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Gene information
General information
TC004370 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7036936..7038691
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004370
    iBeetle screen iB_09815

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004370 RA
      MEEKIPHLS LHVNEDDIN AGAARVLAA IRPHWGRRV 
      DFKLLTDGI TNKLVGCRG EEGETVLVR VYGNKTDLL 
      IDRKAETRN ILLLSRLRL APSLYATFE NGLAYEYVP 
      GCTLSPTMA KNPKIAHLV ASHMGKLHK VQVPDISNP 
      QPLLWPKIR NFLDLVPEQ FSDITKNER YHKIGAPTK 
      MQLEQEFSF LQRNLSKEK CPIVFCHND LLLGNVIYN 
      SEKDQVTFI DYEYANYNY QAFDIANHF LEFAGVENV 
      DYGNYPTRE FQIFWLGCY LNEFQPDAS QSQLELLLN 
      QVDKFTLAS HLFWGIWAL IQTEHSDIA FDFLGYAVI 
      RFNEYFKKK EQLENLN
    CDS sequence
    • >TC004370 RA
      atggaagaa aaaattccg cacttatcg ctgcatgtg 
      aacgaggat gatataaat gcgggggcg gctcgcgtg 
      ttggcggcg ataaggccg cattggggg cggcgtgtt 
      gactttaag ctgttgaca gatggcatt acgaataaa 
      ttggttgga tgtagggga gaggagggt gaaacggtg 
      ctggtgagg gtctacggg aataagact gaccttctg 
      attgatcgc aaggccgaa acgaggaac attttactt 
      ttgagccgt ttgaggctc gcccccagt ctttacgcc 
      acctttgaa aatggttta gcgtacgaa tacgtccct 
      gggtgcact ctgagcccc accatggca aaaaaccca 
      aaaattgct catctggtg gccagccat atgggcaaa 
      ttacacaaa gtccaagtc ccagacatt tccaatcct 
      cagcccctc ctctggccc aaaatccgg aattttctc 
      gacctagta cctgaacaa tttagtgat attaccaaa 
      aacgagagg taccacaaa atcggggca cccacaaaa 
      atgcaactc gaacaagaa tttagtttt ttacaaaga 
      aatttaagc aaggaaaag tgcccgatc gttttttgt 
      cacaatgat ttactccta ggcaatgtg atttataat 
      tcggagaag gatcaggtc acctttatc gattacgaa 
      tacgccaac tataattat caagcgttc gatatcgct 
      aatcatttt ttggaattt gcgggtgtg gaaaatgtc 
      gattatggg aattatcca acgcgggaa tttcaaatt 
      ttttggctt ggttgttac ttgaatgag tttcaacct 
      gacgctagc caatcacag cttgaatta ttgctcaat 
      caagtcgac aagtttacg ctcgccagt catttgttt 
      tggggcatt tgggcgctc attcaaact gaacattcc 
      gacattgcg ttcgatttt ttagggtat gccgttatc 
      agattcaat gaatatttt aagaagaag gaacagttg 
      gaaaaccta aattga
    mRNA sequence
    • >TC004370 RA
      cactaacta tgtcagaac acttattgc ctagcaacg 
      gcgcgcgaa aaattgcat catttgttt aatgtagtc 
      agcagcatg ggcgacttg attgccttg acaattgca 
      caattttcc gcattttct aggagaaaa ttgggttag 
      caatgttgc caaccggca aaacacgcc acgatcaat 
      atttgaaat atgtagtgg cacagaaaa agagaaaaa 
      ttccgctca ttctgacaa tagttaaat tgctgcaat 
      tgttatcat tttattagt gttagtgta cattgcgtg 
      agtgttgag tgtggacgt gtcattcat aagttgaaa 
      tggaagaaa aaattccgc acttatcgc tgcatgtga 
      acgaggatg atataaatg cgggggcgg ctcgcgtgt 
      tggcggcga taaggccgc attgggggc ggcgtgttg 
      actttaagc tgttgacag atggcatta cgaataaat 
      tggttggat gtaggggag aggagggtg aaacggtgc 
      tggtgaggg tctacggga ataagactg accttctga 
      ttgatcgca aggccgaaa cgaggaaca ttttacttt 
      tgagccgtt tgaggctcg cccccagtc tttacgcca 
      cctttgaaa atggtttag cgtacgaat acgtccctg 
      ggtgcactc tgagcccca ccatggcaa aaaacccaa 
      aaattgctc atctggtgg ccagccata tgggcaaat 
      tacacaaag tccaagtcc cagacattt ccaatcctc 
      agcccctcc tctggccca aaatccgga attttctcg 
      acctagtac ctgaacaat ttagtgata ttaccaaaa 
      acgagaggt accacaaaa tcggggcac ccacaaaaa 
      tgcaactcg aacaagaat ttagttttt tacaaagaa 
      atttaagca aggaaaagt gcccgatcg ttttttgtc 
      acaatgatt tactcctag gcaatgtga tttataatt 
      cggagaagg atcaggtca cctttatcg attacgaat 
      acgccaact ataattatc aagcgttcg atatcgcta 
      atcattttt tggaatttg cgggtgtgg aaaatgtcg 
      attatggga attatccaa cgcgggaat ttcaaattt 
      tttggcttg gttgttact tgaatgagt ttcaacctg 
      acgctagcc aatcacagc ttgaattat tgctcaatc 
      aagtcgaca agtttacgc tcgccagtc atttgtttt 
      ggggcattt gggcgctca ttcaaactg aacattccg 
      acattgcgt tcgattttt tagggtatg ccgttatca 
      gattcaatg aatatttta agaagaagg aacagttgg 
      aaaacctaa attgaagat aagttattt tatgagtta 
      gattattaa atttacgca agttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY