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Gene information
General information
TC007817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 964203..966842
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007817
    iBeetle screen iB_09845

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007817 RA
      MDPQPSKSD IEAVFHRLR ANPANKVCF DCNAKNPTW 
      ASVTYGVFI CIDCSAVHR SLGVHLTFV RSTQLDTNW 
      TWVQLRQMQ LGGNSNAIQ FFSQHNCMT TDAQKKYNS 
      RAAQLYKDK LHQAALNSL KSNTQLHIH PHVEERSEE 
      KEVDFFTEQ ENFAIPDAP QGHQHKIEE SQSLQKDNK 
      NPDEGPNVD FSAPAANPE PRKSTIATR KPPNKKSGL 
      GAKKGGLGA TKVKTNFAE IEREAEIAE ESRLRALEE 
      SAKAAALSL KEQEEREAA VRLAYKDLS NEQMKKEEQ 
      LRRQDPKKA EQVERLGMG VNARSGVSH SALSDMTEI 
      EQENVQPTP STLSSLGKL RISDSDSFF DEYSFSSGF 
      NINRANKMD MFGLESSRE RDKEKDNWV IVEDPVEKP 
      KPTYRTPEK VTKSFDLSS DAAQKKFGN AKAISSDQF 
      FNDREPDYE TKANLNRFQ GSSSISSAE FFGNGKEAT 
      PSSHMQAYD LDDVKESVR QGVTRIAGK FSYLANEVM 
      SSLQDRYGN
    CDS sequence
    • >TC007817 RA
      atggaccca caacctagt aaatcagat atcgaggct 
      gtttttcac cgattacga gccaatcct gccaataag 
      gtttgtttc gactgcaat gccaagaac ccgacttgg 
      gccagcgtg acatacggc gttttcatc tgcattgac 
      tgctccgca gttcacaga agtctcggt gttcatttg 
      acatttgtt aggtccacc cagcttgac accaactgg 
      acatgggtc cagctgaga caaatgcaa ctggggggc 
      aactccaac gccatccaa tttttctcg caacacaat 
      tgcatgaca acagatgct cagaaaaaa tataactct 
      cgagctgct cagctgtat aaagataaa ctgcaccaa 
      gcggctcta aactcgctt aaaagtaat acacagctt 
      catattcat ccacatgtt gaggagcgt agtgaagag 
      aaagaagtt gatttcttc acagaacag gaaaatttt 
      gccatccca gacgcgcct caaggacac caacacaaa 
      attgaagaa tcacagtca ctacaaaag gacaataaa 
      aatccagac gagggcccc aatgttgat tttagtgct 
      ccggcagcg aatccggag ccacgaaaa tcaaccatt 
      gccaccagg aagccccct aacaaaaaa tcagggttg 
      ggggcgaag aaaggcggc ttgggagcg acgaaagtg 
      aagacgaac tttgccgaa attgaacgt gaggccgaa 
      attgctgaa gaaagtcgt ctcagggct ttagaagaa 
      tcagcgaaa gctgcagct ttgtcttta aaggagcaa 
      gaagaacgt gaagctgcc gtccgattg gcttacaaa 
      gatctcagc aatgaacaa atgaagaaa gaagagcaa 
      ttacgaagg caagacccg aaaaaagcc gaacaggtt 
      gagagatta gggatggga gtgaacgct agaagtggg 
      gtcagccat tcggcttta agcgatatg acagagatt 
      gaacaagag aacgttcaa ccgactcct agtactttg 
      agttcgttg ggaaaatta cgaatttcc gacagcgat 
      agcttcttt gatgagtat tcgtttagt tccggtttt 
      aatataaac cgcgccaat aaaatggac atgtttggg 
      ttggaaagt agtcgggaa cgtgacaag gaaaaggat 
      aattgggtc attgttgag gatccggtc gaaaaaccg 
      aaaccgact taccgtacc ccagaaaaa gtaacgaaa 
      agtttcgat ttgagttca gacgccgcc cagaaaaaa 
      ttcggcaat gctaaagcc atctcttcc gatcagttt 
      ttcaacgat cgggagccc gattacgag acgaaggcg 
      aatctgaat cgatttcag ggttcttcc agtatttca 
      tcagccgag tttttcggc aatggcaaa gaagccact 
      ccaagttcg cacatgcaa gcgtatgat ttggacgac 
      gtcaaggag agcgtgaga caaggagtg acgcgaatc 
      gccggcaag ttcagttat ttagctaat gaagtcatg 
      tcttcgttg caagatcgc tacggtaac tag
    mRNA sequence
    • >TC007817 RA
      caacgtgtc aaatccact gacagtatc aatttgtca 
      tcgtattgt agataaaat ttaggtgaa atggaccca 
      caacctagt aaatcagat atcgaggct gtttttcac 
      cgattacga gccaatcct gccaataag gtttgtttc 
      gactgcaat gccaagaac ccgacttgg gccagcgtg 
      acatacggc gttttcatc tgcattgac tgctccgca 
      gttcacaga agtctcggt gttcatttg acatttgtt 
      aggtccacc cagcttgac accaactgg acatgggtc 
      cagctgaga caaatgcaa ctggggggc aactccaac 
      gccatccaa tttttctcg caacacaat tgcatgaca 
      acagatgct cagaaaaaa tataactct cgagctgct 
      cagctgtat aaagataaa ctgcaccaa gcggctcta 
      aactcgctt aaaagtaat acacagctt catattcat 
      ccacatgtt gaggagcgt agtgaagag aaagaagtt 
      gatttcttc acagaacag gaaaatttt gccatccca 
      gacgcgcct caaggacac caacacaaa attgaagaa 
      tcacagtca ctacaaaag gacaataaa aatccagac 
      gagggcccc aatgttgat tttagtgct ccggcagcg 
      aatccggag ccacgaaaa tcaaccatt gccaccagg 
      aagccccct aacaaaaaa tcagggttg ggggcgaag 
      aaaggcggc ttgggagcg acgaaagtg aagacgaac 
      tttgccgaa attgaacgt gaggccgaa attgctgaa 
      gaaagtcgt ctcagggct ttagaagaa tcagcgaaa 
      gctgcagct ttgtcttta aaggagcaa gaagaacgt 
      gaagctgcc gtccgattg gcttacaaa gatctcagc 
      aatgaacaa atgaagaaa gaagagcaa ttacgaagg 
      caagacccg aaaaaagcc gaacaggtt gagagatta 
      gggatggga gtgaacgct agaagtggg gtcagccat 
      tcggcttta agcgatatg acagagatt gaacaagag 
      aacgttcaa ccgactcct agtactttg agttcgttg 
      ggaaaatta cgaatttcc gacagcgat agcttcttt 
      gatgagtat tcgtttagt tccggtttt aatataaac 
      cgcgccaat aaaatggac atgtttggg ttggaaagt 
      agtcgggaa cgtgacaag gaaaaggat aattgggtc 
      attgttgag gatccggtc gaaaaaccg aaaccgact 
      taccgtacc ccagaaaaa gtaacgaaa agtttcgat 
      ttgagttca gacgccgcc cagaaaaaa ttcggcaat 
      gctaaagcc atctcttcc gatcagttt ttcaacgat 
      cgggagccc gattacgag acgaaggcg aatctgaat 
      cgatttcag ggttcttcc agtatttca tcagccgag 
      tttttcggc aatggcaaa gaagccact ccaagttcg 
      cacatgcaa gcgtatgat ttggacgac gtcaaggag 
      agcgtgaga caaggagtg acgcgaatc gccggcaag 
      ttcagttat ttagctaat gaagtcatg tcttcgttg 
      caagatcgc tacggtaac tagcagtta gtgaaaatt 
      gaaaaaaac taagcttta tcttggcac atttacaat 
      aaatcgttg aaatgatta tatatttta tccagttga 
      aaatgtgcc ttgatttta ttattaagg gggttaatg 
      ttgtaatta tgtaggcac ggataatag cactgtgac 
      atttttatc aaaatcacc taaacaaga tttaagagt 
      tagacagtg ccatttgtt ctactttgt taagtcttg 
      tttaaagtt ttatttttt ctctattat gtatcaaat 
      ttattttat tgtgacgat gcaattgtt tacctcgac 
      atttcagtt tatatattt ttttgaata aatatgcta 
      taaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY