Status
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Gene information
General information
TC007709 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 54025..58131
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007709
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09835

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007709 RA
      MPSASNVKI DKQVSPEAT LPDSSNSTP LRQVITLVE 
      HKIRNLEKR KSKLESYRD LQNAGKELN SDQKTAVAK 
      YNEVTQTLD FARDIYKQF LGIAVVSEK EAKKQAKKE 
      AASKSQAEL ARLREILLV QDALNQMGN EAVREDFLH 
      GRNGAAQLT EADLKLLDD LYPAVTPKH EAGNPTAFT 
      NEVQAAAEH LLAVVDGKP KEVFGGTYS QVKEILGKI 
      HESGYFDQV PVFENLAES EQPEAPTVE AETSQQLPP 
      MEMLSIESL PPTMIQQPE QPPMEQPLT PEQQMFYQQ 
      QQQQQQQQQ QQPPRPITE MLGSGSFFF LQESEIDTP 
      PEQIPSQTF TNQSFVTAA PPPPIPLPP LFQNIPTNL 
      PPPPPPQQP ITVNNVEEV HKEEVHKQG GNRPPRPNN 
      QSYYQNNNG YSNRPKQNR NGPRPANRH
    CDS sequence
    • >TC007709 RA
      atgccctcg gcctcgaat gtgaaaatt gataagcaa 
      gtgtctcca gaagcaact cttccggac tcatcgaat 
      tcgacgccc ttgagacaa gtcataacc ctcgtagaa 
      cacaaaatt aggaacctc gaaaagcgc aagtcaaaa 
      ttagaatca tatagagat ctacaaaac gccggtaaa 
      gaacttaac tccgaccag aagactgcc gtagccaag 
      tacaatgag gtgacccag acgttggac tttgccaga 
      gatatctac aaacagttc ctaggaatc gcagtcgtc 
      tcggaaaag gaggccaag aaacaggcg aagaaagaa 
      gcggcctcc aaaagtcaa gctgaattg gcgagacta 
      cgggagatt cttttggtg caagatgca cttaatcaa 
      atgggaaat gaagcggtt agggaggac tttctccat 
      gggaggaac ggcgccgcg cagctcacc gaggccgac 
      ctcaaactg ttggacgac ttgtatcct gctgtgacc 
      ccaaaacac gaagcggga aacccaact gcattcacg 
      aatgaggtt caagccgcc gccgaacac ctccttgcc 
      gtcgtcgac ggcaaaccc aaggaagtg tttggagga 
      acttacagt caagttaaa gaaattctt ggcaaaatt 
      cacgaaagt ggttatttt gatcaagtt cctgttttc 
      gagaatttg gcggaaagc gaacaaccg gaagctcca 
      actgtcgaa gctgaaact tcccagcaa cttccacct 
      atggagatg ctttccatt gagagtcta ccaccaaca 
      atgatacaa caaccggag caaccgcca atggaacag 
      cctcttact cccgaacag caaatgttt tatcaacaa 
      caacaacag cagcagcag caacaacaa caacagcct 
      cctagaccc ataacggaa atgctaggg agtggaagt 
      ttcttcttt ttgcaagag tctgaaatt gacactcca 
      cctgaacag attccgagc caaacgttt acgaatcag 
      agttttgtg acagcagcg cccccacca cctattcct 
      ttgcctcct ttattccag aatattcca acgaattta 
      ccaccaccc ccaccaccg caacaacca atcacggtg 
      aataatgtc gaagaggtc cataaagaa gaggtgcat 
      aaacaagga gggaatcgc ccaccgaga cccaataat 
      caatcgtat tatcagaat aataacgga tacagtaac 
      aggcctaaa caaaaccgg aacggacct agacctgca 
      aataggcat taa
    mRNA sequence
    • >TC007709 RA
      tatagtttt tgaaattga aataatcac aaaaaatga 
      ttttacttt aaacatcct gtaaaattt agccaagat 
      taggtacat attttttta ttagtggtg gttatttgc 
      cattgattt tcttaacaa cttgtaaag agaaagtga 
      tattgttta ttacattat tacgatact agtggttgt 
      gcaaccatc gcaccattc gtaataaat catttttga 
      cattatttt ctggtgttt aaatttccc gcttcgttt 
      cttcaaatc cggcgtaat tgaccaatc gcgttaaag 
      taatggcga tttccggca gtgtcggct tttcgatgg 
      agtgatcac tttatgttg tcaatcttg aacgtttct 
      tgcgttatt tcgttttaa aaacttcat aaaaacatt 
      aaatcctac ttgaataag taaggaact cttggagtg 
      tttagttta actcgctca aaaatgccc tcggcctcg 
      aatgtgaaa attgataag caagtgtct ccagaagca 
      actcttccg gactcatcg aattcgacg cccttgaga 
      caagtcata accctcgta gaacacaaa attaggaac 
      ctcgaaaag cgcaagtca aaattagaa tcatataga 
      gatctacaa aacgccggt aaagaactt aactccgac 
      cagaagact gccgtagcc aagtacaat gaggtgacc 
      cagacgttg gactttgcc agagatatc tacaaacag 
      ttcctagga atcgcagtc gtctcggaa aaggaggcc 
      aagaaacag gcgaagaaa gaagcggcc tccaaaagt 
      caagctgaa ttggcgaga ctacgggag attcttttg 
      gtgcaagat gcacttaat caaatggga aatgaagcg 
      gttagggag gactttctc catgggagg aacggcgcc 
      gcgcagctc accgaggcc gacctcaaa ctgttggac 
      gacttgtat cctgctgtg accccaaaa cacgaagcg 
      ggaaaccca actgcattc acgaatgag gttcaagcc 
      gccgccgaa cacctcctt gccgtcgtc gacggcaaa 
      cccaaggaa gtgtttgga ggaacttac agtcaagtt 
      aaagaaatt cttggcaaa attcacgaa agtggttat 
      tttgatcaa gttcctgtt ttcgagaat ttggcggaa 
      agcgaacaa ccggaagct ccaactgtc gaagctgaa 
      acttcccag caacttcca cctatggag atgctttcc 
      attgagagt ctaccacca acaatgata caacaaccg 
      gagcaaccg ccaatggaa cagcctctt actcccgaa 
      cagcaaatg ttttatcaa caacaacaa cagcagcag 
      cagcaacaa caacaacag cctcctaga cccataacg 
      gaaatgcta gggagtgga agtttcttc tttttgcaa 
      gagtctgaa attgacact ccacctgaa cagattccg 
      agccaaacg tttacgaat cagagtttt gtgacagca 
      gcgccccca ccacctatt cctttgcct cctttattc 
      cagaatatt ccaacgaat ttaccacca cccccacca 
      ccgcaacaa ccaatcacg gtgaataat gtcgaagag 
      gtccataaa gaagaggtg cataaacaa ggagggaat 
      cgcccaccg agacccaat aatcaatcg tattatcag 
      aataataac ggatacagt aacaggcct aaacaaaac 
      cggaacgga cctagacct gcaaatagg cattaaatc 
      atgacgcaa gcgaaattc gatattagt ctcttattt 
      aacttaatt attatgatt aaaaaaaca taagtcact 
      aaagtcaat tggttcaac tcaaatcgg gtttttttt 
      ttggttggc catcgaata tttcatcat gctttaata 
      aatatgtta taaaataaa aaacggcac aattgtgtt 
      aattaaact tgtcgagtg gcattttca gtttttact 
      tctttttct actctacct tttcagtat gtttagaaa 
      tttttagta ttgaatttt atcgaaatt aattaattg 
      gattaataa aagctggta aaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY