Status
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Gene information
General information
TC000130 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10774301..10782173
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000130
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09778

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000130 RA
      MDINFVYQK KRSEFGKQC MFSDKGPEL IDNFPSNKK 
      LLRNYILRD PVSRSTHCA PVQAQHFLN TTRAEYTNA 
      AMNHIEGGW PKDVNTADE EQTKRYRRK VEKDEGFHH 
      TVMQLCRNM DNCILQNNA INIYQQYFS DVEPTPLVE 
      RSSARTVNV YQDQSTPSR PVTHISWSP DNQTKLAVS 
      HCNLSFQAV VPNQTTHSY IWEVENPNL PLHVLKPEH 
      PIVCLEYNQ KDPNSLVSG MFNGQVAVW DTRKGHEPV 
      ELSVIENSY RDPVHNVLW INSKSGTEF FGSSTDGQV 
      KWWDTRKLS EPTETLILD ISKDDQSMT NALGASCLE 
      YEPTIPTRF MVGTEQGKV ISCNRKGKT PQEKMIGRF 
      AAHYGPVLA LQRNPAFLK NFLTIGDWT ARIWSEDCR 
      ESSIMWTSF HKALLTDGA WSPTRLSVF FTTRSDGIL 
      DVWDVLQQQ KQACLGVKV CDESLKCLR THEMGRLVA 
      VGNQKGTTY LVEFSENLA MSNKNDKML LTAMFERES 
      KREKILEAR NREIRLKLK ARSALNVPE EGLEEEGKP 
      SAVDLFMDN NVVQAESDF YGVIEAETA EPEPELPPE 
      DEFEGGFDD LEGDTAEES PEVSEPVEE PPKEKKKKT 
      KGKGKKKNK
    CDS sequence
    • >TC000130 RA
      atggatatt aattttgtc tatcaaaag aaacggagc 
      gaattcgga aaacaatgc atgttttcg gacaaaggc 
      cccgaactc atcgacaat ttcccatca aacaagaaa 
      ctattacgc aattatatt ttgagagac ccagtttca 
      cgatcaact cattgtgcc cccgtccaa gcccaacat 
      ttcctcaac acaacacgc gccgaatac accaatgct 
      gcgatgaac cacatcgag gggggctgg cccaaagac 
      gtcaataca gccgatgag gaacaaacg aagagatac 
      aggcgcaaa gtggagaag gacgaagga ttccaccac 
      actgttatg cagctttgc cgcaatatg gataattgc 
      attttgcaa aacaacgcc ataaacatc taccagcag 
      tatttttcg gacgtggag cccacccct ttggtcgag 
      cgcagctcc gctcgaacc gtcaacgtt taccaggac 
      caaagtacc ccatcgcga cccgtcaca cacatatct 
      tggtcccca gacaaccaa actaaactc gcagtttcg 
      cactgcaat ctcagcttt caagccgtt gtcccgaac 
      cagaccaca cattcctac atttgggag gttgaaaac 
      cccaacttg ccccttcat gtcctaaaa ccagaacac 
      ccaatcgtt tgtctggag tacaaccaa aaggacccc 
      aattcgctc gttagtggc atgtttaat gggcaagtt 
      gcagtgtgg gatacacga aaggggcac gaaccggtc 
      gaattgagt gttattgaa aatagttat cgggatcca 
      gtccataac gtgctttgg attaattcc aagtctggg 
      actgagttt tttgggtcg tctactgat gggcaagtc 
      aaatggtgg gatacgagg aaattgtca gaacctacg 
      gaaactctt attctggat ataagtaaa gatgatcaa 
      tcgatgacg aatgcgttg ggggcgtcg tgtctggag 
      tatgaaccg acgattccg acaaggttt atggtgggg 
      actgagcaa gggaaggtt atttcctgt aaccgtaaa 
      ggtaaaaca cctcaggag aaaatgatc ggccgattt 
      gcggcccat tacggccct gtgttggcc cttcagcgc 
      aatccagca tttttgaaa aatttccta acaatcgga 
      gattggaca gctcgcatt tggtccgag gattgtcgc 
      gagagctcc ataatgtgg accagtttc cacaaggcg 
      ttgctcaca gatggcgcc tggagcccc actcgcctt 
      tctgtcttt ttcaccact cgaagtgac ggcattttg 
      gacgtttgg gacgtcctc cagcagcaa aaacaggcc 
      tgtcttggg gtcaaagtg tgcgacgag tcgctcaag 
      tgtctccgg acgcacgaa atgggccgt ttggtggct 
      gtggggaac caaaaaggg accacttat ttggtcgaa 
      tttagcgag aatttagcc atgtcgaat aagaacgat 
      aaaatgttg ttaactgca atgttcgag cgggagagt 
      aaaagggag aagattttg gaagcgagg aatagggaa 
      attcggttg aagctaaag gctcgaagt gcgctaaat 
      gttccggag gaagggctg gaagaggaa gggaaacct 
      tcagctgtt gatttgttt atggacaac aatgtggtg 
      caagctgag agtgatttt tatggcgtt attgaggcc 
      gaaacggcc gaaccggag cccgaattg cccccagaa 
      gatgagttt gaagggggc tttgatgac ttggaagga 
      gacacagcg gaggagagc cccgaagtg agcgaacca 
      gtcgaagaa ccacccaaa gagaaaaag aagaagacc 
      aaggggaaa ggaaagaag aaaaataaa taa
    mRNA sequence
    • >TC000130 RA
      catttcttc agaaaatcg taaagggaa gaacattat 
      tattctccc gtcgacact aaaactctt gataaaggc 
      aaatacgtt atttatttt gccttttag acgcaataa 
      tagtcaaaa ggttcttgt atttgctca caataaact 
      cgtagttat agaaacatc gatttaatt tcatccaaa 
      agtcacagg tcaaagtgt caatttttg cgttttttg 
      ccacaccaa gacttgtaa caaatggat attaatttt 
      gtctatcaa aagaaacgg agcgaattc ggaaaacaa 
      tgcatgttt tcggacaaa ggccccgaa ctcatcgac 
      aatttccca tcaaacaag aaactatta cgcaattat 
      attttgaga gacccagtt tcacgatca actcattgt 
      gcccccgtc caagcccaa catttcctc aacacaaca 
      cgcgccgaa tacaccaat gctgcgatg aaccacatc 
      gaggggggc tggcccaaa gacgtcaat acagccgat 
      gaggaacaa acgaagaga tacaggcgc aaagtggag 
      aaggacgaa ggattccac cacactgtt atgcagctt 
      tgccgcaat atggataat tgcattttg caaaacaac 
      gccataaac atctaccag cagtatttt tcggacgtg 
      gagcccacc cctttggtc gagcgcagc tccgctcga 
      accgtcaac gtttaccag gaccaaagt accccatcg 
      cgacccgtc acacacata tcttggtcc ccagacaac 
      caaactaaa ctcgcagtt tcgcactgc aatctcagc 
      tttcaagcc gttgtcccg aaccagacc acacattcc 
      tacatttgg gaggttgaa aaccccaac ttgcccctt 
      catgtccta aaaccagaa cacccaatc gtttgtctg 
      gagtacaac caaaaggac cccaattcg ctcgttagt 
      ggcatgttt aatgggcaa gttgcagtg tgggataca 
      cgaaagggg cacgaaccg gtcgaattg agtgttatt 
      gaaaatagt tatcgggat ccagtccat aacgtgctt 
      tggattaat tccaagtct gggactgag ttttttggg 
      tcgtctact gatgggcaa gtcaaatgg tgggatacg 
      aggaaattg tcagaacct acggaaact cttattctg 
      gatataagt aaagatgat caatcgatg acgaatgcg 
      ttgggggcg tcgtgtctg gagtatgaa ccgacgatt 
      ccgacaagg tttatggtg gggactgag caagggaag 
      gttatttcc tgtaaccgt aaaggtaaa acacctcag 
      gagaaaatg atcggccga tttgcggcc cattacggc 
      cctgtgttg gcccttcag cgcaatcca gcatttttg 
      aaaaatttc ctaacaatc ggagattgg acagctcgc 
      atttggtcc gaggattgt cgcgagagc tccataatg 
      tggaccagt ttccacaag gcgttgctc acagatggc 
      gcctggagc cccactcgc ctttctgtc tttttcacc 
      actcgaagt gacggcatt ttggacgtt tgggacgtc 
      ctccagcag caaaaacag gcctgtctt ggggtcaaa 
      gtgtgcgac gagtcgctc aagtgtctc cggacgcac 
      gaaatgggc cgtttggtg gctgtgggg aaccaaaaa 
      gggaccact tatttggtc gaatttagc gagaattta 
      gccatgtcg aataagaac gataaaatg ttgttaact 
      gcaatgttc gagcgggag agtaaaagg gagaagatt 
      ttggaagcg aggaatagg gaaattcgg ttgaagcta 
      aaggctcga agtgcgcta aatgttccg gaggaaggg 
      ctggaagag gaagggaaa ccttcagct gttgatttg 
      tttatggac aacaatgtg gtgcaagct gagagtgat 
      ttttatggc gttattgag gccgaaacg gccgaaccg 
      gagcccgaa ttgccccca gaagatgag tttgaaggg 
      ggctttgat gacttggaa ggagacaca gcggaggag 
      agccccgaa gtgagcgaa ccagtcgaa gaaccaccc 
      aaagagaaa aagaagaag accaagggg aaaggaaag 
      aagaaaaat aaataattg aaaaaataa attgtattc 
      aaaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY