Status
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Gene information
General information
TC032717
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007736
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 392649..395317
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032717
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09826

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032717 RA
      MLKQFFTLF LVSLNTINA QSIPDFLNF AQYYEDVLE 
      FLSKLEPNE CNTQLLRLF SSLQNETWA RDMLDASAK 
      PQAGIFMGN IMHVGNYDE CLRIPGKYC SALFQAHPE 
      FAPKYLKDY FSKFKAETA REDQEEELY IQGRFSLGL 
      CVPRVCRVN DLQNLSNVF ENRLKFPLH FTFVEDYCD 
      DGEAKPITA GAVVAIVIF SLFGVIVVA STLYDFFVY 
      QYVTDKKPN IHVAFSMLT NGKKLLSTK TAEGNLNCL 
      NGLRVISLM WIVLGHGFQ YSFYVPVIN SLDMEEWKN 
      FKANCLIIA CSVAVDTFF VISGLLLVY LFLKSEAKG 
      AKFSIPMFY LHRILRLTP SLAMAVLFT ATLINYMGK 
      GPFWPIVHQ MFQKDCQNY WWATLLYIQ NYAYPHNQC 
      VGQSWYLAV DTQMYFLSP LILFPLVRW PKKTLTAIG 
      VFVVLNCSY VFEITWKKQ LGTTLFDQE DQHYELLYS 
      PTHVRGVPY LIGMACGYL VFQYKTCKN LKLSKTKIL 
      FLWSLALGT LVAIVIIHY PMTQGNKHT DLFQTSFFN 
      SLHRVVWSL ALCVLIILC INGYGGLIN SFLSHKIFT 
      VLIRLNYNI YLLHMALLT IMTAQKRTP GYFSDLFCF 
      HQFWGDYMF SVATAILWT LVFESPILM IEKIIFAPK 
      KTPTPVKEQ AIDIENGQQ KNN
    CDS sequence
    • >TC032717 RA
      atgttgaaa cagtttttc acacttttt ttggtgtct 
      ctgaacaca attaatgcc caatctatc ccagatttt 
      ttaaatttc gctcaatat tatgaggac gtgttagaa 
      tttttatca aaacttgag cccaatgag tgcaatacg 
      cagcttttg cgcctcttt tccagcctt cagaacgaa 
      acgtgggcc cgcgacatg cttgatgcg agcgcaaag 
      ccccaagct ggcattttc atgggcaat ataatgcac 
      gtgggcaat tacgacgag tgtttgcga attccgggg 
      aaatactgc tcggctttg ttccaagcc catcccgaa 
      ttcgcgcca aaatactta aaagactat ttttcaaaa 
      ttcaaagcc gaaacggca agggaagat caagaagaa 
      gagttgtac atccaaggc cggttttcg ctaggtttg 
      tgcgttcct agagtttgc cgtgtgaac gatttgcaa 
      aatttgagc aacgttttt gaaaatcgg ctaaaattt 
      ccgctgcat tttacattt gttgaggat tattgtgac 
      gatggggag gctaaacca atcacagcg ggagcagtt 
      gtggcaatt gtgattttt tcactcttc ggtgtgatt 
      gttgtggcg agtactttg tacgatttt ttcgtctat 
      cagtatgtt acggacaag aagccaaac attcacgtt 
      gcgttttca atgctaacg aatggtaaa aaattattg 
      tcgaccaaa acggccgaa ggcaacttg aattgtttg 
      aacggtttg cgcgtcatt agtttgatg tggattgtg 
      ttgggacat gggttccaa tactcgttt tacgtccct 
      gtaatcaac tcactggac atggaagag tggaaaaat 
      ttcaaagct aattgtttg attatcgca tgttcggtc 
      gcagtggac acgtttttt gtcatcagt ggcttgttg 
      ctcgtttat ctttttctg aaaagtgag gcaaaaggg 
      gccaaattc tcaataccc atgttttac ttgcaccga 
      atcttacga ttaacacca tctctggcc atggcagtg 
      ttatttacc gcaactttg atcaattac atgggcaaa 
      gggccgttt tggccaata gttcaccaa atgttccag 
      aaagattgc caaaattat tggtgggcc actttgctc 
      tacatccaa aattacgcc taccctcat aaccagtgt 
      gttggacag tcttggtac ctagctgtg gacacccaa 
      atgtatttt ctttcgccc ctaatttta tttccttta 
      gttcggtgg cccaaaaaa accctaact gcgattggc 
      gttttcgtc gttttaaac tgcagttat gtctttgaa 
      attacgtgg aaaaaacag ctcgggacg acacttttc 
      gatcaagaa gatcaacac tatgaattg ctttatagc 
      cccacacat gtaaggggt gtgccgtat ttaatcggc 
      atggcttgt ggctatttg gtttttcaa tacaagact 
      tgcaaaaat ttgaaattg tcgaagaca aaaattttg 
      tttttgtgg tcattggct ttaggcaca cttgtggca 
      atagtcata attcattat ccgatgact caaggcaat 
      aaacacacc gatcttttc caaacttct ttcttcaat 
      tctttacac cgtgttgtt tggtcgctc gccttatgc 
      gttttaatc atcctttgt ataaacgga tacggcggc 
      ttaattaat tccttcctt tcgcataaa attttcacg 
      gttttgatt cgtttaaat tacaatatt tatcttcta 
      catatggct ctactaaca ataatgacc gctcagaaa 
      cgaactcct ggttatttc tccgacttg ttttgcttt 
      caccaattt tggggggat tacatgttt agtgtagct 
      acagcgatt ttgtggact ttggtattt gaatcccca 
      attctgatg attgagaaa attattttc gccccaaaa 
      aaaacgccc acaccagtc aaggaacaa gcaatagat 
      attgagaat gggcaacaa aaaaataat tag
    mRNA sequence
    • >TC032717 RA
      ttttattta tttattcca acttttatt gcacactat 
      ttgtgaagg aattacact gattgttaa gataattaa 
      tttctttgt aaaattaag cacagtttt atcacgatg 
      ttgaaacag tttttcaca ctttttttg gtgtctctg 
      aacacaatt aatgcccaa tctatccca gatttttta 
      aatttcgct caatattat gaggacgtg ttagaattt 
      ttatcaaaa cttgagccc aatgagtgc aatacgcag 
      cttttgcgc ctcttttcc agccttcag aacgaaacg 
      tgggcccgc gacatgctt gatgcgagc gcaaagccc 
      caagctggc attttcatg ggcaatata atgcacgtg 
      ggcaattac gacgagtgt ttgcgaatt ccggggaaa 
      tactgctcg gctttgttc caagcccat cccgaattc 
      gcgccaaaa tacttaaaa gactatttt tcaaaattc 
      aaagccgaa acggcaagg gaagatcaa gaagaagag 
      ttgtacatc caaggccgg ttttcgcta ggtttgtgc 
      gttcctaga gtttgccgt gtgaacgat ttgcaaaat 
      ttgagcaac gtttttgaa aatcggcta aaatttccg 
      ctgcatttt acatttgtt gaggattat tgtgacgat 
      ggggaggct aaaccaatc acagcggga gcagttgtg 
      gcaattgtg attttttca ctcttcggt gtgattgtt 
      gtggcgagt actttgtac gattttttc gtctatcag 
      tatgttacg gacaagaag ccaaacatt cacgttgcg 
      ttttcaatg ctaacgaat ggtaaaaaa ttattgtcg 
      accaaaacg gccgaaggc aacttgaat tgtttgaac 
      ggtttgcgc gtcattagt ttgatgtgg attgtgttg 
      ggacatggg ttccaatac tcgttttac gtccctgta 
      atcaactca ctggacatg gaagagtgg aaaaatttc 
      aaagctaat tgtttgatt atcgcatgt tcggtcgca 
      gtggacacg ttttttgtc atcagtggc ttgttgctc 
      gtttatctt tttctgaaa agtgaggca aaaggggcc 
      aaattctca atacccatg ttttacttg caccgaatc 
      ttacgatta acaccatct ctggccatg gcagtgtta 
      tttaccgca actttgatc aattacatg ggcaaaggg 
      ccgttttgg ccaatagtt caccaaatg ttccagaaa 
      gattgccaa aattattgg tgggccact ttgctctac 
      atccaaaat tacgcctac cctcataac cagtgtgtt 
      ggacagtct tggtaccta gctgtggac acccaaatg 
      tattttctt tcgccccta attttattt cctttagtt 
      cggtggccc aaaaaaacc ctaactgcg attggcgtt 
      ttcgtcgtt ttaaactgc agttatgtc tttgaaatt 
      acgtggaaa aaacagctc gggacgaca cttttcgat 
      caagaagat caacactat gaattgctt tatagcccc 
      acacatgta aggggtgtg ccgtattta atcggcatg 
      gcttgtggc tatttggtt tttcaatac aagacttgc 
      aaaaatttg aaattgtcg aagacaaaa attttgttt 
      ttgtggtca ttggcttta ggcacactt gtggcaata 
      gtcataatt cattatccg atgactcaa ggcaataaa 
      cacaccgat cttttccaa acttctttc ttcaattct 
      ttacaccgt gttgtttgg tcgctcgcc ttatgcgtt 
      ttaatcatc ctttgtata aacggatac ggcggctta 
      attaattcc ttcctttcg cataaaatt ttcacggtt 
      ttgattcgt ttaaattac aatatttat cttctacat 
      atggctcta ctaacaata atgaccgct cagaaacga 
      actcctggt tatttctcc gacttgttt tgctttcac 
      caattttgg ggggattac atgtttagt gtagctaca 
      gcgattttg tggactttg gtatttgaa tccccaatt 
      ctgatgatt gagaaaatt attttcgcc ccaaaaaaa 
      acgcccaca ccagtcaag gaacaagca atagatatt 
      gagaatggg caacaaaaa aataattag ttcaagtac 
      aaaaaatta ttactaaga ttatgtaac caacaatac 
      aatttgata agttgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY