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Gene information
General information
TC032729 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 657529..671271
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032729
    iBeetle screen iB_09839

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032729 RA
      MSATPGGRT GAAATPSNA VSLKSIEVP QALQNGEKF 
      IKWDEDSGV GTPVTLRVD KYGFYLHWV DQNHEMDLL 
      DIATIRDTR TGRYAKIPK DPKLRQIVT MGSQDTLEE 
      KTVTVCCAN DFVNMNFIN FCCTRKEIA RLWTDALMS 
      LAYNLNQIN GTANLYLLK AYTKLILVT DKTGKIPIK 
      NIIKMFAQS RDDKRRIET VLQSTGLPY GKNDTLSPE 
      KFQFEDFFN FYKNLTQRS EIEKVFNDI VGSKKHMTA 
      AQFVDFLNK TQRDPRLNE ILHPYANTA RAKDIIAQY 
      EPNKYNAQK GQLSFDGFL RYLMSEDNN IIAPSKYEL 
      CDEMDHPLA HYFINSSHN TYLTGHQLT GKSSTEMYR 
      QCLLAGCRC VELDFWNGR EEPIIVHGY TFVPEISAR 
      EVLEAIAES AFKTSDFPV VLSFENHCN PRQQAKIAN 
      YCREIFGEM LLEAPLEAY KLEPGQPLP PPSVLKRKI 
      IIKNKKKHH HHHHHHHHH KKSTSAQPE APPSEPTGN 
      GDVPHHAPP LQANCRQGS KDSTGTEDE ESSSDEDGE 
      TLETDNSTE PTNEGSDHK AATAGETEA GAEISALVN 
      YVQPVHFTS FENAKKKNR SYEMSSFDE KQATSLLKE 
      YPVQFVNYN KHQLSRVYP AGTRFDSSN FMPQVFWNA 
      GCQLVALNF QTLDLAMQL NLGIFEYNF RCGYLLKPE 
      FMRRTDRRF YPFAESTVD GIIAGTVKI TVISGQFLT 
      DKRVGTYVE VEMYGLPAD TVRKRFRTK TVPNNGINP 
      VYDEEPFVF KKVVLPDLA SIRIAAYEE SGRFIGHRV 
      LPVVGLCPG YRHVNLRTE IGQPLPLAT LFLHIVVKD 
      YVPDGLSDF AEALANPIK YQSDLEKRA QQLAVLTDD 
      MEPAEGTED QKKNSLSAK EQATNGSPI QRPLIATGA 
      ASVDATSDN EGPVIATSV DTKNPQHDS NSLPKNIEV 
      KEEKFEELI AEPLDKILD NKLVKEKKL ELDKKLENL 
      RKKHEKKKM TLQSQKSGD FSEKRSRLI NMKLVKRLS 
      SKNIVSGVF STESPSVEP LTCVADGPE TSSGDPDKP 
      SLPRSHSER LLSVNRDFV CQEKELREK YHEVIYNTA 
      EKVMKISQN NQLKTLKDQ FERETTDLM RKLQADHRE 
      EVKALAKKH RNREQFTRV KREAESAVV GRGVSEREK 
      LGQIFESRR EELQRQHEA VKDALAECR NKAKVALLK 
      EFETRMARV ESESGGVL
    CDS sequence
    • >TC032729 RA
      atgtcggcg actccggga ggcaggacc ggagcggcg 
      gccacccct tccaacgct gtctccctc aaatctatc 
      gaggtgccc caggccctg cagaacggt gaaaaattc 
      atcaaatgg gatgaggac tctggggtg ggaaccccc 
      gtcaccctg cgcgtggac aaatacggt ttctacctc 
      cactgggtg gaccaaaac cacgaaatg gaccttctg 
      gacatagcc accatcaga gatacgcgg acaggtcgc 
      tatgcaaaa atcccaaag gaccccaaa ttaagacag 
      atcgtgaca atgggctct caagacacg ctcgaagag 
      aagacagtg actgtttgt tgtgccaat gactttgtg 
      aatatgaat tttataaat ttctgttgt acgaggaaa 
      gaaattgca agattatgg acggacgca ttgatgagt 
      cttgcgtac aatcttaac caaatcaat ggcacggct 
      aatttgtac cttttgaag gcttacacg aaattgatt 
      ttggtcacc gacaagacg gggaaaatc cctattaaa 
      aacataata aaaatgttt gctcaaagc agagacgac 
      aagagacgg attgagacc gttttgcaa tcaacgggg 
      ctcccctat ggcaagaat gacacgcta agtcccgaa 
      aaattccaa tttgaagac ttttttaat ttttacaaa 
      aacttgacc caacgcagc gagatagag aaggttttt 
      aacgacatt gttggttca aaaaagcac atgacggcc 
      gcacaattc gtcgatttt ttgaacaaa acgcaacga 
      gatccgcgc ctgaacgaa attctacat ccttacgca 
      aacacagca cgcgccaaa gacatcatc gcacaatac 
      gagcccaac aaatacaat gcacaaaaa ggccagtta 
      tccttcgac ggttttctc cgctacctc atgtcggaa 
      gataacaat ataatagca ccgagtaaa tacgaactt 
      tgcgacgaa atggatcat cccctcgcc cactatttc 
      attaattcg tcgcacaat acttattta actggacac 
      caactaacg ggcaaatcc agcacagag atgtacaga 
      cagtgttta ttggctgga tgtcgttgc gtcgagttg 
      gatttttgg aatgggaga gaggaaccg atcattgtg 
      cacggttat acgtttgtt cctgaaatt tcagcaagg 
      gaagtgctt gaagcgatt gctgaaagt gcattcaaa 
      acgtccgat tttccggtg gttttgtcg tttgaaaat 
      cactgtaat ccgagacaa caggcgaaa atcgccaat 
      tattgtcgc gagattttc ggagaaatg ctgctagag 
      gcacctcta gaggcgtac aagttggag ccaggccag 
      cctttgcct ccaccatca gtcctgaag cgcaagata 
      ataataaag aataaaaaa aaacatcac caccaccac 
      caccatcat caccaccat aaaaaaagc acatcggcc 
      caaccggaa gcaccacca tcggaaccc accggaaac 
      ggtgacgtt ccacaccac gcccctcca ttgcaggcc 
      aattgcaga caaggttct aaagactca actgggaca 
      gaagatgaa gaatcgtca agtgacgaa gacggcgaa 
      acactcgaa acggacaat tcgacggaa ccgacgaat 
      gaaggaagc gatcacaaa gcggcaaca gctggagag 
      accgaggca ggagccgag atatcggct ctagtgaat 
      tacgtacaa ccggtgcat ttcaccagc ttcgaaaat 
      gcgaaaaaa aaaaaccgc agttatgaa atgtcgtca 
      tttgatgaa aagcaggca acttcactt ttgaaagaa 
      tatcccgtt cagttcgtc aactacaac aaacatcaa 
      ctgagccgc gtttacccg gctggcact cgtttcgac 
      tcatcaaac ttcatgcct caggttttt tggaatgcc 
      ggctgtcag ctcgttgct ttaaatttc caaacactt 
      gatttagcc atgcaatta aatttgggc atttttgag 
      tataatttt cgttgtggt tatttgctc aagcctgaa 
      tttatgcga cgaactgat cggcggttt tatcctttc 
      gctgaatca acagtcgat ggtataatc gcaggaacg 
      gtcaaaata actgtgata tctggacaa tttttgact 
      gataagaga gtagggact tatgtcgaa gtggaaatg 
      tatggccta ccagccgac acagtacgg aaacgcttc 
      cggacgaaa actgtgccc aataatgga attaatcca 
      gtctatgat gaggaacct ttcgttttt aaaaaagtc 
      gttctgcct gatttggct tcgattcga atcgcggcg 
      tatgaagaa tctgggcgt tttattgga catcgagtt 
      cttccagtt gttggactg tgtcctggt tatcgacac 
      gtaaattta cgaactgaa atcggacaa ccgttacct 
      ctggccacg ttatttctt catattgtg gtcaaggat 
      tatgtaccg gacggcctt tctgacttt gcagaggct 
      ttagccaac ccgatcaag tatcaaagt gatttggaa 
      aaacgcgca caacagtta gctgttctc actgatgac 
      atggaaccg gccgaaggg accgaagac cagaaaaaa 
      aatagcttg agtgccaaa gaacaagcc accaacgga 
      agtccaatc caacggccc ttgatagca acaggggcg 
      gcttcagtg gacgccacc tccgataac gaaggcccc 
      gtaatagcg accagtgtt gacacaaaa aatccacaa 
      cacgattcc aactcctta ccgaaaaat attgaagtc 
      aaagaagaa aagtttgaa gaactgata gccgaacca 
      ttggacaag attcttgac aataaatta gttaaggag 
      aaaaaattg gaattggac aagaaattg gagaattta 
      aggaaaaaa cacgagaag aagaaaatg acgttgcag 
      tcgcagaaa tcgggcgat ttttccgag aagaggtca 
      cgtttgatt aacatgaag ctggtcaag cgattatcc 
      agtaaaaat atcgtgagc ggcgtgttc tcgacggag 
      tcgccgagc gtggagccc ctcacgtgc gtggcggac 
      ggcccggag accagcagc ggcgacccc gacaagccg 
      tcgctgccc cgcagccac tcggagcgt ctcctgtcc 
      gttaaccgc gactttgtg tgtcaggaa aaggagctg 
      cgcgaaaaa taccacgaa gtgatttac aacacggcc 
      gaaaaagtg atgaaaatc tcacaaaac aaccaactg 
      aagacactc aaagaccag ttcgagcgc gaaaccacc 
      gacttgatg cgaaagtta caagccgac catcgagaa 
      gaagtcaaa gctttggcg aaaaagcac cgaaatcgt 
      gaacagttc acgagggtt aaaagggaa gcggagtct 
      gctgttgtg ggccggggg gtctcagag agggaaaag 
      ctggggcag atttttgag agtcggaga gaggagttg 
      caaaggcag catgaggcg gttaaggac gctttggcc 
      gagtgccgg aataaggcg aaggtggcg ctcctgaag 
      gagttcgag acgcggatg gctcgcgtg gagtccgaa 
      agcggtgga gttctgtga
    mRNA sequence
    • >TC032729 RA
      tgcgctgct gccgcagga cctggtaac aggcaaaca 
      gcagcagta gcaacagca gaagagctg tagattgtg 
      caagtcgat gtaaattgt catcaagca agcaaaaaa 
      aatccagtc gaaaaaatg gtgagccag cgaacgtat 
      cgttggctc ccttcgtgg agttaaccg gagtcagct 
      ggcgtgtgg ttttacggt cgtggtgct tgagaaaaa 
      ctagcaact tttcacaaa gaagagctg aaatgaacg 
      aacaagaac gggtctgaa agcgcgttt gtttgtgtg 
      ttggttgtt ggctggtgg tgagatggt gtgctgaag 
      gagctctcc agcgatgtc ggcgactcc gggaggcag 
      gaccggagc ggcggccac cccttccaa cgctgtctc 
      cctcaaatc tatcgaggt gccccaggc cctgcagaa 
      cggtgaaaa attcatcaa atgggatga ggactctgg 
      ggtgggaac ccccgtcac cctgcgcgt ggacaaata 
      cggtttcta cctccactg ggtggacca aaaccacga 
      aatggacct tctggacat agccaccat cagagatac 
      gcggacagg tcgctatgc aaaaatccc aaaggaccc 
      caaattaag acagatcgt gacaatggg ctctcaaga 
      cacgctcga agagaagac agtgactgt ttgttgtgc 
      caatgactt tgtgaatat gaattttat aaatttctg 
      ttgtacgag gaaagaaat tgcaagatt atggacgga 
      cgcattgat gagtcttgc gtacaatct taaccaaat 
      caatggcac ggctaattt gtacctttt gaaggctta 
      cacgaaatt gattttggt caccgacaa gacggggaa 
      aatccctat taaaaacat aataaaaat gtttgctca 
      aagcagaga cgacaagag acggattga gaccgtttt 
      gcaatcaac ggggctccc ctatggcaa gaatgacac 
      gctaagtcc cgaaaaatt ccaatttga agacttttt 
      taattttta caaaaactt gacccaacg cagcgagat 
      agagaaggt ttttaacga cattgttgg ttcaaaaaa 
      gcacatgac ggccgcaca attcgtcga ttttttgaa 
      caaaacgca acgagatcc gcgcctgaa cgaaattct 
      acatcctta cgcaaacac agcacgcgc caaagacat 
      catcgcaca atacgagcc caacaaata caatgcaca 
      aaaaggcca gttatcctt cgacggttt tctccgcta 
      cctcatgtc ggaagataa caatataat agcaccgag 
      taaatacga actttgcga cgaaatgga tcatcccct 
      cgcccacta tttcattaa ttcgtcgca caatactta 
      tttaactgg acaccaact aacgggcaa atccagcac 
      agagatgta cagacagtg tttattggc tggatgtcg 
      ttgcgtcga gttggattt ttggaatgg gagagagga 
      accgatcat tgtgcacgg ttatacgtt tgttcctga 
      aatttcagc aagggaagt gcttgaagc gattgctga 
      aagtgcatt caaaacgtc cgattttcc ggtggtttt 
      gtcgtttga aaatcactg taatccgag acaacaggc 
      gaaaatcgc caattattg tcgcgagat tttcggaga 
      aatgctgct agaggcacc tctagaggc gtacaagtt 
      ggagccagg ccagccttt gcctccacc atcagtcct 
      gaagcgcaa gataataat aaagaataa aaaaaaaca 
      tcaccacca ccaccacca tcatcacca ccataaaaa 
      aagcacatc ggcccaacc ggaagcacc accatcgga 
      acccaccgg aaacggtga cgttccaca ccacgcccc 
      tccattgca ggccaattg cagacaagg ttctaaaga 
      ctcaactgg gacagaaga tgaagaatc gtcaagtga 
      cgaagacgg cgaaacact cgaaacgga caattcgac 
      ggaaccgac gaatgaagg aagcgatca caaagcggc 
      aacagctgg agagaccga ggcaggagc cgagatatc 
      ggctctagt gaattacgt acaaccggt gcatttcac 
      cagcttcga aaatgcgaa aaaaaaaaa ccgcagtta 
      tgaaatgtc gtcatttga tgaaaagca ggcaacttc 
      acttttgaa agaatatcc cgttcagtt cgtcaacta 
      caacaaaca tcaactgag ccgcgttta cccggctgg 
      cactcgttt cgactcatc aaacttcat gcctcaggt 
      tttttggaa tgccggctg tcagctcgt tgctttaaa 
      tttccaaac acttgattt agccatgca attaaattt 
      gggcatttt tgagtataa ttttcgttg tggttattt 
      gctcaagcc tgaatttat gcgacgaac tgatcggcg 
      gttttatcc tttcgctga atcaacagt cgatggtat 
      aatcgcagg aacggtcaa aataactgt gatatctgg 
      acaattttt gactgataa gagagtagg gacttatgt 
      cgaagtgga aatgtatgg cctaccagc cgacacagt 
      acggaaacg cttccggac gaaaactgt gcccaataa 
      tggaattaa tccagtcta tgatgagga acctttcgt 
      ttttaaaaa agtcgttct gcctgattt ggcttcgat 
      tcgaatcgc ggcgtatga agaatctgg gcgttttat 
      tggacatcg agttcttcc agttgttgg actgtgtcc 
      tggttatcg acacgtaaa tttacgaac tgaaatcgg 
      acaaccgtt acctctggc cacgttatt tcttcatat 
      tgtggtcaa ggattatgt accggacgg cctttctga 
      ctttgcaga ggctttagc caacccgat caagtatca 
      aagtgattt ggaaaaacg cgcacaaca gttagctgt 
      tctcactga tgacatgga accggccga agggaccga 
      agaccagaa aaaaaatag cttgagtgc caaagaaca 
      agccaccaa cggaagtcc aatccaacg gcccttgat 
      agcaacagg ggcggcttc agtggacgc cacctccga 
      taacgaagg ccccgtaat agcgaccag tgttgacac 
      aaaaaatcc acaacacga ttccaactc cttaccgaa 
      aaatattga agtcaaaga agaaaagtt tgaagaact 
      gatagccga accattgga caagattct tgacaataa 
      attagttaa ggagaaaaa attggaatt ggacaagaa 
      attggagaa tttaaggaa aaaacacga gaagaagaa 
      aatgacgtt gcagtcgca gaaatcggg cgatttttc 
      cgagaagag gtcacgttt gattaacat gaagctggt 
      caagcgatt atccagtaa aaatatcgt gagcggcgt 
      gttctcgac ggagtcgcc gagcgtgga gcccctcac 
      gtgcgtggc ggacggccc ggagaccag cagcggcga 
      ccccgacaa gccgtcgct gccccgcag ccactcgga 
      gcgtctcct gtccgttaa ccgcgactt tgtgtgtca 
      ggaaaagga gctgcgcga aaaatacca cgaagtgat 
      ttacaacac ggccgaaaa agtgatgaa aatctcaca 
      aaacaacca actgaagac actcaaaga ccagttcga 
      gcgcgaaac caccgactt gatgcgaaa gttacaagc 
      cgaccatcg agaagaagt caaagcttt ggcgaaaaa 
      gcaccgaaa tcgtgaaca gttcacgag ggttaaaag 
      ggaagcgga gtctgctgt tgtgggccg gggggtctc 
      agagaggga aaagctggg gcagatttt tgagagtcg 
      gagagagga gttgcaaag gcagcatga ggcggttaa 
      ggacgcttt ggccgagtg ccggaataa ggcgaaggt 
      ggcgctcct gaaggagtt cgagacgcg gatggctcg 
      cgtggagtc cgaaagcgg tggagttct gtgactgtt 
      ttcctaaaa aacaatctt ttttccaag tctaactgt 
      gagttacct gatgcgaac cttctctag ttgtaaagt 
      gcaatattt gcggatatt gtcgtaatt tatttaacg 
      aatactgtc tttgatact ctcgacaat caaaaaaaa 
      aaaatgtta caaggtggc ccagatgtt tttaagtgc 
      tacagtcct ttggtcttg ttactataa tattgatgt 
      ttatcgttt attgtgata gtaggttag cgaaaatat 
      aatagagcg cgttttcaa gtgatacat tattgttaa 
      tttgcaacg taacgccag tgtatgatc ggtttattt 
      atccaaggc tgtggtaca caaacacgg ttatgagcc 
      agttggggg gaaaatatt tcgtttatt aagtggctg 
      tgaggagac atgacaaaa ttattcacg agttgacaa 
      taacgcaca caaaggaac taattatta tttggtgta 
      tttgtgtac ctcagcttt ttatgaaag catttttcg 
      ttggtgacg gatgtgcta gttttagga agtaagtag 
      aaaagtgcc accaactgt gaaatgtta tttcgtaat 
      agattacct gttatcaag tgtatttat ttatttttc 
      cattatttg tgctatgcg gtgggtcca aagcatccg 
      attttttac acaacaatt aattttttt ttggggatg 
      cttttggcc catttggga tcttccgag cgggtttag 
      tagatctag tcattttga tttcgtcgc aatacactc 
      agcattatc gattaaatc attctcaga cctcgttta 
      gtgatttta gggagcgct ttttccgga aacggcctg 
      aatttcgtt ttgttaaac atcaacagc ttaatattg 
      aatcttcga tgttgctga ctagtgttt gttgctact 
      aattgttaa tataactgg ttgttgaat atgtagtat 
      agaaaaaat tgttaggtg agacggcca ctcgtcctt 
      attccgctc taagctagt tgtctataa ataaaaact 
      ggaataagg gcgaccaac aactggcgg ttcaagtta 
      tgcgatagg tgtagtata aataaatta tttccctca 
      ttaggtatt gtactccat atgacatac tcgaaacgg 
      cctatttag tctaaatgt tttcgttga ttttttaac 
      catttgatt cgttcgaat tctattatc gcgttttgt 
      acgtatata ggtacttat ttttttgag tgctcaaga 
      gtattttga cgatcgatg agattttgt tgatttttg 
      tttctagtt gttttttga ttacattcc atttaagtt 
      tgttagtga gtgattatt gttgaatac atttacgaa 
      tttctgcat tttgtattt ttccaagtc gtgcctttt 
      aaccatgaa taattttac ttgtacact attcttact 
      catctgtgt attattaca tgtgtttac tatgaagtt 
      tgtgtcaga atagattta tgtgctcta ccacggctc 
      tcattcaaa caataaaaa ccgataaaa cac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY