Status
Please wait ...
Gene information
General information
TC003978 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29462357..29467899
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003978
    iBeetle screen iB_09813

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003978 RA
      MSTTQSDIP SSDIIASLM KDPVKKAQL LQLLVQEKS 
      GFEAYKRQE ETKKTPLKN VKRRVHCES PTAVLRRRM 
      LEQKDQDST SDSSDLSET ANEVKGPKL PFGELLKGV 
      RAFVDVNRD GVDKSEGIK AVMKLMGAT IREQFTRDV 
      THVIFKDGS FTTFEKARL MKIHLVSVL WLDAVRRNS 
      ARVPEKNYP AFGSRVNDA NVSIMCSQL QKDYEDIIK 
      DEKRRTWSS LPKPKQSTE QNRRRTMLP QTQTSMSSY 
      ATAQNKPLS DSSDSEVAS ILNSEQAKS PELTSDDSE 
      CGIIDGCSS KRSKDQTDI LQSSSFLSE STDSTSLLS 
      RITTRKLRK TSVVSDMDI TPNLDERSR RSSSLRVSS 
      ETDKTKSGL RDSKSSEIN KRRKQGNTS VPSVQSSTM 
      SSLRISSGT DKTKSGLED SKSSETNKS QKQGSVSSV 
      QSSIMSSLK ISSGTDKTK SGLGDSISG KENCSVMLE 
      RTSLTDKPE EGRSVGKRR AFKSLEERS NTSSTTDSG 
      KTPKKKKPS GPSPSFTRI TKRKSKVES SESDFETKR 
      GKTKKNSSL DSGAESDVE SSIRSETSR RSRRSVSVA 
      LFTDTPPDR NRAASVSRA QSVRSTSTR NIRSRSKSP 
      SESEAVQNP TPAKKTRKL YNPDDVNVS VFLTPVNEK 
      EQEGHRRLK PQVTPKDLF VNPNNINIR IGPSGKKAP 
      KQQKKIETP PSDDENGSL LDLLNTSER RRSQRLTLS 
      QTKPKYVFT SDESQSTSS LPITPIKRR STMDFVPIS 
      QTQKRKLKL DPKRKTSIV CTKFHSSEV VQFESIVNT 
      LGRFFVEDV VTSRTTHLV AGEAKRTIN MLKAIARGC 
      WILKHEWLL KSHEQGTWL NEEDYELTE YSPAVQMCR 
      LQREAFGPS YTMDIFKDC GCIYLGQST SPKASDLKA 
      LITICQGKV TAVLKNASI VVGEYIEGD DLTCVTEVW 
      VLDSIHVNK LKSVKNYLI KAPNETSPA F
    CDS sequence
    • >TC003978 RA
      atgtcgaca acgcaaagc gacattccc tcgtcggac 
      ataatcgcc agtttgatg aaagacccc gtcaaaaaa 
      gcccaactc ttgcagtta cttgtccag gagaaaagt 
      ggcttcgaa gcctacaaa cgccaggaa gaaacgaaa 
      aaaacgcct ctaaaaaat gtcaaacga agggttcat 
      tgtgaaagc cctacggcc gtgctgcgt aggaggatg 
      ctcgagcag aaagaccaa gactcgacc tccgattct 
      tcggatttg agtgagaca gctaatgag gttaagggc 
      cccaaactg ccctttggc gagctgttg aaaggggtg 
      agggctttc gtggacgtt aatagggat ggggttgac 
      aagtctgaa ggcatcaaa gctgtgatg aagctgatg 
      ggggccacg atcagggag cagttcacg agggacgtc 
      acgcatgtt atttttaaa gacggttct tttacaacc 
      ttcgaaaaa gccagactt atgaaaatc catctagtt 
      tctgtttta tggctcgac gctgttcgt aggaacagc 
      gctcgagtc cctgaaaaa aattatccc gcttttgga 
      agtagagta aacgatgct aatgtctca attatgtgt 
      tcgcaattg caaaaagac tatgaagac ataatcaaa 
      gacgagaaa cgtcgcact tggtcttcg ttacctaaa 
      ccaaaacaa tccactgaa caaaacaga cgtcgtacg 
      atgcttcct cagactcaa acctcaatg agcagttat 
      gccactgct caaaataaa ccattgtca gatagtagc 
      gatagcgaa gttgcttcg attttaaat agtgaacaa 
      gcgaaatcg cccgaattg acttcggac gattcagaa 
      tgtggtata atcgatggt tgtagcagc aaacgctct 
      aaagaccaa acagatatt ttacaaagt tcttcgttt 
      ctgtcggaa tccaccgat tccacttct ttactcagc 
      cgaattact acacgaaaa ctaagaaaa acgagtgtt 
      gtctctgat atggacata actccaaac cttgatgaa 
      cgaagtcga cgatcgagt tctttgaga gtctcttca 
      gagactgat aaaaccaaa agtggtctc agagattca 
      aaatcttca gaaattaat aaaagacgg aaacaggga 
      aatacaagt gttccttct gtacaatcg agtactatg 
      tcgtctttg aggatctcc tcagggact gataaaact 
      aaaagtggt ctcgaagat tcaaaatct tcagaaact 
      aataaatca cagaaacaa ggaagtgtt tcttctgta 
      cagtcgagt attatgtcc tctttgaag atctcctca 
      gggactgat aaaactaaa agtggtctt ggtgattcg 
      atcagtggc aaggagaat tgttctgtt atgttggaa 
      agaacgtct ctgactgac aaacctgaa gaagggcga 
      agtgtcgga aaaagaaga gcgttcaaa agtttggaa 
      gaaaggagc aatactagt tccaccact gattccgga 
      aagacacca aagaagaag aaaccatca ggaccaagt 
      cccagtttt actcgaata actaagcgg aagtctaaa 
      gttgaatca tcagagtct gattttgaa actaaacgt 
      ggtaaaact aagaagaat agttcgcta gattcgggt 
      gcagagtca gatgtggaa agttccatt agaagtgaa 
      acgtccagg aggagtcgt cgctcagtt tctgtggct 
      ttatttact gacacccca cctgacaga aacagagct 
      gcttctgtt agtagggct caaagtgtt agaagtact 
      agtactaga aatattagg agccgatct aaatcaccc 
      agtgagagt gaagcggtt caaaatccc actccggcg 
      aagaaaact cgcaagttg tacaatcct gacgatgtc 
      aacgtttcg gtattttta acgcctgta aacgaaaaa 
      gaacaagag ggtcacagg aggctaaaa ccgcaggtt 
      actccaaag gatttattt gttaatccc aacaacatc 
      aatattcgg attgggcca agtgggaaa aaagcacca 
      aaacagcaa aagaaaatt gaaactccc ccaagtgat 
      gacgaaaat gggtcactg ttggatttg ttaaatact 
      agtgaacgc aggcgatcg caacgtttg actttgtcg 
      cagaccaaa ccaaaatac gtttttact tctgacgaa 
      agtcaatcg actagtagt cttcctatt actccgatc 
      aaaaggcgg agtactatg gattttgtc ccgatttcg 
      caaacacag aaacgtaaa ttgaagcta gatccaaag 
      aggaagact agcattgtt tgtactaaa ttccactcg 
      agtgaagtg gtccaattt gaaagtata gtcaatact 
      ttgggacga ttttttgtt gaggatgtt gtgactagt 
      aggactact catctggtc gcgggtgag gctaaaagg 
      acgattaat atgctgaag gctattgct agggggtgc 
      tggatttta aaacacgaa tggttgttg aaatcgcac 
      gagcagggg acgtggttg aatgaagaa gattacgaa 
      ttgactgaa tattcacct gctgttcag atgtgtcgg 
      ttgcaaaga gaagctttc ggtccttct tacaccatg 
      gatattttt aaagattgt ggttgtatt tatttggga 
      cagtccaca tcacccaaa gcttcagat ttgaaggct 
      ttgattact atttgtcaa gggaaagtg acggcggtg 
      cttaagaac gcgtctatt gtcgttgga gagtatatt 
      gaaggtgac gatctgact tgtgtgacg gaagtttgg 
      gtcttggat tcgatccat gtgaataaa ctaaagtcg 
      gttaaaaat tatttgatt aaagctccc aacgagact 
      agtccggct ttttag
    mRNA sequence
    • >TC003978 RA
      cgtgataat ttaatgcca ttttgtgcc tttagaaga 
      gttaggggt gattagggg gcgattttg gtaattatt 
      tgtggtgga ttggcccag ttggtgata agttccccg 
      ttggtagca ctgtttaaa agtcgcttg gcgggatcg 
      tagtgtgct gtgatggtg ttttggtaa acagtcaaa 
      aatgtgata ttttgtgat tttcctcgt ttcctgtct 
      gtgttttca acttaaaac atgtcgaca acgcaaagc 
      gacattccc tcgtcggac ataatcgcc agtttgatg 
      aaagacccc gtcaaaaaa gcccaactc ttgcagtta 
      cttgtccag gagaaaagt ggcttcgaa gcctacaaa 
      cgccaggaa gaaacgaaa aaaacgcct ctaaaaaat 
      gtcaaacga agggttcat tgtgaaagc cctacggcc 
      gtgctgcgt aggaggatg ctcgagcag aaagaccaa 
      gactcgacc tccgattct tcggatttg agtgagaca 
      gctaatgag gttaagggc cccaaactg ccctttggc 
      gagctgttg aaaggggtg agggctttc gtggacgtt 
      aatagggat ggggttgac aagtctgaa ggcatcaaa 
      gctgtgatg aagctgatg ggggccacg atcagggag 
      cagttcacg agggacgtc acgcatgtt atttttaaa 
      gacggttct tttacaacc ttcgaaaaa gccagactt 
      atgaaaatc catctagtt tctgtttta tggctcgac 
      gctgttcgt aggaacagc gctcgagtc cctgaaaaa 
      aattatccc gcttttgga agtagagta aacgatgct 
      aatgtctca attatgtgt tcgcaattg caaaaagac 
      tatgaagac ataatcaaa gacgagaaa cgtcgcact 
      tggtcttcg ttacctaaa ccaaaacaa tccactgaa 
      caaaacaga cgtcgtacg atgcttcct cagactcaa 
      acctcaatg agcagttat gccactgct caaaataaa 
      ccattgtca gatagtagc gatagcgaa gttgcttcg 
      attttaaat agtgaacaa gcgaaatcg cccgaattg 
      acttcggac gattcagaa tgtggtata atcgatggt 
      tgtagcagc aaacgctct aaagaccaa acagatatt 
      ttacaaagt tcttcgttt ctgtcggaa tccaccgat 
      tccacttct ttactcagc cgaattact acacgaaaa 
      ctaagaaaa acgagtgtt gtctctgat atggacata 
      actccaaac cttgatgaa cgaagtcga cgatcgagt 
      tctttgaga gtctcttca gagactgat aaaaccaaa 
      agtggtctc agagattca aaatcttca gaaattaat 
      aaaagacgg aaacaggga aatacaagt gttccttct 
      gtacaatcg agtactatg tcgtctttg aggatctcc 
      tcagggact gataaaact aaaagtggt ctcgaagat 
      tcaaaatct tcagaaact aataaatca cagaaacaa 
      ggaagtgtt tcttctgta cagtcgagt attatgtcc 
      tctttgaag atctcctca gggactgat aaaactaaa 
      agtggtctt ggtgattcg atcagtggc aaggagaat 
      tgttctgtt atgttggaa agaacgtct ctgactgac 
      aaacctgaa gaagggcga agtgtcgga aaaagaaga 
      gcgttcaaa agtttggaa gaaaggagc aatactagt 
      tccaccact gattccgga aagacacca aagaagaag 
      aaaccatca ggaccaagt cccagtttt actcgaata 
      actaagcgg aagtctaaa gttgaatca tcagagtct 
      gattttgaa actaaacgt ggtaaaact aagaagaat 
      agttcgcta gattcgggt gcagagtca gatgtggaa 
      agttccatt agaagtgaa acgtccagg aggagtcgt 
      cgctcagtt tctgtggct ttatttact gacacccca 
      cctgacaga aacagagct gcttctgtt agtagggct 
      caaagtgtt agaagtact agtactaga aatattagg 
      agccgatct aaatcaccc agtgagagt gaagcggtt 
      caaaatccc actccggcg aagaaaact cgcaagttg 
      tacaatcct gacgatgtc aacgtttcg gtattttta 
      acgcctgta aacgaaaaa gaacaagag ggtcacagg 
      aggctaaaa ccgcaggtt actccaaag gatttattt 
      gttaatccc aacaacatc aatattcgg attgggcca 
      agtgggaaa aaagcacca aaacagcaa aagaaaatt 
      gaaactccc ccaagtgat gacgaaaat gggtcactg 
      ttggatttg ttaaatact agtgaacgc aggcgatcg 
      caacgtttg actttgtcg cagaccaaa ccaaaatac 
      gtttttact tctgacgaa agtcaatcg actagtagt 
      cttcctatt actccgatc aaaaggcgg agtactatg 
      gattttgtc ccgatttcg caaacacag aaacgtaaa 
      ttgaagcta gatccaaag aggaagact agcattgtt 
      tgtactaaa ttccactcg agtgaagtg gtccaattt 
      gaaagtata gtcaatact ttgggacga ttttttgtt 
      gaggatgtt gtgactagt aggactact catctggtc 
      gcgggtgag gctaaaagg acgattaat atgctgaag 
      gctattgct agggggtgc tggatttta aaacacgaa 
      tggttgttg aaatcgcac gagcagggg acgtggttg 
      aatgaagaa gattacgaa ttgactgaa tattcacct 
      gctgttcag atgtgtcgg ttgcaaaga gaagctttc 
      ggtccttct tacaccatg gatattttt aaagattgt 
      ggttgtatt tatttggga cagtccaca tcacccaaa 
      gcttcagat ttgaaggct ttgattact atttgtcaa 
      gggaaagtg acggcggtg cttaagaac gcgtctatt 
      gtcgttgga gagtatatt gaaggtgac gatctgact 
      tgtgtgacg gaagtttgg gtcttggat tcgatccat 
      gtgaataaa ctaaagtcg gttaaaaat tatttgatt 
      aaagctccc aacgagact agtccggct ttttaggaa 
      cctttattc tacattaaa atattgacc agtcttggt 
      ctgtttgtt attttatct tttataccc acttattgt 
      tatttttgc ataatctga aataaatat atttcaact 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY