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Gene information
General information
TC007706 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 32536..34463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007706
    iBeetle screen iB_09836

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages segmentation/division present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head potentially transformed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax potentially transformed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen potentially multiplied - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007706 RA
      MSSAHSNTL AQESSINDN FSGPESRFE CPICLAWLR 
      DPVLTSCGH RFCRSCMDS WLERENPSC PIDNTKLEK 
      GVDIFPDNF TRREISQQK TKCPNLVRG CLEELSPLD 
      VETHLLTCQ YKPPELPEN EKLRCAFVE FGCEDKFED 
      EPELQRHLE QYIHKHLNL LSQAYSKFT INSPSTSAI 
      AQQANFWDP PSKNDTSVS SNHDNVNGL LKALYEKAV 
      LLEQKTREQ DIIIANMSE QISSLNLSM SKMHLRYCN 
      GCYLWYFND FKNKINAMR NDSRIMHYS PGFYTAANG 
      YRLCIRLNL SPKDPNYIA ILIHVMKTE HDNALDWPF 
      SGRLTITLV HPLQPFHNI KETMMTRPE LDAFKKPVQ 
      DMNPKGFGY TEFALIEEL FSQGYVENN HLLIKVQAQ 
      PV
    CDS sequence
    • >TC007706 RA
      atgtcttca gcccacagt aacaccctg gcccaagaa 
      agttcaatt aacgataat ttttctggc cccgaatcg 
      cgttttgag tgccccatt tgtctcgca tggctgcgt 
      gatcccgtc ttgacttct tgtgggcac agattttgt 
      cgtagttgc atggactca tggctcgaa cgcgaaaat 
      ccctcgtgt cctatcgat aatacgaaa ctggaaaag 
      ggagttgac attttcccc gataatttt actcgaaga 
      gagatttca caacaaaaa accaaatgt ccgaatctc 
      gtaaggggc tgtttagag gagttgtcg cctcttgat 
      gttgaaaca catttactg acgtgtcag tataagcca 
      ccggaactg cccgaaaat gagaaattg cgatgcgct 
      tttgtcgaa ttcggctgc gaagataag tttgaagac 
      gaaccggaa ttgcaaaga cacttggaa caatacata 
      cacaaacat ttgaacctg ttgtcacaa gcatatagt 
      aagttcaca ataaacagt ccctcaaca tcagcaatt 
      gcacaacaa gcgaatttt tgggatccc ccgtctaaa 
      aatgatact tcagtctca agtaatcac gataatgta 
      aatgggtta ttaaaggcc ttgtatgag aaagccgtt 
      ttattggaa caaaagaca cgcgaacaa gatataatc 
      atagccaat atgtctgaa caaatttcg tctttgaat 
      ctatcaatg tcaaaaatg cacttgcgt tactgtaac 
      ggttgttat ttgtggtat tttaatgat ttcaaaaac 
      aagataaat gcgatgcga aatgattcg agaattatg 
      cactacagt cccggtttt tacaccgca gctaatgga 
      tatagattg tgcattcgc ttgaattta tcacccaaa 
      gacccaaat tacatagct atcttaatc cacgtgatg 
      aaaacagag cacgacaat gccttagac tggcctttc 
      agtggtaga ttaactata actttagtc catcctttg 
      cagcctttt cacaacata aaagaaacg atgatgact 
      cgtcccgaa ttggatgcg tttaagaaa ccagtgcaa 
      gatatgaat cccaaaggt tttggttat accgaattc 
      gccttaatt gaagaactc ttctctcaa ggctacgtc 
      gaaaacaat catttgttg ataaaagtc caagctcaa 
      cctgtgtaa
    mRNA sequence
    • >TC007706 RA
      aagggttaa taattgcaa aaagtttta tctggggac 
      acgataaca tttattatt atgggtttc taagaaaac 
      ctataattt cttcatatt ttggattta tagagacgg 
      aagtgaata gtccgcttg aaaaagaag aatttactc 
      ccttcagaa gtgaccaaa tagaagact ggtattatg 
      gttataaat taatcaata aaaaattag gaatataaa 
      tattatccg cttttttaa agttagaag cttaatttt 
      aaattaatt ctttaattg ggattttta catcttact 
      tatgacact atactcctc attactacc ataataatt 
      gaataatga ataaatttg taaataaaa aaatagttc 
      ccacaatat tcttttatt ctcttattc catttataa 
      tttattctc aggtgtggt cacgtgact tagaaccac 
      ctgtcaacc tgaaaatta actcagcag aaaacaatg 
      ttgtgttga ttcacacgt gccaaaaat gtcttcagc 
      ccacagtaa caccctggc ccaagaaag ttcaattaa 
      cgataattt ttctggccc cgaatcgcg ttttgagtg 
      ccccatttg tctcgcatg gctgcgtga tcccgtctt 
      gacttcttg tgggcacag attttgtcg tagttgcat 
      ggactcatg gctcgaacg cgaaaatcc ctcgtgtcc 
      tatcgataa tacgaaact ggaaaaggg agttgacat 
      tttccccga taattttac tcgaagaga gatttcaca 
      acaaaaaac caaatgtcc gaatctcgt aaggggctg 
      tttagagga gttgtcgcc tcttgatgt tgaaacaca 
      tttactgac gtgtcagta taagccacc ggaactgcc 
      cgaaaatga gaaattgcg atgcgcttt tgtcgaatt 
      cggctgcga agataagtt tgaagacga accggaatt 
      gcaaagaca cttggaaca atacataca caaacattt 
      gaacctgtt gtcacaagc atatagtaa gttcacaat 
      aaacagtcc ctcaacatc agcaattgc acaacaagc 
      gaatttttg ggatccccc gtctaaaaa tgatacttc 
      agtctcaag taatcacga taatgtaaa tgggttatt 
      aaaggcctt gtatgagaa agccgtttt attggaaca 
      aaagacacg cgaacaaga tataatcat agccaatat 
      gtctgaaca aatttcgtc tttgaatct atcaatgtc 
      aaaaatgca cttgcgtta ctgtaacgg ttgttattt 
      gtggtattt taatgattt caaaaacaa gataaatgc 
      gatgcgaaa tgattcgag aattatgca ctacagtcc 
      cggttttta caccgcagc taatggata tagattgtg 
      cattcgctt gaatttatc acccaaaga cccaaatta 
      catagctat cttaatcca cgtgatgaa aacagagca 
      cgacaatgc cttagactg gcctttcag tggtagatt 
      aactataac tttagtcca tcctttgca gccttttca 
      caacataaa agaaacgat gatgactcg tcccgaatt 
      ggatgcgtt taagaaacc agtgcaaga tatgaatcc 
      caaaggttt tggttatac cgaattcgc cttaattga 
      agaactctt ctctcaagg ctacgtcga aaacaatca 
      tttgttgat aaaagtcca agctcaacc tgtgtaaat 
      aaaatcttt ttaaaatgg tttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY