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Gene information
General information
TC000137 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10709976..10715675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000137
    iBeetle screen iB_09781

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000137 RA
      MDHQVYYRF AQTNGVPQE SKLANYMNI QNSSGSLHQ 
      SFQSKLSIE PKKPLTSPQ CVGGTSLVS SAPASPNIF 
      NSFTPTLDP SPIRCVSDH PENSSQSAS PNRVSSCSP 
      MSDVNNIAV AAYQENVGG TTYFYPTTS ETCHATDVA 
      VNSNLTSDY NVYPGTPNH ITSLKAKQG ASFFISKDL 
      QMELMNRNA LTLMQANQQ YADLPQVEN YHELCPLER 
      IPSNSLHKT HLGYQASMY KAIHTKTGT PYCLRRLHG 
      FRLQNAKCM VVVDLWKNL NHSNIVQLQ EVFTTKAFG 
      DNSLIFVYD YYPGSETLL SKYFSSDQP DPFAPDTSP 
      RPYSHQKNS ILRHQQNNK LPEFLIWNY IIQLTSALR 
      LIHSSGLAC RSLDPTKII ITSKNRLRL SCLGVMDVV 
      LNENSASVN PLALVQHYQ QEDLTALGK LVLALACKS 
      TVAVQRENI STAVDIISR SYTSDLRNL IMYLLSPQQ 
      RKSITDLMP MIGARFYTQ MDNLHSYIE VLEYELSKE 
      IENGRLFRL MAKLGTINE RPELNMDGA WAETGDRYM 
      LKLFRDYVF HQVTEDGRP WVDMAHIVQ CLNKLDAGV 
      PEKICLMSR DEQSMLVVS YAELKHCLE QSFHEILNA 
      SPVKKDLIE CETEKKV
    CDS sequence
    • >TC000137 RA
      atggatcat caggtttat taccggttc gcacaaacg 
      aatggggtt ccccaggaa tctaaacta gcgaattac 
      atgaacatt caaaattca agtggtagt ttgcaccag 
      tcgttccaa agcaagttg agtatcgaa ccgaaaaaa 
      ccactaacg agtccccaa tgcgttgga gggacaagt 
      ctggtgtcc agtgctcca gcttcaccc aacattttc 
      aactctttc accccaacg ctagaccct tcgcccatt 
      cgatgcgtt tctgaccac ccagaaaat tccagtcaa 
      tcggcttca cccaatcgc gtcagcagc tgttcaccc 
      atgtcagat gtaaataac atagctgtt gcggcatat 
      caggaaaat gtgggcggg acaacatac ttttaccca 
      accacctcg gaaacttgt cacgccact gatgttgct 
      gtcaatagc aacctcacc tcggactac aatgtgtac 
      ccaggcacc cccaaccac atcacttca cttaaggcc 
      aagcagggc gcctcgttt ttcatatca aaggaccta 
      cagatggag ctgatgaat cgcaacgcc ttgacgctc 
      atgcaggcc aatcagcag tacgcagac ctgccgcaa 
      gtggagaac taccacgag ttgtgcccc cttgaacgc 
      atcccgagc aactcgctt cataagacc catttgggg 
      taccaagcg tccatgtac aaagcgatt catactaaa 
      accgggaca ccgtattgc ttgagacgg ctgcatgga 
      tttcgtctc caaaacgcc aagtgtatg gtggtggtc 
      gacttgtgg aaaaatctg aatcattcc aatattgta 
      cagttgcaa gaagtcttc acgactaag gcctttgga 
      gacaattcg ttaattttc gtttatgat tattatccc 
      gggtcggag acactctta agtaagtat ttttcgtcc 
      gatcagccc gatcccttc gcccccgac acctcccct 
      aggccgtac agccaccag aagaacagt atactgcgg 
      caccaacaa aacaacaaa cttccggaa tttctgatt 
      tggaactac attattcag ctcacttcc gccttgcgt 
      ttgatacac tcgtcaggc ctggcctgt aggagcctt 
      gacccgaca aaaataatt atcacttcg aaaaaccga 
      ttaagattg agttgtttg ggtgtgatg gatgtggtt 
      ttgaacgaa aacagtgca tcggtgaat cccctagcc 
      ttagtccag cactaccaa caagaggat ttaacagct 
      ttgggcaag ttagtttta gctttagcg tgcaaatcg 
      acggtggct gtgcaaagg gagaacata tccacagct 
      gtggacatc atcagtcgt tcgtacacg agcgatctt 
      agaaatttg attatgtat ctgttgtcg ccacaacag 
      cggaaaagc atcacggat ttgatgcca atgatcggt 
      gcgaggttt tacacccaa atggacaat ttgcacagt 
      tacatcgaa gtgctggag tacgaactg tcgaaagag 
      atcgaaaat gggcggctt ttcagactt atggccaag 
      ttgggcacg attaacgag cgacctgag ttgaatatg 
      gacggagct tgggccgag accggtgac cgatacatg 
      ctcaaatta tttagggat tacgttttt catcaagtg 
      actgaggat ggccgtcct tgggtcgat atggcacat 
      atagtacaa tgtttaaat aaattggac gccggtgtt 
      cctgaaaag atttgttta atgtcgcgc gacgaacaa 
      agcatgttg gtggtgtcg tacgccgag ttgaaacat 
      tgcctagaa caatcattt catgaaatt ctaaatgct 
      tcgcccgtc aaaaaagac ctcattgag tgtgaaacc 
      gagaagaaa gtgtga
    mRNA sequence
    • >TC000137 RA
      tgcgttgtg ttagcacta tcatatttg ttttttatt 
      ttccgatta ttatcgtta tcttttgca cggtctcgc 
      aggtatttg taccttttt gcgcccttg gtcacatag 
      tgtgttttt ggcaaaatt ggcaacaat taatttttt 
      acatcgccg acaagtcaa atcaacaca aacaaaatg 
      gcgattatt ttcagcaaa ttttcaggt gtcagaagt 
      aaatagaaa taattaatt tcagttttc agaaagggg 
      gttttgtgc gcgttcagg tttctcgac taaaatgtt 
      tccatcgca tggttttta ctagaattg gaatttaat 
      ggatcatca ggtttatta ccggttcgc acaaacgaa 
      tggggttcc ccaggaatc taaactagc gaattacat 
      gaacattca aaattcaag tggtagttt gcaccagtc 
      gttccaaag caagttgag tatcgaacc gaaaaaacc 
      actaacgag tccccaatg cgttggagg gacaagtct 
      ggtgtccag tgctccagc ttcacccaa cattttcaa 
      ctctttcac cccaacgct agacccttc gcccattcg 
      atgcgtttc tgaccaccc agaaaattc cagtcaatc 
      ggcttcacc caatcgcgt cagcagctg ttcacccat 
      gtcagatgt aaataacat agctgttgc ggcatatca 
      ggaaaatgt gggcgggac aacatactt ttacccaac 
      cacctcgga aacttgtca cgccactga tgttgctgt 
      caatagcaa cctcacctc ggactacaa tgtgtaccc 
      aggcacccc caaccacat cacttcact taaggccaa 
      gcagggcgc ctcgttttt catatcaaa ggacctaca 
      gatggagct gatgaatcg caacgcctt gacgctcat 
      gcaggccaa tcagcagta cgcagacct gccgcaagt 
      ggagaacta ccacgagtt gtgccccct tgaacgcat 
      cccgagcaa ctcgcttca taagaccca tttggggta 
      ccaagcgtc catgtacaa agcgattca tactaaaac 
      cgggacacc gtattgctt gagacggct gcatggatt 
      tcgtctcca aaacgccaa gtgtatggt ggtggtcga 
      cttgtggaa aaatctgaa tcattccaa tattgtaca 
      gttgcaaga agtcttcac gactaaggc ctttggaga 
      caattcgtt aattttcgt ttatgatta ttatcccgg 
      gtcggagac actcttaag taagtattt ttcgtccga 
      tcagcccga tcccttcgc ccccgacac ctcccctag 
      gccgtacag ccaccagaa gaacagtat actgcggca 
      ccaacaaaa caacaaact tccggaatt tctgatttg 
      gaactacat tattcagct cacttccgc cttgcgttt 
      gatacactc gtcaggcct ggcctgtag gagccttga 
      cccgacaaa aataattat cacttcgaa aaaccgatt 
      aagattgag ttgtttggg tgtgatgga tgtggtttt 
      gaacgaaaa cagtgcatc ggtgaatcc cctagcctt 
      agtccagca ctaccaaca agaggattt aacagcttt 
      gggcaagtt agttttagc tttagcgtg caaatcgac 
      ggtggctgt gcaaaggga gaacatatc cacagctgt 
      ggacatcat cagtcgttc gtacacgag cgatcttag 
      aaatttgat tatgtatct gttgtcgcc acaacagcg 
      gaaaagcat cacggattt gatgccaat gatcggtgc 
      gaggtttta cacccaaat ggacaattt gcacagtta 
      catcgaagt gctggagta cgaactgtc gaaagagat 
      cgaaaatgg gcggctttt cagacttat ggccaagtt 
      gggcacgat taacgagcg acctgagtt gaatatgga 
      cggagcttg ggccgagac cggtgaccg atacatgct 
      caaattatt tagggatta cgtttttca tcaagtgac 
      tgaggatgg ccgtccttg ggtcgatat ggcacatat 
      agtacaatg tttaaataa attggacgc cggtgttcc 
      tgaaaagat ttgtttaat gtcgcgcga cgaacaaag 
      catgttggt ggtgtcgta cgccgagtt gaaacattg 
      cctagaaca atcatttca tgaaattct aaatgcttc 
      gcccgtcaa aaaagacct cattgagtg tgaaaccga 
      gaagaaagt gtgaaaccg taaaatgtt tgcaataat 
      tttgcttgg tgaattcga aggttttat ttttttata 
      ttattgtga acatttcaa acatcaatc ttttccatc 
      aagaatttt taagaaata ttttttcta tgttgtatt 
      tatttataa gagtaaaat aaaaggaaa attgtgga
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