Status
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Gene information
General information
TC032517
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010539,TC010538
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14840554..14850449
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032517
    iBeetle screen iB_09791

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032517 RA
      MLTAVPDAR KQILKEERY NSTNFQTLW DFGGVFKYS 
      PLFYGWYTY KESKKSGYR MPFAYFITG LVAYAYSFF 
      ATLRKMAEN SRMSKLSEK DDECIFSWK LFTAWDYMI 
      GNAETAHNR IASIVVGFK EALLEEAEK KREARNWKI 
      TGFRILVNT VVLGLLFFS AFAVILVVK RSTEPEARS 
      SVWRSNEIT FVMTFISIS FPMIFEGLG FLEQYHPRK 
      QLRLQLARI MVLNLLNLY SLIFALFDK INDMTPELE 
      SFKRNLTRN NTSVLAYSA GMQCYNSCN SETDTLKAF 
      NLIAAVATN VSFTMYPVM QKAVKNATT EPDKETIDA 
      EFTTGMYTN PMDFLDNYT TELPGNFSS DDSTTFKIS 
      TNLPDFVTN SLSTSDGTE ESTSASADL ILSTATINE 
      TSADKPKLQ GSAVKKRNC LEICENFTV TPSPIIDES 
      SLNYTTRRR LRNLCWETN FGQELIKLT VMDLVMTIL 
      STLAMDFIR GIFVRFMNR CWCWDLEKV FPQYGDFKV 
      AENILHLVN NQGMVWMGM FFSPGLVVL NIFKLFVLM 
      YFRTWIVLT CNVPHEIIF RASRSNNFY YALLLMMLF 
      LCVLPVGYA IVQIPPSWN CGPFSNYQR IFHILTKTI 
      KRNVPQIVE RVLDYIASP AIVIPILLL LVLIIYYLV 
      SLTSALREA NNDLKFQLR RERTEERRK MFQIADRRR 
      KGKSGGSGE FSNTPFAKW RKLMSTMPS TGKSFDDTS 
      PRQETNESH QNKEDKVEI GKNKDFFSK FIKKALGKT 
      SSISENENR ENENLDEGT DTEQHESLP EDLVSIKDS 
      KPQPPSRLS ISSNSFDYQ KGLLQKQSS LKSVSESSN 
      KYNPVRDIG SVQQKKQLR QDSSSSNWS DNIPVITIS 
      KTESAEFLD VVPEAEKAP VTEKEIVKE TAPEAANEF 
      RFKPKIKCA LKKQSAEID EEVIRYFSK DLELKPEPD 
      AGPPSESSE DTARDSSLD TVLAVKSEE SAEERTDAP 
      KANPEVASP NESTTESSA EYNENMTSL
    CDS sequence
    • >TC032517 RA
      atgttaaca gcagtgccc gacgctcgc aaacaaatt 
      ctcaaggaa gagcgctac aactcgaca aactttcaa 
      actttgtgg gactttggg ggcgttttc aaatactcg 
      ccgttattc tacggctgg tacacgtac aaagaatca 
      aaaaagagc ggctatcgg atgcctttt gcttatttc 
      ataactggc ctggtggcc tatgcttat agcttcttt 
      gcaactttg agaaagatg gcggaaaac tctcgtatg 
      tctaagctt tcagaaaag gatgatgaa tgcattttc 
      tcgtggaaa ctcttcacg gcgtgggat tacatgatt 
      gggaacgcg gaaacggcg cataataga atagcctcg 
      attgtcgtc ggatttaag gaagctttg ctcgaggaa 
      gccgagaag aagagggag gctagaaat tggaagatc 
      acaggattt aggatactc gtgaatacg gtcgtgttg 
      gggctgcta ttcttctcg gcgtttgcc gtcattttg 
      gttgtgaaa agatctacg gaaccggaa gctaggagt 
      agtgtttgg aggagtaat gaaatcacc tttgttatg 
      acgtttatc tctatatct ttccctatg atatttgaa 
      gggttggga tttcttgaa caataccat cccagaaaa 
      caactacga ttacagctc gcaaggatc atggtgtta 
      aaccttctc aacttgtac tctttaata tttgccctg 
      ttcgataaa atcaacgat atgacacct gaactggaa 
      agcttcaag agaaattta acaagaaat aacacgagt 
      gtgttagct tattcagcg ggaatgcaa tgctacaat 
      agctgcaat tccgaaacc gatactctt aaagccttc 
      aatttaata gcggccgtt gctaccaat gtttcattt 
      acaatgtat ccagttatg caaaaagca gtgaaaaac 
      gcaactacg gagcctgat aaagaaacc atagacgcg 
      gaatttaca actggaatg tacacaaat ccgatggat 
      tttttagac aactacacg accgaattg cctggaaat 
      ttttcgagt gatgattca acaactttt aaaataagt 
      acaaatttg ccagacttt gtaacaaat agtttatct 
      acaagtgat ggtaccgaa gaaagtact tccgctagc 
      gccgacctg atactatct acagccaca ataaacgaa 
      acttctgca gataaacca aaactgcaa ggaagcgcc 
      gtcaaaaaa cgaaattgt ctcgaaata tgcgaaaat 
      ttcacagtt acaccttca ccgattata gatgaaagc 
      agtctaaac tatacgacc agacgtcga ttgagaaat 
      ctctgttgg gaaacaaat tttggccaa gaattaatc 
      aaactgacg gtcatggac ctcgtaatg acaatactg 
      tcgaccttg gctatggac tttatcaga ggcatattt 
      gtccgtttt atgaaccgc tgttggtgt tgggacttg 
      gaaaaggtg ttccctcaa tatggagac tttaaggtt 
      gccgaaaac atcctccac ttggtcaat aaccaagga 
      atggtttgg atgggaatg tttttctcg cccggtcta 
      gtcgtctta aacatcttc aaacttttc gttctgatg 
      tacttccgc acttggatt gtgctgact tgtaatgta 
      cctcacgaa atcatcttt cgagcttca agatctaac 
      aacttctac tacgccctt ttgctcatg atgctcttt 
      ttgtgcgtt ttgccagtg gggtacgca attgtccaa 
      ataccgccc tcttggaac tgtggccct ttctcgaat 
      tatcagaga atttttcat attttaacc aagactatt 
      aaaagaaac gtgcctcaa atcgtagag cgggttttg 
      gattacata gcgtcacct gcaattgta ataccaata 
      cttctgtta ttggtgtta attatttac tatctggtg 
      tctttaaca agcgctctt agagaggca aacaatgat 
      ttgaagttc caactcagg agagaacgt acggaagaa 
      cgtcgaaaa atgttccaa attgcagat cggaggaga 
      aaaggaaaa tctggtggt tcgggagaa ttctccaat 
      actcctttt gccaaatgg aggaaatta atgtcaaca 
      atgccaagc actggcaaa agtttcgat gatacttca 
      ccgaggcaa gaaaccaac gaatctcat caaaacaaa 
      gaagataaa gttgaaatt ggaaaaaat aaagatttt 
      ttttcaaaa ttcattaaa aaagcgtta ggcaagact 
      tcttcaata tcagaaaac gaaaatcgt gaaaatgaa 
      aatctggac gaagggacc gacacagaa caacatgaa 
      tcattacct gaagattta gtcagcatt aaagacagc 
      aaacctcaa ccaccttca agattatca atttcttcc 
      aacagtttt gactaccaa aaaggtttg ctacaaaag 
      cagagcagt ctgaagagt gtgagtgaa tctagcaat 
      aaatacaac ccggtcagg gatattgga agtgtccaa 
      caaaagaaa caattacgt caggactca tcttcgtcc 
      aattggtct gataatatc cctgtcatc acgatcagc 
      aaaactgaa agcgccgaa tttcttgat gtggtacca 
      gaagcggaa aaagcacca gtgacggaa aaagaaatc 
      gtgaaggaa acggcaccg gaggcggct aatgaattt 
      cgatttaaa cccaaaatt aaatgtgcc ttgaagaaa 
      caatctgca gaaattgac gaagaagtg attcgttac 
      tttagtaag gatttggag ctgaaaccg gagccggac 
      gctggacct ccatctgaa tcttccgaa gatacggca 
      cgtgacagt agccttgac acagttttg gcggtaaaa 
      agcgaagaa tctgcggaa gaaagaacc gatgctccg 
      aaagctaac cctgaggtt gcatcacct aatgagagc 
      acaactgaa tccagtgct gagtacaat gagaacatg 
      acttctttg taa
    mRNA sequence
    • >TC032517 RA
      aaatttccg taaatcctt aaacaattt ttttatcgc 
      tcgtgcaat aaatacgat ttcagatgt taacagcag 
      tgcccgacg ctcgcaaac aaattctca aggaagagc 
      gctacaact cgacaaact ttcaaactt tgtgggact 
      ttgggggcg ttttcaaat actcgccgt tattctacg 
      gctggtaca cgtacaaag aatcaaaaa agagcggct 
      atcggatgc cttttgctt atttcataa ctggcctgg 
      tggcctatg cttatagct tctttgcaa ctttgagaa 
      agatggcgg aaaactctc gtatgtcta agctttcag 
      aaaaggatg atgaatgca ttttctcgt ggaaactct 
      tcacggcgt gggattaca tgattggga acgcggaaa 
      cggcgcata atagaatag cctcgattg tcgtcggat 
      ttaaggaag ctttgctcg aggaagccg agaagaaga 
      gggaggcta gaaattgga agatcacag gatttagga 
      tactcgtga atacggtcg tgttggggc tgctattct 
      tctcggcgt ttgccgtca ttttggttg tgaaaagat 
      ctacggaac cggaagcta ggagtagtg tttggagga 
      gtaatgaaa tcacctttg ttatgacgt ttatctcta 
      tatctttcc ctatgatat ttgaagggt tgggatttc 
      ttgaacaat accatccca gaaaacaac tacgattac 
      agctcgcaa ggatcatgg tgttaaacc ttctcaact 
      tgtactctt taatatttg ccctgttcg ataaaatca 
      acgatatga cacctgaac tggaaagct tcaagagaa 
      atttaacaa gaaataaca cgagtgtgt tagcttatt 
      cagcgggaa tgcaatgct acaatagct gcaattccg 
      aaaccgata ctcttaaag ccttcaatt taatagcgg 
      ccgttgcta ccaatgttt catttacaa tgtatccag 
      ttatgcaaa aagcagtga aaaacgcaa ctacggagc 
      ctgataaag aaaccatag acgcggaat ttacaactg 
      gaatgtaca caaatccga tggattttt tagacaact 
      acacgaccg aattgcctg gaaattttt cgagtgatg 
      attcaacaa cttttaaaa taagtacaa atttgccag 
      actttgtaa caaatagtt tatctacaa gtgatggta 
      ccgaagaaa gtacttccg ctagcgccg acctgatac 
      tatctacag ccacaataa acgaaactt ctgcagata 
      aaccaaaac tgcaaggaa gcgccgtca aaaaacgaa 
      attgtctcg aaatatgcg aaaatttca cagttacac 
      cttcaccga ttatagatg aaagcagtc taaactata 
      cgaccagac gtcgattga gaaatctct gttgggaaa 
      caaattttg gccaagaat taatcaaac tgacggtca 
      tggacctcg taatgacaa tactgtcga ccttggcta 
      tggacttta tcagaggca tatttgtcc gttttatga 
      accgctgtt ggtgttggg acttggaaa aggtgttcc 
      ctcaatatg gagacttta aggttgccg aaaacatcc 
      tccacttgg tcaataacc aaggaatgg tttggatgg 
      gaatgtttt tctcgcccg gtctagtcg tcttaaaca 
      tcttcaaac ttttcgttc tgatgtact tccgcactt 
      ggattgtgc tgacttgta atgtacctc acgaaatca 
      tctttcgag cttcaagat ctaacaact tctactacg 
      cccttttgc tcatgatgc tctttttgt gcgttttgc 
      cagtggggt acgcaattg tccaaatac cgccctctt 
      ggaactgtg gccctttct cgaattatc agagaattt 
      ttcatattt taaccaaga ctattaaaa gaaacgtgc 
      ctcaaatcg tagagcggg ttttggatt acatagcgt 
      cacctgcaa ttgtaatac caatacttc tgttattgg 
      tgttaatta tttactatc tggtgtctt taacaagcg 
      ctcttagag aggcaaaca atgatttga agttccaac 
      tcaggagag aacgtacgg aagaacgtc gaaaaatgt 
      tccaaattg cagatcgga ggagaaaag gaaaatctg 
      gtggttcgg gagaattct ccaatactc cttttgcca 
      aatggagga aattaatgt caacaatgc caagcactg 
      gcaaaagtt tcgatgata cttcaccga ggcaagaaa 
      ccaacgaat ctcatcaaa acaaagaag ataaagttg 
      aaattggaa aaaataaag atttttttt caaaattca 
      ttaaaaaag cgttaggca agacttctt caatatcag 
      aaaacgaaa atcgtgaaa atgaaaatc tggacgaag 
      ggaccgaca cagaacaac atgaatcat tacctgaag 
      atttagtca gcattaaag acagcaaac ctcaaccac 
      cttcaagat tatcaattt cttccaaca gttttgact 
      accaaaaag gtttgctac aaaagcaga gcagtctga 
      agagtgtga gtgaatcta gcaataaat acaacccgg 
      tcagggata ttggaagtg tccaacaaa agaaacaat 
      tacgtcagg actcatctt cgtccaatt ggtctgata 
      atatccctg tcatcacga tcagcaaaa ctgaaagcg 
      ccgaatttc ttgatgtgg taccagaag cggaaaaag 
      caccagtga cggaaaaag aaatcgtga aggaaacgg 
      caccggagg cggctaatg aatttcgat ttaaaccca 
      aaattaaat gtgccttga agaaacaat ctgcagaaa 
      ttgacgaag aagtgattc gttacttta gtaaggatt 
      tggagctga aaccggagc cggacgctg gacctccat 
      ctgaatctt ccgaagata cggcacgtg acagtagcc 
      ttgacacag ttttggcgg taaaaagcg aagaatctg 
      cggaagaaa gaaccgatg ctccgaaag ctaaccctg 
      aggttgcat cacctaatg agagcacaa ctgaatcca 
      gtgctgagt acaatgaga acatgactt ctttgtaaa 
      aatattcct tgctgtcgt aaaaacatg aaatatgaa 
      tatctaact gtgttttat tttaacgag gccaactaa 
      tttggcttt tatcgatgt ttcaacatt ttattcaaa 
      caaaccacc gaaacgata agtaaaacg aaatttttg 
      acctccaac ctctagtta tcacgcaag ttgatgaac 
      ttttaacta attttaaat ttcgtttgt atttgtttt 
      cctgtaacg atgaagctg atctaattt cggtgttta 
      tggtgttat tatgtcatt agtggtgtc aaatgagat 
      cattaacac atgcaaatt gtgactcac caacaatta 
      atcaataaa gccattaaa tacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY