Status
Please wait ...
Gene information
General information
TC000041 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10691922..10699899
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000041
    iBeetle screen iB_09783

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000041 RA
      MEEKPAVST APAAPAGAA PPEPAQTAP VQWAYPMYN 
      YDMSNYYHQ FYLMNSYPA YQSAIYHQV QQQMLENKP 
      STTSADANK FVPPLPPGT PSSLPTPHI SKPPLLNTP 
      KQFTANNNN PIRFNLPPG KRQNGNLLL QQNNSLTSG 
      AAKKKRKRN RNNQNQNLQ NQNFFPLPP LPPPEQNVP 
      KPAPPPETM PPEPPLPPL PDTSKPPPP PAEKPPPTS 
      NNPTDEWPP SLKDYVNRC YAKCKTNID KNQVEIILK 
      GKITHAYQM GQLHTKDWS NEPLPNIHS ERPTLVPKT 
      VPGSLAQFQ NTTKKGLSP AMGARLGAR ASTLRGTSR 
      SSSRSRSRS PARKKSRSR SRSPRRHRS SSSSSSVSS 
      DENFKPLVK PNNKNKIKS KLANRLGPT KNNKFTNKQ 
      LKKQKMKEK KAHLLSSFG SELEENSEL LLQRAARFN 
      NNKQKETNG LINNKNVFV NDKYEDNIA DFDWTGFHI 
      VGTCQDLEK SFLRLTKAP EACEVRPVE VLKLSLQNV 
      KTRWKQKPD YYYTCDQLK SIRQDLTVQ GVRNEFTVE 
      VYETHARIA LEKGDHEEF NQCQTQLKM LYSEVGGGN 
      KNEFTAYRI LYYIFTKNT LDIMTIMKS LSKEEKSDK 
      CIDFALKLR TAWSLGNFH RFFQLYLQA PLMAGFLVD 
      WFIERERKL YLKCIIKR
    • >TC000041 RB
      MEEKPAVST APAAPAGAA PPEPAQTAP VQWAYPMYN 
      YDMSNYYHQ FYLMNSYPA YQSAIYHQV QQQMLENKP 
      STTSADANK FVPPLPPGT PSSLPTPHI SKPPLLNTP 
      KQFTANNNN PIRFNLPPG KRQNGNLLL QQNNSLTSG 
      AAKKKRKRN RNNQNQNLQ NQNFFPLPP LPPPEQNVP 
      KPAPPPETM PPEPPLPPL PDTSKPPPP PAEKPPPTS 
      NNPTDEWPP SLKDYVNRC YAKCKTNID KNQVEIILK 
      GKITHAYQM GQLHTKDWS NEPLPNIHS ERPTLVPKT 
      VPGSLAQFQ NTTKKGLSP AMGARLGAR ASTLRGTSR 
      SSSRSRSRS PARKKSRSR SRSPRRHRS SSSSSSVSS 
      DENFKPLVK PNNKNKIKS KLANRLGPT KNNKFTNKQ 
      LKKQKMKEK KAHLLSSFG SELEENSEL LLQRAARFN 
      NNKQKETNG LINNKNVFV NDKYEDNIA DFDWTGFHI 
      VGTCQDLEK SFLRLTKAP EACEVRPVE VLKLSLQNV 
      KTRWKQKPD YYYTCDQLK SIRQDLTVQ GVRNEFTVE 
      VYETHARIA LEKGDHEEF NQCQTQLKM LYSEVGGGN 
      KNEFTAYRI LYYIFTKNT LDIMTIMKS LSKEEKSDK 
      CIDFALKLR TAWSLGNFH RFFQLYLQA PLMAGFLVD 
      WFIERERKL YLKCIIKSY RQNISVNFI QQELAFPAL 
      EETLKFLEP FSLSFTDLS KSHIDCKTS MASLPNI
    CDS sequence
    • >TC000041 RA
      atggaggag aaacctgct gtctccact gctcctgca 
      gcacccgca ggagcagcg ccccctgaa cctgctcaa 
      acagcccca gtacaatgg gcttacccc atgtacaat 
      tacgacatg agcaattac taccaccaa ttttaccta 
      atgaacagt taccccgcc taccaatcg gcaatttac 
      caccaggtg caacaacaa atgctcgaa aacaaaccg 
      tcaacaaca tcagcggac gctaataaa ttcgtgcct 
      cctctccca ccaggcact ccatcatca ctcccaact 
      ccacacatc tcaaaacca cctcttctg aatactccc 
      aaacaattc accgccaac aacaacaac cccattagg 
      ttcaatctg cctcctggc aaacgccaa aacggcaac 
      ctcctcctc caacagaac aactccctg acaagtggc 
      gccgccaaa aagaaacgc aaacgtaac cgcaacaac 
      caaaaccaa aatttgcag aaccagaac tttttccca 
      cttcctcca ctcccgcca cccgaacaa aacgtaccc 
      aaaccggcg ccgcccccg gaaaccatg ccgccggaa 
      ccgccactg cccccgctt ccggacaca agcaagccg 
      ccgccgcct ccagcggaa aaaccgccc ccgacttcg 
      aataaccca acggatgaa tggccacca agtctcaaa 
      gactacgtg aatcgatgc tatgccaag tgcaagacg 
      aacattgat aagaatcaa gttgagatt attcttaag 
      gggaaaata acgcacgcg tatcagatg ggacagttg 
      catacgaaa gactggagc aacgaaccg ctcccgaat 
      atacacagt gaaagacca acactagta cccaagaca 
      gtacccgga tcattagca cagttccag aataccaca 
      aaaaaggga ctatcacca gcgatggga gcgcgttta 
      ggggcgaga gcgtccacg ctgcgaggt accagcagg 
      tcgtcttct aggtctagg tccaggtcg ccggcgagg 
      aagaagtcc aggtcgagg tccaggagc ccccgcagg 
      catcggtct tcaagcagc agttcgagc gtgagtagc 
      gacgagaac ttcaagcca ttagtgaag cccaacaac 
      aagaacaaa atcaaaagc aaactggcc aatagatta 
      ggcccaaca aagaacaac aaatttaca aacaaacaa 
      ctgaaaaaa cagaaaatg aaagagaaa aaagcacat 
      ttgttatca tcgtttggt tccgaatta gaagagaat 
      tccgaattg ttgcttcaa agagccgca cgtttcaat 
      aacaataaa cagaaagaa acaaatgga ttaataaat 
      aataaaaac gttttcgtg aatgataag tatgaggac 
      aatatcgca gactttgac tggacagga tttcacata 
      gtgggcacg tgtcaagac ttggaaaag tccttcctt 
      cgactgact aaagccccc gaagcatgt gaagtacgt 
      ccagttgaa gttctcaaa ttgtcatta cagaatgtc 
      aagacgaga tggaaacaa aaaccggat tattactac 
      acatgtgat caacttaaa tcaatcaga caagatctc 
      acagtacaa ggagtacgg aacgagttc acggttgaa 
      gtttacgaa actcacgcg cgcatcgct ttagaaaaa 
      ggcgatcac gaagagttc aatcagtgt caaacgcaa 
      ctcaaaatg ttatattcg gaagtcggc ggtggaaac 
      aagaacgaa ttcacagcc tacaggata ctgtactat 
      atttttacg aagaatact ttggatatt atgacgatt 
      atgaagagt ctctccaag gaagaaaaa agtgacaag 
      tgtatcgat ttcgcgtta aaattacgt acggcatgg 
      tctttagga aactttcac aggttcttt cagctgtat 
      cttcaggcg cctttaatg gctgggttc ttggtcgat 
      tggtttatt gaacgcgag cgcaaacta tatttaaag 
      tgtattatt aaaaggtag
    • >TC000041 RB
      atggaggag aaacctgct gtctccact gctcctgca 
      gcacccgca ggagcagcg ccccctgaa cctgctcaa 
      acagcccca gtacaatgg gcttacccc atgtacaat 
      tacgacatg agcaattac taccaccaa ttttaccta 
      atgaacagt taccccgcc taccaatcg gcaatttac 
      caccaggtg caacaacaa atgctcgaa aacaaaccg 
      tcaacaaca tcagcggac gctaataaa ttcgtgcct 
      cctctccca ccaggcact ccatcatca ctcccaact 
      ccacacatc tcaaaacca cctcttctg aatactccc 
      aaacaattc accgccaac aacaacaac cccattagg 
      ttcaatctg cctcctggc aaacgccaa aacggcaac 
      ctcctcctc caacagaac aactccctg acaagtggc 
      gccgccaaa aagaaacgc aaacgtaac cgcaacaac 
      caaaaccaa aatttgcag aaccagaac tttttccca 
      cttcctcca ctcccgcca cccgaacaa aacgtaccc 
      aaaccggcg ccgcccccg gaaaccatg ccgccggaa 
      ccgccactg cccccgctt ccggacaca agcaagccg 
      ccgccgcct ccagcggaa aaaccgccc ccgacttcg 
      aataaccca acggatgaa tggccacca agtctcaaa 
      gactacgtg aatcgatgc tatgccaag tgcaagacg 
      aacattgat aagaatcaa gttgagatt attcttaag 
      gggaaaata acgcacgcg tatcagatg ggacagttg 
      catacgaaa gactggagc aacgaaccg ctcccgaat 
      atacacagt gaaagacca acactagta cccaagaca 
      gtacccgga tcattagca cagttccag aataccaca 
      aaaaaggga ctatcacca gcgatggga gcgcgttta 
      ggggcgaga gcgtccacg ctgcgaggt accagcagg 
      tcgtcttct aggtctagg tccaggtcg ccggcgagg 
      aagaagtcc aggtcgagg tccaggagc ccccgcagg 
      catcggtct tcaagcagc agttcgagc gtgagtagc 
      gacgagaac ttcaagcca ttagtgaag cccaacaac 
      aagaacaaa atcaaaagc aaactggcc aatagatta 
      ggcccaaca aagaacaac aaatttaca aacaaacaa 
      ctgaaaaaa cagaaaatg aaagagaaa aaagcacat 
      ttgttatca tcgtttggt tccgaatta gaagagaat 
      tccgaattg ttgcttcaa agagccgca cgtttcaat 
      aacaataaa cagaaagaa acaaatgga ttaataaat 
      aataaaaac gttttcgtg aatgataag tatgaggac 
      aatatcgca gactttgac tggacagga tttcacata 
      gtgggcacg tgtcaagac ttggaaaag tccttcctt 
      cgactgact aaagccccc gaagcatgt gaagtacgt 
      ccagttgaa gttctcaaa ttgtcatta cagaatgtc 
      aagacgaga tggaaacaa aaaccggat tattactac 
      acatgtgat caacttaaa tcaatcaga caagatctc 
      acagtacaa ggagtacgg aacgagttc acggttgaa 
      gtttacgaa actcacgcg cgcatcgct ttagaaaaa 
      ggcgatcac gaagagttc aatcagtgt caaacgcaa 
      ctcaaaatg ttatattcg gaagtcggc ggtggaaac 
      aagaacgaa ttcacagcc tacaggata ctgtactat 
      atttttacg aagaatact ttggatatt atgacgatt 
      atgaagagt ctctccaag gaagaaaaa agtgacaag 
      tgtatcgat ttcgcgtta aaattacgt acggcatgg 
      tctttagga aactttcac aggttcttt cagctgtat 
      cttcaggcg cctttaatg gctgggttc ttggtcgat 
      tggtttatt gaacgcgag cgcaaacta tatttaaag 
      tgtattatt aaaagctat cgtcaaaac atatcagtg 
      aactttatc cagcaagag ttggcgttc ccagcgctg 
      gaggaaact ttaaaattt cttgaaccg ttcagtttg 
      agcttcacg gatcttagc aagagtcat attgattgt 
      aaaaccagt atggcgtct cttccaaat atatga
    mRNA sequence
    • >TC000041 RA
      cgtcacaat tgtcatctg tcatttttg atacagtga 
      tcgtgattt caagatggt cttgtgcgt tatttgtgc 
      attaatttc cacttgatt tgtaatcaa aaataggtg 
      tagaatatt tgtactttt cccataatt tatttacag 
      attaatttc ccttcaaag aaaggatct gccaggtcc 
      cccttaatt tattgattt caggatgga ggagaaacc 
      tgctgtctc cactgctcc tgcagcacc cgcaggagc 
      agcgccccc tgaacctgc tcaaacagc cccagtaca 
      atgggctta ccccatgta caattacga catgagcaa 
      ttactacca ccaatttta cctaatgaa cagttaccc 
      cgcctacca atcggcaat ttaccacca ggtgcaaca 
      acaaatgct cgaaaacaa accgtcaac aacatcagc 
      ggacgctaa taaattcgt gcctcctct cccaccagg 
      cactccatc atcactccc aactccaca catctcaaa 
      accacctct tctgaatac tcccaaaca attcaccgc 
      caacaacaa caaccccat taggttcaa tctgcctcc 
      tggcaaacg ccaaaacgg caacctcct cctccaaca 
      gaacaactc cctgacaag tggcgccgc caaaaagaa 
      acgcaaacg taaccgcaa caaccaaaa ccaaaattt 
      gcagaacca gaacttttt cccacttcc tccactccc 
      gccacccga acaaaacgt acccaaacc ggcgccgcc 
      cccggaaac catgccgcc ggaaccgcc actgccccc 
      gcttccgga cacaagcaa gccgccgcc gcctccagc 
      ggaaaaacc gcccccgac ttcgaataa cccaacgga 
      tgaatggcc accaagtct caaagacta cgtgaatcg 
      atgctatgc caagtgcaa gacgaacat tgataagaa 
      tcaagttga gattattct taaggggaa aataacgca 
      cgcgtatca gatgggaca gttgcatac gaaagactg 
      gagcaacga accgctccc gaatataca cagtgaaag 
      accaacact agtacccaa gacagtacc cggatcatt 
      agcacagtt ccagaatac cacaaaaaa gggactatc 
      accagcgat gggagcgcg tttaggggc gagagcgtc 
      cacgctgcg aggtaccag caggtcgtc ttctaggtc 
      taggtccag gtcgccggc gaggaagaa gtccaggtc 
      gaggtccag gagcccccg caggcatcg gtcttcaag 
      cagcagttc gagcgtgag tagcgacga gaacttcaa 
      gccattagt gaagcccaa caacaagaa caaaatcaa 
      aagcaaact ggccaatag attaggccc aacaaagaa 
      caacaaatt tacaaacaa acaactgaa aaaacagaa 
      aatgaaaga gaaaaaagc acatttgtt atcatcgtt 
      tggttccga attagaaga gaattccga attgttgct 
      tcaaagagc cgcacgttt caataacaa taaacagaa 
      agaaacaaa tggattaat aaataataa aaacgtttt 
      cgtgaatga taagtatga ggacaatat cgcagactt 
      tgactggac aggatttca catagtggg cacgtgtca 
      agacttgga aaagtcctt ccttcgact gactaaagc 
      ccccgaagc atgtgaagt acgtccagt tgaagttct 
      caaattgtc attacagaa tgtcaagac gagatggaa 
      acaaaaacc ggattatta ctacacatg tgatcaact 
      taaatcaat cagacaaga tctcacagt acaaggagt 
      acggaacga gttcacggt tgaagttta cgaaactca 
      cgcgcgcat cgctttaga aaaaggcga tcacgaaga 
      gttcaatca gtgtcaaac gcaactcaa aatgttata 
      ttcggaagt cggcggtgg aaacaagaa cgaattcac 
      agcctacag gatactgta ctatatttt tacgaagaa 
      tactttgga tattatgac gattatgaa gagtctctc 
      caaggaaga aaaaagtga caagtgtat cgatttcgc 
      gttaaaatt acgtacggc atggtcttt aggaaactt 
      tcacaggtt ctttcagct gtatcttca ggcgccttt 
      aatggctgg gttcttggt cgattggtt tattgaacg 
      cgagcgcaa actatattt aaagtgtat tattaaaag 
      gtaggtatg agaggggtc gccacaaac agtgtttcg 
      caaagcgtt tcaaactat tttgagata aattaattg 
      gcgaactcg gaacccttt tctaccctc gatgagtcg 
      cctgagagc aggcatgcc taagttgcc atagaaacg 
      aacttgaat tttcacgta accatagaa acaatgtca 
      cgtaaccag caaggtgac attaatacc agtggcggg 
      ttatttttt tttaatgag aattcagat aagaattag 
      tttatctca aacattgaa actcccttt gcagatgct 
      gtcacgcag caacgtgga aaaattctt aatttaact 
      aactaaagg ggtatttac gctttgtgt gtctcaatc 
      ctcaaacgc ttgagtttg ctttaaatg tgtgtgaaa 
      gcacagtgt tttaaattg tcactttga taaattcga 
      gataattat tttaggtac gtgacactg acattgaca 
      taacactgg acaaaaaaa catttgtta tgatttttt 
      gttcagttt gactcatat ttcatttgt tcatgtcta 
      acagtattt ttttgttta gctatcgtc aaaacatat 
      cagtgaact ttatccagc aagagttgg cgttcccag 
      cgctggagg aaactttaa aatttcttg aaccgttca 
      gtttgagct tcacggatc ttagcaaga gtcatattg 
      attgtaaaa ccagtatgg cgtctcttc caaatatat 
      gaatctgtt tcgttggtt gaaggggtg attgatctt 
      cagtttgtg aaccacaga ctctgtgcc acacttgtc 
      tttaagttt tccaaatat aataacaat aaggtggtc 
      caataagtc ttctaacat ttcttgtct gtggttgct 
      tgattttca atgagaggt gtataagtt ttgaagttt 
      tccttcttt ggtggtgtt tctgtgata cgaggagcc 
      acaagtatt taaaagtct catgtatat aattgtaca 
      taaacaatt tttagat
    • >TC000041 RB
      cgtcacaat tgtcatctg tcatttttg atacagtga 
      tcgtgattt caagatggt cttgtgcgt tatttgtgc 
      attaatttc cacttgatt tgtaatcaa aaataggtg 
      tagaatatt tgtactttt cccataatt tatttacag 
      attaatttc ccttcaaag aaaggatct gccaggtcc 
      cccttaatt tattgattt caggatgga ggagaaacc 
      tgctgtctc cactgctcc tgcagcacc cgcaggagc 
      agcgccccc tgaacctgc tcaaacagc cccagtaca 
      atgggctta ccccatgta caattacga catgagcaa 
      ttactacca ccaatttta cctaatgaa cagttaccc 
      cgcctacca atcggcaat ttaccacca ggtgcaaca 
      acaaatgct cgaaaacaa accgtcaac aacatcagc 
      ggacgctaa taaattcgt gcctcctct cccaccagg 
      cactccatc atcactccc aactccaca catctcaaa 
      accacctct tctgaatac tcccaaaca attcaccgc 
      caacaacaa caaccccat taggttcaa tctgcctcc 
      tggcaaacg ccaaaacgg caacctcct cctccaaca 
      gaacaactc cctgacaag tggcgccgc caaaaagaa 
      acgcaaacg taaccgcaa caaccaaaa ccaaaattt 
      gcagaacca gaacttttt cccacttcc tccactccc 
      gccacccga acaaaacgt acccaaacc ggcgccgcc 
      cccggaaac catgccgcc ggaaccgcc actgccccc 
      gcttccgga cacaagcaa gccgccgcc gcctccagc 
      ggaaaaacc gcccccgac ttcgaataa cccaacgga 
      tgaatggcc accaagtct caaagacta cgtgaatcg 
      atgctatgc caagtgcaa gacgaacat tgataagaa 
      tcaagttga gattattct taaggggaa aataacgca 
      cgcgtatca gatgggaca gttgcatac gaaagactg 
      gagcaacga accgctccc gaatataca cagtgaaag 
      accaacact agtacccaa gacagtacc cggatcatt 
      agcacagtt ccagaatac cacaaaaaa gggactatc 
      accagcgat gggagcgcg tttaggggc gagagcgtc 
      cacgctgcg aggtaccag caggtcgtc ttctaggtc 
      taggtccag gtcgccggc gaggaagaa gtccaggtc 
      gaggtccag gagcccccg caggcatcg gtcttcaag 
      cagcagttc gagcgtgag tagcgacga gaacttcaa 
      gccattagt gaagcccaa caacaagaa caaaatcaa 
      aagcaaact ggccaatag attaggccc aacaaagaa 
      caacaaatt tacaaacaa acaactgaa aaaacagaa 
      aatgaaaga gaaaaaagc acatttgtt atcatcgtt 
      tggttccga attagaaga gaattccga attgttgct 
      tcaaagagc cgcacgttt caataacaa taaacagaa 
      agaaacaaa tggattaat aaataataa aaacgtttt 
      cgtgaatga taagtatga ggacaatat cgcagactt 
      tgactggac aggatttca catagtggg cacgtgtca 
      agacttgga aaagtcctt ccttcgact gactaaagc 
      ccccgaagc atgtgaagt acgtccagt tgaagttct 
      caaattgtc attacagaa tgtcaagac gagatggaa 
      acaaaaacc ggattatta ctacacatg tgatcaact 
      taaatcaat cagacaaga tctcacagt acaaggagt 
      acggaacga gttcacggt tgaagttta cgaaactca 
      cgcgcgcat cgctttaga aaaaggcga tcacgaaga 
      gttcaatca gtgtcaaac gcaactcaa aatgttata 
      ttcggaagt cggcggtgg aaacaagaa cgaattcac 
      agcctacag gatactgta ctatatttt tacgaagaa 
      tactttgga tattatgac gattatgaa gagtctctc 
      caaggaaga aaaaagtga caagtgtat cgatttcgc 
      gttaaaatt acgtacggc atggtcttt aggaaactt 
      tcacaggtt ctttcagct gtatcttca ggcgccttt 
      aatggctgg gttcttggt cgattggtt tattgaacg 
      cgagcgcaa actatattt aaagtgtat tattaaaag 
      ctatcgtca aaacatatc agtgaactt tatccagca 
      agagttggc gttcccagc gctggagga aactttaaa 
      atttcttga accgttcag tttgagctt cacggatct 
      tagcaagag tcatattga ttgtaaaac cagtatggc 
      gtctcttcc aaatatatg aatctgttt cgttggttg 
      aaggggtga ttgatcttc agtttgtga accacagac 
      tctgtgcca cacttgtct ttaagtttt ccaaatata 
      ataacaata aggtggtcc aataagtct tctaacatt 
      tcttgtctg tggttgctt gattttcaa tgagaggtg 
      tataagttt tgaagtttt ccttctttg gtggtgttt 
      ctgtgatac gaggagcca caagtattt aaaagtctc 
      atgtatata attgtacat aaacaattt ttagat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY