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Gene information
General information
TC004235 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451620.1: 121890..125559
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004235
    iBeetle screen iB_09821

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004235 RA
      MADEKNVEK TISEDLVVT KYKMAGEIV NRILKQVLE 
      KCKPGSSVR EICEFGDQL ITEETSKVF KKEKELKKG 
      IAFPTCVSV NNCICHFSP IASESDYVL KDGDMVKVD 
      LGAHIDGFI AVVAHTIIL GVSSEHKAT DRKADVMLA 
      AHYASEAAL RLLKPGNET YTITDAVQR VAESFKCKP 
      VEGMLSHQL KQFKIDGEK TIIQNPNEA QKKEHEKFE 
      LDKHEVYAM DVLISTGEG VGREGDNRV SIYKKTDET 
      YQLKLKASR MFYSEVRTK HGNMPFNLR SFQDETKAK 
      MGVVECVKN KLIEPFQVL YEKPGEFVA HFKFTVLLM 
      PNGPHKITG LPFDPELYK SEHSISDPQ VQSILKSSA 
      NPKAAKKKK RKTEGEVDQ PMEVEAVA
    CDS sequence
    • >TC004235 RA
      atggcggac gagaaaaat gtggagaaa acaatttcg 
      gaagacttg gtcgttacc aagtataaa atggccgga 
      gaaattgtt aacagaatt ttgaaacaa gttctcgaa 
      aaatgtaag ccaggcagc tcagttcgc gagatttgc 
      gagttcgga gaccagctt atcacggaa gaaacgagc 
      aaagttttc aagaaggag aaggagcta aagaaggga 
      attgccttc ccgacgtgt gtctcggtc aacaactgt 
      atttgccat ttttctccc attgccagt gagtctgat 
      tatgtcctg aaggacggg gatatggtc aaagttgat 
      ttgggcgcg catatcgac ggtttcatc gccgtggtg 
      gcccacacg ataatccta ggtgtctca agcgagcac 
      aaggccacc gatcgaaaa gccgacgta atgctcgca 
      gcacattat gcttccgaa gccgctcta agactacta 
      aagcccggc aacgaaaca tacacaatc actgacgcc 
      gttcaaaga gtggccgaa tcgttcaaa tgtaagcca 
      gtcgagggc atgttaagc caccaactg aaacagttc 
      aaaatcgac ggcgagaaa accatcatt caaaacccg 
      aacgaagcg cagaagaaa gagcacgaa aagttcgaa 
      ctggacaag catgaggtg tatgccatg gacgtgctg 
      atcagcacc ggcgaaggc gtgggcaga gagggcgat 
      aatcgtgtc tcgatttac aaaaaaacc gacgaaact 
      tatcagtta aaactcaaa gcttcgagg atgttttac 
      agcgaagtg cgcaccaag cacgggaat atgcccttc 
      aacttgagg agttttcag gacgagacc aaggccaaa 
      atgggggtg gtggagtgc gtcaaaaat aagttaata 
      gaaccgttc caagtgctt tacgaaaaa ccgggcgaa 
      tttgtggcc catttcaag ttcacggtg ttgttgatg 
      ccgaacggc ccccacaaa atcacaggg ctgcctttc 
      gaccccgaa ctgtacaaa tcggagcac tccatcagc 
      gatccacaa gtccagtcg atactcaag agttctgct 
      aaccctaaa gctgccaaa aagaaaaaa aggaagacg 
      gaaggtgaa gtggaccaa ccaatggaa gtcgaagct 
      gtagcttaa
    mRNA sequence
    • >TC004235 RA
      aaatcgtgc atcacaccg tagtcataa gtgattccc 
      cggtcattt aacccccgt caaagttac aataaacgt 
      tttgtggaa gtataaatt caaaactgg cgcgtcagg 
      tgtgcaacc acatgacgt ttcctctct agttctcgt 
      agtgcgaga aatggcgga cgagaaaaa tgtggagaa 
      aacaatttc ggaagactt ggtcgttac caagtataa 
      aatggccgg agaaattgt taacagaat tttgaaaca 
      agttctcga aaaatgtaa gccaggcag ctcagttcg 
      cgagatttg cgagttcgg agaccagct tatcacgga 
      agaaacgag caaagtttt caagaagga gaaggagct 
      aaagaaggg aattgcctt cccgacgtg tgtctcggt 
      caacaactg tatttgcca tttttctcc cattgccag 
      tgagtctga ttatgtcct gaaggacgg ggatatggt 
      caaagttga tttgggcgc gcatatcga cggtttcat 
      cgccgtggt ggcccacac gataatcct aggtgtctc 
      aagcgagca caaggccac cgatcgaaa agccgacgt 
      aatgctcgc agcacatta tgcttccga agccgctct 
      aagactact aaagcccgg caacgaaac atacacaat 
      cactgacgc cgttcaaag agtggccga atcgttcaa 
      atgtaagcc agtcgaggg catgttaag ccaccaact 
      gaaacagtt caaaatcga cggcgagaa aaccatcat 
      tcaaaaccc gaacgaagc gcagaagaa agagcacga 
      aaagttcga actggacaa gcatgaggt gtatgccat 
      ggacgtgct gatcagcac cggcgaagg cgtgggcag 
      agagggcga taatcgtgt ctcgattta caaaaaaac 
      cgacgaaac ttatcagtt aaaactcaa agcttcgag 
      gatgtttta cagcgaagt gcgcaccaa gcacgggaa 
      tatgccctt caacttgag gagttttca ggacgagac 
      caaggccaa aatgggggt ggtggagtg cgtcaaaaa 
      taagttaat agaaccgtt ccaagtgct ttacgaaaa 
      accgggcga atttgtggc ccatttcaa gttcacggt 
      gttgttgat gccgaacgg cccccacaa aatcacagg 
      gctgccttt cgaccccga actgtacaa atcggagca 
      ctccatcag cgatccaca agtccagtc gatactcaa 
      gagttctgc taaccctaa agctgccaa aaagaaaaa 
      aaggaagac ggaaggtga agtggacca accaatgga 
      agtcgaagc tgtagctta aaaacaaat actagtttt 
      atacaagct ttctcctca cactcggca ctttgtatt 
      attttatta tctaagttt tgtatttca tttcttttt 
      taaattttc atgacgaga ttttttctt caataaaac 
      tgaccccaa gtgttgaaa ttgttaact ttaatagat 
      tttatattg ctgtgacag caatatgat gaatgaata 
      aatatagtg tttggtagt tgggcttta atttcaact 
      gcacgtgta attaaaaat gtttacacc ttttcagta 
      attgataat cctaatcga agaaaaaag acaaattaa 
      aaaatgatg atagaacat tatcttttc ctttttgga 
      gtgtagcct cgacgtttc gatcgtcct cttgaactg 
      cttcgcctc ttgtccttc ctgcgggaa cgcgatcgg 
      gacctgcgg cgtctcttc gactgccta aaaacagga 
      aaaaaggtg ttccgaaaa tatatttgc gaataaatt 
      ctactaaga ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY