Status
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Gene information
General information
TC002444 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29446281..29455950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002444
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09814

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002444 RA
      MSSSVAKTS KYTYRSSGG GSADVSIEY SADLSALTR 
      LEDKIRLLQ EDLEIEREL RSRIEREKA DLSVQVIQL 
      SERLEEAEG GAESQFEIN KKRDTELLK LRKLLEDVH 
      LESEETAAI LKRKHQEIV VDFQEQIDS LSKAKSRAE 
      KEKSKFQTE VYELLAQVE SLNKERLVS IKHAEKLEI 
      TISELNVRI EELNRTIVD ITSHKTRLA QENLELTKE 
      VQDIKVTLE NANYLKSQL ASQLEDARR RAEDDERRR 
      SLLEAQLHQ TEVELESVR VQLEEESEA RLDLERQLV 
      KSQGEVQVY KSKYEAECA ARAEEVEEL RRKYTARIT 
      EQEEHIEAL LVKINNLEK QKSRLQSEV EVLIIDLEK 
      ANNTARELQ KRVEQLERI NIDLKTRLE ETVNLYEQT 
      QRDLRNKQQ ELQRTVHEL DKTREQKDQ LARENKKLG 
      DDLTDARNT ISELTRRLH ELELELRRL ENERDELTA 
      AYKEAEAGR KAEELRSQR LSSEFAQFR HEAEKRLQE 
      KDEEIEAIR
    CDS sequence
    • >TC002444 RA
      atgtcttcc agcgtggcg aagacctcc aagtacacc 
      taccgctcc tccgggggc ggcagcgcc gacgtctcc 
      atcgaatac agcgcggac ctcagcgcc ctaaccaga 
      ttagaggac aaaatccga ctgctccaa gaagacttg 
      gaaatcgag agggaactc agatcaagg atcgaaaga 
      gagaaggcc gatctgagc gtgcaagtc atccagtta 
      tccgaacgt ctggaggaa gctgaaggg ggcgctgaa 
      agccagttc gaaatcaac aaaaaacgc gatactgaa 
      ttgctgaag ttgcgcaaa ctcctcgag gatgtccat 
      ctggagtcg gaagagacc gccgcgatt ttgaagagg 
      aaacaccaa gagatcgtc gtcgatttt caagaacag 
      atcgattcg ctcagcaag gccaaatcc agagccgag 
      aaggagaaa agcaaattc caaaccgaa gtctacgaa 
      ctcttggcc caagttgag agcttgaac aaggaacgc 
      ttggtttcc attaaacat gccgaaaaa ttggaaatc 
      actattagc gaactgaat gtgaggatt gaggaactg 
      aaccgtact atcgttgat atcacctcg cacaagacc 
      aggttggcg caggagaat cttgaactc accaaggaa 
      gtccaagat atcaaggta acccttgag aacgccaac 
      tacttgaag agccagctc gccagccag ctggaggat 
      gcccgccgc cgcgccgaa gacgacgaa cgccgtcgc 
      tccctcctg gaagcccaa ctccaccag acggaagtc 
      gagctcgaa tccgtccgc gtccaactg gaagaagaa 
      agcgaagct cgcctggac ctcgaacgc caactggtc 
      aagtcccaa ggcgaagtc caagtgtac aaatccaag 
      tacgaagcc gagtgcgca gcccgcgct gaagaagtc 
      gaagaactc cgtcgcaag tacaccgcc aggatcacc 
      gaacaggaa gaacacatc gaagctctc ttggtcaag 
      atcaacaac ttggaaaaa caaaaatcg cgactccaa 
      tccgaagtc gaagtcctg atcatcgac ttggaaaag 
      gccaacaac acggccagg gaactccaa aagagagtc 
      gagcagttg gagagaatc aacatcgat ttgaagact 
      cgcttggaa gaaaccgtc aatttgtac gaacagacg 
      caaagggat ctccgtaac aaacaacag gagttgcaa 
      aggactgtc catgagctg gacaagacc agggaacag 
      aaggatcaa ctcgctaga gaaaacaag aagttgggt 
      gatgatctc actgatgcc aggaacacc atcagtgag 
      ttgaccagg cgtttgcac gagttggag ttggaactc 
      cgtcgcttg gagaatgag agggatgaa ctcacggct 
      gcgtacaag gaagccgaa gctggacgc aaagctgag 
      gaactcagg tctcagaga ttgtcttct gagttcgct 
      cagttccgc cacgaggcc gagaaacgc ctccaagag 
      aaggatgag gagattgaa gccatccgg taa
    mRNA sequence
    • >TC002444 RA
      agctacgcg acgccagcg tcggcacgt gataaatat 
      agcttcggg cgttttcgg tttttatcg cttgatggc 
      caagatacg gcattccgg gcgtcgcga cgctcatac 
      gaacgactc agccaccac tgtcaggcg gcgcagcac 
      gcgggtttc ttaaacact gaaatttcg acggtcagt 
      gcatcaaac gacgagctt ttcctccgg aaagcctga 
      agatgtctt ccagcgtgg cgaagacct ccaagtaca 
      cctaccgct cctccgggg gcggcagcg ccgacgtct 
      ccatcgaat acagcgcgg acctcagcg ccctaacca 
      gattagagg acaaaatcc gactgctcc aagaagact 
      tggaaatcg agagggaac tcagatcaa ggatcgaaa 
      gagagaagg ccgatctga gcgtgcaag tcatccagt 
      tatccgaac gtctggagg aagctgaag ggggcgctg 
      aaagccagt tcgaaatca acaaaaaac gcgatactg 
      aattgctga agttgcgca aactcctcg aggatgtcc 
      atctggagt cggaagaga ccgccgcga ttttgaaga 
      ggaaacacc aagagatcg tcgtcgatt ttcaagaac 
      agatcgatt cgctcagca aggccaaat ccagagccg 
      agaaggaga aaagcaaat tccaaaccg aagtctacg 
      aactcttgg cccaagttg agagcttga acaaggaac 
      gcttggttt ccattaaac atgccgaaa aattggaaa 
      tcactatta gcgaactga atgtgagga ttgaggaac 
      tgaaccgta ctatcgttg atatcacct cgcacaaga 
      ccaggttgg cgcaggaga atcttgaac tcaccaagg 
      aagtccaag atatcaagg taacccttg agaacgcca 
      actacttga agagccagc tcgccagcc agctggagg 
      atgcccgcc gccgcgccg aagacgacg aacgccgtc 
      gctccctcc tggaagccc aactccacc agacggaag 
      tcgagctcg aatccgtcc gcgtccaac tggaagaag 
      aaagcgaag ctcgcctgg acctcgaac gccaactgg 
      tcaagtccc aaggcgaag tccaagtgt acaaatcca 
      agtacgaag ccgagtgcg cagcccgcg ctgaagaag 
      tcgaagaac tccgtcgca agtacaccg ccaggatca 
      ccgaacagg aagaacaca tcgaagctc tcttggtca 
      agatcaaca acttggaaa aacaaaaat cgcgactcc 
      aatccgaag tcgaagtcc tgatcatcg acttggaaa 
      aggccaaca acacggcca gggaactcc aaaagagag 
      tcgagcagt tggagagaa tcaacatcg atttgaaga 
      ctcgcttgg aagaaaccg tcaatttgt acgaacaga 
      cgcaaaggg atctccgta acaaacaac aggagttgc 
      aaaggactg tccatgagc tggacaaga ccagggaac 
      agaaggatc aactcgcta gagaaaaca agaagttgg 
      gtgatgatc tcactgatg ccaggaaca ccatcagtg 
      agttgacca ggcgtttgc acgagttgg agttggaac 
      tccgtcgct tggagaatg agagggatg aactcacgg 
      ctgcgtaca aggaagccg aagctggac gcaaagctg 
      aggaactca ggtctcaga gattgtctt ctgagttcg 
      ctcagttcc gccacgagg ccgagaaac gcctccaag 
      agaaggatg aggagattg aagccatcc ggtaagtcc 
      aaaattgca taaccaggc agaatcaga atattttca 
      tggaataag ttaaactag ttactactg cgttagttt 
      tttacttcc taaagacct ctgatgaat tccttaatt 
      catttatga gatacaatg tggatacgg ttctttgac 
      agctttgat tggattcta gaaagtaaa aagacttcc 
      agaaaagaa acctttgca tttattatt attaagttg 
      ctcaaaata tcctctaaa tagctatga acttttttc 
      ctaatgtcc atctagtgt ttttccgat ttttgcaaa 
      aataaagct tactaaacg ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY