Status
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Gene information
General information
TC003523 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6233282..6237939
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003523
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09762

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3
    vitellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003523 RA
      MNLLSAFCL IATAASLGW ASEEIKSDE GVLVLTKSN 
      FKQAITDNE FILVEFYAP WCGHCKALA PEYVKAAKA 
      LADQDSKIK LGKVDATEE TELAEEHQV RGYPTLKFF 
      RNGSPIDYN GGRQADDIV AWLLKKTGP PAKEIKTVE 
      EAKEFIDAS NVAVIGFFK DQTTDKAKA FLAAAATID 
      DYPFGITSE DSVYKEYEA ECGSIVLFK KFDEGKVLF 
      EGEATEKNI KKFVAGNSL PLIVEFNHE TAQKIFGGD 
      IKSHLLLFL NKGEDHFEK VSEAARAVA KPFKEQVLF 
      VTIDAGEED HQRILEFFG MKKEEVPAA RLIKLEEDM 
      AKYKPETDE LSSESIKKF VEDFLAGKL KQHLLSQDL 
      PEDWDKEAV KVLVATNFD SVVFDADKD VLVEFYAPW 
      CGHCKQLAP IYDKVGEHF KDDKSVVVA KIDATANEL 
      EHTKITSFP TLKFYPKGG NNVIEYNGP RTFEGLVKF 
      IESGGVDGA GVDEPVEEE TEDDDSPRK DEL
    CDS sequence
    • >TC003523 RA
      atgaatctt ctttcggct ttttgttta attgcaacg 
      gctgcatct ctgggctgg gccagtgaa gaaattaag 
      agcgacgaa ggcgtccta gtgctcacc aaatccaac 
      ttcaaacag gccatcacc gacaacgaa ttcatcctt 
      gtagagttt tatgcccca tggtgtgga cattgcaag 
      gcgttagcc cccgaatat gtaaaggca gcgaaagcg 
      ctcgccgac caagactct aaaattaaa ctcggcaaa 
      gttgacgcc actgaagaa accgaactt gctgaagaa 
      caccaagtt agaggttac ccaaccttg aagttcttc 
      agaaacgga agtcccatt gactacaac ggtggcaga 
      caagccgat gatattgtc gcctggctt ttgaaaaaa 
      actggacca ccagcgaaa gaaatcaaa actgtcgaa 
      gaagccaag gagtttatt gatgccagc aatgttgct 
      gttattgga tttttcaaa gatcagacg actgataag 
      gcaaaggcc ttcctggcg gcagccgcc accatcgac 
      gattatccc ttcggaatc acctctgaa gacagcgtc 
      tacaaggag tacgaagcc gaatgcgga tccattgtt 
      ttgttcaaa aagttcgat gaaggaaaa gttcttttc 
      gaaggcgaa gccactgag aagaacatc aagaaattc 
      gtcgctggt aactcattg cccctcatc gtcgaattc 
      aaccatgaa acagcccag aagatcttc ggtggcgac 
      atcaagagc caccttttg ttgttcttg aacaagggc 
      gaagaccac ttcgagaag gtgtccgag gctgcaaga 
      gccgtcgcc aagcccttc aaggaacaa gttttgttc 
      gtcacaatc gacgccggc gaagaagac caccaaaga 
      atcttggaa ttcttcgga atgaagaaa gaggaagtc 
      ccggcggcg cgtctcatc aagcttgag gaagacatg 
      gccaagtac aagccagaa accgatgaa ttatcctct 
      gaaagtatc aagaaattc gtcgaagat ttcctcgcc 
      ggtaaactc aaacagcat ttgctcagt caagatctt 
      ccagaagat tgggataag gaggcggtt aaggttcta 
      gtcgccacc aacttcgac agtgtggta tttgacgcc 
      gacaaggac gttttggtc gagttctac gccccgtgg 
      tgcggccac tgcaagcag ttggccccc atttacgac 
      aaggtgggc gagcacttc aaagatgac aagagcgtg 
      gtcgtggcg aagatcgat gccaccgct aacgaactc 
      gaacatacc aaaattacg agcttccct acattgaaa 
      ttctatccc aaaggtggg aacaacgtt attgaatat 
      aacggtcca agaacattc gaaggttta gttaaattt 
      atcgagtcc ggtggtgtt gacggtgcc ggtgttgac 
      gagcccgtc gaagaggaa actgaagat gacgactca 
      cccagaaaa gatgagttg taa
    mRNA sequence
    • >TC003523 RA
      cgtcgattg ttaataggg gaagtgagt tgtattaaa 
      aaattaaga tgaatcttc tttcggctt tttgtttaa 
      ttgcaacgg ctgcatctc tgggctggg ccagtgaag 
      aaattaaga gcgacgaag gcgtcctag tgctcacca 
      aatccaact tcaaacagg ccatcaccg acaacgaat 
      tcatccttg tagagtttt atgccccat ggtgtggac 
      attgcaagg cgttagccc ccgaatatg taaaggcag 
      cgaaagcgc tcgccgacc aagactcta aaattaaac 
      tcggcaaag ttgacgcca ctgaagaaa ccgaacttg 
      ctgaagaac accaagtta gaggttacc caaccttga 
      agttcttca gaaacggaa gtcccattg actacaacg 
      gtggcagac aagccgatg atattgtcg cctggcttt 
      tgaaaaaaa ctggaccac cagcgaaag aaatcaaaa 
      ctgtcgaag aagccaagg agtttattg atgccagca 
      atgttgctg ttattggat ttttcaaag atcagacga 
      ctgataagg caaaggcct tcctggcgg cagccgcca 
      ccatcgacg attatccct tcggaatca cctctgaag 
      acagcgtct acaaggagt acgaagccg aatgcggat 
      ccattgttt tgttcaaaa agttcgatg aaggaaaag 
      ttcttttcg aaggcgaag ccactgaga agaacatca 
      agaaattcg tcgctggta actcattgc ccctcatcg 
      tcgaattca accatgaaa cagcccaga agatcttcg 
      gtggcgaca tcaagagcc accttttgt tgttcttga 
      acaagggcg aagaccact tcgagaagg tgtccgagg 
      ctgcaagag ccgtcgcca agcccttca aggaacaag 
      ttttgttcg tcacaatcg acgccggcg aagaagacc 
      accaaagaa tcttggaat tcttcggaa tgaagaaag 
      aggaagtcc cggcggcgc gtctcatca agcttgagg 
      aagacatgg ccaagtaca agccagaaa ccgatgaat 
      tatcctctg aaagtatca agaaattcg tcgaagatt 
      tcctcgccg gtaaactca aacagcatt tgctcagtc 
      aagatcttc cagaagatt gggataagg aggcggtta 
      aggttctag tcgccacca acttcgaca gtgtggtat 
      ttgacgccg acaaggacg ttttggtcg agttctacg 
      ccccgtggt gcggccact gcaagcagt tggccccca 
      tttacgaca aggtgggcg agcacttca aagatgaca 
      agagcgtgg tcgtggcga agatcgatg ccaccgcta 
      acgaactcg aacatacca aaattacga gcttcccta 
      cattgaaat tctatccca aaggtggga acaacgtta 
      ttgaatata acggtccaa gaacattcg aaggtttag 
      ttaaattta tcgagtccg gtggtgttg acggtgccg 
      gtgttgacg agcccgtcg aagaggaaa ctgaagatg 
      acgactcac ccagaaaag atgagttgt aaattatta 
      ggtacttag ttttgttac taacattct aaattactc 
      attttgtat atcaaaaaa ttcgcggcg attttggct 
      ctcggtttt gtaggtacc tatttgaac ttcccaaat 
      gttttattt gcgcctcca tttacggag gatttgtga 
      gactggttg tgcaattga tatctgcct gacattaac 
      gagcaagtt ctttattaa cattcctgt cttctctat 
      attctctta aattgttta taattgcta tttcgtgta 
      tttattttt tattcattt catagatga tatttttct 
      actgattat tgtgtcgta gcgatttag gttgagcac 
      aaatggcag attgtgttg tgtgtttgt gtgactgta 
      tatttttta acattaaag aattgtaaa ctaagatat 
      tttttgtga attactgta agacgaagc tgaaacaaa 
      taaatgaaa ttgtatgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY