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Gene information
General information
TC003649 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10577185..10580318
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003649
    iBeetle screen iB_09771

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003649 RA
      MSSTVQKYF EKYGMAVLE TIGAGVLTY GLFYLMALC 
      FVDCDLELA FYSIFGKSP RKLKGKVVF ITGASSGIG 
      EHTAKALAK HGVRLVLTA RRQSELERV KIECLHLSK 
      NQLSDKDIL VIPFDITDL SAHKRVFQQ ALNHFGTVH 
      VLVNNAGRS QRALFEYID MSVDKQMFD LNVFAVVNL 
      TRIAINHFN EKGSGHVAV VSSLAGVFG APYSATYTA 
      TKHAIHGYF DALRIEKMD KNIAVTLLC PGPTLTNFL 
      QESFTERDG QKYGIPADR KDRRMTGER CGQLCAIAL 
      ANRTQESWM GLFPMMPFV YTAVYFPTI SKMALGLLG 
      PRRLFKMRD SKDIQFDQG KRKL
    CDS sequence
    • >TC003649 RA
      atgtcttca acagtgcaa aagtatttc gaaaaatac 
      ggaatggcc gttctagaa acgataggt gccggtgtt 
      ttaacctac ggtttattc tatttaatg gctctgtgt 
      ttcgtcgat tgtgaccta gagctagct ttctactcg 
      atatttggg aagtcccca cgaaaactg aagggcaaa 
      gtggtcttc atcacaggc gcctccagc ggcattggc 
      gaacacacg gccaaagcc ctagccaaa cacggcgtc 
      cgcctagtg ctcaccgct cgacgccaa agcgaatta 
      gaacgcgtt aaaattgaa tgcctccac ctatccaaa 
      aaccaactc tctgataaa gacatcctg gtgatcccc 
      ttcgacatc accgacttg tccgcccac aagcgggtc 
      ttccagcag gccttgaac cacttcggc accgtgcat 
      gtcctggtg aacaacgcg gggcgctcg cagagggcc 
      ctcttcgag tacattgac atgtccgtc gacaagcaa 
      atgttcgac ttgaacgtg ttcgcagtc gtcaatttg 
      accagaatt gcaatcaac catttcaac gagaagggc 
      tctggtcat gtcgctgtg gtctcgagt cttgcgggg 
      gttttcgga gccccctat tcggccact tacacggcc 
      accaaacac gcaattcat ggatacttt gatgccctc 
      agaattgag aaaatggac aaaaatatc gcagtgacg 
      ctcctgtgt cctgggccc actctcact aatttctta 
      caggaaagc ttcacagaa cgagacggt cagaaatac 
      gggattccg gccgatagg aaagacagg agaatgaca 
      ggggagagg tgtgggcaa ttgtgtgcc attgcgttg 
      gccaatagg acgcaagaa tcctggatg gggctcttt 
      cccatgatg cctttcgtc tacactgct gtatacttc 
      ccaacaatt tcaaaaatg gctttgggt ttattgggg 
      ccccgccga ctattcaaa atgagagac agcaaagat 
      attcagttt gatcagggt aagcgaaaa ctatga
    mRNA sequence
    • >TC003649 RA
      agtgtaaaa gtcaccttg accccgtta tatcgcccc 
      ccggctctc ccactttta cttttattg cctcagtcg 
      cgttccagc cacaacaaa caaacaata atcgtgtga 
      taacaccga taagtgatt tttttaacc aaaaacaaa 
      ttgtggtta tgtcttcaa cagtgcaaa agtatttcg 
      aaaaatacg gaatggccg ttctagaaa cgataggtg 
      ccggtgttt taacctacg gtttattct atttaatgg 
      ctctgtgtt tcgtcgatt gtgacctag agctagctt 
      tctactcga tatttggga agtccccac gaaaactga 
      agggcaaag tggtcttca tcacaggcg cctccagcg 
      gcattggcg aacacacgg ccaaagccc tagccaaac 
      acggcgtcc gcctagtgc tcaccgctc gacgccaaa 
      gcgaattag aacgcgtta aaattgaat gcctccacc 
      tatccaaaa accaactct ctgataaag acatcctgg 
      tgatcccct tcgacatca ccgacttgt ccgcccaca 
      agcgggtct tccagcagg ccttgaacc acttcggca 
      ccgtgcatg tcctggtga acaacgcgg ggcgctcgc 
      agagggccc tcttcgagt acattgaca tgtccgtcg 
      acaagcaaa tgttcgact tgaacgtgt tcgcagtcg 
      tcaatttga ccagaattg caatcaacc atttcaacg 
      agaagggct ctggtcatg tcgctgtgg tctcgagtc 
      ttgcggggg ttttcggag ccccctatt cggccactt 
      acacggcca ccaaacacg caattcatg gatactttg 
      atgccctca gaattgaga aaatggaca aaaatatcg 
      cagtgacgc tcctgtgtc ctgggccca ctctcacta 
      atttcttac aggaaagct tcacagaac gagacggtc 
      agaaatacg ggattccgg ccgatagga aagacagga 
      gaatgacag gggagaggt gtgggcaat tgtgtgcca 
      ttgcgttgg ccaatagga cgcaagaat cctggatgg 
      ggctctttc ccatgatgc ctttcgtct acactgctg 
      tatacttcc caacaattt caaaaatgg ctttgggtt 
      tattggggc cccgccgac tattcaaaa tgagagaca 
      gcaaagata ttcagtttg atcagggta agcgaaaac 
      tatgaagtg gtggtggac tgcacattc ttttgctaa 
      cattttttc ctatttctg cgactaaca aatggcaaa 
      aaattgaat aactggtgg cggtattac ttggttctt 
      gtattattt taaactcct caagctcgt taggtgtaa 
      agaaacgtg atttattta c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY