Status
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Gene information
General information
TC032737
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007807
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 903713..907307
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032737
    iBeetle screen iB_09841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032737 RA
      MSQTDEGLE DLPEDEEEY EEEEEEEED HWSIYDAST 
      FISGPYQSE KCTINEGVL KFDFSFGYN CRKYFNLTA 
      PDPDTLIFA SGNFINFFD VLTKTISFR RSALGGGIA 
      HIKANPNPE FNHVAIAEN GKIPLIIIY EWPSMEINC 
      VLKGGSSRL YSHLDFSPD GLLLVSQGG EPDYLITIW 
      NWPKRRILL RTKSYVNDV HRVKFSPYC PGQLTSCGI 
      SHIKFWKMA RTFTGLKLK GELGRFGKT EFSDILGVL 
      PMPDEKVVS GCSWGNILV WDAGLIKLE VFRTLRRKC 
      HDGPIVQIS YNEGELMTV GMDGHVKVW WYEKIDQAD 
      PPDDDRVIQ IEPTYDFHT EGLMLMCMR KRYPDPKDT 
      SYFGQDGNG GLWIIDLNT EETPKESVQ LYKCHAGKV 
      VAIATSPNG PYLASLGED GRIYVYAYL EKRLIFNYQ 
      YPAKGTCMI WVPPKLAIY GDELLMGFD DGTLRLSIL 
      TINEDKKEV RVIQTTKPH DKPITQISI NPSNSVLVS 
      GGEDSTIFF FQIKFTIHV ELVPIGYIR VPNPVTCLT 
      WHCVNPYVI LVGCLRGEM VQVEVPKEP QPYTTVSYL 
      LDLEPKTNK FKTYKAQIR RDLKLKEIE ERKAEKVAK 
      KRVEMEKLK AENPGLDID EEVFLADSE TEEELEPLY 
      IPEIPNRVI WMQSTQDDV LWLSMGGYD AGYIYEYKI 
      EQKSDVPYF FKMVDEADD TEISSFVYN YNRKYLIFA 
      MENGEIRVN KIKDGDYHD LSDYWSLAM HDNQNGFVP 
      NMCFSYDEK FFFTCGHDG NVFSYTFQP DDDDYIPPE 
      DYPIVEERT SFPNSVPDE ANYDKLSLE QASLKKEQD 
      RIDRLAAKH REIYREKLR KLRERFFKL LKRNKKLLP 
      SQMIPVEDL EVDKRVTDY LDYKLEEEQ ALVKRKLAF 
      DVQKSELRM NKLRNYFID NLCFIPLIV SGLRSERLV 
      RMLRQRKVS PLNDQMLQI VDEKILEEE VKGRPPERA 
      PPAPKTIQP KKVKEQGLE YFLVNLDPK ILESQKHNK 
      LTRLLNKYR DRKLKWERR EEEVIN
    CDS sequence
    • >TC032737 RA
      atgtcgcaa actgacgaa ggtctcgaa gatcttcct 
      gaagatgag gaggaatac gaggaagaa gaggaggaa 
      gaggaggac cattggagc atttacgat gctagtacg 
      tttatttcc ggaccgtat caaagcgag aaatgtaca 
      atcaacgag ggtgtttta aaattcgat ttttcgttt 
      ggttacaac tgtcgcaaa tactttaat ctcactgca 
      cccgacccc gacactctg attttcgcc tcgggaaat 
      ttcatcaat tttttcgac gtactcacg aaaaccatt 
      tccttccgg cggagcgcc ttaggaggg ggaatcgca 
      catattaag gcgaatccc aacccggaa tttaaccac 
      gttgcgata gcagagaac ggcaaaatc cccttgatt 
      atcatctac gagtggcct tcgatggag atcaactgt 
      gtgctaaaa gggggctcc tcaaggctt tactcccat 
      ttagatttc agtcccgac ggactatta ctggtctcc 
      caaggcgga gaacccgac tatttgata acaatctgg 
      aactggccg aaacgtagg atcctactg aggaccaaa 
      tcctacgtc aacgacgtc catagggtg aaattctcg 
      ccctactgt cccggccaa ctgactagt tgtggcatc 
      tcccacatt aaattctgg aaaatggcc cggactttc 
      accggactt aaactcaaa ggtgaattg gggcggttt 
      ggcaagact gaattttcg gacattttg ggggtacta 
      ccaatgccg gacgaaaaa gtcgtgtct ggttgttcc 
      tggggcaat attttggtc tgggacgct ggattaatt 
      aaattggag gtgtttcgc accttaagg aggaagtgt 
      cacgatggt cccatcgtc caaatttcg tacaatgag 
      ggcgaattg atgactgtg ggaatggat gggcatgtc 
      aaagtctgg tggtacgaa aaaatcgac caagccgac 
      cctccagac gatgacaga gtcattcaa attgaaccg 
      acttatgat tttcacacc gaggggctt atgttgatg 
      tgtatgcga aaaaggtac cccgacccc aaagacacg 
      agttatttt ggtcaagac ggcaatggg ggtttatgg 
      attatagac ctgaatact gaagaaaca ccgaaagaa 
      tcggtccag ttgtacaaa tgtcacgct ggtaaagtc 
      gtggcaatt gccacaagt cccaatggg ccttatttg 
      gccagtttg ggcgaagat gggcgcatt tatgtctac 
      gcttatttg gagaaaagg ctcattttt aactatcag 
      tacccggcg aaaggtact tgcatgatc tgggttcca 
      ccaaagctt gccatctac ggtgacgaa ctattgatg 
      ggtttcgac gatggtacc ttgagattg tcaattttg 
      acaataaat gaagataaa aaggaagtc agggtcata 
      caaacgacc aaaccgcac gacaaaccc atcacccag 
      attagcatc aacccgtcg aatagtgtt ctagtgagc 
      ggtggcgaa gactcgact atatttttc ttccagatc 
      aaatttacc attcatgtc gagttggta cccattggg 
      tacattcgg gtaccaaac cctgtgaca tgtttgacc 
      tggcattgt gtcaatccc tacgtaatt ctcgttggc 
      tgtctccga ggcgaaatg gtgcaagtt gaggtaccg 
      aaagaacca caaccgtac accactgtc tcgtatttg 
      ctcgatttg gagccgaaa acgaacaaa ttcaagacg 
      tacaaagcg cagattcgg cgagatctc aaactgaag 
      gagatcgag gaacgtaag gcggagaaa gtcgccaag 
      aaaagggtg gaaatggag aaattgaaa gcggaaaat 
      ccgggttta gacatcgac gaggaagtt tttctcgcc 
      gattcggag accgaagaa gaactggaa cctttgtac 
      attcccgag attccgaat cgggttatt tggatgcaa 
      agcacccaa gacgatgtc ctttggttg tcgatgggt 
      ggctacgac gctggttat atttacgag tataaaatt 
      gagcaaaaa tcagatgtg ccctatttt tttaaaatg 
      gtggacgaa gctgacgat actgagatt agtagtttt 
      gtttacaat tacaatcgt aaatattta attttcgcc 
      atggaaaat ggggaaatt cgggtgaat aaaatcaaa 
      gatggggat tatcatgac ctttccgat tattggtcc 
      ttggccatg catgataat cagaacggt ttcgtcccg 
      aatatgtgt ttcagttac gacgaaaaa ttttttttc 
      acgtgcgga cacgatggg aatgttttt tcctacact 
      tttcaaccg gatgatgat gattatatt ccaccagag 
      gattatccg attgttgag gaacgaacc tcgtttccg 
      aatagtgta ccagacgaa gcaaattac gataaatta 
      agcctggaa caggccagt ttgaagaaa gaacaagac 
      cgaattgat agacttgcg gccaaacac cgggaaatc 
      tatcgggaa aaattgcgt aaacttcgt gagagattt 
      ttcaaactt ttgaaacgc aacaaaaag cttctccca 
      agtcagatg attccagtt gaagattta gaagttgat 
      aagcgtgta accgattat ctcgattac aaattggag 
      gaagaacag gctttagtt aagcggaag ttggcgttc 
      gatgtacaa aaatccgaa ctgcggatg aacaaacta 
      cggaattat ttcatcgat aatttgtgt tttattccg 
      ttgattgtg agcggcctc cggagcgaa agactggtc 
      cgaatgtta cgtcagaga aaagtttcg ccactcaat 
      gaccaaatg ttacaaatt gttgatgaa aaaatcctc 
      gaagaggag gtcaaaggc agacccccc gaacgtgca 
      ccccctgct cccaaaacc atccaaccg aaaaaagtc 
      aaagaacaa ggtcttgaa tatttcctc gtcaatctc 
      gatccaaaa atattggaa agtcaaaaa cataataaa 
      ctcacacgt cttctaaac aagtacaga gacaggaaa 
      ctcaaatgg gaacgacgc gaagaagag gtaattaac 
      tga
    mRNA sequence
    • >TC032737 RA
      aacgattga tcaaacttg aaagatgtc gcaaactga 
      cgaaggtct cgaagatct tcctgaaga tgaggagga 
      atacgagga agaagagga ggaagagga ggaccattg 
      gagcattta cgatgctag tacgtttat ttccggacc 
      gtatcaaag cgagaaatg tacaatcaa cgagggtgt 
      tttaaaatt cgatttttc gtttggtta caactgtcg 
      caaatactt taatctcac tgcacccga ccccgacac 
      tctgatttt cgcctcggg aaatttcat caatttttt 
      cgacgtact cacgaaaac catttcctt ccggcggag 
      cgccttagg agggggaat cgcacatat taaggcgaa 
      tcccaaccc ggaatttaa ccacgttgc gatagcaga 
      gaacggcaa aatcccctt gattatcat ctacgagtg 
      gccttcgat ggagatcaa ctgtgtgct aaaaggggg 
      ctcctcaag gctttactc ccatttaga tttcagtcc 
      cgacggact attactggt ctcccaagg cggagaacc 
      cgactattt gataacaat ctggaactg gccgaaacg 
      taggatcct actgaggac caaatccta cgtcaacga 
      cgtccatag ggtgaaatt ctcgcccta ctgtcccgg 
      ccaactgac tagttgtgg catctccca cattaaatt 
      ctggaaaat ggcccggac tttcaccgg acttaaact 
      caaaggtga attggggcg gtttggcaa gactgaatt 
      ttcggacat tttgggggt actaccaat gccggacga 
      aaaagtcgt gtctggttg ttcctgggg caatatttt 
      ggtctggga cgctggatt aattaaatt ggaggtgtt 
      tcgcacctt aaggaggaa gtgtcacga tggtcccat 
      cgtccaaat ttcgtacaa tgagggcga attgatgac 
      tgtgggaat ggatgggca tgtcaaagt ctggtggta 
      cgaaaaaat cgaccaagc cgaccctcc agacgatga 
      cagagtcat tcaaattga accgactta tgattttca 
      caccgaggg gcttatgtt gatgtgtat gcgaaaaag 
      gtaccccga ccccaaaga cacgagtta ttttggtca 
      agacggcaa tgggggttt atggattat agacctgaa 
      tactgaaga aacaccgaa agaatcggt ccagttgta 
      caaatgtca cgctggtaa agtcgtggc aattgccac 
      aagtcccaa tgggcctta tttggccag tttgggcga 
      agatgggcg catttatgt ctacgctta tttggagaa 
      aaggctcat ttttaacta tcagtaccc ggcgaaagg 
      tacttgcat gatctgggt tccaccaaa gcttgccat 
      ctacggtga cgaactatt gatgggttt cgacgatgg 
      taccttgag attgtcaat tttgacaat aaatgaaga 
      taaaaagga agtcagggt catacaaac gaccaaacc 
      gcacgacaa acccatcac ccagattag catcaaccc 
      gtcgaatag tgttctagt gagcggtgg cgaagactc 
      gactatatt tttcttcca gatcaaatt taccattca 
      tgtcgagtt ggtacccat tgggtacat tcgggtacc 
      aaaccctgt gacatgttt gacctggca ttgtgtcaa 
      tccctacgt aattctcgt tggctgtct ccgaggcga 
      aatggtgca agttgaggt accgaaaga accacaacc 
      gtacaccac tgtctcgta tttgctcga tttggagcc 
      gaaaacgaa caaattcaa gacgtacaa agcgcagat 
      tcggcgaga tctcaaact gaaggagat cgaggaacg 
      taaggcgga gaaagtcgc caagaaaag ggtggaaat 
      ggagaaatt gaaagcgga aaatccggg tttagacat 
      cgacgagga agtttttct cgccgattc ggagaccga 
      agaagaact ggaaccttt gtacattcc cgagattcc 
      gaatcgggt tatttggat gcaaagcac ccaagacga 
      tgtcctttg gttgtcgat gggtggcta cgacgctgg 
      ttatattta cgagtataa aattgagca aaaatcaga 
      tgtgcccta tttttttaa aatggtgga cgaagctga 
      cgatactga gattagtag ttttgttta caattacaa 
      tcgtaaata tttaatttt cgccatgga aaatgggga 
      aattcgggt gaataaaat caaagatgg ggattatca 
      tgacctttc cgattattg gtccttggc catgcatga 
      taatcagaa cggtttcgt cccgaatat gtgtttcag 
      ttacgacga aaaattttt tttcacgtg cggacacga 
      tgggaatgt tttttccta cacttttca accggatga 
      tgatgatta tattccacc agaggatta tccgattgt 
      tgaggaacg aacctcgtt tccgaatag tgtaccaga 
      cgaagcaaa ttacgataa attaagcct ggaacaggc 
      cagtttgaa gaaagaaca agaccgaat tgatagact 
      tgcggccaa acaccggga aatctatcg ggaaaaatt 
      gcgtaaact tcgtgagag atttttcaa acttttgaa 
      acgcaacaa aaagcttct cccaagtca gatgattcc 
      agttgaaga tttagaagt tgataagcg tgtaaccga 
      ttatctcga ttacaaatt ggaggaaga acaggcttt 
      agttaagcg gaagttggc gttcgatgt acaaaaatc 
      cgaactgcg gatgaacaa actacggaa ttatttcat 
      cgataattt gtgttttat tccgttgat tgtgagcgg 
      cctccggag cgaaagact ggtccgaat gttacgtca 
      gagaaaagt ttcgccact caatgacca aatgttaca 
      aattgttga tgaaaaaat cctcgaaga ggaggtcaa 
      aggcagacc ccccgaacg tgcaccccc tgctcccaa 
      aaccatcca accgaaaaa agtcaaaga acaaggtct 
      tgaatattt cctcgtcaa tctcgatcc aaaaatatt 
      ggaaagtca aaaacataa taaactcac acgtcttct 
      aaacaagta cagagacag gaaactcaa atgggaacg 
      acgcgaaga agaggtaat taactgaga gtttgtctt 
      gccttacaa ggcagggaa aagacacgt ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY