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Gene information
General information
TC011042 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6190683..6193294
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011042
    iBeetle screen iB_09767

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011042 RA
      MVVIQRVVK ATTRKGKKV LLKKEPQVV EGPKKALFF 
      QGRRSSEKL RRLAKDLYD LKKPEAQML NKKNDITIF 
      ENATPVEAF CKKYESSLF VMSSHSKKR PDNLVIGRM 
      FNYSLLDMV ELHVEAAEF MKDFACSKI ALGTKPCLV 
      FNGPAWEET EVLKHLKSL FIDMFHREE VNSVRLQGL 
      EHTISFTAT PEGKILIRS YKVMMKKSG CRVPRVELE 
      EMGPRIDLT LRRSKLPSE DLMKEALRK PKELKVSKK 
      KNISVDGLG TTHGRIHVG KQEINKIQT RKMKGLKKT 
      TDEKKLKRA AKRTTDNNN VNNKVKKQK LG
    CDS sequence
    • >TC011042 RA
      atggttgta atccagcgt gtggttaag gcgaccaca 
      cgtaaaggc aaaaaggtg ctcctgaag aaagagccg 
      caagtggtt gagggcccc aagaaggcg ctctttttc 
      cagggccgg cgctcgtcg gagaaactg cgccgtttg 
      gcgaaagat ctgtacgat ttgaagaag ccggaggcg 
      caaatgctc aacaagaaa aatgatata acgattttt 
      gaaaatgcc actcctgtg gaggcgttc tgtaagaaa 
      tatgagtcg tctctgttc gttatgagt tcacatagt 
      aaaaaaagg ccggacaat ctagtcatt gggaggatg 
      tttaattat agtctgctg gatatggtg gagctgcat 
      gtggaggct gctgagttc atgaaagac tttgcctgc 
      tcgaaaata gcgctcggg acgaagccc tgtctcgtg 
      ttcaacggg ccggcgtgg gaagagact gaagtgttg 
      aaacatctc aagagtttg ttcattgat atgtttcac 
      agggaggag gttaattca gtcaggttg caagggttg 
      gagcacact attagcttt actgctacg ccagaaggg 
      aagattttg attaggtcg tacaaagtt atgatgaag 
      aagtcaggg tgtcgggtg ccgcgggtg gaacttgaa 
      gaaatgggt cccagaata gacctcacc cttcgccga 
      agcaaactc cccagcgaa gacctaatg aaagaagcc 
      ttgcgcaaa cctaaagaa ctcaaagtg agcaaaaag 
      aagaacatt agtgttgac ggcttgggc acaacccac 
      ggacgcatc cacgtcggt aaacaagaa atcaacaaa 
      atccaaacc cgcaaaatg aagggctta aaaaaaacc 
      accgacgaa aagaaactc aaacgagca gcaaagaga 
      acaactgac aataacaat gtaaataat aaagtcaaa 
      aagcaaaag cttggataa
    mRNA sequence
    • >TC011042 RA
      tggagcagg ttagttttc attccaaca cgtgtggac 
      aagtttaca ctgaaaaaa ctgtgaaaa taagcgcaa 
      aaactagtg aaaatggtt gtaatccag cgtgtggtt 
      aaggcgacc acacgtaaa ggcaaaaag gtgctcctg 
      aagaaagag ccgcaagtg gttgagggc cccaagaag 
      gcgctcttt ttccagggc cggcgctcg tcggagaaa 
      ctgcgccgt ttggcgaaa gatctgtac gatttgaag 
      aagccggag gcgcaaatg ctcaacaag aaaaatgat 
      ataacgatt tttgaaaat gccactcct gtggaggcg 
      ttctgtaag aaatatgag tcgtctctg ttcgttatg 
      agttcacat agtaaaaaa aggccggac aatctagtc 
      attgggagg atgtttaat tatagtctg ctggatatg 
      gtggagctg catgtggag gctgctgag ttcatgaaa 
      gactttgcc tgctcgaaa atagcgctc gggacgaag 
      ccctgtctc gtgttcaac gggccggcg tgggaagag 
      actgaagtg ttgaaacat ctcaagagt ttgttcatt 
      gatatgttt cacagggag gaggttaat tcagtcagg 
      ttgcaaggg ttggagcac actattagc tttactgct 
      acgccagaa gggaagatt ttgattagg tcgtacaaa 
      gttatgatg aagaagtca gggtgtcgg gtgccgcgg 
      gtggaactt gaagaaatg ggtcccaga atagacctc 
      acccttcgc cgaagcaaa ctccccagc gaagaccta 
      atgaaagaa gccttgcgc aaacctaaa gaactcaaa 
      gtgagcaaa aagaagaac attagtgtt gacggcttg 
      ggcacaacc cacggacgc atccacgtc ggtaaacaa 
      gaaatcaac aaaatccaa acccgcaaa atgaagggc 
      ttaaaaaaa accaccgac gaaaagaaa ctcaaacga 
      gcagcaaag agaacaact gacaataac aatgtaaat 
      aataaagtc aaaaagcaa aagcttgga taacgcgtc 
      ggtgcaatc ttgttgatg attattgtg tataaatgg 
      ttaaattgt ctagtttta agcctagtt ttacgtgta 
      ttttttatt taatgacta tttcaataa actttttta 
      taaaat
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