Status
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Gene information
General information
TC032606
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002119
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20061996..20106365
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032606
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09804

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032606 RA
      MDRESIGKD AQLPLTQSK YVLAKYGIQ VIDPQHDPL 
      EETFLATGS VTDDMHYHP GPRAIECKA DAGKFGCDR 
      DSCMRVLWC YLFPCALLE SCLVALCDC WWECFSGCL 
      RRMEEHYTF QCCERCSRF YLDAPSLAS VCVCVDSVP 
      TSDLDTFLC KQEWERRLE EELDKPRRD SASQIDDFF 
      EPAHNRHQP TMSYVSMRT LPGTDDDVF LRPPLWEDI 
      ASSIQNIDP ENANMLGVS PSHVKLEAV DELTASCPP 
      TPSPLLSPL EIKTEKVLQ QTTPTLHLM GTPTSPYNQ 
      AQPTSPQQT PHYHQPQQT LHHPHNNNN NNNNNNNNN 
      THSQYKYNN AVAPSPAPG SALYPPISR LMYVSPLTP 
      PSSEPGSPG GTLPRRTPP PPYPAPGCQ QQTPQPTIN 
      RITSSTAKY NRRNNPELE KRRIHHCDF IGCTKVYTK 
      SSHLKAHQR IHTGEKPYQ CQWPECEWR FARSDELTR 
      HYRKHTGAK PFKCAVCER SFARSDHLA LHMKRHLPK 
      TSK
    CDS sequence
    • >TC032606 RA
      atggacaga gagagtatt ggaaaagac gcacaatta 
      ccattgact cagtcgaaa tatgttttg gccaaatat 
      ggaatacaa gttattgat ccgcaacat gatcctctg 
      gaagagacg tttctggcg acgggaagt gttacggac 
      gatatgcat tatcatcca ggacctaga gctattgaa 
      tgcaaagct gacgccgga aaattcggc tgcgaccgc 
      gacagttgc atgcgcgtc ctctggtgt taccttttc 
      ccttgtgcg ttgctcgaa agctgcttg gtggcactt 
      tgcgactgc tggtgggaa tgctttagc ggttgtctt 
      cgtcgcatg gaagagcac tatacgttc caatgttgc 
      gaaaggtgt tcgaggttt tacctggat gccccttcg 
      cttgcttct gtgtgcgtg tgtgtggac agcgtcccg 
      actagtgac ttggacacg ttcctgtgc aagcaggag 
      tgggaacgt cggctcgaa gaagaactc gacaaacct 
      cgacgagat tcagcatct caaatcgat gattttttc 
      gaacccgcc cacaatcgc catcaaccc accatgagt 
      tacgtttcg atgagaacc cttcctgga acagacgac 
      gatgttttt ctacgacca cccctttgg gaagacata 
      gccagtagc atacaaaac atagacccc gaaaatgcc 
      aatatgtta ggagtaagc cccagtcac gtcaaacta 
      gaagccgtt gacgaacta actgccagt tgccctcca 
      acgccgtcc cctctcctc tccccctta gaaattaaa 
      accgaaaag gtgttgcaa cagaccacc cccacccta 
      cacctgatg ggcacccca acgagcccc tacaaccag 
      gcgcagccc acgagtccg cagcaaacc ccccactac 
      caccagccc cagcaaacg ctgcaccac ccccacaac 
      aacaacaac aataacaat aacaacaat aacaacaac 
      acgcactcc cagtataaa tacaacaat gcagtggcg 
      ccgagtccc gcccctggc agcgctctc taccccccc 
      atatctagg ttaatgtac gtgtcgccg ttgacaccc 
      cccagctct gagccgggc tcgcctggg ggcacgctg 
      ccaaggcgc acgccgccc ccgccatat cccgccccc 
      ggatgccag cagcagacg ccccagccg acgatcaac 
      cgaattacg tcctcgacg gcgaagtac aataggcgg 
      aataatcca gaattggag aagagacgg atacaccat 
      tgcgatttc ataggttgt acaaaggtc tacacaaag 
      agctcacac ctgaaagca catcaaaga attcacaca 
      ggagagaag ccgtaccag tgtcagtgg cctgaatgt 
      gaatggcga ttcgccaga tcggatgaa cttacaaga 
      cattatcga aagcacaca ggagcaaaa ccatttaaa 
      tgtgcggtt tgtgaaaga tcgtttgct cgatcggat 
      catcttgcc ttacacatg aagcgacac cttcctaaa 
      acatcaaag taa
    mRNA sequence
    • >TC032606 RA
      caataatag cgtcgtgac agcaacacg ccgagtctc 
      cccgtgtgg caaccacaa gcaattacc gtaatgagg 
      tcataactc ttcccctaa aagccggtg gtggtggtg 
      gttcactga caatcccat taccaaatt acagcacgc 
      gtgtatcgc attagcctt gtctatgtg tttaaacac 
      ctcgcgtgg ttgtaaccc cctaacttg ataatttca 
      taatagcca aggggcgat aagaaacaa actaaaaga 
      gagttgtgt tttttatgt aacttcata aacagcaaa 
      ggggcggaa aaatctaga tccctgggg tgtgattcg 
      atagttttg aactgatgt caaccgatg tcatatcac 
      aaaaatgga cagagagag tattggaaa agacgcaca 
      attaccatt gactcagtc gaaatatgt tttggccaa 
      atatggaat acaagttat tgatccgca acatgatcc 
      tctggaaga gacgtttct ggcgacggg aagtgttac 
      ggacgatat gcattatca tccaggacc tagagctat 
      tgaatgcaa agctgacgc cggaaaatt cggctgcga 
      ccgcgacag ttgcatgcg cgtcctctg gtgttacct 
      tttcccttg tgcgttgct cgaaagctg cttggtggc 
      actttgcga ctgctggtg ggaatgctt tagcggttg 
      tcttcgtcg catggaaga gcactatac gttccaatg 
      ttgcgaaag gtgttcgag gttttacct ggatgcccc 
      ttcgcttgc ttctgtgtg cgtgtgtgt ggacagcgt 
      cccgactag tgacttgga cacgttcct gtgcaagca 
      ggagtggga acgtcggct cgaagaaga actcgacaa 
      acctcgacg agattcagc atctcaaat cgatgattt 
      tttcgaacc cgcccacaa tcgccatca acccaccat 
      gagttacgt ttcgatgag aacccttcc tggaacaga 
      cgacgatgt ttttctacg accacccct ttgggaaga 
      catagccag tagcataca aaacataga ccccgaaaa 
      tgccaatat gttaggagt aagccccag tcacgtcaa 
      actagaagc cgttgacga actaactgc cagttgccc 
      tccaacgcc gtcccctct cctctcccc cttagaaat 
      taaaaccga aaaggtgtt gcaacagac cacccccac 
      cctacacct gatgggcac cccaacgag cccctacaa 
      ccaggcgca gcccacgag tccgcagca aacccccca 
      ctaccacca gccccagca aacgctgca ccaccccca 
      caacaacaa caacaataa caataacaa caataacaa 
      caacacgca ctcccagta taaatacaa caatgcagt 
      ggcgccgag tcccgcccc tggcagcgc tctctaccc 
      ccccatatc taggttaat gtacgtgtc gccgttgac 
      accccccag ctctgagcc gggctcgcc tgggggcac 
      gctgccaag gcgcacgcc gcccccgcc atatcccgc 
      ccccggatg ccagcagca gacgcccca gccgacgat 
      caaccgaat tacgtcctc gacggcgaa gtacaatag 
      gcggaataa tccagaatt ggagaagag acggataca 
      ccattgcga tttcatagg ttgtacaaa ggtctacac 
      aaagagctc acacctgaa agcacatca aagaattca 
      cacaggaga gaagccgta ccagtgtca gtggcctga 
      atgtgaatg gcgattcgc cagatcgga tgaacttac 
      aagacatta tcgaaagca cacaggagc aaaaccatt 
      taaatgtgc ggtttgtga aagatcgtt tgctcgatc 
      ggatcatct tgccttaca catgaagcg acaccttcc 
      taaaacatc aaagtaata ctactatct gatatttat 
      ataacgttt tacatacct tatttatcg tcacattcc 
      tctctacta ttctcttaa ggagcgatc tcaactact 
      tagaaaacg tacattttg ttaaattcc aatccgcct 
      tatacctca tgttcaccg tcatgcctt ttgtgactt 
      agaggcctt ctccagtgt acttagaaa atataaatg 
      tgtataata atgcaaaat ttgtgattt taacttaac 
      acttctcca tccacgctt gacgtcgta ctataggtg 
      attgtaata gatttttca gtttgttca tttatttgg 
      ttgtgaagt tattgttat ttatgataa tgtttatca 
      aatgagcac taatatcgt taggtaaat tttctttaa 
      cattgccat ctgttttac ccagtgttg tgatttctg 
      ttttgttcg aatgcgtat acacttttc agtatatga 
      agttaccaa atatgtacg ctgaattat gtacataaa 
      aataaagca aaactgact agaaactag tcaagtaat 
      tccaatatt tgtaagtag gttgtaatt attatattg 
      ctactttgc gtatttata tcgtaagta ttatagatg 
      agacacaaa ttttattaa cattacttt gtacttcat 
      gttatttat atgaataga gcaactaga gtacacaat 
      attttaggt agaaattgt tttcatttt ttaattttg 
      tataattta cttttaata caagtttat ttttacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY