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Gene information
General information
TC002505 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26342270..26358383
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002505
    iBeetle screen iB_09806

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002505 RA
      MGDLAEAVE DLICTFDPT GDTTMDTLA MFVFGWILA 
      ALFVLWLGK IVYARFLAR ASSSSSNET KTKIEPVSA 
      DAVDAKAVK KTATAPVKS APSGGKGGY VPPTPPVRK 
      RLTRQSPTP EVRKVKYVP APQCTGPDN VCVLWVNDV 
      LQWLYNDLV IVNELLAVW LQAMNEFTK KTAAEQGVG 
      VEFVRILPE TNPPLLTNI FAENDSKDD VTITCDCEA 
      TPALQLKSF RQKGDKVET SHYRVNINR FRARLNIFC 
      ISEKLQADV KCDGWPEIK VALAPVGNI KNNLDETQL 
      QEVINEIII NALRNTEVH LNLAQYPTS PRLIRHVNT 
      APGHILPVH YDSMSNAYT STPNHQPLQ VPGEKRLLV 
      KVVRAAQLG ASTGCTEPY CVVEIDEPP QKNQTSIKK 
      STDSPFWDE QFLFDLSQN TAEVLFEVY DHAVKPNKF 
      LGLAIVGVD ELIANPSQR QIISLQGAP YESDPVSGT 
      LTVEFLFVE GADIPNIGG GQPYKIKET IRQPLTPTN 
      SARMQNMTF QNENLTNGN HYSDRDLDN RQNISQNKN 
      TLVIHSVQR QPSQRLVKV ALTDGTWKE IERVEKTDD 
      NQEDVENLN GSVSNELNG TNESKDKDA SEAETSKPG 
      DFSNTGTLQ SQTSSDIEN RGRPRKRRD FFGTIKRRL 
      GKSRSKSVG PDNDNGRDD SMNRSVSAD RSRNDESYR 
      LNVPGQKSI DETSRRSSL SEASGLSSA STRTYINEA 
      STLVLETLE NGIKKHYLV PLSLAQKSK WRKKGIKLH 
      IFNDHTFIA RHLTGGTVC QVCGKSIAR RIGKQGYEC 
      RDCLLKCHK HCHVKVNDN CPTSTIHNI ELSYITNPY 
      TDKNIRML
    CDS sequence
    • >TC002505 RA
      atgggcgat ctagccgaa gccgttgaa gacttgatc 
      tgcacgttc gacccaact ggagacacc acgatggat 
      acgctggcc atgttcgtc ttcggctgg atcctggcc 
      gccctcttc gtcctgtgg ctggggaag atcgtctac 
      gctcgattc ttagcgaga gcatcatca tcatcatcg 
      aacgaaacg aaaacgaaa atcgagccg gtgtcggcg 
      gacgccgtc gacgccaaa gctgtgaag aagacggcc 
      accgcgccc gtgaagagc gcgcccagc ggcgggaaa 
      gggggctac gtgccgccc acgccgccg gtgaggaag 
      aggctcacg aggcagagt ccgacgccg gaagtcagg 
      aaagtcaag tacgtgccg gcaccacag tgcaccgga 
      ccggacaat gtttgtgtg ttgtgggtt aatgacgtg 
      ctgcagtgg ttgtacaac gacttggtc atcgttaat 
      gaactcttg gcggtgtgg ctgcaagcg atgaatgag 
      ttcaccaag aaaacggcg gcggagcaa ggagtcggt 
      gtggaattc gtccgtatc ctccccgag acgaatcct 
      cccctcctg acaaacata tttgctgag aacgattca 
      aaagatgac gtgacaatc acatgtgac tgtgaagcc 
      acaccagca ttacaatta aaaagcttc cgccaaaaa 
      ggtgacaaa gtcgaaaca tcacattac agagttaat 
      attaatcgc tttagggca aggttaaac atcttttgc 
      atatctgaa aaattacaa gccgacgtt aagtgcgac 
      ggttggccc gaaattaaa gtggcactt gcgcccgtc 
      ggcaacatc aaaaacaac ttggacgaa acccaactc 
      caggaagtc ataaacgag atcatcatc aacgccttg 
      aggaacacc gaggtgcac ctcaacctc gcccagtac 
      cccaccagc cccaggctc atcaggcat gtcaacaca 
      gcccccgga cacatccta cccgtccac tatgacagc 
      atgtctaat gcgtacacc tcgacgccg aaccaccag 
      ccgctccaa gtccctggc gagaagcgc cttctggtt 
      aaggtggtc cgcgccgcc cagctgggg gcttccacc 
      ggctgcacg gagccctac tgcgtggtg gaaatcgac 
      gagcccccg cagaagaac cagacttcg atcaagaaa 
