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Gene information
General information
TC000118 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10950372..10954469
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000118
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09773

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000118 RA
      MLHNLTATP FHQARTMET MANHLGWNV PKDELIHIP 
      QHWLVYPEP EASMHFLLA LIYIGFFIM ATIGNGLVI 
      WIFSTSKSL RTASNMFVV NLAICDFAM MIKTPIFIY 
      NSFYRGFAL GHLGCQIFA FIGSLSGIG AGMTNACIA 
      YDRYTTITR PFDGKITRT KALVMIIFV WGYTIPWAV 
      MPLLEIWGR FAPEGFLTA CSFDYLTDT FDNHMFVTS 
      IFICSYVIP MSMIIYFYS QIVSKVFSH EKALREQAK 
      KMNVESLRS NQSQQASQS AELRIAKAA IAICSLFVA 
      SWTPYAVLA LIGAFGDQS LLTPGVTMV PACACKFVA 
      CLDPYVYAI SHPKYRLEL QKRLPWLAI KETAASETQ 
      STTTENTTT QSATTTT
    CDS sequence
    • >TC000118 RA
      atgctacac aacttgacc gcaacacct ttccaccaa 
      gctagaaca atggaaacc atggccaac catctcggt 
      tggaacgtc cccaaagac gaactcatt cacataccc 
      caacattgg ctcgtatac cccgaacca gaagcctcc 
      atgcacttc ctcctagct ctaatatac atcggattt 
      ttcataatg gcaacgatc ggaaacgga ctcgtcata 
      tggatattc tcaacatca aaaagtcta cgtacagcc 
      tccaacatg tttgtggta aacctcgcc atctgcgat 
      ttcgcaatg atgatcaaa actccgatt tttatctac 
      aattctttt tatcgtggg tttgctttg ggacatttg 
      ggctgtcaa atttttgcg tttattggt tcgttatcg 
      ggaattggg gccggtatg acaaatgcg tgcatcgca 
      tatgaccga tataccacc ataactagg ccctttgat 
      ggtaaaatc acaagaacc aaagctctg gttatgatt 
      atttttgtt tgggggtac accattccg tgggcagtg 
      atgccacta ctcgaaatt tggggccgc tttgctcca 
      gaggggttc ttaacggcc tgctctttc gactactta 
      accgacacc ttcgacaac cacatgttc gtcacatcc 
      atattcata tgttcctac gtcatacct atgtcaatg 
      ataatttat ttctacagc caaattgtt agtaaagtg 
      ttcagccat gaaaaagcc cttcgagaa caagctaaa 
      aaaatgaac gtcgaatcg ctccgaagc aaccaatca 
      caacaagca agccaatca gccgaacta agaatagcc 
      aaagctgct attgccatt tgttcgcta tttgtagcc 
      tcctggacc ccatatgct gttttagct ctgattggg 
      gccttcgga gaccaatct ttgctcacg cctggagtc 
      acaatggtc ccggcttgt gcatgcaag tttgtggcg 
      tgtttggat ccttatgtg tatgccata agtcatcca 
      aaatacaga cttgagttg cagaaaaga ttgccttgg 
      cttgcgatt aaagaaaca gcggcgagt gaaactcag 
      tcaacgacc acagaaaat accacaact caaagtgct 
      acgactacg acttaa
    mRNA sequence
    • >TC000118 RA
      tattttgca cacatattt ttttccatt ttgaggaga 
      tgggcctaa aggtcccag aaattttac cgccccgac 
      ggcccaact taatccgcc tctagattt ggatgtggc 
      agcttgaat taatttatc tatttattt atttatatc 
      tttgttatt ttacgattt ttacgttaa caattcatt 
      ttaataaat ctattactt tttttgata cattttttt 
      gttttttgt gccccttac aacaataaa tactaaaaa 
      aacagtcat ctcgtttgt cctccactt atccattta 
      atcccaaaa ttccacgtt gcgatttaa taatattaa 
      aaccgtgat taaggtcac ctcgatcct attaaacct 
      tctataaaa tccctcaca acccccaat acctttgtc 
      acttttgtg taaatctct caaaatgct acacaactt 
      gaccgcaac acctttcca ccaagctag aacaatgga 
      aaccatggc caaccatct cggttggaa cgtccccaa 
      agacgaact cattcacat accccaaca ttggctcgt 
      ataccccga accagaagc ctccatgca cttcctcct 
      agctctaat atacatcgg atttttcat aatggcaac 
      gatcggaaa cggactcgt catatggat attctcaac 
      atcaaaaag tctacgtac agcctccaa catgtttgt 
      ggtaaacct cgccatctg cgatttcgc aatgatgat 
      caaaactcc gatttttat ctacaattc tttttatcg 
      tgggtttgc tttgggaca tttgggctg tcaaatttt 
      tgcgtttat tggttcgtt atcgggaat tggggccgg 
      tatgacaaa tgcgtgcat cgcatatga ccgatatac 
      caccataac taggccctt tgatggtaa aatcacaag 
      aaccaaagc tctggttat gattatttt tgtttgggg 
      gtacaccat tccgtgggc agtgatgcc actactcga 
      aatttgggg ccgctttgc tccagaggg gttcttaac 
      ggcctgctc tttcgacta cttaaccga caccttcga 
      caaccacat gttcgtcac atccatatt catatgttc 
      ctacgtcat acctatgtc aatgataat ttatttcta 
      cagccaaat tgttagtaa agtgttcag ccatgaaaa 
      agcccttcg agaacaagc taaaaaaat gaacgtcga 
      atcgctccg aagcaacca atcacaaca agcaagcca 
      atcagccga actaagaat agccaaagc tgctattgc 
      catttgttc gctatttgt agcctcctg gaccccata 
      tgctgtttt agctctgat tggggcctt cggagacca 
      atctttgct cacgcctgg agtcacaat ggtcccggc 
      ttgtgcatg caagtttgt ggcgtgttt ggatcctta 
      tgtgtatgc cataagtca tccaaaata cagacttga 
      gttgcagaa aagattgcc ttggcttgc gattaaaga 
      aacagcggc gagtgaaac tcagtcaac gaccacaga 
      aaataccac aactcaaag tgctacgac tacgactta 
      aaaataatt tattacaaa attaacgaa aacaacgaa 
      ccgcgatga ttatggtca agattttgg aattaattt 
      ttacaataa caaattata gtacttact tacctcttc 
      agttttgtg ttaaatcct tcttgcttt cgaataaat 
      tcctgatta agtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY