Status
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Gene information
General information
TC032508 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14337503..14359446
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032508
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09790

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032508 RA
      MAEGSYMDN ISTFVALPV DTLENNILQ KPEQFRIIK 
      NAIIKKLSD GSVCIIDAN EVNEGLPQE IEVTYLPPV 
      DVTPVANAT PDKPTVTVN KETSPKQTY KCSKCKEVF 
      EKLVLYRKH MMWHRSIKK FKCEKCSVG YNVETNLKI 
      HMAMHSEGK PTCPICNIS FQRLASLKS HLMLHQVEE 
      LCTCEECGA EFEKEEELE KHFHSHLEN TAKENNNLV 
      CTYCSVQLD DQKSYKEHI SHHVKVKKM VLSGKKSRK 
      THGNKEYPH VCNICSKSF SRVSLLERH SRIHSGEKP 
      FLCQYCFRG FNQKGTLQI HYSKHTGHK PFQCTLCSA 
      KFSQKGNLR VHVEKTHTA PLPGVKMYK CSHCTCIFK 
      KIASLNVHI TKFHSNGED STSISSVMN QLKELEQVA 
      MPSSHSETV QENERVDDP NTSFVRLAE SFLDGTVRR 
      YLVKQKKVN DVRWYICNY CNKEFKKPS DLIRHTRVH 
      TREKPFVCN KCGASFSLK TSLFSHVKT HLGNNNYFC 
      SICMKKFSS ARSVNLHLR RHSEKPQYK CLSCNKSFS 
      SIQKAKLHY KEAHSGRSE KDSEIILKQ PLVNTPDGL 
      LPVPPPKSV HSTNSKQIQ CPICCVRFS KVPNLKRHM 
      LLHNGGKKH KCGQCMKSY YTAHALKEH VLCHSGVKN 
      FGCKVCGKK YTTASLLKR HTLSHSTNK PYVCPYCSK 
      NFKSVMLCR KHMNVHKKD LQITVQNIS NETKNLDID 
      KPKLLLIPD SISNSGLLT NNQDPLETT NLQLITQPN 
      VNEIVNPTD QQFYEITPN SIQIITNSD KDLVQDPNQ 
      IQTIFINCD NLPLGDNLY STNTNSVLF DPMNENTFN 
      INALNGDFS TQNTETFND GQTNSTSTD KVSNDNLLT 
      TNIPLYPQS ELNLLFNLD TEFVSNNCD LPDININEV 
      PDLNVEPAK ENDEAKNRM TCDKCSKVF TNKKTFLKH 
      VNSHKDRQK VAGFHQCSF CEKSFKKHS DLIRHIRTH 
      TGEKPFACD KCEKKFSLK STLLSHQRT HNPISHKQF 
      NCVVCNSFF TSKSSLKVH MSVHTGDKS YKCSFCSAK 
      FRTAAHRKS HENNYHVNP SKKKTKNKV TNLLESVAM 
      DVLGQTEQT EQSEQVGGL ENVLVVQEP LNLLDDSGQ 
      NALTESTQL EPLLLQQLQ SNFLLQYNL VSSSLIQLD 
      DGTLVNFKT VNTDQDLLQ ETVKPPSEP TTPAEKLRN 
      LECDICHKK YASKDVLRK HKKIHGVDK KFRCTKCEK 
      GFDTEEDLE KHKRIHEGY RPYSCFYCA NSFSKEHSL 
      ATHLKRIHN VSLKDNLLL CDLK
    CDS sequence
    • >TC032508 RA
      atggctgaa ggaagttat atggataac atttcgact 
      tttgtcgcc ctaccagtg gatactttg gaaaataac 
      atcctccaa aaacctgaa caatttcgc ataatcaaa 
      aatgcaatc attaaaaaa ttatctgat ggttcagtt 
      tgtattata gacgcaaat gaggttaat gaaggattg 
      ccacaagaa atcgaagta acttatttg ccccctgtt 
      gacgttacg ccggtggct aacgccact ccagacaag 
      ccaacagta actgtaaat aaggaaact tcacctaaa 
      caaacatac aagtgttcc aaatgcaag gaagtgttc 
      gaaaaactt gtcttatat cgaaaacac atgatgtgg 
      caccgaagt ataaaaaaa ttcaaatgt gagaaatgt 
      tcagtaggt tataatgtg gaaacaaat ttaaaaatt 
      catatggca atgcatagt gaagggaag cctacttgt 
      ccaatttgc aatatttcg tttcaaaga ttggcaagt 
      ttaaaatct catttaatg ttgcatcaa gttgaggaa 
      ttgtgcact tgtgaagaa tgtggagct gaatttgaa 
      aaagaagaa gaattagaa aaacatttt catagtcat 
      ttggaaaat accgccaag gaaaataat aatttggtt 
      tgtacttac tgtagtgtt caattggat gatcaaaaa 
      agttataaa gagcacatt tcacatcat gtcaaggtg 
      aagaagatg gttttatcg ggaaaaaaa tcccgcaaa 
      acccacggc aacaaagaa taccctcac gtgtgtaac 
      atttgttcg aaaagtttt tcgcgagtt tcgctcctg 
      gaaagacac tcacgtatc cacagtgga gaaaaacct 
      ttcctttgt caatactgt tttcgtggt ttcaaccaa 
      aaaggcact ctacaaatt cattacagt aaacatacg 
      gggcataaa ccattccag tgcacttta tgttcggcg 
      aaattcagc caaaaaggc aatcttcgg gtacatgtt 
      gaaaaaaca cacacagcg cctttacca ggcgttaaa 
      atgtataaa tgttcacac tgcacttgc atttttaaa 
      aaaattgcc tctttgaac gtccatatc acgaagttt 
      cattcaaac ggcgaagac tccacgagt atttcgagt 
      gtaatgaat cagttgaaa gaactggaa caagtagca 
      atgccttct tcacacagt gagactgta caggaaaat 
      gaacgtgtt gacgatccc aatacaagt tttgtcaga 
      ttggctgaa tcgttcctt gatggaaca gtcagacga 
      tatttggtg aagcagaag aaagtgaac gatgtcaga 
      tggtacatt tgtaactac tgtaacaaa gagttcaaa 
      aaaccgtcg gatcttatc agacataca agggttcat 
      acgagggaa aaaccattt gtgtgtaac aaatgtggt 
      gcctcgttt tccctaaaa acgagcctt ttttcacac 
      gttaaaacc caccttggg aataataac tacttttgt 
      tcgatttgt atgaaaaaa ttttcgtcg gctcgttcg 
      gttaacttg cacttgagg agacactcg gagaagcca 
      caatacaaa tgtttatct tgcaacaaa agtttcagt 
      tcgattcaa aaagccaaa ctccactat aaagaagct 
      cacagtgga cgatctgaa aaagactcg gaaataatt 
      ctgaagcaa cctttagtt aacacacct gatggttta 
      ttaccagtg ccaccacca aaatccgta cattcaaca 
      aattcaaaa caaatccaa tgtccgatt tgttgtgta 
      cgatttagt aaagtaccg aatttaaaa cgtcatatg 
      ctgctacat aacggcgga aaaaagcac aagtgtggt 
      caatgtatg aagtcgtat tatacagct catgctttg 
      aaggaacat gtgttgtgt cattctggt gttaaaaat 
      ttcggatgt aaagtgtgt gggaaaaaa tatacaacg 
      gcttcgtta ttgaagaga cacactctc agtcacagt 
      acgaacaag ccttatgtt tgtccatac tgtagcaag 
      aattttaaa agtgttatg ttgtgtcgc aaacatatg 
      aacgtacac aagaaggat ctacagatc actgttcaa 
      aacatatct aatgaaacc aaaaatttg gatattgat 
      aaaccaaag ctacttctt atacctgat tcgatatcc 
      aactctggt ttattaaca aataaccaa gatcctcta 
      gaaaccact aatctccag ttaattact caaccaaat 
      gtgaacgaa attgtgaat ccaactgac caacaattt 
      tatgaaata acaccaaac tctatccaa ataattaca 
      aacagtgac aaggatctt gtccaagat cccaaccaa 
      atacagact attttcatc aattgcgac aatttacca 
      ttgggcgat aacttgtat tcaacgaac acaaattca 
      gttttgttt gatccaatg aatgaaaac actttcaat 
      attaatgcc ttgaatgga gatttttca acacaaaac 
      accgaaaca tttaatgat ggacaaacc aatagcact 
      tcaaccgat aaagtttcg aatgataat ctcctaact 
      accaacata ccattgtat ccacaatct gaactcaat 
      ctactgttc aatctagac actgaattc gtttcaaac 
      aattgcgat ttacctgat attaatata aacgaagtg 
      cctgactta aacgtcgaa cctgcgaaa gaaaacgac 
      gaagcgaaa aatcgaatg acttgcgat aaatgtagc 
      aaagtgttc actaacaag aaaactttt ttgaaacat 
      gttaacagc cacaaagac cggcaaaaa gtcgccggt 
      ttccaccag tgcagtttc tgtgagaaa agcttcaag 
      aaacactct gatttgata cgccacatt cgcacacac 
      acgggggaa aaaccgttt gcttgtgac aagtgcgag 
      aaaaagttt tcactaaaa tcaactcta ttgtcgcat 
      cagcgcact cataatccg atcagtcac aaacagttt 
      aattgtgtc gtttgtaac tcatttttc acgtcaaag 
      tctagcctc aaggtgcac atgagtgtg catacaggt 
      gataaaagc tataagtgt agtttttgt tcggctaaa 
      ttccgaacg gcggctcat cgaaaatcg cacgagaat 
      aattatcac gtgaatccg tcaaagaag aaaacgaaa 
      aacaaagtt acgaatttg ctcgaatcc gttgcaatg 
      gatgtgttg ggacaaacg gagcaaact gagcaaagt 
      gaacaagtt gggggtttg gaaaatgtt cttgtggtg 
      caggagccg ttaaatctt ctggatgat tccggtcag 
      aacgcatta accgaatcg acacaactc gaaccgtta 
      cttttgcaa cagttgcaa tcgaatttt ttgttgcaa 
      tataattta gtttcgtcg tctttgatt caactagac 
      gatgggaca cttgttaat tttaaaacg gttaacacc 
      gatcaagac ttattgcaa gaaacggtt aaaccgcca 
      agtgaacca acaacacca gccgaaaaa ctgaggaat 
      ttggaatgt gacatttgt cacaagaaa tacgcgtct 
      aaagatgtt ttaaggaaa cataaaaaa atccacggt 
      gtggataag aagtttagg tgtacgaag tgtgaaaag 
      ggatttgat acggaggag gatttggag aagcataaa 
      aggatacat gaggggtat aggccatat tcgtgtttc 
      tattgcgct aatagtttt tcaaaagag catagtttg 
      gcaacacat ttgaaaagg attcacaat gtttcgctc 
      aaggataat cttttattg tgtgatctt aaataa
    mRNA sequence
    • >TC032508 RA
      agttaacct acaaataac cgagagtta ttttttgat 
      aatttcgta attatatgt tgaaatggc tgaaggaag 
      ttatatgga taacatttc gacttttgt cgccctacc 
      agtggatac tttggaaaa taacatcct ccaaaaacc 
      tgaacaatt tcgcataat caaaaatgc aatcattaa 
      aaaattatc tgatggttc agtttgtat tatagacgc 
      aaatgaggt taatgaagg attgccaca agaaatcga 
      agtaactta tttgccccc tgttgacgt tacgccggt 
      ggctaacgc cactccaga caagccaac agtaactgt 
      aaataagga aacttcacc taaacaaac atacaagtg 
      ttccaaatg caaggaagt gttcgaaaa acttgtctt 
      atatcgaaa acacatgat gtggcaccg aagtataaa 
      aaaattcaa atgtgagaa atgttcagt aggttataa 
      tgtggaaac aaatttaaa aattcatat ggcaatgca 
      tagtgaagg gaagcctac ttgtccaat ttgcaatat 
      ttcgtttca aagattggc aagtttaaa atctcattt 
      aatgttgca tcaagttga ggaattgtg cacttgtga 
      agaatgtgg agctgaatt tgaaaaaga agaagaatt 
      agaaaaaca ttttcatag tcatttgga aaataccgc 
      caaggaaaa taataattt ggtttgtac ttactgtag 
      tgttcaatt ggatgatca aaaaagtta taaagagca 
      catttcaca tcatgtcaa ggtgaagaa gatggtttt 
      atcgggaaa aaaatcccg caaaaccca cggcaacaa 
      agaataccc tcacgtgtg taacatttg ttcgaaaag 
      tttttcgcg agtttcgct cctggaaag acactcacg 
      tatccacag tggagaaaa acctttcct ttgtcaata 
      ctgttttcg tggtttcaa ccaaaaagg cactctaca 
      aattcatta cagtaaaca tacggggca taaaccatt 
      ccagtgcac tttatgttc ggcgaaatt cagccaaaa 
      aggcaatct tcgggtaca tgttgaaaa aacacacac 
      agcgccttt accaggcgt taaaatgta taaatgttc 
      acactgcac ttgcatttt taaaaaaat tgcctcttt 
      gaacgtcca tatcacgaa gtttcattc aaacggcga 
      agactccac gagtatttc gagtgtaat gaatcagtt 
      gaaagaact ggaacaagt agcaatgcc ttcttcaca 
      cagtgagac tgtacagga aaatgaacg tgttgacga 
      tcccaatac aagttttgt cagattggc tgaatcgtt 
      ccttgatgg aacagtcag acgatattt ggtgaagca 
      gaagaaagt gaacgatgt cagatggta catttgtaa 
      ctactgtaa caaagagtt caaaaaacc gtcggatct 
      tatcagaca tacaagggt tcatacgag ggaaaaacc 
      atttgtgtg taacaaatg tggtgcctc gttttccct 
      aaaaacgag ccttttttc acacgttaa aacccacct 
      tgggaataa taactactt ttgttcgat ttgtatgaa 
      aaaattttc gtcggctcg ttcggttaa cttgcactt 
      gaggagaca ctcggagaa gccacaata caaatgttt 
      atcttgcaa caaaagttt cagttcgat tcaaaaagc 
      caaactcca ctataaaga agctcacag tggacgatc 
      tgaaaaaga ctcggaaat aattctgaa gcaaccttt 
      agttaacac acctgatgg tttattacc agtgccacc 
      accaaaatc cgtacattc aacaaattc aaaacaaat 
      ccaatgtcc gatttgttg tgtacgatt tagtaaagt 
      accgaattt aaaacgtca tatgctgct acataacgg 
      cggaaaaaa gcacaagtg tggtcaatg tatgaagtc 
      gtattatac agctcatgc tttgaagga acatgtgtt 
      gtgtcattc tggtgttaa aaatttcgg atgtaaagt 
      gtgtgggaa aaaatatac aacggcttc gttattgaa 
      gagacacac tctcagtca cagtacgaa caagcctta 
      tgtttgtcc atactgtag caagaattt taaaagtgt 
      tatgttgtg tcgcaaaca tatgaacgt acacaagaa 
      ggatctaca gatcactgt tcaaaacat atctaatga 
      aaccaaaaa tttggatat tgataaacc aaagctact 
      tcttatacc tgattcgat atccaactc tggtttatt 
      aacaaataa ccaagatcc tctagaaac cactaatct 
      ccagttaat tactcaacc aaatgtgaa cgaaattgt 
      gaatccaac tgaccaaca attttatga aataacacc 
      aaactctat ccaaataat tacaaacag tgacaagga 
      tcttgtcca agatcccaa ccaaataca gactatttt 
      catcaattg cgacaattt accattggg cgataactt 
      gtattcaac gaacacaaa ttcagtttt gtttgatcc 
      aatgaatga aaacacttt caatattaa tgccttgaa 
      tggagattt ttcaacaca aaacaccga aacatttaa 
      tgatggaca aaccaatag cacttcaac cgataaagt 
      ttcgaatga taatctcct aactaccaa cataccatt 
      gtatccaca atctgaact caatctact gttcaatct 
      agacactga attcgtttc aaacaattg cgatttacc 
      tgatattaa tataaacga agtgcctga cttaaacgt 
      cgaacctgc gaaagaaaa cgacgaagc gaaaaatcg 
      aatgacttg cgataaatg tagcaaagt gttcactaa 
      caagaaaac ttttttgaa acatgttaa cagccacaa 
      agaccggca aaaagtcgc cggtttcca ccagtgcag 
      tttctgtga gaaaagctt caagaaaca ctctgattt 
      gatacgcca cattcgcac acacacggg ggaaaaacc 
      gtttgcttg tgacaagtg cgagaaaaa gttttcact 
      aaaatcaac tctattgtc gcatcagcg cactcataa 
      tccgatcag tcacaaaca gtttaattg tgtcgtttg 
      taactcatt tttcacgtc aaagtctag cctcaaggt 
      gcacatgag tgtgcatac aggtgataa aagctataa 
      gtgtagttt ttgttcggc taaattccg aacggcggc 
      tcatcgaaa atcgcacga gaataatta tcacgtgaa 
      tccgtcaaa gaagaaaac gaaaaacaa agttacgaa 
      tttgctcga atccgttgc aatggatgt gttgggaca 
      aacggagca aactgagca aagtgaaca agttggggg 
      tttggaaaa tgttcttgt ggtgcagga gccgttaaa 
      tcttctgga tgattccgg tcagaacgc attaaccga 
      atcgacaca actcgaacc gttactttt gcaacagtt 
      gcaatcgaa ttttttgtt gcaatataa tttagtttc 
      gtcgtcttt gattcaact agacgatgg gacacttgt 
      taattttaa aacggttaa caccgatca agacttatt 
      gcaagaaac ggttaaacc gccaagtga accaacaac 
      accagccga aaaactgag gaatttgga atgtgacat 
      ttgtcacaa gaaatacgc gtctaaaga tgttttaag 
      gaaacataa aaaaatcca cggtgtgga taagaagtt 
      taggtgtac gaagtgtga aaagggatt tgatacgga 
      ggaggattt ggagaagca taaaaggat acatgaggg 
      gtataggcc atattcgtg tttctattg cgctaatag 
      tttttcaaa agagcatag tttggcaac acatttgaa 
      aaggattca caatgtttc gctcaagga taatctttt 
      attgtgtga tcttaaata aaatgaatt atgaagagt 
      ttaggtcat tggaagtgt acataaaat tttatttag 
      tattaataa tttataaat acatatatg ttttgtaa
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