Status
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Gene information
General information
TC007706 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 32536..34463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007706
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09836

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head appendages segmentation/division present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head potentially transformed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax potentially transformed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen potentially multiplied - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007706 RA
      MSSAHSNTL AQESSINDN FSGPESRFE CPICLAWLR 
DPVLTSCGH RFCRSCMDS WLERENPSC PIDNTKLEK 
GVDIFPDNF TRREISQQK TKCPNLVRG CLEELSPLD 
VETHLLTCQ YKPPELPEN EKLRCAFVE FGCEDKFED 
EPELQRHLE QYIHKHLNL LSQAYSKFT INSPSTSAI 
AQQANFWDP PSKNDTSVS SNHDNVNGL LKALYEKAV 
LLEQKTREQ DIIIANMSE QISSLNLSM SKMHLRYCN 
GCYLWYFND FKNKINAMR NDSRIMHYS PGFYTAANG 
YRLCIRLNL SPKDPNYIA ILIHVMKTE HDNALDWPF 
SGRLTITLV HPLQPFHNI KETMMTRPE LDAFKKPVQ 
DMNPKGFGY TEFALIEEL FSQGYVENN HLLIKVQAQ 
PV
    CDS sequence
    • >TC007706 RA
      atgtcttca gcccacagt aacaccctg gcccaagaa 
agttcaatt aacgataat ttttctggc cccgaatcg 
cgttttgag tgccccatt tgtctcgca tggctgcgt 
gatcccgtc ttgacttct tgtgggcac agattttgt 
cgtagttgc atggactca tggctcgaa cgcgaaaat 
ccctcgtgt cctatcgat aatacgaaa ctggaaaag 
ggagttgac attttcccc gataatttt actcgaaga 
gagatttca caacaaaaa accaaatgt ccgaatctc 
gtaaggggc tgtttagag gagttgtcg cctcttgat 
gttgaaaca catttactg acgtgtcag tataagcca 
ccggaactg cccgaaaat gagaaattg cgatgcgct 
tttgtcgaa ttcggctgc gaagataag tttgaagac 
gaaccggaa ttgcaaaga cacttggaa caatacata 
cacaaacat ttgaacctg ttgtcacaa gcatatagt 
aagttcaca ataaacagt ccctcaaca tcagcaatt 
gcacaacaa gcgaatttt tgggatccc ccgtctaaa 
aatgatact tcagtctca agtaatcac gataatgta 
aatgggtta ttaaaggcc ttgtatgag aaagccgtt 
ttattggaa caaaagaca cgcgaacaa gatataatc 
atagccaat atgtctgaa caaatttcg tctttgaat 
ctatcaatg tcaaaaatg cacttgcgt tactgtaac 
ggttgttat ttgtggtat tttaatgat ttcaaaaac 
aagataaat gcgatgcga aatgattcg agaattatg 
cactacagt cccggtttt tacaccgca gctaatgga 
tatagattg tgcattcgc ttgaattta tcacccaaa 
gacccaaat tacatagct atcttaatc cacgtgatg 
aaaacagag cacgacaat gccttagac tggcctttc 
agtggtaga ttaactata actttagtc catcctttg 
cagcctttt cacaacata aaagaaacg atgatgact 
cgtcccgaa ttggatgcg tttaagaaa ccagtgcaa 
gatatgaat cccaaaggt tttggttat accgaattc 
gccttaatt gaagaactc ttctctcaa ggctacgtc 
gaaaacaat catttgttg ataaaagtc caagctcaa 
cctgtgtaa
    mRNA sequence
    • >TC007706 RA
      aagggttaa taattgcaa aaagtttta tctggggac 
acgataaca tttattatt atgggtttc taagaaaac 
ctataattt cttcatatt ttggattta tagagacgg 
aagtgaata gtccgcttg aaaaagaag aatttactc 
ccttcagaa gtgaccaaa tagaagact ggtattatg 
gttataaat taatcaata aaaaattag gaatataaa 
tattatccg cttttttaa agttagaag cttaatttt 
aaattaatt ctttaattg ggattttta catcttact 
tatgacact atactcctc attactacc ataataatt 
gaataatga ataaatttg taaataaaa aaatagttc 
ccacaatat tcttttatt ctcttattc catttataa 
tttattctc aggtgtggt cacgtgact tagaaccac 
ctgtcaacc tgaaaatta actcagcag aaaacaatg 
ttgtgttga ttcacacgt gccaaaaat gtcttcagc 
ccacagtaa caccctggc ccaagaaag ttcaattaa 
cgataattt ttctggccc cgaatcgcg ttttgagtg 
ccccatttg tctcgcatg gctgcgtga tcccgtctt 
gacttcttg tgggcacag attttgtcg tagttgcat 
ggactcatg gctcgaacg cgaaaatcc ctcgtgtcc 
tatcgataa tacgaaact ggaaaaggg agttgacat 
tttccccga taattttac tcgaagaga gatttcaca 
acaaaaaac caaatgtcc gaatctcgt aaggggctg 
tttagagga gttgtcgcc tcttgatgt tgaaacaca 
tttactgac gtgtcagta taagccacc ggaactgcc 
cgaaaatga gaaattgcg atgcgcttt tgtcgaatt 
cggctgcga agataagtt tgaagacga accggaatt 
gcaaagaca cttggaaca atacataca caaacattt 
gaacctgtt gtcacaagc atatagtaa gttcacaat 
aaacagtcc ctcaacatc agcaattgc acaacaagc 
gaatttttg ggatccccc gtctaaaaa tgatacttc 
agtctcaag taatcacga taatgtaaa tgggttatt 
aaaggcctt gtatgagaa agccgtttt attggaaca 
aaagacacg cgaacaaga tataatcat agccaatat 
gtctgaaca aatttcgtc tttgaatct atcaatgtc 
aaaaatgca cttgcgtta ctgtaacgg ttgttattt 
gtggtattt taatgattt caaaaacaa gataaatgc 
gatgcgaaa tgattcgag aattatgca ctacagtcc 
cggttttta caccgcagc taatggata tagattgtg 
cattcgctt gaatttatc acccaaaga cccaaatta 
catagctat cttaatcca cgtgatgaa aacagagca 
cgacaatgc cttagactg gcctttcag tggtagatt 
aactataac tttagtcca tcctttgca gccttttca 
caacataaa agaaacgat gatgactcg tcccgaatt 
ggatgcgtt taagaaacc agtgcaaga tatgaatcc 
caaaggttt tggttatac cgaattcgc cttaattga 
agaactctt ctctcaagg ctacgtcga aaacaatca 
tttgttgat aaaagtcca agctcaacc tgtgtaaat 
aaaatcttt ttaaaatgg tttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY