| General information | |||
| TC032729 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 657529..671271 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032729 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09839 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
tropharium structure irregular - Number of animals showing the phenotype: 4
vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 4
previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032729 RA
MSATPGGRT GAAATPSNA VSLKSIEVP QALQNGEKF IKWDEDSGV GTPVTLRVD KYGFYLHWV DQNHEMDLL DIATIRDTR TGRYAKIPK DPKLRQIVT MGSQDTLEE KTVTVCCAN DFVNMNFIN FCCTRKEIA RLWTDALMS LAYNLNQIN GTANLYLLK AYTKLILVT DKTGKIPIK NIIKMFAQS RDDKRRIET VLQSTGLPY GKNDTLSPE KFQFEDFFN FYKNLTQRS EIEKVFNDI VGSKKHMTA AQFVDFLNK TQRDPRLNE ILHPYANTA RAKDIIAQY EPNKYNAQK GQLSFDGFL RYLMSEDNN IIAPSKYEL CDEMDHPLA HYFINSSHN TYLTGHQLT GKSSTEMYR QCLLAGCRC VELDFWNGR EEPIIVHGY TFVPEISAR EVLEAIAES AFKTSDFPV VLSFENHCN PRQQAKIAN YCREIFGEM LLEAPLEAY KLEPGQPLP PPSVLKRKI IIKNKKKHH HHHHHHHHH KKSTSAQPE APPSEPTGN GDVPHHAPP LQANCRQGS KDSTGTEDE ESSSDEDGE TLETDNSTE PTNEGSDHK AATAGETEA GAEISALVN YVQPVHFTS FENAKKKNR SYEMSSFDE KQATSLLKE YPVQFVNYN KHQLSRVYP AGTRFDSSN FMPQVFWNA GCQLVALNF QTLDLAMQL NLGIFEYNF RCGYLLKPE FMRRTDRRF YPFAESTVD GIIAGTVKI TVISGQFLT DKRVGTYVE VEMYGLPAD TVRKRFRTK TVPNNGINP VYDEEPFVF KKVVLPDLA SIRIAAYEE SGRFIGHRV LPVVGLCPG YRHVNLRTE IGQPLPLAT LFLHIVVKD YVPDGLSDF AEALANPIK YQSDLEKRA QQLAVLTDD MEPAEGTED QKKNSLSAK EQATNGSPI QRPLIATGA ASVDATSDN EGPVIATSV DTKNPQHDS NSLPKNIEV KEEKFEELI AEPLDKILD NKLVKEKKL ELDKKLENL RKKHEKKKM TLQSQKSGD FSEKRSRLI NMKLVKRLS SKNIVSGVF STESPSVEP LTCVADGPE TSSGDPDKP SLPRSHSER LLSVNRDFV CQEKELREK YHEVIYNTA EKVMKISQN NQLKTLKDQ FERETTDLM RKLQADHRE EVKALAKKH RNREQFTRV KREAESAVV GRGVSEREK LGQIFESRR EELQRQHEA VKDALAECR NKAKVALLK EFETRMARV ESESGGVL
- >TC032729 RA
atgtcggcg actccggga ggcaggacc ggagcggcg gccacccct tccaacgct gtctccctc aaatctatc gaggtgccc caggccctg cagaacggt gaaaaattc atcaaatgg gatgaggac tctggggtg ggaaccccc gtcaccctg cgcgtggac aaatacggt ttctacctc cactgggtg gaccaaaac cacgaaatg gaccttctg gacatagcc accatcaga gatacgcgg acaggtcgc tatgcaaaa atcccaaag gaccccaaa ttaagacag atcgtgaca atgggctct caagacacg ctcgaagag aagacagtg actgtttgt tgtgccaat gactttgtg aatatgaat tttataaat ttctgttgt acgaggaaa gaaattgca agattatgg acggacgca ttgatgagt cttgcgtac aatcttaac caaatcaat ggcacggct aatttgtac cttttgaag gcttacacg aaattgatt ttggtcacc gacaagacg gggaaaatc cctattaaa aacataata aaaatgttt gctcaaagc agagacgac aagagacgg attgagacc gttttgcaa tcaacgggg ctcccctat ggcaagaat gacacgcta agtcccgaa aaattccaa tttgaagac ttttttaat ttttacaaa aacttgacc caacgcagc gagatagag aaggttttt aacgacatt gttggttca aaaaagcac atgacggcc gcacaattc gtcgatttt ttgaacaaa acgcaacga gatccgcgc ctgaacgaa attctacat ccttacgca aacacagca cgcgccaaa gacatcatc gcacaatac gagcccaac aaatacaat gcacaaaaa ggccagtta tccttcgac ggttttctc cgctacctc atgtcggaa gataacaat ataatagca ccgagtaaa tacgaactt tgcgacgaa atggatcat cccctcgcc cactatttc attaattcg tcgcacaat acttattta actggacac caactaacg ggcaaatcc agcacagag atgtacaga cagtgttta ttggctgga tgtcgttgc gtcgagttg gatttttgg aatgggaga gaggaaccg atcattgtg cacggttat acgtttgtt cctgaaatt tcagcaagg gaagtgctt gaagcgatt gctgaaagt gcattcaaa acgtccgat tttccggtg gttttgtcg tttgaaaat cactgtaat ccgagacaa caggcgaaa atcgccaat tattgtcgc gagattttc ggagaaatg ctgctagag gcacctcta gaggcgtac aagttggag ccaggccag cctttgcct ccaccatca gtcctgaag cgcaagata ataataaag aataaaaaa aaacatcac caccaccac caccatcat caccaccat aaaaaaagc acatcggcc caaccggaa gcaccacca tcggaaccc accggaaac ggtgacgtt ccacaccac gcccctcca ttgcaggcc aattgcaga caaggttct aaagactca actgggaca gaagatgaa gaatcgtca agtgacgaa gacggcgaa acactcgaa acggacaat tcgacggaa ccgacgaat gaaggaagc gatcacaaa gcggcaaca gctggagag accgaggca ggagccgag atatcggct ctagtgaat tacgtacaa ccggtgcat ttcaccagc ttcgaaaat gcgaaaaaa aaaaaccgc agttatgaa atgtcgtca tttgatgaa aagcaggca acttcactt ttgaaagaa tatcccgtt cagttcgtc aactacaac aaacatcaa ctgagccgc gtttacccg gctggcact cgtttcgac tcatcaaac ttcatgcct caggttttt tggaatgcc ggctgtcag ctcgttgct ttaaatttc caaacactt gatttagcc atgcaatta aatttgggc atttttgag tataatttt cgttgtggt tatttgctc aagcctgaa tttatgcga cgaactgat cggcggttt tatcctttc gctgaatca acagtcgat ggtataatc gcaggaacg gtcaaaata actgtgata tctggacaa tttttgact gataagaga gtagggact tatgtcgaa gtggaaatg tatggccta ccagccgac acagtacgg aaacgcttc cggacgaaa actgtgccc aataatgga attaatcca gtctatgat gaggaacct ttcgttttt aaaaaagtc gttctgcct gatttggct tcgattcga atcgcggcg tatgaagaa tctgggcgt tttattgga catcgagtt cttccagtt gttggactg tgtcctggt tatcgacac gtaaattta cgaactgaa atcggacaa ccgttacct ctggccacg ttatttctt catattgtg gtcaaggat tatgtaccg gacggcctt tctgacttt gcagaggct ttagccaac ccgatcaag tatcaaagt gatttggaa aaacgcgca caacagtta gctgttctc actgatgac atggaaccg gccgaaggg accgaagac cagaaaaaa aatagcttg agtgccaaa gaacaagcc accaacgga agtccaatc caacggccc ttgatagca acaggggcg gcttcagtg gacgccacc tccgataac gaaggcccc gtaatagcg accagtgtt gacacaaaa aatccacaa cacgattcc aactcctta ccgaaaaat attgaagtc aaagaagaa aagtttgaa gaactgata gccgaacca ttggacaag attcttgac aataaatta gttaaggag aaaaaattg gaattggac aagaaattg gagaattta aggaaaaaa cacgagaag aagaaaatg acgttgcag tcgcagaaa tcgggcgat ttttccgag aagaggtca cgtttgatt aacatgaag ctggtcaag cgattatcc agtaaaaat atcgtgagc ggcgtgttc tcgacggag tcgccgagc gtggagccc ctcacgtgc gtggcggac ggcccggag accagcagc ggcgacccc gacaagccg tcgctgccc cgcagccac tcggagcgt ctcctgtcc gttaaccgc gactttgtg tgtcaggaa aaggagctg cgcgaaaaa taccacgaa gtgatttac aacacggcc gaaaaagtg atgaaaatc tcacaaaac aaccaactg aagacactc aaagaccag ttcgagcgc gaaaccacc gacttgatg cgaaagtta caagccgac catcgagaa gaagtcaaa gctttggcg aaaaagcac cgaaatcgt gaacagttc acgagggtt aaaagggaa gcggagtct gctgttgtg ggccggggg gtctcagag agggaaaag ctggggcag atttttgag agtcggaga gaggagttg caaaggcag catgaggcg gttaaggac gctttggcc gagtgccgg aataaggcg aaggtggcg ctcctgaag gagttcgag acgcggatg gctcgcgtg gagtccgaa agcggtgga gttctgtga
- >TC032729 RA
tgcgctgct gccgcagga cctggtaac aggcaaaca gcagcagta gcaacagca gaagagctg tagattgtg caagtcgat gtaaattgt catcaagca agcaaaaaa aatccagtc gaaaaaatg gtgagccag cgaacgtat cgttggctc ccttcgtgg agttaaccg gagtcagct ggcgtgtgg ttttacggt cgtggtgct tgagaaaaa ctagcaact tttcacaaa gaagagctg aaatgaacg aacaagaac gggtctgaa agcgcgttt gtttgtgtg ttggttgtt ggctggtgg tgagatggt gtgctgaag gagctctcc agcgatgtc ggcgactcc gggaggcag gaccggagc ggcggccac cccttccaa cgctgtctc cctcaaatc tatcgaggt gccccaggc cctgcagaa cggtgaaaa attcatcaa atgggatga ggactctgg ggtgggaac ccccgtcac cctgcgcgt ggacaaata cggtttcta cctccactg ggtggacca aaaccacga aatggacct tctggacat agccaccat cagagatac gcggacagg tcgctatgc aaaaatccc aaaggaccc caaattaag acagatcgt gacaatggg ctctcaaga cacgctcga agagaagac agtgactgt ttgttgtgc caatgactt tgtgaatat gaattttat aaatttctg ttgtacgag gaaagaaat tgcaagatt atggacgga cgcattgat gagtcttgc gtacaatct taaccaaat caatggcac ggctaattt gtacctttt gaaggctta cacgaaatt gattttggt caccgacaa gacggggaa aatccctat taaaaacat aataaaaat gtttgctca aagcagaga cgacaagag acggattga gaccgtttt gcaatcaac ggggctccc ctatggcaa gaatgacac gctaagtcc cgaaaaatt ccaatttga agacttttt taattttta caaaaactt gacccaacg cagcgagat agagaaggt ttttaacga cattgttgg ttcaaaaaa gcacatgac ggccgcaca attcgtcga ttttttgaa caaaacgca acgagatcc gcgcctgaa cgaaattct acatcctta cgcaaacac agcacgcgc caaagacat catcgcaca atacgagcc caacaaata caatgcaca aaaaggcca gttatcctt cgacggttt tctccgcta cctcatgtc ggaagataa caatataat agcaccgag taaatacga actttgcga cgaaatgga tcatcccct cgcccacta tttcattaa ttcgtcgca caatactta tttaactgg acaccaact aacgggcaa atccagcac agagatgta cagacagtg tttattggc tggatgtcg ttgcgtcga gttggattt ttggaatgg gagagagga accgatcat tgtgcacgg ttatacgtt tgttcctga aatttcagc aagggaagt gcttgaagc gattgctga aagtgcatt caaaacgtc cgattttcc ggtggtttt gtcgtttga aaatcactg taatccgag acaacaggc gaaaatcgc caattattg tcgcgagat tttcggaga aatgctgct agaggcacc tctagaggc gtacaagtt ggagccagg ccagccttt gcctccacc atcagtcct gaagcgcaa gataataat aaagaataa aaaaaaaca tcaccacca ccaccacca tcatcacca ccataaaaa aagcacatc ggcccaacc ggaagcacc accatcgga acccaccgg aaacggtga cgttccaca ccacgcccc tccattgca ggccaattg cagacaagg ttctaaaga ctcaactgg gacagaaga tgaagaatc gtcaagtga cgaagacgg cgaaacact cgaaacgga caattcgac ggaaccgac gaatgaagg aagcgatca caaagcggc aacagctgg agagaccga ggcaggagc cgagatatc ggctctagt gaattacgt acaaccggt gcatttcac cagcttcga aaatgcgaa aaaaaaaaa ccgcagtta tgaaatgtc gtcatttga tgaaaagca ggcaacttc acttttgaa agaatatcc cgttcagtt cgtcaacta caacaaaca tcaactgag ccgcgttta cccggctgg cactcgttt cgactcatc aaacttcat gcctcaggt tttttggaa tgccggctg tcagctcgt tgctttaaa tttccaaac acttgattt agccatgca attaaattt gggcatttt tgagtataa ttttcgttg tggttattt gctcaagcc tgaatttat gcgacgaac tgatcggcg gttttatcc tttcgctga atcaacagt cgatggtat aatcgcagg aacggtcaa aataactgt gatatctgg acaattttt gactgataa gagagtagg gacttatgt cgaagtgga aatgtatgg cctaccagc cgacacagt acggaaacg cttccggac gaaaactgt gcccaataa tggaattaa tccagtcta tgatgagga acctttcgt ttttaaaaa agtcgttct gcctgattt ggcttcgat tcgaatcgc ggcgtatga agaatctgg gcgttttat tggacatcg agttcttcc agttgttgg actgtgtcc tggttatcg acacgtaaa tttacgaac tgaaatcgg acaaccgtt acctctggc cacgttatt tcttcatat tgtggtcaa ggattatgt accggacgg cctttctga ctttgcaga ggctttagc caacccgat caagtatca aagtgattt ggaaaaacg cgcacaaca gttagctgt tctcactga tgacatgga accggccga agggaccga agaccagaa aaaaaatag cttgagtgc caaagaaca agccaccaa cggaagtcc aatccaacg gcccttgat agcaacagg ggcggcttc agtggacgc cacctccga taacgaagg ccccgtaat agcgaccag tgttgacac aaaaaatcc acaacacga ttccaactc cttaccgaa aaatattga agtcaaaga agaaaagtt tgaagaact gatagccga accattgga caagattct tgacaataa attagttaa ggagaaaaa attggaatt ggacaagaa attggagaa tttaaggaa aaaacacga gaagaagaa aatgacgtt gcagtcgca gaaatcggg cgatttttc cgagaagag gtcacgttt gattaacat gaagctggt caagcgatt atccagtaa aaatatcgt gagcggcgt gttctcgac ggagtcgcc gagcgtgga gcccctcac gtgcgtggc ggacggccc ggagaccag cagcggcga ccccgacaa gccgtcgct gccccgcag ccactcgga gcgtctcct gtccgttaa ccgcgactt tgtgtgtca ggaaaagga gctgcgcga aaaatacca cgaagtgat ttacaacac ggccgaaaa agtgatgaa aatctcaca aaacaacca actgaagac actcaaaga ccagttcga gcgcgaaac caccgactt gatgcgaaa gttacaagc cgaccatcg agaagaagt caaagcttt ggcgaaaaa gcaccgaaa tcgtgaaca gttcacgag ggttaaaag ggaagcgga gtctgctgt tgtgggccg gggggtctc agagaggga aaagctggg gcagatttt tgagagtcg gagagagga gttgcaaag gcagcatga ggcggttaa ggacgcttt ggccgagtg ccggaataa ggcgaaggt ggcgctcct gaaggagtt cgagacgcg gatggctcg cgtggagtc cgaaagcgg tggagttct gtgactgtt ttcctaaaa aacaatctt ttttccaag tctaactgt gagttacct gatgcgaac cttctctag ttgtaaagt gcaatattt gcggatatt gtcgtaatt tatttaacg aatactgtc tttgatact ctcgacaat caaaaaaaa aaaatgtta caaggtggc ccagatgtt tttaagtgc tacagtcct ttggtcttg ttactataa tattgatgt ttatcgttt attgtgata gtaggttag cgaaaatat aatagagcg cgttttcaa gtgatacat tattgttaa tttgcaacg taacgccag tgtatgatc ggtttattt atccaaggc tgtggtaca caaacacgg ttatgagcc agttggggg gaaaatatt tcgtttatt aagtggctg tgaggagac atgacaaaa ttattcacg agttgacaa taacgcaca caaaggaac taattatta tttggtgta tttgtgtac ctcagcttt ttatgaaag catttttcg ttggtgacg gatgtgcta gttttagga agtaagtag aaaagtgcc accaactgt gaaatgtta tttcgtaat agattacct gttatcaag tgtatttat ttatttttc cattatttg tgctatgcg gtgggtcca aagcatccg attttttac acaacaatt aattttttt ttggggatg cttttggcc catttggga tcttccgag cgggtttag tagatctag tcattttga tttcgtcgc aatacactc agcattatc gattaaatc attctcaga cctcgttta gtgatttta gggagcgct ttttccgga aacggcctg aatttcgtt ttgttaaac atcaacagc ttaatattg aatcttcga tgttgctga ctagtgttt gttgctact aattgttaa tataactgg ttgttgaat atgtagtat agaaaaaat tgttaggtg agacggcca ctcgtcctt attccgctc taagctagt tgtctataa ataaaaact ggaataagg gcgaccaac aactggcgg ttcaagtta tgcgatagg tgtagtata aataaatta tttccctca ttaggtatt gtactccat atgacatac tcgaaacgg cctatttag tctaaatgt tttcgttga ttttttaac catttgatt cgttcgaat tctattatc gcgttttgt acgtatata ggtacttat ttttttgag tgctcaaga gtattttga cgatcgatg agattttgt tgatttttg tttctagtt gttttttga ttacattcc atttaagtt tgttagtga gtgattatt gttgaatac atttacgaa tttctgcat tttgtattt ttccaagtc gtgcctttt aaccatgaa taattttac ttgtacact attcttact catctgtgt attattaca tgtgtttac tatgaagtt tgtgtcaga atagattta tgtgctcta ccacggctc tcattcaaa caataaaaa ccgataaaa cac
