Status
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Gene information
General information
TC011241 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 93337..97298
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011241
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09792

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011241 RA
      MGFVKVVKN KQYFKRFQV KFKRRRQGK TDYYARKRL 
      IVQDKNKYN TPKYRLIVR LSNRDITCQ VAYSRIEGD 
      KIVCAAYSH ELPKYGIKA GLTNYAAAY CTGLLLARR 
      LLKQLGLDK LYAGTTEVT GDEYNVEGV ENGPGAFRC 
      YLDVGLQRT TTGARVFAA MKGAVDGGL NIPHSTKRF 
      PGYDAESKS FNADVHRSH IFGQHVADY MRSLEEEDP 
      EAFKRQFSQ YIKLGINAD QIEGIYKNA HAAIRANPT 
      AEKKPEKAP SATKKRWNR KKLTLAERK NRVAQKKKS 
      FIAKIQEGQ AEP
    CDS sequence
    • >TC011241 RA
      atgggtttc gtgaaagtg gtgaaaaac aagcaatac 
      ttcaagcgt ttccaagtt aagtttaag aggcgcagg 
      cagggaaag accgattac tatgcgcgt aagcgtctc 
      attgttcaa gataagaac aagtacaac acccccaag 
      taccgcctg attgtccgt ttgtccaac cgcgatatt 
      acttgtcag gtggcttat tcgcgtatc gagggcgat 
      aaaattgtg tgcgctgcg tactctcat gagttgccc 
      aagtacggg attaaggct ggacttacc aattatgct 
      gctgcttat tgcactgga ttgcttttg gccaggcgt 
      ttgctgaag caattgggg ttggataag ttgtatgct 
      ggcaccacc gaagtcact ggggatgag tacaatgtg 
      gaaggtgtt gagaacgga cctggcgct ttcaggtgc 
      tacttggat gtgggtctc cagcgtacc acaaccggt 
      gctcgtgtt ttcgctgca atgaaaggt gcagtcgat 
      ggaggtcta aacattcct cactctacg aaacgattc 
      cctggttat gacgctgaa tctaaatca ttcaatgct 
      gacgtacac cgttctcat attttcggc caacatgta 
      gctgattac atgcgttcg ttggaggag gaagaccct 
      gaggccttc aagagacag tttagtcaa tacatcaag 
      ttgggcatt aacgctgac cagattgaa ggaatttac 
      aagaacgcc cacgccgct attcgagcc aatccaaca 
      gctgaaaag aaaccagaa aaggctcca tcagctacc 
      aagaagagg tggaaccgc aagaagctc actctggcc 
      gaacgcaag aaccgtgtc gcccaaaag aaaaaatct 
      ttcattgca aagatccaa gaaggacag gctgagcct 
      tag
    mRNA sequence
    • >TC011241 RA
      cgcggtggc tgacgagcg cctcgtagt gtcctttgt 
      gcttaattt ttcaattaa ttacccaaa atgggtttc 
      gtgaaagtg gtgaaaaac aagcaatac ttcaagcgt 
      ttccaagtt aagtttaag aggcgcagg cagggaaag 
      accgattac tatgcgcgt aagcgtctc attgttcaa 
      gataagaac aagtacaac acccccaag taccgcctg 
      attgtccgt ttgtccaac cgcgatatt acttgtcag 
      gtggcttat tcgcgtatc gagggcgat aaaattgtg 
      tgcgctgcg tactctcat gagttgccc aagtacggg 
      attaaggct ggacttacc aattatgct gctgcttat 
      tgcactgga ttgcttttg gccaggcgt ttgctgaag 
      caattgggg ttggataag ttgtatgct ggcaccacc 
      gaagtcact ggggatgag tacaatgtg gaaggtgtt 
      gagaacgga cctggcgct ttcaggtgc tacttggat 
      gtgggtctc cagcgtacc acaaccggt gctcgtgtt 
      ttcgctgca atgaaaggt gcagtcgat ggaggtcta 
      aacattcct cactctacg aaacgattc cctggttat 
      gacgctgaa tctaaatca ttcaatgct gacgtacac 
      cgttctcat attttcggc caacatgta gctgattac 
      atgcgttcg ttggaggag gaagaccct gaggccttc 
      aagagacag tttagtcaa tacatcaag ttgggcatt 
      aacgctgac cagattgaa ggaatttac aagaacgcc 
      cacgccgct attcgagcc aatccaaca gctgaaaag 
      aaaccagaa aaggctcca tcagctacc aagaagagg 
      tggaaccgc aagaagctc actctggcc gaacgcaag 
      aaccgtgtc gcccaaaag aaaaaatct ttcattgca 
      aagatccaa gaaggacag gctgagcct tagattttt 
      atttctttg tattatata aaagattga gtgtgatca 
      ataaatgtt ggtgcacgg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY