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Gene information
General information
TC030745 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1418356..1420919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030745
    iBeetle screen iB_09847

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030745 RA
      MLTLLSEIH FWSTQEDVI VKHPDFGSF KYYSKKDPS 
      DSLCYQSKK KYCEQRGVI WHLPNKRPQ KLRDRLKEL 
      EEYLLHNKI VCFSWSDNH LQLLLSTGV VVTLTIDIT 
      TGDLTSLTF DKSLATKLQ VNILCDGVI LGPQIICIC 
      NDGHVLGFG GSWKDGWIV EGGPRRHLN FHNDWLAVW 
      GKCEAEHPQ PWSPLGKDY QRANLHLYW VGVRGPELL 
      AYKKTDGDP IEVIVSRTI RRTLFVIEQ KVTLRGAVS 
      VELSNFELV GANLKRVSV ISVPLQTQV SCCALNEQE 
      TRLLIGCID GSLALLDRV RGSTRTVKG LFIPKFARW 
      NSLGAVVTV LNERGQLQY FDTALNGVK SQIMGEDCT 
      PTALIDLSG YFNSQFTIS SVSWGPKDL VVVLEQGPM 
      LLITHIDRS LSFKSLSQR YIKMGKIDK AIRLLLSWE 
      FNEESFHVL QKIVAYLLS QPMTDDTAK YLQDALGCF 
      HSPPVPLNP ETRSRFFTQ IFSLTRRFF HQLVRAQMF 
      ETAFLLAVD LGHHDLFMD LHYIAVQIG ETEMAAAAR 
      AQASALLSR CSSEASNCT RSSCSQCSG SSDENPDNY 
      LTTNFNNPQ PKTYPRATF VKAPQVPAN FKAPPLPNL 
      GIPRNVFKP PLPSTGAVK KVPPKVKFS DTVTAFIVP 
      EIKRPARPP PPPHFTDPQ KELADSLPL CHPNEDYLK 
      DFNPVRRRE SEEAQPKIK VVHFGVV
    CDS sequence
    • >TC030745 RA
      atgttgacc cttttgagc gaaatccat ttctggagc 
      acccaagaa gacgtcatc gtaaaacat ccagatttt 
      ggatcattc aaatactac tctaagaaa gatcccagc 
      gactccctc tgctaccaa agcaaaaaa aagtactgc 
      gaacaacga ggtgtcatc tggcacttg cccaacaaa 
      cgcccccaa aaactccgc gaccgtctc aaagaactc 
      gaggagtat ctcctccac aacaaaatc gtctgtttc 
      tcttggtcc gacaaccac ctccaactt ctgctctca 
      actggggtc gtcgtcacc ctcaccatc gacatcacc 
      acaggcgac ctcacaagc ctcactttc gacaaatcc 
      ctcgccacc aaactccaa gtcaacata ttatgcgac 
      ggtgttatc ctcggcccc caaatcatc tgcatttgt 
      aatgacggc cacgtgctc ggcttcggg ggctcctgg 
      aaagacggc tggatcgtc gaagggggc cctcgacgc 
      cacctgaac ttccacaac gactggctt gccgtttgg 
      ggcaaatgc gaggccgaa cacccgcag ccttggagc 
      ccccttggc aaggactac caacgggct aatctacac 
      ctgtactgg gtgggcgtc cgggggcct gaactctta 
      gcgtacaaa aaaaccgac ggggatccc attgaagtg 
      atcgtcagt cggacaata cgccgaact ttgttcgta 
      atcgaacag aaggtgact ctcaggggg gctgtctca 
      gtcgagttg agcaatttt gagctggtt ggggccaat 
      ttgaaacgg gtttcggtc atttcggta ccacttcag 
      acacaagtc agttgctgt gccttaaat gagcaggaa 
      actaggctc ctgattggt tgtattgat gggtctttg 
      gcgttattg gaccgtgtc agggggtca actcggacg 
      gttaaaggg ttgtttata ccaaagttc gcacgttgg 
      aattctctg ggagctgtt gtgaccgtt ttgaatgaa 
      agggggcaa ttgcagtat tttgacacg gctttgaac 
      ggcgttaag tcacaaata atgggggag gattgcacc 
      ccaacagct ttaattgac ttatccggt tatttcaat 
      tcgcaattt acgatcagt tcggtgagt tggggccca 
      aaagactta gtcgtggtt ttggagcaa ggccccatg 
      ttactcatc actcacatt gaccggtct ttatcgttc 
      aaatctctc tcacaacgt tacataaaa atgggtaaa 
      attgataaa gccattcgg cttctccta agctgggaa 
      ttcaacgag gaaagtttc cacgtgttg caaaaaatc 
      gtcgcttat ttactctcc cagccaatg accgacgac 
      accgccaag tatctccag gacgccctc gggtgcttc 
      cacagcccc ccggtgccc ctgaacccc gagacccgt 
      tcccgcttc ttcacccaa attttctcg ctcactcgt 
      cgctttttc caccagtta gtccgcgcc caaatgttc 
      gaaaccgcc ttcctcttg gctgtggac ctagggcac 
      catgacctc ttcatggac ttgcactac atcgcggtg 
      caaatcggc gagacggag atggcggct gccgcccgc 
      gcccaggcc tcggccctc ctgagtcgc tgctccagc 
      gaggcctcg aactgcacc aggtcctcg tgctcccaa 
      tgttcggga agtagtgac gaaaacccc gataattac 
      ctcacaact aacttcaat aacccccaa ccgaagacc 
      tacccgaga gccactttc gtgaaggcg ccgcaagtg 
      ccggcgaat tttaaagcg cccccgttg cccaatttg 
      gggattccc agaaatgtg tttaagccg ccgctgccc 
      tcaacgggg gcggttaag aaggtgccc ccgaaagtt 
      aagttttct gatacggtg acggcgttt atcgtgccg 
      gagattaag cggccagcg aggccgccg ccgccacca 
      catttcacc gatccgcag aaagagttg gctgatagt 
      ttgccgctt tgtcaccca aatgaggat tatttgaag 
      gattttaac cctgtgagg aggagggag agcgaggaa 
      gcgcaaccc aaaatcaaa gtggtgcat tttggggtt 
      gtttaa
    mRNA sequence
    • >TC030745 RA
      cctgatttg gggaattta cacatcggt attattatt 
      attgttcga attttttaa tttatgaat taatgaagt 
      gatgagact caaaaagaa gcatttatt ttgcaaaag 
      tgactattt aatcaaaaa tcggccgta aaatgttga 
      cccttttga gcgaaatcc atttctgga gcacccaag 
      aagacgtca tcgtaaaac atccagatt ttggatcat 
      tcaaatact actctaaga aagatccca gcgactccc 
      tctgctacc aaagcaaaa aaaagtact gcgaacaac 
      gaggtgtca tctggcact tgcccaaca aacgccccc 
      aaaaactcc gcgaccgtc tcaaagaac tcgaggagt 
      atctcctcc acaacaaaa tcgtctgtt tctcttggt 
      ccgacaacc acctccaac ttctgctct caactgggg 
      tcgtcgtca ccctcacca tcgacatca ccacaggcg 
      acctcacaa gcctcactt tcgacaaat ccctcgcca 
      ccaaactcc aagtcaaca tattatgcg acggtgtta 
      tcctcggcc cccaaatca tctgcattt gtaatgacg 
      gccacgtgc tcggcttcg ggggctcct ggaaagacg 
      gctggatcg tcgaagggg gccctcgac gccacctga 
      acttccaca acgactggc ttgccgttt ggggcaaat 
      gcgaggccg aacacccgc agccttgga gcccccttg 
      gcaaggact accaacggg ctaatctac acctgtact 
      gggtgggcg tccgggggc ctgaactct tagcgtaca 
      aaaaaaccg acggggatc ccattgaag tgatcgtca 
      gtcggacaa tacgccgaa ctttgttcg taatcgaac 
      agaaggtga ctctcaggg gggctgtct cagtcgagt 
      tgagcaatt ttgagctgg ttggggcca atttgaaac 
      gggtttcgg tcatttcgg taccacttc agacacaag 
      tcagttgct gtgccttaa atgagcagg aaactaggc 
      tcctgattg gttgtattg atgggtctt tggcgttat 
      tggaccgtg tcagggggt caactcgga cggttaaag 
      ggttgttta taccaaagt tcgcacgtt ggaattctc 
      tgggagctg ttgtgaccg ttttgaatg aaagggggc 
      aattgcagt attttgaca cggctttga acggcgtta 
      agtcacaaa taatggggg aggattgca ccccaacag 
      ctttaattg acttatccg gttatttca attcgcaat 
      ttacgatca gttcggtga gttggggcc caaaagact 
      tagtcgtgg ttttggagc aaggcccca tgttactca 
      tcactcaca ttgaccggt ctttatcgt tcaaatctc 
      tctcacaac gttacataa aaatgggta aaattgata 
      aagccattc ggcttctcc taagctggg aattcaacg 
      aggaaagtt tccacgtgt tgcaaaaaa tcgtcgctt 
      atttactct cccagccaa tgaccgacg acaccgcca 
      agtatctcc aggacgccc tcgggtgct tccacagcc 
      ccccggtgc ccctgaacc ccgagaccc gttcccgct 
      tcttcaccc aaattttct cgctcactc gtcgctttt 
      tccaccagt tagtccgcg cccaaatgt tcgaaaccg 
      ccttcctct tggctgtgg acctagggc accatgacc 
      tcttcatgg acttgcact acatcgcgg tgcaaatcg 
      gcgagacgg agatggcgg ctgccgccc gcgcccagg 
      cctcggccc tcctgagtc gctgctcca gcgaggcct 
      cgaactgca ccaggtcct cgtgctccc aatgttcgg 
      gaagtagtg acgaaaacc ccgataatt acctcacaa 
      ctaacttca ataaccccc aaccgaaga cctacccga 
      gagccactt tcgtgaagg cgccgcaag tgccggcga 
      attttaaag cgcccccgt tgcccaatt tggggattc 
      ccagaaatg tgtttaagc cgccgctgc cctcaacgg 
      gggcggtta agaaggtgc ccccgaaag ttaagtttt 
      ctgatacgg tgacggcgt ttatcgtgc cggagatta 
      agcggccag cgaggccgc cgccgccac cacatttca 
      ccgatccgc agaaagagt tggctgata gtttgccgc 
      tttgtcacc caaatgagg attatttga aggatttta 
      accctgtga ggaggaggg agagcgagg aagcgcaac 
      ccaaaatca aagtggtgc attttgggg ttgtttaat 
      tcttttaga ataattgaa gacagtttc cgtccgtta 
      gaagtacaa ataaattac ccaaggagc cccagattg 
      tattaaaat atatttaac atgttttat ctgtggagg 
      aacgaaatt tgatatgta tatttaatt aatgagtag 
      attttttat tttttataa cttcgtatt ttcgcaaat 
      aaatatttt tattacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY