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Gene information
General information
TC002926 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6189321..6192428
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002926
    iBeetle screen iB_09761

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002926 RA
      MDFGDDLGV LSDGSNDSF FDEPKPASK RASLSKTPE 
      KKSITDLFN ITDKTPESN QVPKRPDDW LGLDSRDTK 
      SPSRSQRLA KKISFDDDD DILGNLGLT KKPQSKPEE 
      NKTPTKKMD LLESILGPK SEDVKTATF EDILKESKA 
      KTVTPTTTP KLTPSNPPS ESVLLSEGP REGRRRRRS 
      SAGLLDPLG LFSNEIKTD DSASKEKII TSSTETPVS 
      KPTPSKSAP NLSDQGLPD WLGGGSAIK QSKSENILP 
      AKQEPEKSI EPAKESDQS SINTILTQQ KLAQSHMEY 
      QNTAIALQQ QESQLLMAL QLKKYEDNL SDMQMKQQE 
      VLMKQEQQF NLLLERQFA KQQIMENNM RLQQERINN 
      HIQMLLAQP PIGKHSGQE EIDDLKKTA SEENVKLYE 
      NIINSLKQR QHEEIFLLE ESYKKQINL LEQSLESVE 
      RRLKTDVEK MTEVFEEKL KTVLQQHDG EIAKYKQRI 
      EEADAHHSE EIKRIRENN SRVIEEIKY EYTTLLENV 
      KEAKKSESS LFQESNTYL QKLDSNIEI LYANSKSLG 
      DLKDHIQKD YGILSRARE ESLKAKEQE IILMRSTLE 
      KCREEAEKE RAELLALVR TLETKISEQ NQNAREERW 
      AFQQAASTL AARSAAMDR EAEFNRAAL EREREQLKT 
      LRESILAEQ EKTMLQLSE EKLSLAAEK SRFETSNKL 
      MVNYDSQKA KAEIDAAVQ IAKEAAEMA DKERESLHK 
      EKCEMEKLK RCLQDQERK LSLRETEVE NLMKEAQKK 
      VNEGEKAIF ESKTIENKY NERLRDLQN QLNMLSNRE 
      KKLAEEKIA LAKERLSLN TLMRQSKKC SLCSADPQK 
      LEESHLNIN SEVLTSYFS RYDNIDNDI LRLRLEATE 
      ENLLNINHD VPN
    CDS sequence
    • >TC002926 RA
      atggatttt ggggacgat ttaggggtg ctatccgat 
      ggcagcaac gacagtttc tttgacgag cccaaaccg 
      gcatccaaa agggcaagt ttgagcaaa acaccggaa 
      aaaaagtcc atcacagat ctattcaat ataaccgac 
      aaaacaccc gaaagcaat caagtgcca aaacgaccg 
      gacgactgg ttgggatta gactccaga gacaccaaa 
      tctccttct cggagccaa agattggcc aaaaaaatc 
      agcttcgat gacgacgac gatattttg ggcaatttg 
      ggtctgacc aagaaacct cagagtaaa cccgaagaa 
      aataaaaca ccgacgaag aaaatggat ctgcttgaa 
      agtattttg ggaccaaaa agtgaagat gttaaaaca 
      gcgactttt gaggatatt ttgaaagag agtaaagca 
      aaaactgtg actccgacg accactcca aagctgaca 
      ccttcaaat cctccatca gagtctgtc ttgttgagc 
      gaagggcct cgggaaggt cggcgaagg cgtaggtct 
      tcggctgga ttattggac cctctaggc ttatttagc 
      aacgaaata aaaactgat gattctgct tctaaagaa 
      aaaataata accagctct actgaaact cctgtttcc 
      aaacctaca ccgtcgaaa agtgctcca aacctgtcc 
      gatcaaggt cttccggat tggcttggt ggcggatcc 
      gcaatcaag caaagcaaa tcagaaaat atattacca 
      gcaaaacaa gaacccgaa aaatcgata gaaccagca 
      aaagaaagc gaccaatct tccattaac actatttta 
      acacagcaa aagttggcc cagtcccac atggagtat 
      caaaatacg gctattgcc ctccaacag caagaatcg 
      caactactg atggccctc cagttgaaa aaatacgaa 
      gacaattta tccgacatg caaatgaag caacaggaa 
      gttttaatg aaacaagag caacagttt aatttgctt 
      ctagaaagg cagtttgct aaacaacaa atcatggaa 
      aataatatg cgccttcaa caagagaga attaataac 
      catattcaa atgctatta gcgcaacca ccaattggc 
      aaacattcg ggtcaagag gaaattgac gatttaaaa 
      aagacagcg agtgaggaa aacgtgaag ctttacgaa 
      aacataatc aactcgttg aaacagaga cagcacgag 
      gaaattttc ctactggag gagtcctac aagaaacaa 
      atcaatttg ttagaacaa agtctggaa tcagtcgaa 
      aggcggttg aaaactgac gttgaaaaa atgaccgaa 
      gtgtttgag gaaaaatta aaaacagta ctacagcaa 
      cacgatggt gaaattgca aaatacaag caaagaatt 
      gaagaggct gacgcgcac cattccgaa gaaataaaa 
      cggattaga gaaaataat tcccgagtt attgaggaa 
      attaaatac gaatacacc accctcttg gagaatgtc 
      aaagaggcc aaaaaatcc gaatcgtcg ttattccag 
      gaatccaat acttacctt cagaaactg gacagcaat 
      attgaaatt ctgtacgct aacagcaaa agtttgggg 
      gacctcaaa gaccacatc cagaaggat tatggtata 
      ttatcgcgg gcgcgggaa gagtccttg aaagctaag 
      gagcaggag attattctg atgaggagt acgttggaa 
      aaatgtcgc gaagaagcc gaaaaggag agagcggag 
      cttttggcg ttggtgcgc accttggag actaaaata 
      tcggagcaa aaccaaaat gcccgtgaa gaaaggtgg 
      gcttttcag caagcggct agcacttta gctgcgcgc 
      tcagctgcc atggacaga gaagctgag tttaataga 
      gcagctctc gaacgtgaa agagaacag ttaaagact 
      ttgagggaa tcaattttg gccgaacaa gagaaaacc 
      atgttgcaa ctctccgag gaaaaatta tcgctcgca 
      gctgaaaaa tcccggttc gaaacgtcc aataaatta 
      atggtcaat tacgactcg caaaaggcc aaagccgaa 
      atcgacgct gctgtgcag attgcgaaa gaagcggcc 
      gaaatggct gataaagag cgggaaagt cttcataaa 
      gaaaaatgc gagatggaa aaactaaag cgatgtttg 
      caagaccag gaacgtaag ctgagtttg agggaaaca 
      gaagtagag aatttaatg aaagaagct caaaagaag 
      gtaaacgaa ggtgaaaaa gccattttt gagtccaaa 
      actatcgaa aataagtac aacgaaaga ctgagggat 
      ttacaaaat cagctaaac atgttatcg aatagagaa 
      aagaagtta gctgaggag aaaatagcg ctcgctaag 
      gaacgtttg tcattgaac actctcatg cgtcagagt 
      aagaagtgc agtctttgc agtgctgat ccacaaaaa 
      cttgaagaa agtcatctt aacataaac tcggaggtt 
      ctgacgtct tatttttcg agatatgat aatattgac 
      aatgacatc cttcgtctt cgtttggaa gctactgag 
      gaaaatcta ttgaacata aaccacgat gtgccaaac 
      tag
    mRNA sequence
    • >TC002926 RA
      ttacaagat ttataaaaa aagaaattt gttcttttg 
      cgggctttg ttgttgatt caaaacttt gtttgtatt 
      gcactttgt ttccatagt aaaacggtg acaaagtca 
      atcgataaa taaacagac ctacaaaat aaacctgct 
      cgaaaatca aacgaatac atattgcaa ataattccc 
      gacatggat tttggggac gatttaggg gtgctatcc 
      gatggcagc aacgacagt ttctttgac gagcccaaa 
      ccggcatcc aaaagggca agtttgagc aaaacaccg 
      gaaaaaaag tccatcaca gatctattc aatataacc 
      gacaaaaca cccgaaagc aatcaagtg ccaaaacga 
      ccggacgac tggttggga ttagactcc agagacacc 
      aaatctcct tctcggagc caaagattg gccaaaaaa 
      atcagcttc gatgacgac gacgatatt ttgggcaat 
      ttgggtctg accaagaaa cctcagagt aaacccgaa 
      gaaaataaa acaccgacg aagaaaatg gatctgctt 
      gaaagtatt ttgggacca aaaagtgaa gatgttaaa 
      acagcgact tttgaggat attttgaaa gagagtaaa 
      gcaaaaact gtgactccg acgaccact ccaaagctg 
      acaccttca aatcctcca tcagagtct gtcttgttg 
      agcgaaggg cctcgggaa ggtcggcga aggcgtagg 
      tcttcggct ggattattg gaccctcta ggcttattt 
      agcaacgaa ataaaaact gatgattct gcttctaaa 
      gaaaaaata ataaccagc tctactgaa actcctgtt 
      tccaaacct acaccgtcg aaaagtgct ccaaacctg 
      tccgatcaa ggtcttccg gattggctt ggtggcgga 
      tccgcaatc aagcaaagc aaatcagaa aatatatta 
      ccagcaaaa caagaaccc gaaaaatcg atagaacca 
      gcaaaagaa agcgaccaa tcttccatt aacactatt 
      ttaacacag caaaagttg gcccagtcc cacatggag 
      tatcaaaat acggctatt gccctccaa cagcaagaa 
      tcgcaacta ctgatggcc ctccagttg aaaaaatac 
      gaagacaat ttatccgac atgcaaatg aagcaacag 
      gaagtttta atgaaacaa gagcaacag tttaatttg 
      cttctagaa aggcagttt gctaaacaa caaatcatg 
      gaaaataat atgcgcctt caacaagag agaattaat 
      aaccatatt caaatgcta ttagcgcaa ccaccaatt 
      ggcaaacat tcgggtcaa gaggaaatt gacgattta 
      aaaaagaca gcgagtgag gaaaacgtg aagctttac 
      gaaaacata atcaactcg ttgaaacag agacagcac 
      gaggaaatt ttcctactg gaggagtcc tacaagaaa 
      caaatcaat ttgttagaa caaagtctg gaatcagtc 
      gaaaggcgg ttgaaaact gacgttgaa aaaatgacc 
      gaagtgttt gaggaaaaa ttaaaaaca gtactacag 
      caacacgat ggtgaaatt gcaaaatac aagcaaaga 
      attgaagag gctgacgcg caccattcc gaagaaata 
      aaacggatt agagaaaat aattcccga gttattgag 
      gaaattaaa tacgaatac accaccctc ttggagaat 
      gtcaaagag gccaaaaaa tccgaatcg tcgttattc 
      caggaatcc aatacttac cttcagaaa ctggacagc 
      aatattgaa attctgtac gctaacagc aaaagtttg 
      ggggacctc aaagaccac atccagaag gattatggt 
      atattatcg cgggcgcgg gaagagtcc ttgaaagct 
      aaggagcag gagattatt ctgatgagg agtacgttg 
      gaaaaatgt cgcgaagaa gccgaaaag gagagagcg 
      gagcttttg gcgttggtg cgcaccttg gagactaaa 
      atatcggag caaaaccaa aatgcccgt gaagaaagg 
      tgggctttt cagcaagcg gctagcact ttagctgcg 
      cgctcagct gccatggac agagaagct gagtttaat 
      agagcagct ctcgaacgt gaaagagaa cagttaaag 
      actttgagg gaatcaatt ttggccgaa caagagaaa 
      accatgttg caactctcc gaggaaaaa ttatcgctc 
      gcagctgaa aaatcccgg ttcgaaacg tccaataaa 
      ttaatggtc aattacgac tcgcaaaag gccaaagcc 
      gaaatcgac gctgctgtg cagattgcg aaagaagcg 
      gccgaaatg gctgataaa gagcgggaa agtcttcat 
      aaagaaaaa tgcgagatg gaaaaacta aagcgatgt 
      ttgcaagac caggaacgt aagctgagt ttgagggaa 
      acagaagta gagaattta atgaaagaa gctcaaaag 
      aaggtaaac gaaggtgaa aaagccatt tttgagtcc 
      aaaactatc gaaaataag tacaacgaa agactgagg 
      gatttacaa aatcagcta aacatgtta tcgaataga 
      gaaaagaag ttagctgag gagaaaata gcgctcgct 
      aaggaacgt ttgtcattg aacactctc atgcgtcag 
      agtaagaag tgcagtctt tgcagtgct gatccacaa 
      aaacttgaa gaaagtcat cttaacata aactcggag 
      gttctgacg tcttatttt tcgagatat gataatatt 
      gacaatgac atccttcgt cttcgtttg gaagctact 
      gaggaaaat ctattgaac ataaaccac gatgtgcca 
      aactagaca atcatttgt aaggacttt tattgtttg 
      gtttgtacg tttataaaa aatacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY