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Gene information
General information
TC032435
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003615
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9088721..9090795
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032435
    iBeetle screen iB_09770

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032435 RA
      MTTPLTHAQ TGLRDAPPP KRKLTNLPF FLAFIVCLA 
      LVIPFSLVF ALSSNLKRL LNGYDSCGN TCGVENKPL 
      YYRNCSELN MRDKPIAMM KYYDVDPLC IFSLQKVLP 
      DKCVEKCEP PNQLIFNRC YPPEADTNL DSKSRNFKQ 
      MIEIPHVFL SNIATITAM SFTGLILSF GLFACLRCH 
      VAATFWTLT LFQSFVALA AAALFWHKY INSGSTMYL 
      FLALGAMAY FTSVFIYFF VLYNTFTMV RRVYHQALN 
      AIFRAPMIL VQPLLTITV LISVITTMI FLMLLSLSP 
      SGWPEISDE GPLVYEFAT NEATLYYLI YLAFLGFWI 
      YSFGEGFQA MVVSGATVE IYTTKDKTS LRHPVRKSY 
      HTLLHHHLG TVACGSLLA TIFAFMRGL AVYVIRKVY 
      ILTSMKGQP FCKSLKRAV ELLRLNLVE TIALTGFNY 
      LIIYGFSLL IVVITVGVG VWMNLDSKF TFYVRLGIL 
      FGAVSASLV AHFVLKVLH TTTETIFLC YSEERIAGP 
      VTPQNTATK K
    CDS sequence
    • >TC032435 RA
      atgacgact cccttaaca catgcgcaa acaggcctt 
      cgagatgca cctccacca aagcgaaaa cttacaaac 
      ttgccgttt ttcctagca tttattgtc tgtctcgcc 
      cttgtgata ccattttcg cttgttttc gccctaagc 
      agcaatctt aaaagactt ctgaacggg tatgacagc 
      tgcggaaac acttgcgga gtcgaaaac aagccgctt 
      tactatcgc aactgttcg gaactaaat atgagagac 
      aaacccatt gcgatgatg aaatattac gatgttgac 
      ccgttgtgt atttttagc ctacaaaag gttctaccg 
      gacaaatgc gttgaaaag tgcgagccg ccaaaccag 
      ttgattttc aaccggtgt tacccaccc gaagctgac 
      accaacctg gacagcaag tcgagaaat ttcaaacaa 
      atgattgaa atcccccac gtcttcctt agtaacatt 
      gcgaccata actgcgatg agtttcacc ggactgatt 
      ctgtctttc ggtctcttt gcgtgtctt cggtgccac 
      gtcgctgcc accttctgg actttgacc ctattccag 
      tcatttgtg gccctggca gctgcggca cttttctgg 
      cacaagtac ataaacagc ggttctaca atgtactta 
      ttcctggct ttgggggct atggcctat tttacctcc 
      gtgtttatt tacttcttt gtcttgtac aacacgttc 
      acaatggta cggcgcgtt taccaccaa gccttaaat 
      gcgatattt cgagcaccg atgattttg gttcagccc 
      ttattgact atcacggtt ctaatttct gtaatcaca 
      acaatgatt tttctgatg ttgttaagt ttgtcgccc 
      agtggatgg cccgaaata tcagatgag ggtccttta 
      gtctacgaa tttgcaaca aatgaggcc acactttac 
      tacctaatt tacttggct ttcttgggc ttttggatc 
      tattctttt ggggaaggt ttccaggcc atggttgtg 
      tccggggcc acggtagaa atttacacc accaaagac 
      aaaacgtct ttgagacat ccagtcagg aaaagttat 
      cacactttg ctacaccat catttgggt acagtagct 
      tgtggttcc ttgctggcc acaattttt gcatttatg 
      agaggatta gcagtgtat gtgatccgg aaagtttac 
      attttaact tccatgaaa ggacagccc ttctgcaag 
      tccctgaaa cgagccgtc gaattgctg cgtctgaat 
      ctagtcgag acaattgca ctaacaggg tttaattat 
      ttgattatt tacgggttt tccttattg atagttgtg 
      ataacggta ggagtggga gtttggatg aacttggac 
      tccaaattt acgttttac gtccggttg ggtatttta 
      tttggggct gtaagtgcg tcgcttgtg gcacatttc 
      gtactcaaa gtcctacat acaaccacc gaaacgata 
      tttttgtgt tatagtgaa gaacgcata gctggtcct 
      gtgacgcca caaaatacc gcaacaaaa aaataa
    mRNA sequence
    • >TC032435 RA
      gtgtgtgtc aataaattt tttgtaaat aaaaaaccc 
      aaatcttca aaatgacga ctcccttaa cacatgcgc 
      aaacaggcc ttcgagatg cacctccac caaagcgaa 
      aacttacaa acttgccgt ttttcctag catttattg 
      tctgtctcg cccttgtga taccatttt cgcttgttt 
      tcgccctaa gcagcaatc ttaaaagac ttctgaacg 
      ggtatgaca gctgcggaa acacttgcg gagtcgaaa 
      acaagccgc tttactatc gcaactgtt cggaactaa 
      atatgagag acaaaccca ttgcgatga tgaaatatt 
      acgatgttg acccgttgt gtattttta gcctacaaa 
      aggttctac cggacaaat gcgttgaaa agtgcgagc 
      cgccaaacc agttgattt tcaaccggt gttacccac 
      ccgaagctg acaccaacc tggacagca agtcgagaa 
      atttcaaac aaatgattg aaatccccc acgtcttcc 
      ttagtaaca ttgcgacca taactgcga tgagtttca 
      ccggactga ttctgtctt tcggtctct ttgcgtgtc 
      ttcggtgcc acgtcgctg ccaccttct ggactttga 
      ccctattcc agtcatttg tggccctgg cagctgcgg 
      cacttttct ggcacaagt acataaaca gcggttcta 
      caatgtact tattcctgg ctttggggg ctatggcct 
      attttacct ccgtgttta tttacttct ttgtcttgt 
      acaacacgt tcacaatgg tacggcgcg tttaccacc 
      aagccttaa atgcgatat ttcgagcac cgatgattt 
      tggttcagc ccttattga ctatcacgg ttctaattt 
      ctgtaatca caacaatga tttttctga tgttgttaa 
      gtttgtcgc ccagtggat ggcccgaaa tatcagatg 
      agggtcctt tagtctacg aatttgcaa caaatgagg 
      ccacacttt actacctaa tttacttgg ctttcttgg 
      gcttttgga tctattctt ttggggaag gtttccagg 
      ccatggttg tgtccgggg ccacggtag aaatttaca 
      ccaccaaag acaaaacgt ctttgagac atccagtca 
      ggaaaagtt atcacactt tgctacacc atcatttgg 
      gtacagtag cttgtggtt ccttgctgg ccacaattt 
      ttgcattta tgagaggat tagcagtgt atgtgatcc 
      ggaaagttt acattttaa cttccatga aaggacagc 
      ccttctgca agtccctga aacgagccg tcgaattgc 
      tgcgtctga atctagtcg agacaattg cactaacag 
      ggtttaatt atttgatta tttacgggt tttccttat 
      tgatagttg tgataacgg taggagtgg gagtttgga 
      tgaacttgg actccaaat ttacgtttt acgtccggt 
      tgggtattt tatttgggg ctgtaagtg cgtcgcttg 
      tggcacatt tcgtactca aagtcctac atacaacca 
      ccgaaacga tatttttgt gttatagtg aagaacgca 
      tagctggtc ctgtgacgc cacaaaata ccgcaacaa 
      aaaaataaa tctggcctt gttttaatt ttatgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY