Status
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Gene information
General information
TC002423 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28749848..28777810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002423
    iBeetle screen iB_09816

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002423 RA
      MQSNSKTKE MFILRALEK ILNDKEIKR SHHSQLKRA 
      CEVALEDIK KEVKDETPD GEKVSDALP MPKSGEVNV 
      LTTEKYFLP FELACQSRS ARIVVTALD CLQKLIAYG 
      HLTANVPDS TTPGKLLID RIVETICAC FTGPQTDEG 
      VQLQIIKAL LTVVTSQHV EVHEGTVLL AVRTCYNIF 
      LASKNLINQ TTAIATLTQ MLNVIFTRM ENQALDAEV 
      QSEIQLNSS CNGIDTKSV KSLKIEDSV EGKEEIDPG 
      SVEVVNEIL DGIISDVIT EVEHKKIAE QINEGSLSS 
      IHRVPSQES MDTHSENDS AVTAKFTHV LQKDAFLVF 
      RALCKLSMK PLPEGTPDP KSHELRSKI LSLQLLLSI 
      LQNAGPVFR SNEMFITAI KQYLCVALS KNGVSSIPK 
      VFELSLAIF LALLSNFKM HLKMQIEVF FKEIFLNIL 
      ETTNSSFEH KWMVIQALT RICGDAQCV VDIYVNYDC 
      DLAAANLFE RLVNDLFKV AQGRHSVEL GASPNQERA 
      MKMLGLECL VSILKCMVE WSKDLYVNP NLQSTVGEA 
      PINNNGSDN ASLKSHGGS TTSLHSSDC SSGGNKEIL 
      DSPEQLEVL KQQKEVWET GIELFNRKP RKGVAFLQE 
      HELLGTTHQ EVAKFLHNE DRLDKTFIG DFLGDNDDF 
      CKEVMYTYV DQMDFGNME FVAALRYFL EGFRLPGEA 
      QKIDRLMEK FASRYCECN PNNGLFASA DTAYVLGFS 
      IIMLTTDLH SPQVKNKMS KEEYIKINR GNTDSKDVP 
      EEYLSQIYD EIAGHEIKM KNTVNKPGK HQINSEKRR 
      KILFNMEME AIATAAKNL MESVSHVQA PFTLAKHLD 
      HVRPMFKTS WTSFLAAFS VGLQDCDDP EVATLCLDG 
      IRCAIRVAC IFHMSLERD AYVQALARF TLLTTNSPI 
      MDMKAKNID TIKTLIMVA HTDGNYLGS SWLDILKCI 
      SQLELAQLI GTGVRPEFL SHKPPDSTS KEHIGQTSS 
      QSVVVAVDR IFTGSTRLD GDAIVDFVK ALCQVSLEE 
      LAYVGHPRM FSLQKIVEI SYYNMGRIR LQWSRIWQV 
      LGEHFNTVG CNTNEEICF FAVDSLRQL SMKFIEKGE 
      FPNFRFQKD FLRPFEHIM KKNVSPTIR DMVVRCVAQ 
      MVNSQASNI KSGWKNIFS VFHLAASDQ EESIVELAF 
      QTTGKIITE LYDKQFASM IDSFQDAVK CLSEFACNA 
      RFLDTSMEA IRLVRSCAN SVSSCAHLF AEHAGMEND 
      VTVAEEDRI WVRGWFPLL FSLSCVVNR CKLDVRTRA 
      LTVLFEIIK TYGDTFASH WWKDLFKIL FRIFDNMKL 
      PEQHTEKAE WMTTTCNHA LYAIVDVFT QYFDVLGPL 
      LLDELYSQL HWCVLQDNE QLAKSGTNC LENLVNSNG 
      HKFDEVTWD RTCRCMLDI FDSTVPTAL LTWKPEWMK 
      TTAVIEQNG DVKGILKKP PTYDRTNSD ATLFNKLAI 
      KSAVQLELI QTIDNIVFY PATSRKEDA ETLALAAAD 
      MAGNGSLTE CQREEQGMY RVLTSPHLL QLTDCLLQS 
      HRFAKAFNL DHEQRNLLW KAGYRGSTK PNLMKQETH 
      SLACSLRIL FKMYCDESR RQHWSLIET KLIGVCQEA 
      LDYFLKLQS EAHREEWTM LLLLVLTRL LKMPDDRFA 
      VHVSRYYPL LCEIVCFDL KAELRSILR RVFLRIGPV 
      FHITTAQ
    CDS sequence
    • >TC002423 RA
      atgcaatcg aactctaaa accaaagaa atgttcatc 
      ttgcgagcg ctggaaaag atcctcaat gacaaggaa 
      atcaagagg tcacaccat tcgcagctg aagcgggcc 
      tgcgaggtg gctcttgag gacatcaag aaggaagtg 
      aaggatgaa actccagac ggagagaaa gtttccgac 
      gctctcccg atgccaaaa agtggcgaa gtcaacgtc 
      ctcaccacc gaaaaatac ttcctgcct tttgaactt 
      gcatgccag agcagatcg gcccggatt gtcgttacg 
      gccctggat tgtttgcaa aaactgatc gcctacgga 
      cacctcaca gccaacgtc ccggactca accactccc 
      ggaaagctc ctgattgac cgaattgtc gaaacgatc 
      tgtgcctgt tttacgggg ccccagacc gacgagggg 
      gtgcagttg cagataatc aaggcgctg ctgactgtc 
      gtcacgagc caacatgtg gaggtccat gaagggacc 
      gttcttttg gctgttcgg acgtgttac aatattttc 
      ctagcgagt aaaaatctc atcaatcag accacagca 
      attgccact ttgacacaa atgcttaat gttattttt 
      actcgaatg gagaatcaa gcactcgat gctgaagta 
      caatctgaa attcaattg aacagttct tgtaatggg 
      attgatacg aagtcggtc aagtcgttg aaaattgaa 
      gacagtgtg gagggtaag gaggaaatt gatcccggg 
      agtgtggaa gtggtcaat gaaattctg gacgggatt 
      atcagtgat gttatcacg gaagtggag cataaaaaa 
      attgcggag cagattaac gaaggcagt ctgtcgtca 
      atccataga gtcccctcg caagaaagc atggacacc 
      cacagcgag aacgactcc gccgtgacc gccaaattc 
      acgcacgtc ctccagaag gacgctttc ctggtattc 
      cgcgccctc tgcaagctc tctatgaaa ccgctcccc 
      gaaggcacc cccgacccc aaatcgcac gaactccgt 
      tcaaaaatc ctctccctc caactcctc ctttcaatc 
      ctccaaaac gccggtccc gtattccgc tccaacgaa 
      atgttcata accgccatc aagcaatac ctctgtgtt 
      gccctttca aaaaacggc gtcagctcc attcccaaa 
      gtattcgaa ctgtccttg gccatcttc ctcgccttg 
      ctctccaac ttcaaaatg cacctcaag atgcaaatc 
      gaggtcttc ttcaaggag attttcctc aacattctg 
      gaaaccacc aactcttcg ttcgagcac aaatggatg 
      gtcatccaa gccctgacc cggatctgt ggcgatgcc 
      cagtgtgtt gtggatatc tatgtcaac tacgactgt 
      gatttggcc gcggccaac ttattcgaa cgtctagtc 
      aacgatttg ttcaaagtc gcgcaaggg cggcattcg 
      gtggaattg ggggcttcc cccaaccaa gagcgcgcg 
      atgaaaatg ctcggcttg gagtgtctt gtctcgatt 
      ttgaaatgc atggttgaa tggagcaag gatttgtac 
      gtcaacccc aatctccag tcaactgta ggggaagcc 
      ccgattaat aacaacgga agcgacaac gcctcccta 
      aaatcacac ggcggttcc accacaagt ctgcattca 
      agcgactgt tcaagtggc ggtaataag gaaatcctc 
      gactcgcct gaacaactc gaagtcctc aaacaacaa 
      aaggaagta tgggagacc ggaatcgag ttgttcaac 
      cgcaagcca cgcaaaggc gtcgccttc ctgcaagag 
      cacgaactt ttaggcaca acccaccaa gaagtggcc 
      aagtttttg cacaacgaa gaccgtcta gacaagact 
      ttcattggc gattttttg ggcgacaat gacgatttt 
      tgcaaggaa gtcatgtac acgtatgtc gatcaaatg 
      gacttcggg aacatggaa tttgtggct gcgcttcgc 
      tactttctg gaaggcttc cgcttgccg ggcgaagcc 
      cagaaaatc gaccgtctg atggagaag tttgccagt 
      cgctactgt gagtgtaac cccaataat gggcttttt 
      gcaagcgct gacaccgct tacgtgctg ggcttctcc 
      attattatg ctaacaact gacttgcac tccccccaa 
      gttaagaac aagatgagc aaggaggaa tatatcaaa 
      atcaatcgg gggaatacc gacagtaag gacgtacca 
      gaggagtac ttgtcgcag atttatgac gaaatcgcc 
      gggcatgaa atcaaaatg aaaaatacg gtcaataag 
      ccggggaaa caccaaatc aatagtgaa aaacgccgc 
      aagattttg ttcaacatg gaaatggaa gcgattgcc 
      acggctgcc aaaaacctg atggagtct gtctcgcac 
      gtgcaagcc cccttcaca ctggccaaa cacttggac 
      cacgtgcgc ccaatgttc aagacttcc tggacgtcc 
      tttctagcc gccttcagt gtggggttg caagattgt 
      gacgatcct gaagttgca actttgtgt cttgatggc 
      atccggtgt gccatcaga gtggcttgt attttccac 
      atgtcacta gagcgtgac gcctatgtc caggctttg 
      gcccgcttt acactcttg accacaaac tcgccaatt 
      atggacatg aaggccaaa aacatcgac acgattaaa 
      accctaatc atggtcgca cacaccgat ggcaattat 
      ctcggatcg agttggctt gatattttg aaatgtatc 
      tcgcaactg gagctcgcc cagttgatc ggcaccgga 
      gttagaccg gagtttctc tcgcataag ccgccggat 
      tcgacgtcg aaggagcac atcggacag acgagttcg 
      cagagtgtt gtcgtggcc gttgaccgg attttcacc 
      ggatcgacg aggcttgat ggcgacgcg atcgtcgat 
      tttgtgaag gcgctgtgt caggtgtcg ttggaggag 
      ttggcctat gtggggcat ccccggatg ttttcgttg 
      cagaaaatt gtggagatt tcctattat aatatgggg 
      aggatcagg ttgcagtgg tcgaggatt tggcaggtt 
      ttgggggag cattttaat acagtgggc tgtaatacc 
      aacgaggag atttgtttc tttgcggtg gatagttta 
      agacagctg tccatgaag tttattgag aagggggag 
      tttccgaat tttcgcttc cagaaggat tttctaagg 
      ccgtttgag catatcatg aagaagaat gtgtcgccg 
      acgatcagg gatatggtg gttcgttgt gtggcccag 
      atggtgaac tcccaggcc agcaacata aaatccggc 
      tggaagaac atcttctcg gtgttccac ttggccgcc 
      agcgaccag gaggagtca atcgtcgag ctcgctttc 
      caaacaacc ggcaaaatc atcaccgaa ctctacgac 
      aagcagttc gcctccatg atcgactcg ttccaagac 
      gccgtcaag tgcctctcc gaattcgct tgcaacgcc 
      cgtttcctg gacacctcc atggaggcg atccgcctg 
      gtgcgctcc tgcgccaac agcgtcagc tcctgcgcc 
      cacctcttc gccgagcac gccggcatg gagaacgac 
      gtcaccgtc gccgaggag gaccgcatc tgggtgcgg 
      ggctggttc ccgctgctc ttctccctc tcgtgtgtg 
      gtgaatcgg tgcaagttg gacgtgcgg acgcgcgct 
      ttgaccgtt cttttcgaa ataatcaaa acctacggc 
      gacacgttc gcgagtcac tggtggaag gatttgttt 
      aagattttg ttccggatt ttcgataat atgaagctg 
      cccgaacag catacggag aaggccgag tggatgacg 
      accacttgt aatcatgcc ttgtacgcg attgttgac 
      gttttcacg cagtatttt gacgttttg ggaccgctg 
      ttgttagat gagttgtac tcgcagttg cattggtgt 
      gtgttacag gataatgaa cagttggcc aagtcgggg 
      accaattgt ttggagaat ttggttaat tcaaatgga 
      cataagttt gatgaggtc acgtgggac cgcacgtgc 
      cgttgcatg ctggacatt ttcgactcg acagtcccc 
      acagccctc ttgacctgg aagcccgaa tggatgaag 
      acaacagcc gtaatcgaa caaaacggc gatgttaaa 
      ggcattctt aaaaaacca ccaacctac gaccgaacc 
      aattcggac gccactctg ttcaataaa ctggcaatc 
      aagagtgcg gtccagctg gaactgatt caaaccatt 
      gacaacatc gtcttctat ccagcaact tcacggaaa 
      gaagacgcc gaaactttg gcactagct gcagccgac 
      atggccgga aacggttcc ttgaccgag tgccaaagg 
      gaggaacag gggatgtac cgagttttg acatcaccg 
      catctttta cagttgact gattgtctc ctccagtcg 
      cacagattt gccaaagcg tttaatctc gaccacgag 
      cagaggaat ttgctgtgg aaggcgggg tatcgaggt 
      tcgactaaa ccgaatttg atgaaacag gaaacgcat 
      agtttggct tgttcgtta aggattctg ttcaaaatg 
      tattgtgat gagagtcga aggcagcac tggtcgttg 
      attgagacg aaattgatt ggagtgtgt caggaggcg 
      ttggattat tttttgaag ttgcaaagt gaggcacat 
      agggaggag tggaccatg ttgttgttg ttggtgttg 
      acgagactg cttaaaatg ccagacgat aggttcgcc 
      gtgcacgtg tcgagatat taccccctt ctgtgcgaa 
      atcgtctgt ttcgatctc aaagccgag ctcaggtcc 
      atcctgcga cgagttttc ctgaggata ggaccggtg 
      tttcacatc acgacggca cagtga
    mRNA sequence
    • >TC002423 RA
      ttacgatcg taatttgac aggtgtgtg acatgtcca 
      gttacggat aaacataaa agtgtttaa aaaagcaca 
      ttactgaaa attgccaaa gttaacagt gttataaag 
      cgaatgttt acgtgtaga gactcgaat cggcacatc 
      tgtcacgca aaacccttc attagtgca aatgcgtac 
      aaagaatcg ttcaatatc tgttagttc aacacgtca 
      caagtcatg atccctctt ttcaagggt tttaactga 
      ttcaaccac cagtagatt aatgatgca atcgaactc 
      taaaaccaa agaaatgtt catcttgcg agcgctgga 
      aaagatcct caatgacaa ggaaatcaa gaggtcaca 
      ccattcgca gctgaagcg ggcctgcga ggtggctct 
      tgaggacat caagaagga agtgaagga tgaaactcc 
      agacggaga gaaagtttc cgacgctct cccgatgcc 
      aaaaagtgg cgaagtcaa cgtcctcac caccgaaaa 
      atacttcct gccttttga acttgcatg ccagagcag 
      atcggcccg gattgtcgt tacggccct ggattgttt 
      gcaaaaact gatcgccta cggacacct cacagccaa 
      cgtcccgga ctcaaccac tcccggaaa gctcctgat 
      tgaccgaat tgtcgaaac gatctgtgc ctgttttac 
      ggggcccca gaccgacga gggggtgca gttgcagat 
      aatcaaggc gctgctgac tgtcgtcac gagccaaca 
      tgtggaggt ccatgaagg gaccgttct tttggctgt 
      tcggacgtg ttacaatat tttcctagc gagtaaaaa 
      tctcatcaa tcagaccac agcaattgc cactttgac 
      acaaatgct taatgttat ttttactcg aatggagaa 
      tcaagcact cgatgctga agtacaatc tgaaattca 
      attgaacag ttcttgtaa tgggattga tacgaagtc 
      ggtcaagtc gttgaaaat tgaagacag tgtggaggg 
      taaggagga aattgatcc cgggagtgt ggaagtggt 
      caatgaaat tctggacgg gattatcag tgatgttat 
      cacggaagt ggagcataa aaaaattgc ggagcagat 
      taacgaagg cagtctgtc gtcaatcca tagagtccc 
      ctcgcaaga aagcatgga cacccacag cgagaacga 
      ctccgccgt gaccgccaa attcacgca cgtcctcca 
      gaaggacgc tttcctggt attccgcgc cctctgcaa 
      gctctctat gaaaccgct ccccgaagg cacccccga 
      ccccaaatc gcacgaact ccgttcaaa aatcctctc 
      cctccaact cctcctttc aatcctcca aaacgccgg 
      tcccgtatt ccgctccaa cgaaatgtt cataaccgc 
      catcaagca atacctctg tgttgccct ttcaaaaaa 
      cggcgtcag ctccattcc caaagtatt cgaactgtc 
      cttggccat cttcctcgc cttgctctc caacttcaa 
      aatgcacct caagatgca aatcgaggt cttcttcaa 
      ggagatttt cctcaacat tctggaaac caccaactc 
      ttcgttcga gcacaaatg gatggtcat ccaagccct 
      gacccggat ctgtggcga tgcccagtg tgttgtgga 
      tatctatgt caactacga ctgtgattt ggccgcggc 
      caacttatt cgaacgtct agtcaacga tttgttcaa 
      agtcgcgca agggcggca ttcggtgga attgggggc 
      ttcccccaa ccaagagcg cgcgatgaa aatgctcgg 
      cttggagtg tcttgtctc gattttgaa atgcatggt 
      tgaatggag caaggattt gtacgtcaa ccccaatct 
      ccagtcaac tgtagggga agccccgat taataacaa 
      cggaagcga caacgcctc cctaaaatc acacggcgg 
      ttccaccac aagtctgca ttcaagcga ctgttcaag 
      tggcggtaa taaggaaat cctcgactc gcctgaaca 
      actcgaagt cctcaaaca acaaaagga agtatggga 
      gaccggaat cgagttgtt caaccgcaa gccacgcaa 
      aggcgtcgc cttcctgca agagcacga acttttagg 
      cacaaccca ccaagaagt ggccaagtt tttgcacaa 
      cgaagaccg tctagacaa gactttcat tggcgattt 
      tttgggcga caatgacga tttttgcaa ggaagtcat 
      gtacacgta tgtcgatca aatggactt cgggaacat 
      ggaatttgt ggctgcgct tcgctactt tctggaagg 
      cttccgctt gccgggcga agcccagaa aatcgaccg 
      tctgatgga gaagtttgc cagtcgcta ctgtgagtg 
      taaccccaa taatgggct ttttgcaag cgctgacac 
      cgcttacgt gctgggctt ctccattat tatgctaac 
      aactgactt gcactcccc ccaagttaa gaacaagat 
      gagcaagga ggaatatat caaaatcaa tcgggggaa 
      taccgacag taaggacgt accagagga gtacttgtc 
      gcagattta tgacgaaat cgccgggca tgaaatcaa 
      aatgaaaaa tacggtcaa taagccggg gaaacacca 
      aatcaatag tgaaaaacg ccgcaagat tttgttcaa 
      catggaaat ggaagcgat tgccacggc tgccaaaaa 
      cctgatgga gtctgtctc gcacgtgca agccccctt 
      cacactggc caaacactt ggaccacgt gcgcccaat 
      gttcaagac ttcctggac gtcctttct agccgcctt 
      cagtgtggg gttgcaaga ttgtgacga tcctgaagt 
      tgcaacttt gtgtcttga tggcatccg gtgtgccat 
      cagagtggc ttgtatttt ccacatgtc actagagcg 
      tgacgccta tgtccaggc tttggcccg ctttacact 
      cttgaccac aaactcgcc aattatgga catgaaggc 
      caaaaacat cgacacgat taaaaccct aatcatggt 
      cgcacacac cgatggcaa ttatctcgg atcgagttg 
      gcttgatat tttgaaatg tatctcgca actggagct 
      cgcccagtt gatcggcac cggagttag accggagtt 
      tctctcgca taagccgcc ggattcgac gtcgaagga 
      gcacatcgg acagacgag ttcgcagag tgttgtcgt 
      ggccgttga ccggatttt caccggatc gacgaggct 
      tgatggcga cgcgatcgt cgattttgt gaaggcgct 
      gtgtcaggt gtcgttgga ggagttggc ctatgtggg 
      gcatccccg gatgttttc gttgcagaa aattgtgga 
      gatttccta ttataatat ggggaggat caggttgca 
      gtggtcgag gatttggca ggttttggg ggagcattt 
      taatacagt gggctgtaa taccaacga ggagatttg 
      tttctttgc ggtggatag tttaagaca gctgtccat 
      gaagtttat tgagaaggg ggagtttcc gaattttcg 
      cttccagaa ggattttct aaggccgtt tgagcatat 
      catgaagaa gaatgtgtc gccgacgat cagggatat 
      ggtggttcg ttgtgtggc ccagatggt gaactccca 
      ggccagcaa cataaaatc cggctggaa gaacatctt 
      ctcggtgtt ccacttggc cgccagcga ccaggagga 
      gtcaatcgt cgagctcgc tttccaaac aaccggcaa 
      aatcatcac cgaactcta cgacaagca gttcgcctc 
      catgatcga ctcgttcca agacgccgt caagtgcct 
      ctccgaatt cgcttgcaa cgcccgttt cctggacac 
      ctccatgga ggcgatccg cctggtgcg ctcctgcgc 
      caacagcgt cagctcctg cgcccacct cttcgccga 
      gcacgccgg catggagaa cgacgtcac cgtcgccga 
      ggaggaccg catctgggt gcggggctg gttcccgct 
      gctcttctc cctctcgtg tgtggtgaa tcggtgcaa 
      gttggacgt gcggacgcg cgctttgac cgttctttt 
      cgaaataat caaaaccta cggcgacac gttcgcgag 
      tcactggtg gaaggattt gtttaagat tttgttccg 
      gattttcga taatatgaa gctgcccga acagcatac 
      ggagaaggc cgagtggat gacgaccac ttgtaatca 
      tgccttgta cgcgattgt tgacgtttt cacgcagta 
      ttttgacgt tttgggacc gctgttgtt agatgagtt 
      gtactcgca gttgcattg gtgtgtgtt acaggataa 
      tgaacagtt ggccaagtc ggggaccaa ttgtttgga 
      gaatttggt taattcaaa tggacataa gtttgatga 
      ggtcacgtg ggaccgcac gtgccgttg catgctgga 
      cattttcga ctcgacagt ccccacagc cctcttgac 
      ctggaagcc cgaatggat gaagacaac agccgtaat 
      cgaacaaaa cggcgatgt taaaggcat tcttaaaaa 
      accaccaac ctacgaccg aaccaattc ggacgccac 
      tctgttcaa taaactggc aatcaagag tgcggtcca 
      gctggaact gattcaaac cattgacaa catcgtctt 
      ctatccagc aacttcacg gaaagaaga cgccgaaac 
      tttggcact agctgcagc cgacatggc cggaaacgg 
      ttccttgac cgagtgcca aagggagga acaggggat 
      gtaccgagt tttgacatc accgcatct tttacagtt 
      gactgattg tctcctcca gtcgcacag atttgccaa 
      agcgtttaa tctcgacca cgagcagag gaatttgct 
      gtggaaggc ggggtatcg aggttcgac taaaccgaa 
      tttgatgaa acaggaaac gcatagttt ggcttgttc 
      gttaaggat tctgttcaa aatgtattg tgatgagag 
      tcgaaggca gcactggtc gttgattga gacgaaatt 
      gattggagt gtgtcagga ggcgttgga ttatttttt 
      gaagttgca aagtgaggc acataggga ggagtggac 
      catgttgtt gttgttggt gttgacgag actgcttaa 
      aatgccaga cgataggtt cgccgtgca cgtgtcgag 
      atattaccc ccttctgtg cgaaatcgt ctgtttcga 
      tctcaaagc cgagctcag gtccatcct gcgacgagt 
      tttcctgag gataggacc ggtgtttca catcacgac 
      ggcacagtg actaaagtg attttacca tttaacctg 
      aatttttta tcaatgtta caagtttat tatttattt 
      aaaaatcct aaactttaa gtaaccgtt ttgtgtgta 
      tttttatca ttctctcaa cttaatcgc ttactagga 
      acacctctg aacgaacag tgagactca gcttacgtc 
      gtagaaatg atcattcct tgcatgaag tgattttta 
      ggagccaat aaatactac aaaagtata agttacgtg 
      ccttcaatc aaattattt atgtatgat aattgtaat 
      tagtcattc ggtatgtgg ttatgttaa tgtgtacta 
      tcatctgtt ataaatagc aaactcgtg taaataatc 
      atgaaatac tactttatt tagtcatat tcgctactg 
      ttatacaat aataaaagt aaatatatc at
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