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Gene information
General information
TC007768 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 285056..287217
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007768
    iBeetle screen iB_09830

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007768 RA
      MELKVWVEG IQRIVCGVT ETTTCQDVV FALAHATGK 
      SGRFTLIER WRNNERQLA PNENPLKIL MKWGEYSND 
      VQFILQWSE SKPMTPTTK PKTSSSSAN IIVESPQKH 
      TEKSQVGVV KGVPHVKTL QVRESQNSP KKSESPSHR 
      IHPPAPPPY RDPPPPKKN ILQESNKTH KLQESVLYN 
      AQYRELIAL INYQREKLS NQQADLTKF DAEIVFWES 
      KEREKQMQL EYISQEILA MTNATKLNQ DQIQALHYI 
      EEECEIVKQ QEKTLKSEI TLLRSKLAN CETELLQCK 
      NKIRLVMDE LQIEQRALS RRSESRKQM EKSLLVEME 
      RLQRDIELA KQSTELHHL TAETLKKEV AALETAIIE 
      KKKQVELLV TEMKEANLQ SLTIAPPED LRAILEGPN 
      KGSTRKMIG SPRQLENAV PTNKNPHGV WV
    CDS sequence
    • >TC007768 RA
      atggagtta aaagtttgg gtcgaggga atccaacgg 
      atagtgtgt ggggtgact gagacgaca acgtgtcag 
      gatgtagtg tttgcttta gctcacgcc acgggtaag 
      tcaggccga ttcacactt atcgaacgg tggaggaac 
      aacgaacga cagttggcc cccaatgaa aaccccctc 
      aagattttg atgaaatgg ggtgagtac tcaaacgac 
      gtccaattc atcctccaa tggagcgaa agcaaaccc 
      atgacacca acaaccaaa ccaaaaaca tcatcatca 
      tcagctaat atcatagtg gaatcacca caaaaacac 
      accgagaag tcacaagta ggcgttgtg aaaggtgta 
      ccgcatgta aaaacacta caagtgcgc gaatctcaa 
      aattcaccc aaaaaatcc gaatctcct agtcataga 
      atccaccca ccggcaccg cccccttac cgcgatccg 
      ccgcccccc aaaaaaaat atcctacaa gagtcaaac 
      aaaacacac aaattacag gagtctgta ctctacaat 
      gcccagtat agggaatta atcgcgctt attaattac 
      cagagggag aaattgtcc aatcagcaa gcagaccta 
      acaaagttt gatgccgaa attgtgttt tgggagagt 
      aaagaacgg gaaaaacaa atgcaatta gaatacatt 
      tcgcaggaa attctcgca atgactaat gctacgaaa 
      ttaaatcaa gaccagatt caggcattg cattacatt 
      gaggaagaa tgcgaaata gtgaaacag caagagaaa 
      acgctcaaa tccgaaata actctcttg agatcaaag 
      cttgcaaat tgcgaaact gaacttttg cagtgcaaa 
      aataaaatt cgtttagta atggacgag ttacaaatc 
      gaacaaaga gctttatcg cgtcgttct gaatcacgc 
      aaacaaatg gaaaaaagt ctcctagtc gaaatggag 
      cggttacag agagatatc gagttggca aaacagagc 
      actgaattg catcacctg actgccgaa acgctcaaa 
      aaagaagtt gccgcactt gagacggca ataatcgaa 
      aagaaaaaa caagtggaa ttgcttgtg actgaaatg 
      aaagaggcg aatttacaa agtttgact atagcgcca 
      cctgaggat ttacgagcg attttggaa ggtcctaat 
      aagggaagt acccgaaaa atgattggt tcgcctcga 
      caattggaa aatgctgta ccaaccaat aaaaacccg 
      catggagtt tgggtctaa
    mRNA sequence
    • >TC007768 RA
      ggaaaagtc taaaaattg gcacaaaaa gacacaact 
      attacgcga cttttcttt cactttgaa tcaagttgg 
      cgggtttgg tgcttttga cccgttggg ttggtggca 
      agtgacaag agtaaaaaa tatttgacg tttataaat 
      aaaaataaa ttgcacatt tttgcccca aaacatgta 
      aaaaaatgc aaattttga ataaacgtc gctttaatt 
      tagttacgt aaactcggg gtttgttac caccgtgct 
      tcgattgat caaaatgcc aaaacctgg aattagtgc 
      aatatggag ttaaaagtt tgggtcgag ggaatccaa 
      cggatagtg tgtggggtg actgagacg acaacgtgt 
      caggatgta gtgtttgct ttagctcac gccacgggt 
      aagtcaggc cgattcaca cttatcgaa cggtggagg 
      aacaacgaa cgacagttg gcccccaat gaaaacccc 
      ctcaagatt ttgatgaaa tggggtgag tactcaaac 
      gacgtccaa ttcatcctc caatggagc gaaagcaaa 
      cccatgaca ccaacaacc aaaccaaaa acatcatca 
      tcatcagct aatatcata gtggaatca ccacaaaaa 
      cacaccgag aagtcacaa gtaggcgtt gtgaaaggt 
      gtaccgcat gtaaaaaca ctacaagtg cgcgaatct 
      caaaattca cccaaaaaa tccgaatct cctagtcat 
      agaatccac ccaccggca ccgccccct taccgcgat 
      ccgccgccc cccaaaaaa aatatccta caagagtca 
      aacaaaaca cacaaatta caggagtct gtactctac 
      aatgcccag tatagggaa ttaatcgcg cttattaat 
      taccagagg gagaaattg tccaatcag caagcagac 
      ctaacaaag tttgatgcc gaaattgtg ttttgggag 
      agtaaagaa cgggaaaaa caaatgcaa ttagaatac 
      atttcgcag gaaattctc gcaatgact aatgctacg 
      aaattaaat caagaccag attcaggca ttgcattac 
      attgaggaa gaatgcgaa atagtgaaa cagcaagag 
      aaaacgctc aaatccgaa ataactctc ttgagatca 
      aagcttgca aattgcgaa actgaactt ttgcagtgc 
      aaaaataaa attcgttta gtaatggac gagttacaa 
      atcgaacaa agagcttta tcgcgtcgt tctgaatca 
      cgcaaacaa atggaaaaa agtctccta gtcgaaatg 
      gagcggtta cagagagat atcgagttg gcaaaacag 
      agcactgaa ttgcatcac ctgactgcc gaaacgctc 
      aaaaaagaa gttgccgca cttgagacg gcaataatc 
      gaaaagaaa aaacaagtg gaattgctt gtgactgaa 
      atgaaagag gcgaattta caaagtttg actatagcg 
      ccacctgag gatttacga gcgattttg gaaggtcct 
      aataaggga agtacccga aaaatgatt ggttcgcct 
      cgacaattg gaaaatgct gtaccaacc aataaaaac 
      ccgcatgga gtttgggtc taataaaat tgtagtcat 
      tttgtttag cttacctca gtctaggcg tgtttgatt 
      ttcaagtct gatagtttg tattgacgc cattttaaa 
      ttataaaaa gtgttattt attgttagg tgattggat 
      gatttttta cttacacat ttttgttac tttatttta 
      ttttacact gccatattg tgtgtagtt gcatcacac 
      aggattatg ataaagaaa tgatgttag atatattta 
      ttattttaa aatattaaa attatattt aattgctat 
      gtcggaaat gtaaaatac acacaaaaa tattaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY