Status
Please wait ...
Gene information
General information
TC030612 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8834143..8836118
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030612
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09768

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber stage sequence irregular - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 2
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030612 RA
      MTNSHSPCQ IGDRIESGG YIGTVKYVG PIEGKPSIW 
      LGIDWDNPQ RGKHDGRVN GVQYFTTRN PTSGSFIRP 
      EKVNCGKSA LASIESRYG QIEDEFTAK INKQKQLLI 
      QQNMNAPFL EMVGFDKVF HKQSDFTAL RIVNLRDQG 
      INSAGPPLR LGETCPNIE ELDISKNLL VSWESVFEI 
      CRQLPRLFW LNVSENLLD LPTISESFP NVTTLICGC 
      MDLDWGHIC QLGRIFPSV EEFRAPNNK IRGLSTPEG 
      FFTKLKLLD LEGNNIEFW TEVCKLGDL PHLEQLILE 
      DIGLQLIEF EGDSPKVQV FRAMKKLCV VKNLIREWR 
      SVAELNRLE SLENLRFSK NPITESEEP DTIHQIIIA 
      KIANLKILN GVEIEATER RGAEYDYMK KYGLEWLEA 
      KGTNKEADF LKTHNRYLE LIQKYGELE ESELVSKPK 
      IIKNTLVQL SIIFEDFTI EKKVPLTMT VQKLIVLTQ 
      KLFSLSGRP HLIYASKSD PEIEILLED EMKEIGVYS 
      MQNGDKIIV KDKV
    CDS sequence
    • >TC030612 RA
      atgacaaat tcgcactct ccttgccaa attggggac 
      cgcatcgaa agcggcggt tacatcgga acggtaaaa 
      tacgtgggc cccatcgag ggcaagccc tcaatttgg 
      ctcggaatc gactgggac aacccccag cggggcaaa 
      cacgacggc cgcgtcaac ggagtgcaa tatttcacc 
      accagaaac ccgacttca ggttccttc ataaggcct 
      gagaaggtc aattgcggg aaatcagcc ctagcgtcg 
      attgagtcg cgctacggc cagatcgag gatgaattc 
      accgccaaa atcaacaaa cagaagcag ctccttatc 
      caacaaaac atgaacgcg ccttttttg gaaatggtt 
      ggctttgac aaggttttc cacaaacaa agcgatttt 
      acggcgttg cgaatcgtc aatttgcgt gatcaaggg 
      attaattcg gcgggtccg ccgctacga ctcggagaa 
      acgtgtccg aatattgaa gagttggac atttcgaaa 
      aatttgcta gtgtcttgg gagagcgtt tttgagatt 
      tgccgccag ttgccgagg cttttttgg ttgaatgtt 
      agtgagaat ttgctggac ttgccgacg attagtgag 
      agttttcca aatgtgaca actctgatt tgtgggtgt 
      atggacttg gactggggg cacatttgc caacttgga 
      cgcattttc ccgtcagtt gaagagttc agagctccg 
      aataacaaa attcggggg cttagcaca cccgagggg 
      tttttcacc aaactaaaa ttgctcgat cttgaaggg 
      aataacatt gagttttgg acagaggtc tgcaaattg 
      ggggattta ccgcacttg gagcaactt attctggag 
      gatattggg ctccagttg attgagttt gagggtgat 
      tcccccaaa gtacaagtt ttcagagcg atgaaaaaa 
      ttatgtgtt gtgaaaaac ttgatccgt gagtggcgt 
      tctgtggct gagttaaat cggcttgaa agtttagaa 
      aatctgcga ttttccaaa aaccccatc acagaatcg 
      gaagaacct gacactatc catcaaata ataatcgca 
      aaaattgcc aacttgaag attttaaat ggggttgag 
      attgaagcc actgaaagg cggggggct gagtacgac 
      tacatgaaa aaatatggt ctggaatgg ttggaagcc 
      aagggtacc aacaaagag gctgatttt ctcaaaacg 
      cacaacaga tatttggaa ctgatacaa aaatatggc 
      gagctggaa gagagtgaa cttgtctca aaaccgaaa 
      attattaaa aatacgttg gtccaatta agtataatt 
      tttgaggac tttacgata gaaaagaaa gtgcctctc 
      acaatgact gtgcaaaaa ctcatagtc ctcacacaa 
      aaactgttc agtttgagt ggaaggccc cacttgatt 
      tatgccagc aagagcgat ccagaaatc gagattctt 
      ctagaggat gaaatgaaa gaaatcgga gtttactca 
      atgcaaaat ggtgataaa atcatcgtg aaagacaaa 
      gtgtga
    mRNA sequence
    • >TC030612 RA
      atcacaaat gtcaaaaaa attgtgatt catattact 
      tttaaatca ataatgtgt tgtatgtat tgaaataat 
      tgtaacaat ttggccgta cggtataaa tttagcttg 
      attctaacc cacctgact tgaaaaaaa catcacagc 
      tatgacaaa ttcgcactc tccttgcca aattgggga 
      ccgcatcga aagcggcgg ttacatcgg aacggtaaa 
      atacgtggg ccccatcga gggcaagcc ctcaatttg 
      gctcggaat cgactggga caaccccca gcggggcaa 
      acacgacgg ccgcgtcaa cggagtgca atatttcac 
      caccagaaa cccgacttc aggttcctt cataaggcc 
      tgagaaggt caattgcgg gaaatcagc cctagcgtc 
      gattgagtc gcgctacgg ccagatcga ggatgaatt 
      caccgccaa aatcaacaa acagaagca gctccttat 
      ccaacaaaa catgaacgc gcctttttt ggaaatggt 
      tggctttga caaggtttt ccacaaaca aagcgattt 
      tacggcgtt gcgaatcgt caatttgcg tgatcaagg 
      gattaattc ggcgggtcc gccgctacg actcggaga 
      aacgtgtcc gaatattga agagttgga catttcgaa 
      aaatttgct agtgtcttg ggagagcgt ttttgagat 
      ttgccgcca gttgccgag gcttttttg gttgaatgt 
      tagtgagaa tttgctgga cttgccgac gattagtga 
      gagttttcc aaatgtgac aactctgat ttgtgggtg 
      tatggactt ggactgggg gcacatttg ccaacttgg 
      acgcatttt cccgtcagt tgaagagtt cagagctcc 
      gaataacaa aattcgggg gcttagcac acccgaggg 
      gtttttcac caaactaaa attgctcga tcttgaagg 
      gaataacat tgagttttg gacagaggt ctgcaaatt 
      gggggattt accgcactt ggagcaact tattctgga 
      ggatattgg gctccagtt gattgagtt tgagggtga 
      ttcccccaa agtacaagt tttcagagc gatgaaaaa 
      attatgtgt tgtgaaaaa cttgatccg tgagtggcg 
      ttctgtggc tgagttaaa tcggcttga aagtttaga 
      aaatctgcg attttccaa aaaccccat cacagaatc 
      ggaagaacc tgacactat ccatcaaat aataatcgc 
      aaaaattgc caacttgaa gattttaaa tggggttga 
      gattgaagc cactgaaag gcggggggc tgagtacga 
      ctacatgaa aaaatatgg tctggaatg gttggaagc 
      caagggtac caacaaaga ggctgattt tctcaaaac 
      gcacaacag atatttgga actgataca aaaatatgg 
      cgagctgga agagagtga acttgtctc aaaaccgaa 
      aattattaa aaatacgtt ggtccaatt aagtataat 
      ttttgagga ctttacgat agaaaagaa agtgcctct 
      cacaatgac tgtgcaaaa actcatagt cctcacaca 
      aaaactgtt cagtttgag tggaaggcc ccacttgat 
      ttatgccag caagagcga tccagaaat cgagattct 
      tctagagga tgaaatgaa agaaatcgg agtttactc 
      aatgcaaaa tggtgataa aatcatcgt gaaagacaa 
      agtgtgatt gattttgtg ttgtacaaa accattaat 
      ttattcaat aaagttttt aataaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY