Status
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Gene information
General information
TC007781 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 84601..89333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007781
    iBeetle screen iB_09833

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_10512

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007781 RA
      MDNIRNWYK IPRALFRLC FILVNNVYC IPTYVVWMV 
      LLSPLRKVN PDVYWRIEG YFFHWLLAM VSMWSWSAG 
      YDVVEMGDD IAKCIDDRT LVIANHQST GDVPLLMAT 
      FNPRKQILP NIMWIMDSL FKYTNFGIV SVLHQDFFI 
      MSGKSKRDK SLQALVKHL QDSYLPCNR KWMVLFPEG 
      GFLRKRKAV SQKYALKNG LPMLNNVSL PRVGAMHSI 
      METVGPKAN NNASSGTEI STLRWILDI TIAYPKGEP 
      IDLAAIVFG HRPPCKTFL FYRLYQCKD LPEDSESLT 
      SWLFERWQE KERMLESFY QTGEFPGYS GSGHLVEQD 
      YLRYVILHL FFIISTYIH VQIFQAAYH YCTCLIY
    CDS sequence
    • >TC007781 RA
      atggataat atacggaat tggtacaag ataccgaga 
      gcgttgttc aggctgtgt tttattcta gttaacaat 
      gtctattgt attcccacg tacgttgta tggatggta 
      ttgttgtca ccccttcgt aaagtcaat cctgacgtt 
      tattggagg atagaaggg tattttttc cattggtta 
      ctggcgatg gtatcaatg tggagttgg tcagcagga 
      tatgatgtc gtcgaaatg ggcgacgat attgcaaaa 
      tgtatagac gatcgaacg ttagtgata gccaatcat 
      caatcaacc ggtgatgtt cccctttta atggcaaca 
      ttcaatccc cgaaaacag atccttccg aatataatg 
      tggatcatg gatagttta ttcaaatac acaaatttc 
      ggtatagtc agtgtcctc catcaagat tttttcata 
      atgtcgggt aaaagtaag cgtgacaag tcgttacaa 
      gcccttgtc aagcatttg caagactct tatttgcct 
      tgtaatcgc aaatggatg gtgttattc cccgaaggt 
      ggttttttg cgtaaacga aaagccgtt tcacagaaa 
      tatgcactg aaaaatgga ctaccaatg ttaaataat 
      gtgtcattg ccacgagtt ggagcaatg cattcgata 
      atggagaca gtggggcct aaagctaat aataacgca 
      tcatcaggg acggaaatt tcgacactg cgttggatt 
      ctggatata acgatagcg tatccaaag ggtgaaccg 
      atcgatttg gccgcgata gtgttcggg caccggccc 
      ccatgcaag acatttctt ttctaccgt ttgtatcag 
      tgtaaagac ttgccagaa gacagcgaa agtttgaca 
      agttggttg tttgagcgt tggcaagag aaagaacga 
      atgttggag agtttttat caaactggt gaatttccg 
      ggttattcc ggaagcggg catctagtc gaacaagac 
      tatttacgt tacgtcatt ttgcacttg tttttcata 
      atttcgacg tacattcat gtgcagatt ttccaagca 
      gcttatcat tattgcact tgtttgatt tactag
    mRNA sequence
    • >TC007781 RA
      gttaatgca aaaagcaca ccatttgca ccataatgg 
      acttttaaa caaacaaaa aaaaacagg tataccttt 
      aataaaaat tgatgatgg taaacttcg aacgtgtga 
      ttcattcga aatgtgttt tttccactg ggaaaattc 
      gattttccg gtcacaaat tttgcacaa aagtaaaac 
      cattttcag ggttagaaa gtcaaaaat cgtttcatt 
      ttaatatgc aaaacctgg aaaatcaca ttttgcttc 
      aagtttagc aataatact tacaaatca cttactatt 
      cgcttctta ttccttttt ttctcacat tttaatcac 
      ctcatacca aaattgttt atattctgt tcaagtgtc 
      agccgtttt ttggcgtta caaccaaac gtcacattt 
      tatcgacaa ataaataac tcggaaaaa ataaatact 
      aaaccatca taattacac tagatttgt acgatgacg 
      caacgtgtt ttgtacaaa agatatgac acaagagtt 
      tggaaaaaa aattaattg atttgataa tttgaccag 
      atcgaacgc gcaaatgtg ccggcaaag tgtgtctaa 
      aggcacacc caatttatt ttaatccga attttggta 
      ttaccgaca ctctgacgt cattttttg ctggttttg 
      agtcactta aatgcacta ttgatgatt tataaattc 
      ctagcatta aaatcggcc catttaaac caaaattct 
      ttgttcaaa tgatgtaac cacaaccac tcatgttcc 
      ctccaaatt aaattgagg ttatattcg attatttat 
      ttacttttt ttataaatg gatgataat ccgaaagca 
      aggggacgg aaacaccgt tcaaacaaa aacaagtac 
      aaaccggga ggggctcat gcgccccct cacaaaaca 
      aggccccca tctttagaa tccaattgg gaacgctat 
      gagcaagaa aagggtttg aaaagtagt cctggaagt 
      gacttttcc gttctagct aacgcccca atttcccat 
      gggagtcat tttcaattt aaaagcgat aaagacatt 
      gcgttagaa attaaagag gcgtctgaa ttgtttaat 
      ttagacttg gctttgtta gataagagt ctagggact 
      atttcgttt cagctcagg tcaggcatt gaaaacgac 
      tatttcaca atattttgc caaaatcaa aaaaataat 
      attgatgat tactcagtt tatgtcaag ggttatcgt 
      ccaagactt gtttttggg tcagacgat gagcggaac 
      tcacccacg cctgaatca agtaattta ataattaat 
      gcaaggatt actgtagat atgtgactt gtaggtgtg 
      tcaccactg caccttgag cctccgaag tccgatgaa 
      gacaacttg gaagcctgg cttgacaat attttggac 
      aattaatta ttatttatt atcaatata aataaaatt 
      taaatttgg cgccttgta tccagatgg agtcccaat 
      cgtttgtca ctttgacat ttacgagca cgtcaaatt 
      tgtcattgt tatcggatc atttttcta ttttcatta 
      ttagtgaaa tcgttatgt gagaccggt gcccaaaat 
      ttcacccgc aagctcgaa attagcgca aattatgtt 
      gattttttc gcgttcggc tgtcataac cacaaaaat 
      tcgaatttc gaaacgaat aatggatag aattatcac 
      tcggagtgt ttaaataaa gtcaattcg tagtgcaat 
      tgcattttt gtttattta ttcaagcca ttagcgtgc 
      atgaaccat cgattattt tatttttat acctataat 
      acgcgcaca aaagtggta gctttttcg tagaatgaa 
      acaaaaaaa tagtgaata aaaatggat aatatacgg 
      aattggtac aagataccg agagcgttg ttcaggctg 
      tgttttatt ctagttaac aatgtctat tgtattccc 
      acgtacgtt gtatggatg gtattgttg tcacccctt 
      cgtaaagtc aatcctgac gtttattgg aggatagaa 
      gggtatttt ttccattgg ttactggcg atggtatca 
      atgtggagt tggtcagca ggatatgat gtcgtcgaa 
      atgggcgac gatattgca aaatgtata gacgatcga 
      acgttagtg atagccaat catcaatca accggtgat 
      gttcccctt ttaatggca acattcaat ccccgaaaa 
      cagatcctt ccgaatata atgtggatc atggatagt 
      ttattcaaa tacacaaat ttcggtata gtcagtgtc 
      ctccatcaa gattttttc ataatgtcg ggtaaaagt 
      aagcgtgac aagtcgtta caagccctt gtcaagcat 
      ttgcaagac tcttatttg ccttgtaat cgcaaatgg 
      atggtgtta ttccccgaa ggtggtttt ttgcgtaaa 
      cgaaaagcc gtttcacag aaatatgca ctgaaaaat 
      ggactacca atgttaaat aatgtgtca ttgccacga 
      gttggagca atgcattcg ataatggag acagtgggg 
      cctaaagct aataataac gcatcatca gggacggaa 
      atttcgaca ctgcgttgg attctggat ataacgata 
      gcgtatcca aagggtgaa ccgatcgat ttggccgcg 
      atagtgttc gggcaccgg cccccatgc aagacattt 
      cttttctac cgtttgtat cagtgtaaa gacttgcca 
      gaagacagc gaaagtttg acaagttgg ttgtttgag 
      cgttggcaa gagaaagaa cgaatgttg gagagtttt 
      tatcaaact ggtgaattt ccgggttat tccggaagc 
      gggcatcta gtcgaacaa gactattta cgttacgtc 
      attttgcac ttgtttttc ataatttcg acgtacatt 
      catgtgcag attttccaa gcagcttat cattattgc 
      acttgtttg atttactag cgttccgtt ctcgcatat 
      ctaatctgt ttggtgcgt ttgtatctg tgacagatt 
      ggattgatg cgagttgga tattgtact taatttttt 
      ttaccggaa aattgggta aataataat aaaacacgg 
      aacaatctt gccttgtgt ttatgacga cgtaatcat 
      acaataata agcgtagat tttgtacag tagtttagg 
      accaattgt gcattttgt gtttaggtt gattttttt 
      ctctcacgg cgaaatttg aggctcaat cgttgacga 
      cgtccattt tttatgaag atattttag caccgcact 
      catctcgat tttttaatt cgcttgtgt tttaaattt 
      ttcaaggtt aacttatta tgtatacat atttttgta 
      aatttcaaa gtttctttg taataaaaa attcatgag 
      aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY