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Gene information
General information
TC003691 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12771615..12774931
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003691
    iBeetle screen iB_09787

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003691 RA
      MKRKGRKSK KSETETANG VSDQKDQNG DASDRDEHE 
      SEPEEKKRK SNKRKSSSK KVEDESEEE PQYEVEQVL 
      DEKVIRGVH HYLIRWKGY EPESDTWEP ESTLNCAEL 
      IADFKARQK KKGKSKVGR KKSKDSGKN NSTEETWDE 
      NENFEVDRI LDVYFKRNG QREFLVSWK GYPNSQNSW 
      EPEENMDCK DLIKKFMSK VEKAKEAEH RELRVNRAH 
      TDRLTLLSD GHLGRRLSK RNLGKQRVH YHEAE
    CDS sequence
    • >TC003691 RA
      atgaaacgt aaaggacgg aaaagcaaa aagtccgag 
      acggaaacc gccaacggc gtgagtgac cagaaagac 
      caaaacggt gacgcttcg gatcgggac gagcacgaa 
      tccgaaccg gaggaaaaa aaacgcaag tctaacaaa 
      cgtaaaagt tcttcgaag aaagtggaa gatgaatcg 
      gaagaggaa ccgcaatat gaggttgag caagtcttg 
      gacgagaag gtcatcaga ggggtacac cattacctc 
      atcagatgg aagggctac gaaccggaa tcggacacc 
      tgggagccc gaatcgacc ctcaattgt gcagaactg 
      atcgccgat ttcaaagcc aggcagaaa aagaaaggc 
      aaatcgaaa gtaggaagg aagaaatcg aaagacagc 
      ggcaaaaat aactccact gaagagaca tgggatgaa 
      aatgaaaat tttgaggtt gatcgcatt ttggacgtc 
      tactttaaa agaaacggc caacgtgaa ttcctagtg 
      tcatggaag ggttacccc aactcacaa aacagttgg 
      gaacctgaa gagaacatg gattgtaaa gatttaatt 
      aaaaaattc atgagtaaa gtggaaaag gctaaagaa 
      gccgaacac agagagcta cgtgtcaat agggcgcac 
      acagatcga ttgactctc ctttcagac ggccatttg 
      ggacgtcgt ttgagcaaa agaaatcta ggaaaacaa 
      agggttcac taccacgaa gctgaataa
    mRNA sequence
    • >TC003691 RA
      ttttttatt aggttggtg ttacagttg gtcacactg 
      tagcacgat aatcgctcc cgtggcgtc ttttataaa 
      agataaaca aattctttc acaacaaat ttaaatttt 
      cgtgtcgca gaaatgaaa cgtaaagga cggaaaagc 
      aaaaagtcc gagacggaa accgccaac ggcgtgagt 
      gaccagaaa gaccaaaac ggtgacgct tcggatcgg 
      gacgagcac gaatccgaa ccggaggaa aaaaaacgc 
      aagtctaac aaacgtaaa agttcttcg aagaaagtg 
      gaagatgaa tcggaagag gaaccgcaa tatgaggtt 
      gagcaagtc ttggacgag aaggtcatc agaggggta 
      caccattac ctcatcaga tggaagggc tacgaaccg 
      gaatcggac acctgggag cccgaatcg accctcaat 
      tgtgcagaa ctgatcgcc gatttcaaa gccaggcag 
      aaaaagaaa ggcaaatcg aaagtagga aggaagaaa 
      tcgaaagac agcggcaaa aataactcc actgaagag 
      acatgggat gaaaatgaa aattttgag gttgatcgc 
      attttggac gtctacttt aaaagaaac ggccaacgt 
      gaattccta gtgtcatgg aagggttac cccaactca 
      caaaacagt tgggaacct gaagagaac atggattgt 
      aaagattta attaaaaaa ttcatgagt aaagtggaa 
      aaggctaaa gaagccgaa cacagagag ctacgtgtc 
      aatagggcg cacacagat cgattgact ctcctttca 
      gacggccat ttgggacgt cgtttgagc aaaagaaat 
      ctaggaaaa caaagggtt cactaccac gaagctgaa 
      taaaaaacc atgtagtac aatatcatt cggaaatgt 
      tcatgtgtt taagagaaa ttttctttt ctatcgata 
      actgtactt ttcatttgt tgaaacaag gttttttta 
      taattataa gcatatact tacataata tatttaggg 
      tagttctaa taaaaatgt gcaaacaca aaaaagttg 
      tgctcatga aatttatgt ttattaatt acagatgca 
      gaatctttg tttatttta taggtcaga tatttaagt 
      atttcgtgt ttatgtaaa ataaatttt tcacaaaga 
      t
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