Status
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Gene information
General information
TC007711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 70155..72154
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007711
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09834

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007711 RA
      MASSTSSTT SSLQKWQIA LGIGAVSAL GLSYWYLKT 
      GRKPQLKDT SDTISIDES VKDPPKDPT PLEQAQQYK 
      TSGNEMFKK GKFDEAIHL YNKAIEACP EEFKTELAT 
      YYQNRAAAY ENLKKWSSV IADCTKAIE LNSRYEKAL 
      MRRAKAEEI VKDWENCLD DVTCVCLLQ QFQNQTALL 
      MADRVLKEL GKKHAQEAM LNRKPIIPS KTFIKTYFS 
      SFSEDVVYQ KLTEVGDPL GQGELSGFL KAKLAFATE 
      KYEEIIPSC TEEINSSES ESPFKIEAL SLRASFYFL 
      TGNLDKALE DLTTIINTD CDVKIKVNS LIKRASIYM 
      QNEKLTECL EDFDKAAEV GPEISDVFH HRGQVKLLM 
      EKTNEAILD FQKAVDLNP NFSVAFVQK CYSDYRHAM 
      QVQDVQLLM QSLANFKKG IEKFPTCVE TYVLYAQVL 
      TEKQDYEEA DKLYGKALE IDSENASIY VHRGLLMLQ 
      WKGEIEQAV ELMKEGIKI DDKCEFAYE TLGTVEVQR 
      GNLVVAIDL FNKAIALAR SEMELTHLF SLRDAATSQ 
      LKVTTRLGI DSQHMVNK
    CDS sequence
    • >TC007711 RA
      atggcctca tccacctct agcacaact tcatcgctc 
      caaaaatgg caaatcgct ctcggaatc ggagctgta 
      agcgctcta ggtctcagt tactggtac ttaaaaacc 
      ggccgaaag ccccaattg aaagacacc agcgataca 
      atatcaatc gatgagtcc gtcaaagat ccccctaag 
      gaccccacc cctttggag caagcccaa cagtataaa 
      acttccgga aacgaaatg ttcaaaaaa ggcaaattt 
      gacgaagcc atccacttg tacaacaaa gccatcgag 
      gcatgtccg gaagaattc aaaacggaa ttagcgacg 
      tattatcaa aatcgagca gcggcttac gaaaacctt 
      aaaaaatgg agcagtgta attgctgat tgtaccaaa 
      gcgattgaa ttgaattcg agatacgaa aaggcgtta 
      atgaggaga gccaaggct gaggaaatt gtcaaagac 
      tgggagaat tgtttggat gacgtcacg tgtgtgtgt 
      cttttgcaa caattccag aatcaaacg gcactcttg 
      atggctgat cgcgttttg aaagagtta ggtaaaaaa 
      cacgcacag gaggccatg cttaaccga aaacctata 
      attccgtca aagactttt atcaagact tatttttcg 
      tcgttttct gaggatgtg gtttatcag aaattgact 
      gaagtgggg gatccactc ggccaagga gagctttcg 
      ggtttcctc aaagccaaa ctcgctttc gctacggaa 
      aaatacgaa gaaataatc ccatcttgt accgaagaa 
      atcaattca agtgaatcc gaatcgccg tttaaaatc 
      gaagcttta tcactccga gcttcgttt tattttctc 
      actggaaat ctagacaaa gcacttgaa gacttgacc 
      acgatcata aacaccgac tgcgatgtt aaaattaaa 
      gttaattca ctaatcaag cgtgcgtcg atttacatg 
      caaaatgag aagctcaca gagtgcctt gaggatttc 
      gataaagcg gctgaagtc ggcccggaa atttccgat 
      gttttccat catcgaggt caagtcaag cttttgatg 
      gaaaaaaca aatgaggcg atactcgat tttcagaaa 
      gctgttgat ttaaatccg aatttttcg gtggctttt 
      gtacaaaaa tgctattcg gattatcgg cacgcaatg 
      caagtgcag gacgtgcaa cttttgatg caaagtttg 
      gccaatttc aagaaaggc attgaaaaa ttcccaact 
      tgtgtcgag acttacgtt ttgtacgcg caagttttg 
      accgagaaa caggattat gaagaggca gataagttg 
      tatggaaaa gcgctagag attgactcg gaaaatgcc 
      tcgatttac gtacatcga ggtcttttg atgttgcaa 
      tggaaaggt gagattgaa caagcggtc gaattgatg 
      aaagaagga atcaaaatt gatgataaa tgcgaattt 
      gcctatgaa actttaggg acagttgag gttcaaaga 
      ggaaattta gtcgttgcg atcgattta ttcaacaaa 
      gctatagcg ttggcacga tctgaaatg gaactcact 
      catttgttt tcgctcaga gatgcagca acgtcgcaa 
      ttaaaagtc actacacga ctagggatt gattcgcag 
      catatggtt aacaaataa
    mRNA sequence
    • >TC007711 RA
      acaaaatca agtaaaaaa aaatccagc agctgtcaa 
      tttgttggc tgtcaaatc tgttacata acctcattt 
      tcgataaat aaccaattc aagataaga accccgtgt 
      gtgtgtgtc caactatcc gtggtagtg taaaatcga 
      tggcctcat ccacctcta gcacaactt catcgctcc 
      aaaaatggc aaatcgctc tcggaatcg gagctgtaa 
      gcgctctag gtctcagtt actggtact taaaaaccg 
      gccgaaagc cccaattga aagacacca gcgatacaa 
      tatcaatcg atgagtccg tcaaagatc cccctaagg 
      accccaccc ctttggagc aagcccaac agtataaaa 
      cttccggaa acgaaatgt tcaaaaaag gcaaatttg 
      acgaagcca tccacttgt acaacaaag ccatcgagg 
      catgtccgg aagaattca aaacggaat tagcgacgt 
      attatcaaa atcgagcag cggcttacg aaaacctta 
      aaaaatgga gcagtgtaa ttgctgatt gtaccaaag 
      cgattgaat tgaattcga gatacgaaa aggcgttaa 
      tgaggagag ccaaggctg aggaaattg tcaaagact 
      gggagaatt gtttggatg acgtcacgt gtgtgtgtc 
      ttttgcaac aattccaga atcaaacgg cactcttga 
      tggctgatc gcgttttga aagagttag gtaaaaaac 
      acgcacagg aggccatgc ttaaccgaa aacctataa 
      ttccgtcaa agactttta tcaagactt atttttcgt 
      cgttttctg aggatgtgg tttatcaga aattgactg 
      aagtggggg atccactcg gccaaggag agctttcgg 
      gtttcctca aagccaaac tcgctttcg ctacggaaa 
      aatacgaag aaataatcc catcttgta ccgaagaaa 
      tcaattcaa gtgaatccg aatcgccgt ttaaaatcg 
      aagctttat cactccgag cttcgtttt attttctca 
      ctggaaatc tagacaaag cacttgaag acttgacca 
      cgatcataa acaccgact gcgatgtta aaattaaag 
      ttaattcac taatcaagc gtgcgtcga tttacatgc 
      aaaatgaga agctcacag agtgccttg aggatttcg 
      ataaagcgg ctgaagtcg gcccggaaa tttccgatg 
      ttttccatc atcgaggtc aagtcaagc ttttgatgg 
      aaaaaacaa atgaggcga tactcgatt ttcagaaag 
      ctgttgatt taaatccga atttttcgg tggcttttg 
      tacaaaaat gctattcgg attatcggc acgcaatgc 
      aagtgcagg acgtgcaac ttttgatgc aaagtttgg 
      ccaatttca agaaaggca ttgaaaaat tcccaactt 
      gtgtcgaga cttacgttt tgtacgcgc aagttttga 
      ccgagaaac aggattatg aagaggcag ataagttgt 
      atggaaaag cgctagaga ttgactcgg aaaatgcct 
      cgatttacg tacatcgag gtcttttga tgttgcaat 
      ggaaaggtg agattgaac aagcggtcg aattgatga 
      aagaaggaa tcaaaattg atgataaat gcgaatttg 
      cctatgaaa ctttaggga cagttgagg ttcaaagag 
      gaaatttag tcgttgcga tcgatttat tcaacaaag 
      ctatagcgt tggcacgat ctgaaatgg aactcactc 
      atttgtttt cgctcagag atgcagcaa cgtcgcaat 
      taaaagtca ctacacgac tagggattg attcgcagc 
      atatggtta acaaataat tgttagttg ttgtattaa 
      attgtaccc ctccttaaa ttttttagt gtaaatatg 
      gcattccag acaatatcg caaaaattt tagtgtatt 
      aatttcaaa caggtgctt aataaacca tttctatcc 
      aa
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