gccggttat ccctccaaa acccacaac tcccgtggc 
      gaagctcgc caaagggat gaagaaaag agggcgtaa 
      aaagaaaat caaagaagt gtcgcccaa agcggccac 
      gaaagttat taaagccga agtggaacc ggaaattga 
      gagggagga aatggaagt tgctgatga caatcaagg 
      accggaagc gggacaaga acctgcagg acctgacca 
      aagtctgcc ggtggcgac cgacgccaa tccgcctcc 
      ggagcgcaa agcgacatc atatattaa tgcaacaaa 
      cccaacaag tacaaaatt aatacctcg tttagtgga 
      tagtacagg aacaagtag cgaaactgg cgaactgtg 
      tccagacga atggaatat ttacgacgt tgcgcaatt 
      tttaagggt taatgattg tgcgaatta ttgcgactc 
      tttcagtaa acagaaagt ggacggtaa aacgttgat 
      gaatctcag taaggagga tattttaga atacacagg 
      gggtaaagt gggcccatc tctaaaaat attcgatct 
      catacaaca gttaaaaat aaaagtgaa tccagcgca 
      aattcgcca tatgaaagc aaatatcaa aaagttttt 
      atagtgcct aaatctgtt aatgtgtat ataaaaata 
      ggtcttaaa atattttat atattatta agcttggta 
      tttatcaac tagtttaat tttgtgtgt gtcgcccgg 
      aatttattt ctgtttttc ttcactttg tattcccta 
      tgttaagtt ctacataat atttattct atggtgata 
      gtgacgatt gaaaactta ctttttggt aatttgaac 
      attcaatgt ctcaaggaa attgtacag ttatgtcgt 
      ggaacaatt gtaattgta agcatgaat ccttagttt 
      tttaacaaa tcggttttc tgtttatta acttgtgct 
      tttacacag tttattttg tacgagtac ttatttttt 
      gacttctta gagatatga aaaataagt acggaaatt 
      agatcaaag attctgcat ttgtcagac agccacatg 
      atatttcat taggtacat tttgaaata caaatggtc 
      aaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY