Status
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Gene information
General information
TC003842 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22843800..22849586
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003842
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09799

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003842 RA
      MTEGTATIR TRKFMTNRL LCRKQMVVD 
      VLHPGQASV KKTDIREKL AKMYKVTPD 
      VVFVFGFRT NFGGGKSTG FALIYDTLD 
      FAKKFEPKH RLARHGLFE KKQQTRKQR 
      KERKNRMKK VRGTKKSKV GAAAKK
    CDS sequence
    • >TC003842 RA
      atgactgaa ggtacagcc acaattcgc actcgcaaa 
      tttatgacc aacagattg ttgtgtcgc aaacagatg 
      gttgtagat gttctacat ccaggtcag gcgtctgtt 
      aaaaaaaca gatattagg gaaaaactg gccaaaatg 
      tacaaagtt acgccagat gtagtattt gtattcggt 
      ttccgtaca aacttcggc ggtggcaaa tcgacaggt 
      tttgcgttg atttacgac acgttagat tttgccaag 
      aaattcgag cctaaacac aggcttgcc cgtcacggt 
      ctcttcgaa aagaaacaa caaacgcgc aaacagcgt 
      aaagaacgt aagaacaga atgaagaaa gtacgcggt 
      accaagaag agtaaagtg ggtgctgcg gctaagaag
    mRNA sequence
    • >TC003842 RA
      agtagtttt acttgtcgg tctttttaa cctactcga 
      tactttcgt agagggcgc tcttttcgt gcagtaatt 
      tttgtcatc aaaatgact gaaggtaca gccacaatt 
      cgcactcgc aaatttatg accaacaga ttgttgtgt 
      cgcaaacag atggttgta gatgttcta catccaggt 
      caggcgtct gttaaaaaa acagatatt agggaaaaa 
      ctggccaaa atgtacaaa gttacgcca gatgtagta 
      tttgtattc ggtttccgt acaaacttc ggcggtggc 
      aaatcgaca ggttttgcg ttgatttac gacacgtta 
      gattttgcc aagaaattc gagcctaaa cacaggctt 
      gcccgtcac ggtctcttc gaaaagaaa caacaaacg 
      cgcaaacag cgtaaagaa cgtaagaac agaatgaag 
      aaagtacgc ggtaccaag aagagtaaa gtgggtgct 
      gcggctaag aagtaaaaa ccgtggaca gcatccaac 
      agcacttca ttgtgtggc atgtatatt ataccaaat 
      agggatttt tatatttga aacacaata aacatctaa 
      gtgctcatt aaattatta tggaattac atcctttcc 
      atatattat atttttaag aatttttaa ataaattga 
      cagcaattt acccttatt ttattgaaa gctccactc 
      gcttcactt accaggcta attaaactt caatttagt 
      tcgctaatt gagttaaat atcgactga agtagaggt 
      aaggagatg aggctctca aggcactat ttttattaa 
      aaaatcagt tttaattga aaaagtgta aaaataaag 
      ctcttgttt tcaagggaa ctcgaagac tggacacta 
      gaaacaaac acataacat taagtagtt atgaaaaag 
      tgaatacct ctttttgtc gtgacaggc atttgataa 
      actcggacg caatatcgc aagcacttt gtggcttac 
      ttgcgtctg aatcatcag ctggttttt cagttgtgc 
      tttcaggat tttcctgtg attcgtcct tgtccactt 
      gatccgcat agaagtaga cttcactca cttgaaaaa 
      tggtcataa atttataaa attgaaata tgcaactta 
      cgtgttttg ttgttaaga gcgtcgtag catatttcc 
      aaccgtcca atgaaatca atggcttgg ttgtatatt 
      acattgttt tttgcccat tttcgtatt tcttcgcag 
      gcagggtaa tcattagcg cttagctag aaatgatgg 
      ttaaagctt attataata aaggactga catttgtaa 
      gggcaataa aaagtggtt tatgtgagt tgttataag 
      ggacgggga aaggataac gcactcgtg tgagggtga 
      agctgttta gtttatggg aacattgtt tttttttag 
      taaatattt cggctaacg aatggaaaa cgataacaa 
      cttatgttt aaagcaaat atctaatct aaattacta 
      atcacattt attatcatt atttgattt ttacctagt 
      ttataataa aattttacc aaaactgtc agaacaatg 
      caattgaat tagtatttt ttgttgcat ttaagaaaa 
      attcggaaa tatatcata actgacttt gcaggagta 
      ttattcttt tatattttt ggttaaatt gtggtaaaa 
      actgaattt ttaagtata aatcatact aacaaagtt 
      attcattga aaaattatt gaggaaaaa gcagcaact 
      gcaagacca ctttcataa ttaacaaag cacggattt 
      tttccttca attatgatc atgcacata ttaacctct 
      gacctcatc ctacaacag tgtcatgct gtcttcgag 
      caacacatt ccttcgtgt tacgcttaa tgtgagaaa 
      caattttct tcctttctg aaacggtat tttagggta 
      ttttaggtc tctactcat cttagtttt gaacctacg 
      tactcattt tttactgaa tattttatt aaacagctt 
      ccaattttt aatacagca gattcaact tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY