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Gene information
General information
TC004392 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7114853..7116610
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004392
    iBeetle screen iB_09829

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004392 RA
      MKPNQDDLT PAEINKQML EIKKKIQLS EGQRKAAFE 
      DCEAERKSN TDEIHKLKT EIRELVKKL KEKTQSTAA 
      LIKTYKKLE SLLGPIDDK STSTIEQLL DLQIIDKSK 
      CLNLVNYKI KKRQKLLTQ LGEEFQLLQ SEKSLQQVV 
      EKVDKPAKK ATCELQNSI HAVEVQLRE ANHISARYK 
      DVKKSLLRD SARFESNIK RIEEELALQ NNDITELQH 
      VMNEATKKR STARGHLLR EEKIAMSSA HFRDRQTVE 
      GQRLVTRRK QELELLEKR IFQVVKVAR VEEIIEEEN 
      ETMLTTLSP PEEVLTRAF EVLKKATGA TNTSEVLHK 
      FNMQKDTLE RLILLRKKS ESEKKQLEK NLDDLNTQL 
      EFFKYAEVK ETERKSLKM EQMLSQIDE IKNISLKTN 
      NLKLVKDKT IQTILRSVQ ELQACINPI SVPESNVLG 
      TLECIKSDL AKIIRIENE ENVVKLEDI EEKWLPGPY 
      SGLVRRTPI PQPGTSPAP PPPPTGSED EEEVPSRTY 
      LKRQAQLVV DAKSRRKNI RIPAKK
    CDS sequence
    • >TC004392 RA
      atgaagccg aatcaggat gatttaacc ccagccgag 
      atcaataaa caaatgttg gagataaag aaaaagatc 
      caactttcc gagggtcaa agaaaggca gcatttgaa 
      gactgtgaa gctgaacga aaatcaaac actgacgag 
      attcacaag ctcaagact gaaatcaga gaattggtg 
      aaaaaattg aaagaaaaa acccaatca actgctgct 
      ctcatcaaa acttacaag aaactcgaa tcattactt 
      ggaccaatc gatgacaaa agcacgtcc acaattgaa 
      caattgcta gatttgcaa attatcgac aaatccaaa 
      tgcttaaac ttggtcaat tacaaaatt aaaaaacgc 
      cagaaatta ctaacacaa ttaggcgaa gaattccaa 
      ttgttacaa tcggagaaa tctctgcaa caagtcgtt 
      gaaaaagtc gataaaccg gcgaaaaaa gccacatgt 
      gaacttcag aacagtatc cacgctgtt gaggtccaa 
      ctgagggag gcaaaccac atttccgcc cgttataaa 
      gacgtgaaa aagtcactg ttacgcgac tcggcccgt 
      tttgagagc aatatcaag cgaattgag gaggaatta 
      gcgttgcaa aataacgac attaccgaa ctgcaacac 
      gtaatgaac gaagccacg aaaaaacgg agtacagca 
      cgtggacac ctccttcga gaggagaaa attgcaatg 
      tcaagtgca cattttaga gatagacaa actgtggaa 
      ggccaaaga ttggttacg aggcgaaaa caagagtta 
      gagttgctg gaaaaacgt attttccaa gttgtgaaa 
      gtcgcaaga gttgaggaa ataattgag gaggaaaat 
      gaaacaatg ctcacaact ttatcacca ccggaggaa 
      gttttaact cgggctttc gaggttttg aagaaagcg 
      acaggggcc actaacacc tcggaggtt ttacacaaa 
      tttaacatg caaaaagac accttagaa cggcttatt 
      ttgctacgg aaaaaatca gaaagcgag aagaaacaa 
      ctcgagaag aatttggac gatttaaac acgcaattg 
      gagtttttc aagtacgct gaagtgaag gaaactgaa 
      cgcaagtcg cttaaaatg gaacaaatg ctaagccaa 
      atcgacgaa atcaaaaac atatcactc aaaaccaac 
      aatttgaaa ctggtgaag gataagacg attcagacg 
      attctccga tctgttcaa gagttacaa gcttgcatt 
      aacccgatt tcggtgcca gaaagcaac gttttgggc 
      actttggag tgcattaag tccgatttg gccaaaatt 
      atccgcatt gaaaacgaa gaaaacgtc gttaaactt 
      gaagatatt gaagaaaag tggttgccg ggaccgtac 
      agtggccta gtacgtaga actccaatt ccccagccc 
      gggacttcc ccggctcct cctccacct cccacggga 
      tctgaagac gaggaggag gtcccgtcc aggacttac 
      ttgaaacgt caagcccaa ttagtcgtc gatgcgaag 
      tcacggcgc aaaaatatt cgaattccc gcaaaaaaa 
      taa
    mRNA sequence
    • >TC004392 RA
      agtttgatc aataaatag tgttttatt taaaatgaa 
      gccgaatca ggatgattt aaccccagc cgagatcaa 
      taaacaaat gttggagat aaagaaaaa gatccaact 
      ttccgaggg tcaaagaaa ggcagcatt tgaagactg 
      tgaagctga acgaaaatc aaacactga cgagattca 
      caagctcaa gactgaaat cagagaatt ggtgaaaaa 
      attgaaaga aaaaaccca atcaactgc tgctctcat 
      caaaactta caagaaact cgaatcatt acttggacc 
      aatcgatga caaaagcac gtccacaat tgaacaatt 
      gctagattt gcaaattat cgacaaatc caaatgctt 
      aaacttggt caattacaa aattaaaaa acgccagaa 
      attactaac acaattagg cgaagaatt ccaattgtt 
      acaatcgga gaaatctct gcaacaagt cgttgaaaa 
      agtcgataa accggcgaa aaaagccac atgtgaact 
      tcagaacag tatccacgc tgttgaggt ccaactgag 
      ggaggcaaa ccacatttc cgcccgtta taaagacgt 
      gaaaaagtc actgttacg cgactcggc ccgttttga 
      gagcaatat caagcgaat tgaggagga attagcgtt 
      gcaaaataa cgacattac cgaactgca acacgtaat 
      gaacgaagc cacgaaaaa acggagtac agcacgtgg 
      acacctcct tcgagagga gaaaattgc aatgtcaag 
      tgcacattt tagagatag acaaactgt ggaaggcca 
      aagattggt tacgaggcg aaaacaaga gttagagtt 
      gctggaaaa acgtatttt ccaagttgt gaaagtcgc 
      aagagttga ggaaataat tgaggagga aaatgaaac 
      aatgctcac aactttatc accaccgga ggaagtttt 
      aactcgggc tttcgaggt tttgaagaa agcgacagg 
      ggccactaa cacctcgga ggttttaca caaatttaa 
      catgcaaaa agacacctt agaacggct tattttgct 
      acggaaaaa atcagaaag cgagaagaa acaactcga 
      gaagaattt ggacgattt aaacacgca attggagtt 
      tttcaagta cgctgaagt gaaggaaac tgaacgcaa 
      gtcgcttaa aatggaaca aatgctaag ccaaatcga 
      cgaaatcaa aaacatatc actcaaaac caacaattt 
      gaaactggt gaaggataa gacgattca gacgattct 
      ccgatctgt tcaagagtt acaagcttg cattaaccc 
      gatttcggt gccagaaag caacgtttt gggcacttt 
      ggagtgcat taagtccga tttggccaa aattatccg 
      cattgaaaa cgaagaaaa cgtcgttaa acttgaaga 
      tattgaaga aaagtggtt gccgggacc gtacagtgg 
      cctagtacg tagaactcc aattcccca gcccgggac 
      ttccccggc tcctcctcc acctcccac gggatctga 
      agacgagga ggaggtccc gtccaggac ttacttgaa 
      acgtcaagc ccaattagt cgtcgatgc gaagtcacg 
      gcgcaaaaa tattcgaat tcccgcaaa aaaataaag 
      gaagtaaaa ttttttgtg taataaatt tgcaaataa 
      cta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY