| General information | |||
| TC002423 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 28749848..28777810 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002423 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09816 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002423 RA
MQSNSKTKE MFILRALEK ILNDKEIKR SHHSQLKRA CEVALEDIK KEVKDETPD GEKVSDALP MPKSGEVNV LTTEKYFLP FELACQSRS ARIVVTALD CLQKLIAYG HLTANVPDS TTPGKLLID RIVETICAC FTGPQTDEG VQLQIIKAL LTVVTSQHV EVHEGTVLL AVRTCYNIF LASKNLINQ TTAIATLTQ MLNVIFTRM ENQALDAEV QSEIQLNSS CNGIDTKSV KSLKIEDSV EGKEEIDPG SVEVVNEIL DGIISDVIT EVEHKKIAE QINEGSLSS IHRVPSQES MDTHSENDS AVTAKFTHV LQKDAFLVF RALCKLSMK PLPEGTPDP KSHELRSKI LSLQLLLSI LQNAGPVFR SNEMFITAI KQYLCVALS KNGVSSIPK VFELSLAIF LALLSNFKM HLKMQIEVF FKEIFLNIL ETTNSSFEH KWMVIQALT RICGDAQCV VDIYVNYDC DLAAANLFE RLVNDLFKV AQGRHSVEL GASPNQERA MKMLGLECL VSILKCMVE WSKDLYVNP NLQSTVGEA PINNNGSDN ASLKSHGGS TTSLHSSDC SSGGNKEIL DSPEQLEVL KQQKEVWET GIELFNRKP RKGVAFLQE HELLGTTHQ EVAKFLHNE DRLDKTFIG DFLGDNDDF CKEVMYTYV DQMDFGNME FVAALRYFL EGFRLPGEA QKIDRLMEK FASRYCECN PNNGLFASA DTAYVLGFS IIMLTTDLH SPQVKNKMS KEEYIKINR GNTDSKDVP EEYLSQIYD EIAGHEIKM KNTVNKPGK HQINSEKRR KILFNMEME AIATAAKNL MESVSHVQA PFTLAKHLD HVRPMFKTS WTSFLAAFS VGLQDCDDP EVATLCLDG IRCAIRVAC IFHMSLERD AYVQALARF TLLTTNSPI MDMKAKNID TIKTLIMVA HTDGNYLGS SWLDILKCI SQLELAQLI GTGVRPEFL SHKPPDSTS KEHIGQTSS QSVVVAVDR IFTGSTRLD GDAIVDFVK ALCQVSLEE LAYVGHPRM FSLQKIVEI SYYNMGRIR LQWSRIWQV LGEHFNTVG CNTNEEICF FAVDSLRQL SMKFIEKGE FPNFRFQKD FLRPFEHIM KKNVSPTIR DMVVRCVAQ MVNSQASNI KSGWKNIFS VFHLAASDQ EESIVELAF QTTGKIITE LYDKQFASM IDSFQDAVK CLSEFACNA RFLDTSMEA IRLVRSCAN SVSSCAHLF AEHAGMEND VTVAEEDRI WVRGWFPLL FSLSCVVNR CKLDVRTRA LTVLFEIIK TYGDTFASH WWKDLFKIL FRIFDNMKL PEQHTEKAE WMTTTCNHA LYAIVDVFT QYFDVLGPL LLDELYSQL HWCVLQDNE QLAKSGTNC LENLVNSNG HKFDEVTWD RTCRCMLDI FDSTVPTAL LTWKPEWMK TTAVIEQNG DVKGILKKP PTYDRTNSD ATLFNKLAI KSAVQLELI QTIDNIVFY PATSRKEDA ETLALAAAD MAGNGSLTE CQREEQGMY RVLTSPHLL QLTDCLLQS HRFAKAFNL DHEQRNLLW KAGYRGSTK PNLMKQETH SLACSLRIL FKMYCDESR RQHWSLIET KLIGVCQEA LDYFLKLQS EAHREEWTM LLLLVLTRL LKMPDDRFA VHVSRYYPL LCEIVCFDL KAELRSILR RVFLRIGPV FHITTAQ
- >TC002423 RA
atgcaatcg aactctaaa accaaagaa atgttcatc ttgcgagcg ctggaaaag atcctcaat gacaaggaa atcaagagg tcacaccat tcgcagctg aagcgggcc tgcgaggtg gctcttgag gacatcaag aaggaagtg aaggatgaa actccagac ggagagaaa gtttccgac gctctcccg atgccaaaa agtggcgaa gtcaacgtc ctcaccacc gaaaaatac ttcctgcct tttgaactt gcatgccag agcagatcg gcccggatt gtcgttacg gccctggat tgtttgcaa aaactgatc gcctacgga cacctcaca gccaacgtc ccggactca accactccc ggaaagctc ctgattgac cgaattgtc gaaacgatc tgtgcctgt tttacgggg ccccagacc gacgagggg gtgcagttg cagataatc aaggcgctg ctgactgtc gtcacgagc caacatgtg gaggtccat gaagggacc gttcttttg gctgttcgg acgtgttac aatattttc ctagcgagt aaaaatctc atcaatcag accacagca attgccact ttgacacaa atgcttaat gttattttt actcgaatg gagaatcaa gcactcgat gctgaagta caatctgaa attcaattg aacagttct tgtaatggg attgatacg aagtcggtc aagtcgttg aaaattgaa gacagtgtg gagggtaag gaggaaatt gatcccggg agtgtggaa gtggtcaat gaaattctg gacgggatt atcagtgat gttatcacg gaagtggag cataaaaaa attgcggag cagattaac gaaggcagt ctgtcgtca atccataga gtcccctcg caagaaagc atggacacc cacagcgag aacgactcc gccgtgacc gccaaattc acgcacgtc ctccagaag gacgctttc ctggtattc cgcgccctc tgcaagctc tctatgaaa ccgctcccc gaaggcacc cccgacccc aaatcgcac gaactccgt tcaaaaatc ctctccctc caactcctc ctttcaatc ctccaaaac gccggtccc gtattccgc tccaacgaa atgttcata accgccatc aagcaatac ctctgtgtt gccctttca aaaaacggc gtcagctcc attcccaaa gtattcgaa ctgtccttg gccatcttc ctcgccttg ctctccaac ttcaaaatg cacctcaag atgcaaatc gaggtcttc ttcaaggag attttcctc aacattctg gaaaccacc aactcttcg ttcgagcac aaatggatg gtcatccaa gccctgacc cggatctgt ggcgatgcc cagtgtgtt gtggatatc tatgtcaac tacgactgt gatttggcc gcggccaac ttattcgaa cgtctagtc aacgatttg ttcaaagtc gcgcaaggg cggcattcg gtggaattg ggggcttcc cccaaccaa gagcgcgcg atgaaaatg ctcggcttg gagtgtctt gtctcgatt ttgaaatgc atggttgaa tggagcaag gatttgtac gtcaacccc aatctccag tcaactgta ggggaagcc ccgattaat aacaacgga agcgacaac gcctcccta aaatcacac ggcggttcc accacaagt ctgcattca agcgactgt tcaagtggc ggtaataag gaaatcctc gactcgcct gaacaactc gaagtcctc aaacaacaa aaggaagta tgggagacc ggaatcgag ttgttcaac cgcaagcca cgcaaaggc gtcgccttc ctgcaagag cacgaactt ttaggcaca acccaccaa gaagtggcc aagtttttg cacaacgaa gaccgtcta gacaagact ttcattggc gattttttg ggcgacaat gacgatttt tgcaaggaa gtcatgtac acgtatgtc gatcaaatg gacttcggg aacatggaa tttgtggct gcgcttcgc tactttctg gaaggcttc cgcttgccg ggcgaagcc cagaaaatc gaccgtctg atggagaag tttgccagt cgctactgt gagtgtaac cccaataat gggcttttt gcaagcgct gacaccgct tacgtgctg ggcttctcc attattatg ctaacaact gacttgcac tccccccaa gttaagaac aagatgagc aaggaggaa tatatcaaa atcaatcgg gggaatacc gacagtaag gacgtacca gaggagtac ttgtcgcag atttatgac gaaatcgcc gggcatgaa atcaaaatg aaaaatacg gtcaataag ccggggaaa caccaaatc aatagtgaa aaacgccgc aagattttg ttcaacatg gaaatggaa gcgattgcc acggctgcc aaaaacctg atggagtct gtctcgcac gtgcaagcc cccttcaca ctggccaaa cacttggac cacgtgcgc ccaatgttc aagacttcc tggacgtcc tttctagcc gccttcagt gtggggttg caagattgt gacgatcct gaagttgca actttgtgt cttgatggc atccggtgt gccatcaga gtggcttgt attttccac atgtcacta gagcgtgac gcctatgtc caggctttg gcccgcttt acactcttg accacaaac tcgccaatt atggacatg aaggccaaa aacatcgac acgattaaa accctaatc atggtcgca cacaccgat ggcaattat ctcggatcg agttggctt gatattttg aaatgtatc tcgcaactg gagctcgcc cagttgatc ggcaccgga gttagaccg gagtttctc tcgcataag ccgccggat tcgacgtcg aaggagcac atcggacag acgagttcg cagagtgtt gtcgtggcc gttgaccgg attttcacc ggatcgacg aggcttgat ggcgacgcg atcgtcgat tttgtgaag gcgctgtgt caggtgtcg ttggaggag ttggcctat gtggggcat ccccggatg ttttcgttg cagaaaatt gtggagatt tcctattat aatatgggg aggatcagg ttgcagtgg tcgaggatt tggcaggtt ttgggggag cattttaat acagtgggc tgtaatacc aacgaggag atttgtttc tttgcggtg gatagttta agacagctg tccatgaag tttattgag aagggggag tttccgaat tttcgcttc cagaaggat tttctaagg ccgtttgag catatcatg aagaagaat gtgtcgccg acgatcagg gatatggtg gttcgttgt gtggcccag atggtgaac tcccaggcc agcaacata aaatccggc tggaagaac atcttctcg gtgttccac ttggccgcc agcgaccag gaggagtca atcgtcgag ctcgctttc caaacaacc ggcaaaatc atcaccgaa ctctacgac aagcagttc gcctccatg atcgactcg ttccaagac gccgtcaag tgcctctcc gaattcgct tgcaacgcc cgtttcctg gacacctcc atggaggcg atccgcctg gtgcgctcc tgcgccaac agcgtcagc tcctgcgcc cacctcttc gccgagcac gccggcatg gagaacgac gtcaccgtc gccgaggag gaccgcatc tgggtgcgg ggctggttc ccgctgctc ttctccctc tcgtgtgtg gtgaatcgg tgcaagttg gacgtgcgg acgcgcgct ttgaccgtt cttttcgaa ataatcaaa acctacggc gacacgttc gcgagtcac tggtggaag gatttgttt aagattttg ttccggatt ttcgataat atgaagctg cccgaacag catacggag aaggccgag tggatgacg accacttgt aatcatgcc ttgtacgcg attgttgac gttttcacg cagtatttt gacgttttg ggaccgctg ttgttagat gagttgtac tcgcagttg cattggtgt gtgttacag gataatgaa cagttggcc aagtcgggg accaattgt ttggagaat ttggttaat tcaaatgga cataagttt gatgaggtc acgtgggac cgcacgtgc cgttgcatg ctggacatt ttcgactcg acagtcccc acagccctc ttgacctgg aagcccgaa tggatgaag acaacagcc gtaatcgaa caaaacggc gatgttaaa ggcattctt aaaaaacca ccaacctac gaccgaacc aattcggac gccactctg ttcaataaa ctggcaatc aagagtgcg gtccagctg gaactgatt caaaccatt gacaacatc gtcttctat ccagcaact tcacggaaa gaagacgcc gaaactttg gcactagct gcagccgac atggccgga aacggttcc ttgaccgag tgccaaagg gaggaacag gggatgtac cgagttttg acatcaccg catctttta cagttgact gattgtctc ctccagtcg cacagattt gccaaagcg tttaatctc gaccacgag cagaggaat ttgctgtgg aaggcgggg tatcgaggt tcgactaaa ccgaatttg atgaaacag gaaacgcat agtttggct tgttcgtta aggattctg ttcaaaatg tattgtgat gagagtcga aggcagcac tggtcgttg attgagacg aaattgatt ggagtgtgt caggaggcg ttggattat tttttgaag ttgcaaagt gaggcacat agggaggag tggaccatg ttgttgttg ttggtgttg acgagactg cttaaaatg ccagacgat aggttcgcc gtgcacgtg tcgagatat taccccctt ctgtgcgaa atcgtctgt ttcgatctc aaagccgag ctcaggtcc atcctgcga cgagttttc ctgaggata ggaccggtg tttcacatc acgacggca cagtga
- >TC002423 RA
ttacgatcg taatttgac aggtgtgtg acatgtcca gttacggat aaacataaa agtgtttaa aaaagcaca ttactgaaa attgccaaa gttaacagt gttataaag cgaatgttt acgtgtaga gactcgaat cggcacatc tgtcacgca aaacccttc attagtgca aatgcgtac aaagaatcg ttcaatatc tgttagttc aacacgtca caagtcatg atccctctt ttcaagggt tttaactga ttcaaccac cagtagatt aatgatgca atcgaactc taaaaccaa agaaatgtt catcttgcg agcgctgga aaagatcct caatgacaa ggaaatcaa gaggtcaca ccattcgca gctgaagcg ggcctgcga ggtggctct tgaggacat caagaagga agtgaagga tgaaactcc agacggaga gaaagtttc cgacgctct cccgatgcc aaaaagtgg cgaagtcaa cgtcctcac caccgaaaa atacttcct gccttttga acttgcatg ccagagcag atcggcccg gattgtcgt tacggccct ggattgttt gcaaaaact gatcgccta cggacacct cacagccaa cgtcccgga ctcaaccac tcccggaaa gctcctgat tgaccgaat tgtcgaaac gatctgtgc ctgttttac ggggcccca gaccgacga gggggtgca gttgcagat aatcaaggc gctgctgac tgtcgtcac gagccaaca tgtggaggt ccatgaagg gaccgttct tttggctgt tcggacgtg ttacaatat tttcctagc gagtaaaaa tctcatcaa tcagaccac agcaattgc cactttgac acaaatgct taatgttat ttttactcg aatggagaa tcaagcact cgatgctga agtacaatc tgaaattca attgaacag ttcttgtaa tgggattga tacgaagtc ggtcaagtc gttgaaaat tgaagacag tgtggaggg taaggagga aattgatcc cgggagtgt ggaagtggt caatgaaat tctggacgg gattatcag tgatgttat cacggaagt ggagcataa aaaaattgc ggagcagat taacgaagg cagtctgtc gtcaatcca tagagtccc ctcgcaaga aagcatgga cacccacag cgagaacga ctccgccgt gaccgccaa attcacgca cgtcctcca gaaggacgc tttcctggt attccgcgc cctctgcaa gctctctat gaaaccgct ccccgaagg cacccccga ccccaaatc gcacgaact ccgttcaaa aatcctctc cctccaact cctcctttc aatcctcca aaacgccgg tcccgtatt ccgctccaa cgaaatgtt cataaccgc catcaagca atacctctg tgttgccct ttcaaaaaa cggcgtcag ctccattcc caaagtatt cgaactgtc cttggccat cttcctcgc cttgctctc caacttcaa aatgcacct caagatgca aatcgaggt cttcttcaa ggagatttt cctcaacat tctggaaac caccaactc ttcgttcga gcacaaatg gatggtcat ccaagccct gacccggat ctgtggcga tgcccagtg tgttgtgga tatctatgt caactacga ctgtgattt ggccgcggc caacttatt cgaacgtct agtcaacga tttgttcaa agtcgcgca agggcggca ttcggtgga attgggggc ttcccccaa ccaagagcg cgcgatgaa aatgctcgg cttggagtg tcttgtctc gattttgaa atgcatggt tgaatggag caaggattt gtacgtcaa ccccaatct ccagtcaac tgtagggga agccccgat taataacaa cggaagcga caacgcctc cctaaaatc acacggcgg ttccaccac aagtctgca ttcaagcga ctgttcaag tggcggtaa taaggaaat cctcgactc gcctgaaca actcgaagt cctcaaaca acaaaagga agtatggga gaccggaat cgagttgtt caaccgcaa gccacgcaa aggcgtcgc cttcctgca agagcacga acttttagg cacaaccca ccaagaagt ggccaagtt tttgcacaa cgaagaccg tctagacaa gactttcat tggcgattt tttgggcga caatgacga tttttgcaa ggaagtcat gtacacgta tgtcgatca aatggactt cgggaacat ggaatttgt ggctgcgct tcgctactt tctggaagg cttccgctt gccgggcga agcccagaa aatcgaccg tctgatgga gaagtttgc cagtcgcta ctgtgagtg taaccccaa taatgggct ttttgcaag cgctgacac cgcttacgt gctgggctt ctccattat tatgctaac aactgactt gcactcccc ccaagttaa gaacaagat gagcaagga ggaatatat caaaatcaa tcgggggaa taccgacag taaggacgt accagagga gtacttgtc gcagattta tgacgaaat cgccgggca tgaaatcaa aatgaaaaa tacggtcaa taagccggg gaaacacca aatcaatag tgaaaaacg ccgcaagat tttgttcaa catggaaat ggaagcgat tgccacggc tgccaaaaa cctgatgga gtctgtctc gcacgtgca agccccctt cacactggc caaacactt ggaccacgt gcgcccaat gttcaagac ttcctggac gtcctttct agccgcctt cagtgtggg gttgcaaga ttgtgacga tcctgaagt tgcaacttt gtgtcttga tggcatccg gtgtgccat cagagtggc ttgtatttt ccacatgtc actagagcg tgacgccta tgtccaggc tttggcccg ctttacact cttgaccac aaactcgcc aattatgga catgaaggc caaaaacat cgacacgat taaaaccct aatcatggt cgcacacac cgatggcaa ttatctcgg atcgagttg gcttgatat tttgaaatg tatctcgca actggagct cgcccagtt gatcggcac cggagttag accggagtt tctctcgca taagccgcc ggattcgac gtcgaagga gcacatcgg acagacgag ttcgcagag tgttgtcgt ggccgttga ccggatttt caccggatc gacgaggct tgatggcga cgcgatcgt cgattttgt gaaggcgct gtgtcaggt gtcgttgga ggagttggc ctatgtggg gcatccccg gatgttttc gttgcagaa aattgtgga gatttccta ttataatat ggggaggat caggttgca gtggtcgag gatttggca ggttttggg ggagcattt taatacagt gggctgtaa taccaacga ggagatttg tttctttgc ggtggatag tttaagaca gctgtccat gaagtttat tgagaaggg ggagtttcc gaattttcg cttccagaa ggattttct aaggccgtt tgagcatat catgaagaa gaatgtgtc gccgacgat cagggatat ggtggttcg ttgtgtggc ccagatggt gaactccca ggccagcaa cataaaatc cggctggaa gaacatctt ctcggtgtt ccacttggc cgccagcga ccaggagga gtcaatcgt cgagctcgc tttccaaac aaccggcaa aatcatcac cgaactcta cgacaagca gttcgcctc catgatcga ctcgttcca agacgccgt caagtgcct ctccgaatt cgcttgcaa cgcccgttt cctggacac ctccatgga ggcgatccg cctggtgcg ctcctgcgc caacagcgt cagctcctg cgcccacct cttcgccga gcacgccgg catggagaa cgacgtcac cgtcgccga ggaggaccg catctgggt gcggggctg gttcccgct gctcttctc cctctcgtg tgtggtgaa tcggtgcaa gttggacgt gcggacgcg cgctttgac cgttctttt cgaaataat caaaaccta cggcgacac gttcgcgag tcactggtg gaaggattt gtttaagat tttgttccg gattttcga taatatgaa gctgcccga acagcatac ggagaaggc cgagtggat gacgaccac ttgtaatca tgccttgta cgcgattgt tgacgtttt cacgcagta ttttgacgt tttgggacc gctgttgtt agatgagtt gtactcgca gttgcattg gtgtgtgtt acaggataa tgaacagtt ggccaagtc ggggaccaa ttgtttgga gaatttggt taattcaaa tggacataa gtttgatga ggtcacgtg ggaccgcac gtgccgttg catgctgga cattttcga ctcgacagt ccccacagc cctcttgac ctggaagcc cgaatggat gaagacaac agccgtaat cgaacaaaa cggcgatgt taaaggcat tcttaaaaa accaccaac ctacgaccg aaccaattc ggacgccac tctgttcaa taaactggc aatcaagag tgcggtcca gctggaact gattcaaac cattgacaa catcgtctt ctatccagc aacttcacg gaaagaaga cgccgaaac tttggcact agctgcagc cgacatggc cggaaacgg ttccttgac cgagtgcca aagggagga acaggggat gtaccgagt tttgacatc accgcatct tttacagtt gactgattg tctcctcca gtcgcacag atttgccaa agcgtttaa tctcgacca cgagcagag gaatttgct gtggaaggc ggggtatcg aggttcgac taaaccgaa tttgatgaa acaggaaac gcatagttt ggcttgttc gttaaggat tctgttcaa aatgtattg tgatgagag tcgaaggca gcactggtc gttgattga gacgaaatt gattggagt gtgtcagga ggcgttgga ttatttttt gaagttgca aagtgaggc acataggga ggagtggac catgttgtt gttgttggt gttgacgag actgcttaa aatgccaga cgataggtt cgccgtgca cgtgtcgag atattaccc ccttctgtg cgaaatcgt ctgtttcga tctcaaagc cgagctcag gtccatcct gcgacgagt tttcctgag gataggacc ggtgtttca catcacgac ggcacagtg actaaagtg attttacca tttaacctg aatttttta tcaatgtta caagtttat tatttattt aaaaatcct aaactttaa gtaaccgtt ttgtgtgta tttttatca ttctctcaa cttaatcgc ttactagga acacctctg aacgaacag tgagactca gcttacgtc gtagaaatg atcattcct tgcatgaag tgattttta ggagccaat aaatactac aaaagtata agttacgtg ccttcaatc aaattattt atgtatgat aattgtaat tagtcattc ggtatgtgg ttatgttaa tgtgtacta tcatctgtt ataaatagc aaactcgtg taaataatc atgaaatac tactttatt tagtcatat tcgctactg ttatacaat aataaaagt aaatatatc at