      agcactgat tcgccgttt tgggacgag caattccta 
      ttcgatttg tcccaaaat acggccgaa gttttgttt 
      gaagtttac gatcatgca gtcaaaccg aataagttt 
      ctcggtttg gccatcgtt ggagtggac gaactcatc 
      gcgaatccg tcccaaaga cagattatt tcgctccag 
      ggggctccg tatgagagt gacccggtt tcggggact 
      ctcactgtc gagtttttg ttcgtcgag ggggctgat 
      attccgaat atcgggggt ggacaaccg tacaagatc 
      aaggagacg attaggcaa cctttgacg cccactaat 
      agtgcgagg atgcaaaat atgacgttc cagaatgaa 
      aatctgacc aatggaaat cattatagt gacagagat 
      ttagataat aggcagaat ataagtcag aataaaaac 
      actctggtg atacacagt gtacaaagg caaccgtct 
      caaaggctc gtgaaggtt gcactgact gacggcact 
      tggaaagag atcgaacgc gtcgaaaaa accgacgac 
      aatcaggaa gacgttgag aatttaaat ggcagcgtt 
      agcaacgaa ctaaacggc actaacgaa agtaaagat 
      aaagacgct tcagaggct gaaacatcc aagcccggt 
      gatttctcc aacactgga accctccaa tcgcagaca 
      tcctctgat atagaaaac cgaggtcgt ccgaggaaa 
      cgtcgcgac tttttcggg actatcaag cgccgattg 
      gggaaatcc cggtcgaag tcggtcgga cccgacaac 
      gacaatggc cgagatgac tcgatgaat cggtcggtt 
      tctgccgac aggagccga aacgatgaa agctatcgg 
      ctgaacgtc cctgggcag aaatccatc gacgagacc 
      tcgcgtcgg tcgagtctt tccgaggcc tcgggtctt 
      agcagcgct tcaactagg acttacata aacgaagcc 
      agtacttta gtacttgaa acgctcgaa aacggaatc 
      aaaaaacac tacttggtc ccgttatca ctcgcacaa 
      aagtccaaa tggaggaaa aaaggaatc aaactgcac 
      attttcaac gaccatacg tttattgcc aggcaccta 
      actggtggc actgtttgt caagtgtgc ggaaagagc 
      atagctcga cgaattggg aaacaaggc tacgaatgt 
      cgcgattgt ttgttgaag tgtcacaaa cactgccac 
      gtcaaggtc aacgataac tgtcccacg tccaccata 
      cacaatatt gaattgtct tacatcacg aatccctac 
      accgataag aacatccga atgctttaa
    mRNA sequence
    • >TC002505 RA
      tccaccaag tcgaaggag agaggtgga aggcaacaa 
      gggagagca tgtcccaat gcgtggtgg tatcgacgt 
      tttgaagcg agtcatggc gcattgtgc ttcaagcag 
      taatcgaaa gttgttctt tgtagtgtt gagtgtgtt 
      aatgccgac acgatatag ctttacgtt cagtgatta 
      attaaacat tgctcttcg ttatcatct tgtgcagat 
      ttgtgacag tgtgtccgt gacagggca tcgattttt 
      gagatatcc ccatcgcat ggagcacga ttcagtgga 
      agtatggcc gcgacatgg gcgatctag ccgaagccg 
      ttgaagact tgatctgca cgttcgacc caactggag 
      acaccacga tggatacgc tggccatgt tcgtcttcg 
      gctggatcc tggccgccc tcttcgtcc tgtggctgg 
      ggaagatcg tctacgctc gattcttag cgagagcat 
      catcatcat catcgaacg aaacgaaaa cgaaaatcg 
      agccggtgt cggcggacg ccgtcgacg ccaaagctg 
      tgaagaaga cggccaccg cgcccgtga agagcgcgc 
      ccagcggcg ggaaagggg gctacgtgc cgcccacgc 
      cgccggtga ggaagaggc tcacgaggc agagtccga 
      cgccggaag tcaggaaag tcaagtacg tgccggcac 
      cacagtgca ccggaccgg acaatgttt gtgtgttgt 
      gggttaatg acgtgctgc agtggttgt acaacgact 
      tggtcatcg ttaatgaac tcttggcgg tgtggctgc 
      aagcgatga atgagttca ccaagaaaa cggcggcgg 
      agcaaggag tcggtgtgg aattcgtcc gtatcctcc 
      ccgagacga atcctcccc tcctgacaa acatatttg 
      ctgagaacg attcaaaag atgacgtga caatcacat 
      gtgactgtg aagccacac cagcattac aattaaaaa 
      gcttccgcc aaaaaggtg acaaagtcg aaacatcac 
      attacagag ttaatatta atcgcttta gggcaaggt 
      taaacatct tttgcatat ctgaaaaat tacaagccg 
      acgttaagt gcgacggtt ggcccgaaa ttaaagtgg 
      cacttgcgc ccgtcggca acatcaaaa acaacttgg 
      acgaaaccc aactccagg aagtcataa acgagatca 
      tcatcaacg ccttgagga acaccgagg tgcacctca 
      acctcgccc agtacccca ccagcccca ggctcatca 
      ggcatgtca acacagccc ccggacaca tcctacccg 
      tccactatg acagcatgt ctaatgcgt acacctcga 
      cgccgaacc accagccgc tccaagtcc ctggcgaga 
      agcgccttc tggttaagg tggtccgcg ccgcccagc 
      tgggggctt ccaccggct gcacggagc cctactgcg 
      tggtggaaa tcgacgagc ccccgcaga agaaccaga 
      cttcgatca agaaaagca ctgattcgc cgttttggg 
      acgagcaat tcctattcg atttgtccc aaaatacgg 
      ccgaagttt tgtttgaag tttacgatc atgcagtca 
      aaccgaata agtttctcg gtttggcca tcgttggag 
      tggacgaac tcatcgcga atccgtccc aaagacaga 
      ttatttcgc tccaggggg ctccgtatg agagtgacc 
      cggtttcgg ggactctca ctgtcgagt ttttgttcg 
      tcgaggggg ctgatattc cgaatatcg ggggtggac 
      aaccgtaca agatcaagg agacgatta ggcaacctt 
      tgacgccca ctaatagtg cgaggatgc aaaatatga 
      cgttccaga atgaaaatc tgaccaatg gaaatcatt 
      atagtgaca gagatttag ataataggc agaatataa 
      gtcagaata aaaacactc tggtgatac acagtgtac 
      aaaggcaac cgtctcaaa ggctcgtga aggttgcac 
      tgactgacg gcacttgga aagagatcg aacgcgtcg 
      aaaaaaccg acgacaatc aggaagacg ttgagaatt 
      taaatggca gcgttagca acgaactaa acggcacta 
      acgaaagta aagataaag acgcttcag aggctgaaa 
      catccaagc ccggtgatt tctccaaca ctggaaccc 
      tccaatcgc agacatcct ctgatatag aaaaccgag 
      gtcgtccga ggaaacgtc gcgactttt tcgggacta 
      tcaagcgcc gattgggga aatcccggt cgaagtcgg 
      tcggacccg acaacgaca atggccgag atgactcga 
      tgaatcggt cggtttctg ccgacagga gccgaaacg 
      atgaaagct atcggctga acgtccctg ggcagaaat 
      ccatcgacg agacctcgc gtcggtcga gtctttccg 
      aggcctcgg gtcttagca gcgcttcaa ctaggactt 
      acataaacg aagccagta ctttagtac ttgaaacgc 
      tcgaaaacg gaatcaaaa aacactact tggtcccgt 
      tatcactcg cacaaaagt ccaaatgga ggaaaaaag 
      gaatcaaac tgcacattt tcaacgacc atacgttta 
      ttgccaggc acctaactg gtggcactg tttgtcaag 
      tgtgcggaa agagcatag ctcgacgaa ttgggaaac 
      aaggctacg aatgtcgcg attgtttgt tgaagtgtc 
      acaaacact gccacgtca aggtcaacg ataactgtc 
      ccacgtcca ccatacaca atattgaat tgtcttaca 
      tcacgaatc cctacaccg ataagaaca tccgaatgc 
      tttaatctc tggttaccg ttaatttca ttttaaacg 
      ggtagttta gtctctaat cttaagcaa actatagca 
      caacttaag ctgtacatt gaaccacat acacattgt 
      tttattttt ttgttttaa ctatatttg attatatat 
      ttttgaatt ttaagaatc acgttttag ttttaattt 
      attttgtta atgtttgtt tactatttt aaagatctg 
      ttggacgtt ttatcgcaa atgtttact aattgtatg 
      ctgatacga aattataaa tgtttctgt aagtgcctg 
      taaaaatct ttgtatttc tatttaata caaataata 
      tgctattca acaagaagc gtttcgtta agcctgagg 
      tgtaagtat tcaatattt atattattt tcttttgtt 
      aattgcata aatggaaat aaatacgtt ttaactac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY