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Gene information
General information
TC002458 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29935883..29938021
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002458
    iBeetle screen iB_09812

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg not present - Number of animals showing the phenotype: 2
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    egg chamber stage sequence irregular -
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
    yolk mostly amount decreased - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: 5/5
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002458 RA
      MNYYQSMNH HMPHPQFHF SQPNPPPNR FIYVKHNFV 
      APNPAPKVL LNPHFKKKV YVNPQFKPA QNPLPPTNA 
      KPKIHINPN VLKNFAKTV NNVPKVVEP PKATTVIST 
      RNKLVRIPC NKSPEIRKR RNSVHTKYK IVRSGAKTG 
      SPNRFKLDK RQEKSANKT LIRSAKKRY VYVNRFLSI 
      DVMAKNVLL KKNPNLSKP GFVKINGIL YKKSPNSLR 
      KASDTSLLK STKSSTRRS LVKRYKLIR SSSAPSHAR 
      RVKPRPNFK AKKPTPSKL RKCNIPCPI FRKYGKCPG 
      KAAGTCFKL HNPDQIVLC TRFLQGACR NERCLLSHK 
      VSHEKMPTC KYFLDGLCS KDNCPYLHV KISPKADIC 
      RDFLEGFCK KGAECDKRH QFLCPEFEK NKKCSKRRC 
      PYPHGNTVR KNSKVLNRL AKKCSTKKA KTEIVECEK 
      NRYYKEKET EGGEEKVIK TVAKDRPKL GDLPAFIPL 
      FEAT
    CDS sequence
    • >TC002458 RA
      atgaattat taccaatca atgaatcac catatgccc 
      catccccag ttccatttc tcccaacct aacccccct 
      ccgaaccgt tttatctac gtaaagcac aatttcgtt 
      gccccaaac ccggccccc aaagtcctc ctcaacccc 
      catttcaag aagaaagtt tacgtcaac cctcagttc 
      aaaccggcc caaaacccc cttccgcca acaaacgcg 
      aaaccaaaa atccacatc aacccgaac gttttgaaa 
      aatttcgcc aaaactgta aacaacgtg cccaaagtt 
      gtcgaaccc ccaaaagca acgactgtg atatctacc 
      cgaaataaa ttagtccga attccgtgc aataaatca 
      cccgaaata agaaaacgg cgcaattcc gtacataca 
      aagtacaaa atcgtgcga agtggtgct aaaaccggt 
      tcccccaac cgtttcaaa ctcgacaaa aggcaggaa 
      aaaagtgcg aataaaacc cttattagg tcagccaaa 
      aaacgctac gtttacgta aaccgcttc ctgagcatc 
      gacgtaatg gccaaaaac gtgctcttg aaaaagaac 
      ccgaacttg tccaagccc ggattcgtt aaaatcaac 
      ggaattctg tacaaaaaa tccccgaat tccttgcgg 
      aaagcctcc gacacgtcc ctgctcaaa tccacgaaa 
      tctagcact cggcgaagc ctcgtcaaa cgctacaag 
      ctgatcagg tctagctcc gcccctagc cacgcccgc 
      agagttaag ccacgcccc aatttcaag gccaagaaa 
      ccgacaccc agcaaactg cgcaagtgc aacatcccg 
      tgccccatt ttccggaag tatggaaaa tgtccgggg 
      aaggcagca ggcacttgt ttcaagctg cacaacccc 
      gatcagatc gtcctgtgc acgaggttt ctccaaggg 
      gcgtgtcgt aatgaaaga tgcctactt tcccacaaa 
      gtttcgcac gaaaaaatg cccacgtgc aagtatttc 
      ctcgacggg ttgtgttct aaggacaac tgtccctat 
      ctccacgtg aagatcagc cccaaggcc gatatttgc 
      agggacttt ttggagggt ttctgcaag aagggcgct 
      gagtgcgac aaacgccac caattcttg tgtcccgaa 
      ttcgagaag aacaagaag tgttcaaaa cggaggtgt 
      ccatacccg catggaaac acggtaaga aaaaattct 
      aaggtttta aataggctt gccaagaaa tgttcaacg 
      aaaaaagca aaaacggag attgtcgag tgtgaaaaa 
      aaccgctat tacaaggaa aaagaaact gaaggcggg 
      gaggaaaaa gtcattaag actgtggcc aaggatagg 
      ccaaaattg ggggattta ccggctttt ataccgttg 
      tttgaggcc acgtga
    mRNA sequence
    • >TC002458 RA
      ttttgcgtt tttttgttt attaaaaat ttatttttt 
      tatgaacgt agacgcatt tatttgtgg cgcaagcgc 
      agtaaaaca aaacgtcaa aggaatgtt aaacatctg 
      atctcaatt acttcagtt tttggtgag ttttgcagt 
      catttgagt gaaaatcgg tgaacgagg atgttattt 
      taaccgttt tacacacca gaatgaatt attaccaat 
      caatgaatc accatatgc cccatcccc agttccatt 
      tctcccaac ctaaccccc ctccgaacc gttttatct 
      acgtaaagc acaatttcg ttgccccaa acccggccc 
      ccaaagtcc tcctcaacc cccatttca agaagaaag 
      tttacgtca accctcagt tcaaaccgg cccaaaacc 
      cccttccgc caacaaacg cgaaaccaa aaatccaca 
      tcaacccga acgttttga aaaatttcg ccaaaactg 
      taaacaacg tgcccaaag ttgtcgaac ccccaaaag 
      caacgactg tgatatcta cccgaaata aattagtcc 
      gaattccgt gcaataaat cacccgaaa taagaaaac 
      ggcgcaatt ccgtacata caaagtaca aaatcgtgc 
      gaagtggtg ctaaaaccg gttccccca accgtttca 
      aactcgaca aaaggcagg aaaaaagtg cgaataaaa 
      cccttatta ggtcagcca aaaaacgct acgtttacg 
      taaaccgct tcctgagca tcgacgtaa tggccaaaa 
      acgtgctct tgaaaaaga acccgaact tgtccaagc 
      ccggattcg ttaaaatca acggaattc tgtacaaaa 
      aatccccga attccttgc ggaaagcct ccgacacgt 
      ccctgctca aatccacga aatctagca ctcggcgaa 
      gcctcgtca aacgctaca agctgatca ggtctagct 
      ccgccccta gccacgccc gcagagtta agccacgcc 
      ccaatttca aggccaaga aaccgacac ccagcaaac 
      tgcgcaagt gcaacatcc cgtgcccca ttttccgga 
      agtatggaa aatgtccgg ggaaggcag caggcactt 
      gtttcaagc tgcacaacc ccgatcaga tcgtcctgt 
      gcacgaggt ttctccaag gggcgtgtc gtaatgaaa 
      gatgcctac tttcccaca aagtttcgc acgaaaaaa 
      tgcccacgt gcaagtatt tcctcgacg ggttgtgtt 
      ctaaggaca actgtccct atctccacg tgaagatca 
      gccccaagg ccgatattt gcagggact ttttggagg 
      gtttctgca agaagggcg ctgagtgcg acaaacgcc 
      accaattct tgtgtcccg aattcgaga agaacaaga 
      agtgttcaa aacggaggt gtccatacc cgcatggaa 
      acacggtaa gaaaaaatt ctaaggttt taaataggc 
      ttgccaaga aatgttcaa cgaaaaaag caaaaacgg 
      agattgtcg agtgtgaaa aaaaccgct attacaagg 
      aaaaagaaa ctgaaggcg gggaggaaa aagtcatta 
      agactgtgg ccaaggata ggccaaaat tgggggatt 
      taccggctt ttataccgt tgtttgagg ccacgtgac 
      gaatatttc ttggcaatt ttagatttt ttatactgt 
      ctttgagaa aataaaaca atgaagcaa ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY